• Journal of Nutrition and Health
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• v.48 no.2
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• pp.133-139
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• 2015

Purpose: There is a fair amount of evidence indicating that increased risk of obesity and insulin resistance is associated with postmenopausal state, but can be modulated by diet and exercise. In this study, we explored whether a Pueraria lobata root-based supplement containing Rehmannia glutinosa (PR) and/or aerobic treadmill exercise can modify the metabolic changes associated with estrogen deficiency. Methods: Seventy rats were randomly assigned to the following groups for 8 weeks (n=10 per group): SHAM, sham-operated; PR0, ovariectomized (OVX) control; PR200, OVX with PR200 mg/kg B.W; PR400, OVX with PR400 mg/kg B.W; EPR0, OVX with exercise; EPR200, OVX with exercise and PR200 mg/kg B.W; EPR400, OVX with exercise and PR400 mg/kg B.W. Results: OVX induced significant increases in body weight, food intake, fat mass, LDL-cholesterol, and fasting blood glucose, confirming induction of menopausal symptoms. PR supplementation or exercise significantly suppressed the above mentioned changes through different regulatory elements in adipose tissue: PR supplement upregulated adiponectin gene expression and aerobic exercise upregulated adiponectin and insulin receptor gene expression and a combination of PR supplement and aerobic exercise showed an additive effect on adiponectin gene expression. Conclusion: Taken together, the results of this study suggest that PR supplement has a potential to provide health benefits in OVX rats through leptin and adiponectin secretion. In addition, the data suggest that combination of exercise and PR would have additive effects on metabolic dysfunction associated with estrogen deficiency.

• Korean Journal of Medicinal Crop Science
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• v.3 no.2
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• pp.120-124
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• 1995

This experiment was carried out to elucidate the effect of planting depthon the quality and yield in Rehmannia glutinosa . Planting depths were 3, 6, 9 and 12cm, and planted in April 18. Emergence rate were decreased in the order of 86 % in 3cm, 67 % in 6cm, 37 % in 9cm and 21 % in 12cm. Leaf length was longer about 0.3 - 0.8cm in planting depth $6{\sim}12cm$ than that of 15.6cmin planting depth 3cm. Yield of Rehmannia glutinosa were lower 5% in plantingdepth 6cm, 62% in 9cm and 68% in 12cm than that of 1,154kg/10a in planting depth 3cm. And optimum planting depth were considered 6cm.

• Korean Journal of Soil Science and Fertilizer
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• v.22 no.2
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• pp.105-110
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• 1989

The purpose of this study was centered on investigation of the effects N, P and K application rates on root yield and their available content (catalpol, sugar, ash and extracts) of Ji-whang(Rehmannia glutinosa Lib. var. purpurea Makino), and the current application rates(N 20kg/10a, $P_2O_5\;30kg/10a$, $K_2O\;20kg/10a$) was studied for Ji-whang production in Eui-sung Gun southeastern part of Korea Peninsular. The yields of fresh and dried rhizomas were 941-1,494 kg/10a and 251-385 kg/10a, respectively. The rhizoma yield increased highly with increasing the application amount of nitrogen more than 10 kg/10a, but the effects of phosphorus and potassium were observed only at the application level of 40 kg/10a and 20 kg/10a, respectively. Catalpol contents in rhizoma increased at the plot of 20 kg/10a application of nitrogen, but the effects phosphorus and potassium on the catalpol contents were not observed. Catalpol content in dried root increased with increasing N application more than 10 kg/10a. Fructose contents increased at the plot of 20 kg/10a and more N application, and galactose contents decreased with K application. With increasing N application, ash contents decreased, but ethanol extract contents increased.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.12-15
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• 1995

This experiment was carried out to elucidate the effect of planting depth on the Quality and yield in Rehmannia glutinosa. Planting depths were 3,6,9 and 12cm, and planted on April 18. Emergence rate was decreased in the order of 86% in 3cm, 67% in 6cm, 37% in 9cm and 21% in 12cm. Leaf length was longer about $0.3{\sim}0.8cm$ in planting depth 6-12cm than that of 15.6cm in planting depth 3cm. Yield of Rehmannia glutinosa were lower 5% in planting depth 6cm, 62% in 9cm and 68% in 12cm than that of l,154kg/10a in planting depth 3cm. And optimum planting depth was considered 6cm.

• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.94-99
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• 1997

When the leaf and stem tissues of Rehmannia glutinosa were cultured on MS medium with plant growth regulators, shoots were regenerated on MS medium with TDZ(0.01 to $2.0{\mu}M$), and root initiation was better on MS medium treated with NAA(0.01 to 2.0mg/$\iota$). On $B_5$ medium, shoot regeneration was better on medium with TDZ than those with Quincrac, NAA, and 2.4-D. The addition of Quincrac, NAA, and 2.4-D inhibited shoot regeneration on MS medium, but promoted shoot regeneration on $B_5$ medium. Shoot regeneration and growth was better on MS medium with the combination treatments of 2.4-D 0.01mg/$\iota$ and TDZ 0.01, 0.1 and $2{\mu}M$ compared with other treatments. Also shoot regeneration and growth on B_5 medium showed the similar results to that on MS medium.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.273-277
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• 2005

Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1 $mg/{\ell}\;BA\;and\;2\;mg/{\ell}$ IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1 $mg/{\ell}\;IBA$. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.

• Korean Journal of Pharmacognosy
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• v.31 no.2
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• pp.130-141
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• 2000

The dried roots of Rehmannia glutinosa (RG) have been used in the traditional medicine for treatment of diabetes, fever and dysuria, etc. In order to investigate the quality evaluation of the dried roots of RG, we conducted the physico-chemical and biological evaluation method. The amount of catalpol from commercial samples is very diverse about $0.00{\sim}3.89%$ by using HPLC method, because it is easily decomposed by processing of RG. So, we should try to identify the correlation with the contents of catalpol and biological activities of RG. We chose 3 samples which were a wide difference of catalpol contents between each sample (Sample-I; 3.4%, Sample-II; 2.8%, Sample-III; 0.05%). Sample-I and Sample-II were found to be more effective than Sample-III on the DPPH free radical scavenging effect and inhibitory effect on $H_2O_2-catalyzed$ lipid peroxidation in rat liver in vitro. And, Sample-I and Sample-II exhibited more significant effects than Sample-III on accelerating actions of the small and large intestinal transport, and diuretic action in mice. So, it is suggested that the quantitative determination of catalpol should be required for the tandardization of the dried roots of RG.

• Korean Journal of Medicinal Crop Science
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• v.9 no.4
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• pp.295-300
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• 2001

This experiment was conducted to evaluate the germination rate according to temperature, pelleting materials, pelleted size, seed storage periods under light and dark conditions in Rehmannia glutinosa. The highest germination rate was obtained from $25^{\circ}C$ in both light and dark condition. Two-year old seeds showed higher germination rate compared to one-year old seed. Talc material showed the highest germination rate among the pelleting materials by 82.5%. Among the different pelleting sizes, the highest germination rate was shown in 2.0 mm, and especially Talc pelleting showed 100% germination rate on 17days after seeding. In case of combination of pelleting materials, both of talc + vermiculite, zeolite + vermiculite showed lower germination rate than the control and 0.7 mm pelleted seed showed the highest germination rate.

• Korean journal of food and cookery science
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• v.30 no.1
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• pp.76-83
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• 2014

In this study, we have investigated the quality characteristics and antioxidant activities of Rehmannia glutinosa JungKwa (RJ) prepared with different types of sugars (sucrose, isomalto-oligosaccharide, honey or sorbitol). Moisture content was the highest in RJ with honey. The pH level was the highest in RJ with sorbitol, whereas the acidity is the highest in honey. The reducing sugar content is the highest in RJ with honey. The L and a values of Hunter color system showed the highest in RJ with honey, while the b value the highest in RJ with sorbitol. The hardness of texture profile analyses represented the lowest in RJ with sorbitol. Sensory results showed that RJ with sugar was the best in the sensory preference test. The total phenol content was the highest in RJ with honey, while the lowest in RJ with sorbitol. The antioxidant activity determined by $IC_{50}$ values of DPPH and hydroxy radical scavenging activities was the highest in RJ with honey. From these results, it was suggested that RJ with honey show the highest antioxidant activity.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.273-276
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• 1998

Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the crude drug damand expands rapidly. This study was conducted to obtain the basic breeding information of Chinese foxglove. Meristem culture was employed for virus free seedling production and miropropagation. Both Jiwhang 1 and domestic local were severely infected by potexvirus and TMV. Several growth regulators were used for the virus free stock production from Jiwhang 1 and Danyang local. Shoot formation and callus induction from the meristem culture seemed to be influenced by the content of various kinds of plant growth regulators. Kinetin supplement was the most effective on shoot formation and NAA addition was good on callus induction among the treatments. The acquired virus free stocks were confirmed using transmission electron microscope and indicate plants.