Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Efficient Procedures for Direct Shoots Regeneration from Leaf Explants of Rehmania glutinosa Lib.

지황 잎조직 절편으로부터 신초 형성

  • Hwang, Sung-Jin (Department of Oriental Medicine Materials, Dongshin University)
  • 황성진 (동신대학교 한약재산업학과)
  • Published : 2005.12.30
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Abstract

Adventitious shoots were directly induced from leaf explants of R. glutinosa, an important medicinal plant. Proliferating shoot cultures were obtained by culturing leaf discs on Murashige and Skoog(MS) medium alone or combination with auxins and cytokinins. MS medium supplemented with 1 $mg/{\ell}\;BA\;and\;2\;mg/{\ell}$ IAA was the most effective, providing high shoot bud formation frequency without formation of intervening callus. The effect of leaf age on adventitious shoot formation was also investigated. The maximum shoot bud production (93.4%) was achived using 3rd leaf from apex of 6 weeks old plantlets after seed germination. Plantlet were rooted on an half-strength MS (1/2MS) medium containing 0.1 $mg/{\ell}\;IBA$. This prtocol is useful for clonal propagation and Agrobacterium-mediated transforamtion in R. glutinosa.

지황 (R. glutinosa)의 조직절편으로부터 고효율의 식물체 재분화 시스템을 확립하기 위하여 기내에서 발아된 유묘의 잎을 절취하여 auxins과 cytokinins을 농도별로 단독 또는 혼합하여 MS배지에 치상한 후 신초의 형성율을 조사하였다. 잎 절편으로부터 부정아의 형성율은 1 mg/{\ell}\;BA와\;2mg/{\ell}$ IAA가 첨가된 MS배지에서 93.4%로 가장 높았다. 한편, 엽령에 따른 부정아의 형성율을 조사한 결과 기내에서 발아 후 6주가 지난 유식물체의 정단부위로부터 3번째의 잎을 사용함으로써 신초의 형성율을 98.4%까지 높일 수 있었다. 형성된 신초는 $0.1mg/{\ell}$ IBA가 첨가된 1/2MS배지로 옮겼을 때 4주 후 최대 발근율을 보여주었다.

Keywords

References

  1. vitro Cell Dev. Biol.-Plant v.36 Regeneration of plantlet from leaf and petiole explants of P. hortorum Agarwal, P.K.;Ranu, R.S.
  2. Biotechnology of micropropagation of medicinal and aromatic plants;Biotechnology in agriculture and forestry Bajaj, Y.P.S.;Furmanowa, M.;Olszowska, O.
  3. Plant tissue culture : theory and practice Bhojwani, S.S.;Razdan, M.K.
  4. vitro Cell Dev. Biol.-Plant v.40 Micropropagation of F carica Fraguas, C.B.;Pasqual, M.;Dutra, L.F.;Cazetta, J.O.
  5. Plant Cell, Tiss. Org. Cult. v.6 Factors affecting plant regeneration from leaf segments of A. scherzerianum cultured in vitro Geier, T.
  6. In Plant propagation by tissue culture. part I. The technology George, E.F.
  7. In Plant propagation by tissue culture Gorge E.F.;Sherrington, P.D.
  8. vitro Cell Dev. Biol-Plant v.38 Plant regeneration from excised hypocotyl explants of P. acerifolia Guofeng, L.;Haung, J.;Chen, L.;Bao, M.
  9. RDA Res. Bull. Fundamental studies on plant breeding Han, C.Y.
  10. vitro Cell Dev. Biol.-Plant v.40 Micropropagation of S. caucasica Hosoki, T.;Nojima, S.
  11. Chinese Med. Herb. Lett. v.2 Callus formation and plantlet regeneration of R. glutinosa Jiang, L.C.;Mao, W.Y.
  12. Sci. Hort. v.96 High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of D. chinensis Kantia, A.;Kothari, S.L.
  13. Theor. App. Genet. v.60 Somaclonal variation-a novel source of variability from cell cultures for plant improvement Larkin, J.;Scowcroft, W.R.
  14. Physiol. Plant v.15 Cultivation of medicinal crops Lee, S.T.;Chae, Y.A.
  15. vitro Cell Dev. Biol.-Plant v.41 Plant regeneration from leaf petiole in C. acuminata Li, Z.;Liu, Z.
  16. vitro Cell Dev. Biol.-Plant v.38 Plant regeneration from excised hypocotyl explants of P. acerifolia Liu, G.;Huang, J.;Chen, L.;Bao, M.
  17. Plant Cell Rep. v.21 Regeneration of the Egyptian medicinal plant A. judaica Liu, C.Z.;Murch, S.J.;Demerdash, M.E.L.
  18. Sci. Hort. v.72 Factors affecting shoot organogenesis in leaf disc culture of African violet Lo, K.H.
  19. vitro Cell Dev. Biol.-Plant v.37 Direct shoot organogenesis and plant regeneration in safflower Mandal, A.K.A.;Gupta, S.D.
  20. Hort. Sci. v.23 Paulownia tomentosa Somaclonal Snowstorm Marcotrigiano, M.;Jagannathan, L.
  21. vitro Cell Dev. Biol.-Plant v.37 Direct differentiation of shoot buds in leaf segments of T. erecta Misra, P.;Datta, S.K.
  22. Plant Physiol. v.15 A revised medium for rapid growth and bioassay with tobacco tissue cultures Murashige, T.;Skoog, F.
  23. NATO ASI series v.210 Effect of explant and media on initiation, maintenance, and maturation of somatic embryos in P. menziesii Nagmani, R.;Johnson, M.A.;Dinus, R.J.
  24. Plant Cell Rep. v.10 Plant regeneration from stem and petal of carnation Nugent, G.;Wardely-richardson, T.;Lu, C.Y.
  25. Biotech. Lett. v.24 Agrobacterium mediated genetic transformation of R. glutinosa Park, S.U.;Kim, H.H.;Yu, C.Y.;Park, C.H.;Chae, Y.A.
  26. vitro Cell Dev. Biol.-Plant v.36 Shoot regeneration of young leaf explants from O. basilcum Phippen, W.B.;Simon, J.E.
  27. vitro Cell Dev. Biol.-Plant v.38 Thidiazuron promotes adventitious shoot regeneration from E. aureum leaf and petiole explants Qu, L.;Chen, J.;Henny, R.J.;Haung, Y.;Caldwell, R.D.;Robinson, C.A.
  28. vitro Cell Dev. Biol.-Plant v.38 Rapid clonal propagation of N. foetida Rai, V.R.
  29. vitro Cell Dev. Biol.-Plant v.36 In vitro differentiation and plant regeneration of A. chinenesis Sinha, R.K.;Majumdar, K.;Sinha, S.
  30. Sci. Hort. v.51 Adventitious shoot formation from in vitro leaf explants of D. caryophyllus Von Alvorst, A.C.;Koehorst, H.J.J.;Bruinsma, T.;Jansen, J.;Custer, J.B.M.;Jong, J.;Dons, J.J.J.
  31. Plant Cell, Tiss. Org. Cult. v.37 Improvement of adventitious shoot formation from carnation leaf explants Von Alvorst, A.C.;Koehorst, H.J.J.;Bruinsma, T.;Dons, J.J.J.
  32. Transgenic Res. v.4 Transgenic carnations obtained by A. tumefaciens mediated transformation of leaf explants Von Alvorst, A.C.;Riksen, T.;Koehorst, H.J.J.;Dons, J.J.J.