• Journal of Life Science
• /
• v.22 no.12
• /
• pp.1608-1613
• /
• 2012

A conventional kinesin, KIF5/Kinesin-I, transports various cargoes along the microtubule through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) interact with many different cargoes using their tetratricopeptide repeat (TPR) domain, but the mechanism underlying recognition and binding of a specific cargo has not yet been completely elucidated. We used the yeast two-hybrid assay to identify proteins that interact with the TPR domain of KLC1. We found an interaction between the TPR domain of KLC1 and an amyloid precursor protein (APP)-binding protein PAT1 (protein interacting with APP tail 1). The yeast two-hybrid assay demonstrated that the TPR domain-containing region of KLC1 mediated binding to the C-terminal tail region of PAT1. PAT1 also bound to KLC2 but not to kinesin heavy chains (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. These protein-protein interactions were also observed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti-PAT1 antibody as well as anti-APP anti-body co-immunoprecipitated KLC and KHCs associated with PAT1 from mouse brain extracts. These results suggest that PAT1 could mediate interactions between Kinesin-I and APP containing vesicles.

• Biomedical Science Letters
• /
• v.17 no.2
• /
• pp.151-155
• /
• 2011

Short tandem repeats (STRs) loci are the genetic markers used for forensic human identity test. With multiplex polymerase chain reaction (PCR) assays, STRs are examined and measured PCR product length relative to sequenced allelic ladders. In the repeat region and the flanking region of the commonly-used STR may have DNA sequence variation. A mismatch due to sequence variation in the DNA template may cause allele drop-out (i.e., a "null" or "silent" allele) when it falls within PCR primer binding sites. The STR markers were co-amplified in a single reaction by using commercial PowerPlex$^{(R)}$ 16 system and AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI PRISM$^{(R)}$ 3100 Genetic Analyzer with capillary electrophoresis. The GeneMapper$^{TM}$ ID software were used for size calling and analysis of STR profiles. Here, this study described a forensic human identity test in which allelic drop-out occurred in the STR system D18S51. During the course of human identity test, two samples with a homozygous (16, 16 and 21, 21) genotype at D18S51 locus were discovered using the PowerPlex$^{(R)}$ 16 system. The loss of alleles was confirmed when the samples were amplified using AmpFlSTR$^{(R)}$ Identifiler$^{(R)}$ PCR amplification kit and resulted in a heterozygous (16, 20 and 20, 21) genotype at this locus each other. This discrepancy results suggest that appropriate measures should be taken for database comparisons and that allele should be further investigated by sequence analysis and be reported to the forensic community.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.6 no.3
• /
• pp.156-161
• /
• 2013

Gas valve time control system used to set the time to use the gas system by one of the user's negligence explosion, fire, and to prevent accidents such as suffocation. Gas valve is one of the items is not a replacement without any special issue after installation in each customer. So sufficient test is required prior to the installation device. In this study, the test system was developed to use to set the number of times at the gas valve time control system.

• Journal of Industrial Technology
• /
• v.22 no.B
• /
• pp.241-249
• /
• 2002

Optical fiber is indispensable for optical communication systems that transmit large volumes of data at high speed, but super precision technology in sub-micron units is required for optical axis adjustment. We developed the automatic optical fiber by image processing and automatic loading system. we have developed 6-axis micro stage system for I/O optical fiber arrays, the initial automatic aligning system software for a input optical array by the image processing technique, fast I/O-synchronous aligning strategy, the automatic loading/unloading system and the automatic UV bonding mechanism. In order to adjust the alignment it used on PC based motion controller, a $10{\mu}m$ repeat-detailed drawing of automatic loading system is developed by a primary line up for high detailed drawing. Also, at this researches used the image processing system and algorithm instead of the existing a primary hand-line up and fiber input array and waveguide chip formed in line by automatic.

• Journal of the military operations research society of Korea
• /
• v.31 no.2
• /
• pp.105-115
• /
• 2005

To ensure interoperability among TADILs, we need inter-operational system of tactical data link that allows sharing of specific, planned information among different TADILs. In this paper, we have proposed the design of a conceptual software architecture of inter-operational system. For developing of a conceptual software architecture, we analyze the actual condition of ADSI used in the US military and identify functions and quality-attributes. Based on these factors, we design the conceptual software architecture for inter-operational system of tactical data link using Attribute-Driven Design(ADD) method. ADD is consist of three phases - choose the module to decompose, refine the module, repeat the steps for every module that needs further decomposition. To evaluate of ADD results, we apply the Software Architecture Analysis Method(SAAM) which is consist of making evaluation scenarios, choosing indirect scenarios, evaluation scenarios' interaction, and creating an overall evaluation. Through the evaluation, we verify the conceptual software architecture of inter-operational system.

• Proceedings of the IEEK Conference
• /
• 1998.06a
• /
• pp.11-14
• /
• 1998

This paper introuces a new efficient method of synchronization acquisition which is the most important element in DS-CDMA system using the phase offset of binary code. This approach uses the binary code function which can easily estimate the phase offset from the received spreading waveforms which respect to the receiver-stored replica of the spreading code. This paper proposes the initial acquisition model with repeat error control device that is good for perfomance. The hardware is implemented by TMS320c30

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.21 no.3
• /
• pp.731-736
• /
• 1996

In this paper, we propose a modified Type-II HARQ system having adaptive power to channel and analyze its performance in terms of throughput and reliability. Its performance is also compared with Typd-I and Type-II HARQ ystems and is verified using computer simulations over AWGN and frequenyc-nonselective, slowly Rayleigh fading channels.

• Proceedings of the IEEK Conference
• /
• 2006.06a
• /
• pp.1043-1044
• /
• 2006

In a packet based wireless system, an adaptive modulation and coding (AMC) scheme is often employed with the use of channel quality information (CQI). However, the AMC can be operated improperly due to delayed CQI, causing performance degradation. In this paper, we consider the use of predicted CQI for the AMC considering the feedback delay and channel characteristics. We also consider the use of a hybrid automatic repeat request scheme with the proposed AMC for further improvement in high mobility environments.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• v.9 no.2
• /
• pp.246-249
• /
• 2005

본 논문에서는 4세대 이동통신을 위한 적응부호화 하이브리드 ARQ 시스템 모델을 제안하고, 제안 시스템의 성능을 분석 검토 하였다. 또한 적응 하이브리드 시스템의 프레임 효율도 분석하였다. 결과로부터 제안 시스템이 성능이나 효율에서 우수함을 확인 할 수 있었다.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.32 no.4C
• /
• pp.370-378
• /
• 2007

The most appropriate low density parity check (LDPC) code for hybrid automatic repeat request (HARQ) system suitable for future multimedia communication systems is presented in this paper. HARQ system with punctured LDPC code is investigated at first. And two transmission schemes with parallel concatenated LDPC code are also presented and their performances are analyzed according to the various values of mean column weight (MCW). As a result, the parallel concatenated LDPC code with the diversity effect of information bit is considered to be more appropriate for HARQ system considering the throughput as well as error performance.