• 제목/요약/키워드: Replicate

검색결과 849건 처리시간 0.028초

동력보행보조기의 보행시뮬레이션 (Gait Simulation of Powered Gait Orthosis)

  • 류제청;조현석;강성재;문무성
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 추계학술대회
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    • pp.1566-1571
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    • 2003
  • PGO(Powered Gait Orthosis) mounted with pneumatic muscle as an actuator is upgraded model from RGO(Replicate Gait Orthosis) for paraplegia patients to walk easy and safe. Pneumatic muscles supply powers to both hip joint during PGO gait. The objective of this research is to develop the PGO gait simulation model. Dynamic model of PGO linkage system is processed. Mathematical model of pneumatic muscle was developed and combined it with PGO linkage system. Developed simulation model will be used as a tool for evaluation of the efficiency of pneumatic muscle and for analysis the PGO system.

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Isolation and Characterization of a Cryptic Plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733

  • Chae, Han Seung;Lee, Jeong Min;Lee, Ju-Hoon;Lee, Pyung Cheon
    • Journal of Microbiology and Biotechnology
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    • 제23권6호
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    • pp.837-842
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    • 2013
  • A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.

고추씨의 영양적 가치 평가와 사료 내 첨가가 산란계의 생산성에 미치는 영향

  • 장익훈;노성래;권순관;김은집;임호중;강창원
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2001년도 제18차 정기총회 및 학술발표 PROCEEDINGS
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    • pp.54-55
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    • 2001
  • The experiments were conducted to evaluate the feeding values of red pepper seed (Exp. 1) and to investigate its dietary supplementation on layer performances (Exp. 2). The values of TME, TMEn and average TAAA determined by force-feeding 14 roosters (Hy-line) were 2.83 kcal/g, 2.94 kcal/g and 82.72%, respectively. a total of 200 layers (ISA-brown) was divided into 4 groups with 5 replicates of 10 birds per replicate, and fed the experimental diets containing different level of red pepper seed (0, 3, 5, 10%). There were no differences in performances of laying hens among treatments. Liver lipids in all groups containing red pepper seed were significantly reduced as compared to those of control (P<0.05).

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Relationship Between Plant Viral Encoded Suppressor to Post-transcriptional Gene Silencing and Elicitor to R Gene-specific Host Resistance

  • Park, Chang-Won;Feng Qu;Tao Ren;T. Jack Morris
    • The Plant Pathology Journal
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    • 제20권1호
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    • pp.22-29
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    • 2004
  • Many important horticultural and field crops are susceptible to virus infections or may possess a degree of resistance to some viruses, but become infected by others. Plant viruses enter cells through the presence of wounds, and replicate intracellularly small genomes that encode genes required for replication, cell-to-cell movement and encapsidation. There are numerous evidences from specific virus-host interactions to require the involvement of host factors and steps during viral replication cycle. However, viruses should deal with host defense responses either by general or specific mechanisms, targeting viral components or genome itself. On the other hand, the host plants have also adapted to defend themselves against viral attack by operating different lines of resistance responses. The defense-related interactions provide new insights into the complex molecular strategies for hosts for defense and counter-defense employed by viruses.

SPL과 소프트 리소그래피를 이용한 나노 구조물 형성 연구 (Fabrication of Nanoscale Structures using SPL and Soft Lithography)

  • 류진화;김창석;정명영
    • 한국정밀공학회지
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    • 제23권7호
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    • pp.138-145
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    • 2006
  • A nanopatterning technique was proposed and demonstrated for low cost and mass productive process using the scanning probe lithography (SPL) and soft lithography. The nanometer scale structure is fabricated by the localized generation of oxide patterning on the H-passivated (100) silicon wafer, and soft lithography was performed to replicate of nanometer scale structures. Both height and width of the silicon oxidation is linear with the applied voltagein SPL, but the growth of width is more sensitive than that of height. The structure below 100 nm was fabricated using HF treatment. To overcome the structure height limitation, aqueous KOH orientation-dependent etching was performed on the H-passivated (100) silicon wafer. Soft lithography is also performed for the master replication process. Elastomeric stamp is fabricated by the replica molding technique with ultrasonic vibration. We showed that the elastomeric stamp with the depth of 60 nm and the width of 428 nm was acquired using the original master by SPL process.

산란계에서 High Oil Corn의 사료가치 평가

  • 이봉덕;김영호;김동준
    • 한국가금학회:학술대회논문집
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    • 한국가금학회 2000년도 제17차 정기총회 및 학술발표
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    • pp.108-110
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    • 2000
  • A 12-w feeding trial was conducted with 600 38-wk-old layers to evaluate the nutritive value of high oil corn(HOC) in layer diets. For the control, a commercial corn-soybean meal basal diet was used. An iso-calorie diet(HOC-ISO) was prepared replacing corn with HOC in the control diet. In addition a third diet was prepared by simply replacing corn with HOC on 1:1 basis to give a high energy diet(HOC-1:1). There were 5 replicates per treatment, and 40 birds per replicate. Birds were housed in an open-sided layer house equipped with wire cages. Feed and water were provided ad libitum, and 17L:7D lighting program was adopted. No significant difference (P>0.05) was found among dietary treatments in laying performance. Also physical qualities of eggs were not significantly affected by the feeding of HOC. Although not significant, HOC-ISO tended to improve the laying rate during the summer. The HOC feeding significantly increased the polyunsaturated fatty acids contents in egg yolk, especially the linoleic acid The AME contents of Chinese yellow corn, U.S. corn, and HOC were 3177, 3158 and 3387 kcal/kg respectively.

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Spectrophotometric Determination of Nickel in Steel with Di-2-pyridylmethanone 2-Thiophenecarboxylic Hydrazone

  • Nakanishi, Tsutomu
    • 분석과학
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    • 제8권4호
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    • pp.469-475
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    • 1995
  • Di-2-pyridylmethanon 2-thiophenecarboxylie hydrazone(DPMTCH) was synthesized and used as a chromogenic reagent in the spectrophotometric determination of nickel in steel. The proton disc spectrophotometrically, were $pK_{a1}= 2.66$ and $pK_{a2}= 10.98$ ${\mu}=0.5(NaCl)$. Nickel(II) reacts with DPMTCH to form a 1:2 metal-2-ligand ratio complex, the xylene solution of which has an absorption maximum at 417 nm. Beer's law is obeyed over the range $0-1.17\;mg\;ml^{-1}$ of nickel(II). The molar absorptivity of the nickel(II) complex is $4.17{\times}10^4l\;mol^{-1}\;cm^{-1}$. The precision of the method was estimated for 4.7 mg of nickel(II), the coefficient of variation for 15 replicate determinations being 0.34%.

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Deep learning for stage prediction in neuroblastoma using gene expression data

  • Park, Aron;Nam, Seungyoon
    • Genomics & Informatics
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    • 제17권3호
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    • pp.30.1-30.4
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    • 2019
  • Neuroblastoma is a major cause of cancer death in early childhood, and its timely and correct diagnosis is critical. Gene expression datasets have recently been considered as a powerful tool for cancer diagnosis and subtype classification. However, no attempts have yet been made to apply deep learning using gene expression to neuroblastoma classification, although deep learning has been applied to cancer diagnosis using image data. Taking the International Neuroblastoma Staging System stages as multiple classes, we designed a deep neural network using the gene expression patterns and stages of neuroblastoma patients. Despite a small patient population (n = 280), stage 1 and 4 patients were well distinguished. If it is possible to replicate this approach in a larger population, deep learning could play an important role in neuroblastoma staging.

인공신경망기법을 이용한 깊은 굴착에 따른 지표변위 예측 (Prediction of Deep Excavation-induced Ground Surface Movements Using Artificial Neural Network)

  • 유충식;최병석
    • 한국지반공학회논문집
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    • 제20권3호
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    • pp.53-65
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    • 2004
  • 본 연구에서는, 굴착 공사로 인한 주변 건물 손상 평가의 일환으로 지표 변위유형의 효과적인 예측 방안을 제시하였다. 먼저 검증된 유한요소 모델을 국내에서 행해지는 다양한 경우의 굴착특성으로 해석한 결과를 통해 인접 지반의 거동에 대한 매개 변수 연구를 수행하였고, 인공신경망 엔진의 학습을 위한 데이터베이스를 구축하였다. 최적의 구조로 학습된 신경망 엔진은 간단한 굴착 특성으로 다양하게 나타나는 지표 변위 유형 예측에 효율적임을 검증하였다.

재조사에서 효율 향상을 위한 추정법 연구 (Estimation to improve survey efficiency in callback)

  • 박현아;나성룡
    • Journal of the Korean Data and Information Science Society
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    • 제26권2호
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    • pp.377-385
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    • 2015
  • 표본조사에서 무응답이 발생한 개체에 대해 재조사 실시한 후 보조변수를 사용한 회귀추정의 형태를 가지는 추정량을 제시하고 복제치 기법을 이용한 분산추정량을 연구한다. 또한 응답여부에 따른 응답확률의 모수적 추론방법도 함께 제시한다. 재조사 후 모평균에 대하여 불편성을 만족하고 효율이 좋은 추정량과 일치성을 가지는 분산추정량을 이론적으로 연구하고 모의실험을 통하여 연구의 타당성을 입증한다.