• Journal of agriculture & life science
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• v.50 no.5
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• pp.81-93
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• 2016

Rehmannia glutinosa(Gaertn.) DC. is a herbaceous perennial plant and belonging to the Scrophulariaceae and used as roots for medicinal part and purpose. R. glutinosa is and usually used for fresh rehmannia root or prepared rehmannia root. However, it is very difficult to propagate using the seeds because of lack germination so it is propagated using the vegetative method as the rootstock. Currently, propagation and harvesting using the rootstock of R. glutinosa has difficulties about production of the high quality and quantity in R. glutinosa because of root rot disease. To optimize in vitro cultures and to improve the rootstock and seedling of R. glutinosa after morphological and genetical determination, 5 plant culture media (MS, DJ, LS, QL, and WPM) were used in this study then WPM was selected for better growth, for multiplication condition(WPM + IAA 1.0 mg/L + IBA 0.5 mg/L), and for root enlargement condition(WPM + NAA 0.1 mg/L) of R. glutinosa. Based on these results, in vitro seedlings of R. glutinosa were transferred to soil for acclimation with environment adaptation and shown the positive effects about root enlargement and root formation. Therefore, it can be used for high quality of R. glutinosa production and production of the rootstock based on propagation using in vitro seedlings of R. glutinosa.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.190-198
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• 2007

Alzheimer's disease, a representative neurodegenerative disorder, is characterized by the presence of senile plaques and neurofibrillary tangles accompanied by neuronal damages. b-Amyloid peptide is considered to be responsible for the formation of senile plagues that accumulate in the brains of patients with Alzheimer's disease. There has been compelling evidence supporting that b-amyloid-induced cytotoxicity is mediated through generation of reactive oxygen species. In this study, we have investigated the possible protective effect of Rehmannia glutihosaagainst b-amyloid-induced oxidative ceil death in cultured human neuroblastoma SH-SY5Y cells. SH-SY5Y cells treated with b-amyloid underwent apoptotic death as determined by morphological features and positive in situterminal end-labeling (TUNEL staining). Rehmannia glutinosawater extract, wine, and vinegar pretreatments attenuated b-amyloid-induced cytotoxicity and apoptosis. Rehmannia glutinosa vinegar exhibited maximum protective effect by increasing the expression of anti-apoptotic protein, Bcl-2. in addition to oxidative stress, b-amyloid-treatment caused nitrosative stress via marked increase in the levels of nitric oxide, which was effectively blocked by Rehmannia glutinosa. To further explore the possible molecular mechanisms underlying the protective effect of Rehmannia glutinosa, we assessed the mRNA expression of cellular antioxidant enzymes. Treatment of Rehmannia glutinosa vinegar led to up-regulation of heme oxygemase-1 and catalase. These results suggest that Rehmannia glutinosa could modulate oxidative neuronal cell death caused by b-amyloid and may have preventive or therapeutic potential in the management of Alzheimer's disease. Particularly, Rehmannia glutinosa vinegar can augment cellular antioxidant capacity, there by exhibiting higher neuroprotective potential.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.221-226
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• 1998

This study was conducted to develop labor-saving cultural techniques for weed control using herbicides in Rehmannia glutinosa and Astragalus membranaceus, Napropamide, linuron(preemergence) and paraquat (postemergence) showed significant herbicidal effects. At the same time they did not turn out to be harmful to the growth of Rehmannia glutinosa. The yield of Rehmannia, glutinosa treated with paraquat was higher than that of the untreated. Napropamide showed significant herbicidal effect without harmful effects to the growth of Astragalus membranaceus. Etha1flura1in and metolachlor, however, were s1ightly harmful. The yield of Astragalus membranaceus was highest with napropamide.

• Applied Biological Chemistry
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• v.32 no.3
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• pp.249-254
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• 1989

This study was conducted to clarify the relationships between the inorganic constituents contents and the contents of catalpol and free sugars in the rhizoma of Rehmannia glutinosa. Nitrogen contents in rhizoma was positively correlated with the contents of catalpol and ethanol extract, whereas it was negatively correlated with that of ash, galactose and fructose. Calcium contents in rhizoma was highly negatively correlated with the contents of catalpol, ethanol extract and water extract. However, the calcium contents positively correlated with that of ash, acid insoluble ash, fructose and galactose in R. glutinosa. The catalpol cotents was negatively correlated with the contents of ash, galactose and fructose, whereas positively correlated with that of ethanol extract and water extract, and the correlations between extract contents and ash contents showed highly negative significance.

• Current Research on Agriculture and Life Sciences
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• v.11
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• pp.19-23
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• 1993

This study was conducted to investigate contents of inorganic constituents, free sugars and catalpol in the Rhizoma of Rehmannia glutinosa as different growth stages. With the approach of harvesting season, the variations of their contents were as follow. The contents of P, K, Ca, Mg, Cu and ash in the Rhizoma decreased, whereas the contents of Fe, water and ethanol extract increased. The contents of total sugar, sucrose and galactose in the Rhizoma decreased, but the fructose and catalpol contents in the Rhizoma were increased.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.2
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• pp.5-11
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• 2023

Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae and is used in traditional oriental medicine. This study was carried out current status of breeding of R. glutinosa, its problems, and the matters to be improved were made. Based on the research data published so far, this study summarized the characteristics of breeds cultivated in Korea and compared and analyzed the status of cultivation area, production, import, and price changes for 15 years from 2008 to 2021. The cultivated area of R. glutinosa is gradually decreasing, and the amount of import is increasing and decreasing repeatedly. Amount of import has declined significantly. There was a lot of disinfection and disposal in the middle of import, and dried Jihwang and Sukjihwang were imported without distinction. R. glutinosa must be cultivated and supplied as a variety desired by the industry, cultivation techniques need to be taught to producers. In order to clearly identify the amount of import, import code of dried Jihwang and Sukjihwang must be classify. In addition, it is necessary to develop and supply harvesting agricultural machines for mechanization. For the stable income of producers, supply control through contract cultivation will ensure stable farm income.

• Research in Plant Disease
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• v.25 no.1
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• pp.38-42
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• 2019

While rehmannia (Rehmannia glutinosa Libosch) was identified as a host of at least five viruses, including Rehmannia mosaic virus (ReMV), Youcai mosaic virus (YoMV), Broad bean wilt virus 2 (BBWV2), Plantago asiatica mosaic virus (PlAMV), and Rehmannia virus 1 (ReV1), viral incidence surveys have not been performed yet in rehmannia fields in Korea. In this study, we performed field surveys during 2017-2018 to investigate the incidence of 5 major viruses in rehmannia. A total of 145 symptomatic samples were collected from the rehmannia fields in major cultivation areas of Korea. Molecular diagnosis assays showed that all the collected leaf samples were infected with more than two viruses. Particularly, two species of Tobamovirus, ReMV and YoMV, were detected in all the samples. In addition, our analysis showed that the root stocks of 4 rehmannia cultivars were infected with at least two viruses. Since rehmannia is propagated by vegetative propagation, it is highly important to produce virus-free root stocks of rehmannia to control virus diseases in rehmannia.

• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.138-142
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• 1999

Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the demand for crude drug expands rapidly. This study was conducted to obtain the basic agronomic characteristics and cultivation information of Chinese foxglove. Morphological traits of several Chinese foxglove and their plant growth and yield were investigated under different environmental conditions. The tested lines exhibited clear morphological differences in leaves and roots representing their origins. Rapid root growth and weight increasement occurred in the middle of July. Optimum daylength and temperature conditions were investigated for the adequate plant growth of Chinese foxglove. Root growth was enhanced at $23/18^{\circ}C$ (day/night) with 13 hours daylength condition. Appropriate soil moisture and soil texture were $60{\sim}70%$ and loam soil, respectively.

• Applied Biological Chemistry
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• v.41 no.7
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• pp.505-509
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• 1998

Chitinase, which is one of the pathogenesis-related proteins, was examined from Rehmannia glutinosa. Rehmannia chitinases were classified into basic and acidic groups by chitin affinity column chromatography. According to the chitinase pattern by native PAGE, basic chitinases of low Rf values were extracted more under an acidic condition (pH 2.9) than a basic condition (pH 8.8), while acidic chitinases having high Rf values were mainly detected in basic extracts. There were in total seven chitinase isoforms of three basic and four acidic isoforms in Rehmannia. The range of molecular weight of Rehmannia chitinase was from 28 to 32 kD. It showed the possibility of tissue specific expression of acidic chitinases and basic chitinases were mainly detected in root.

• Food Science and Preservation
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• v.11 no.2
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• pp.282-285
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• 2004

This experiment was carried out to investigate the cost reduction of rhizome through the stable storage of Jiwhang #1(Rehmannia glutinosa Libosch). The growth characteristics of Jiwhang #1 are different from those of local Rehmannia glutinosa L. varieties in Korea. Various storage methods was introduced and analyzed for 120 days. Among storage methods, changes in mean daily temperature in storage location were the least from 7.2 to 13.8$^{\circ}C$ in underground storage, while those were wide in styrofoam box filled with soil, and changes in mean daily relative humidity were the highest 98.2% in underground storage. As the storage period increased, the rhizome brix and the rate of rhizome loss and spoiled rhizome increased. The brix and loss rate of rhizome were higher in net sack filled with hull and styrofoam box filled with hull, but the rate of spoiled rhizome was the greatest in underground storage among storage methods. As the storage period increased, the hardness of rhizome epidermis increased, while that of rhizome endodermis decreased.