• Applied Biological Chemistry
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• 제32권3호
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• pp.240-248
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• 1989

한방(韓方)의 요약(要藥)인 지황(地黃)의 품질향상(品質向上)을 위한 배재지선정(培栽地選定)의 기초자료(基礎資料)를 얻기 위하여 재배지토양(栽培地土壤)의 이화학성(理化學性)이 지황(地黃)의 품질(品質)을 좌우하는 catalpol, 당류(糖類), 회분(灰分) 및 엑기스들의 함량(含量)에 미치는 영향(影響)을 조사(調査)하였다. 재배지(栽培地)의 토성(土性)은 사질식양토(砂質植壤土) 및 사양토(砂壤土)가 많았다. Catalpol 함량(含量)은 $3.75{\sim}4.36%$의 범위(範圍)에 속(屬)하였다. 표토(表土) 및 심토(心土)의 모래함량(含量)은 catalpol 함량(含量)과는 정(正)의 상관(相關)이, fructose 및 galactose의 함량(含量)과는 부(負)의 상관(相關)이 인정(認定)되었으며 심토(心土)의 모래함량(含量)은 회분(灰分) 및 산불용성회분(酸不溶性灰分)의 함량(含量)과 부(負)의 상관(相關)이 인정(認定)되었다. 심토(心土)의 점토함량(粘土含量)은 catalpol 함량(含量)과 부(負)의 상관(相關)이, fructose, galactose 및 sucrose들의 함량(含量)과는 정(正)의 상관(相關)이 인정(認定)되었다. 표토(表土) 및 심토(心土)의 점토함량(粘土含量)은 회분함량(灰分含量)과 정(正)의 상관(相關)이, 엑기스들의 함량(含量)과는 부(負)의 상관(相關)이 인정(認定)되었다. 토양중(土壤中) 유기함물량(有機含物量)은 catalpol 및 엑기스들의 함량(含量)과 부(負)의 상관(相關)이, fructose 및 회분함량(灰分含量)과는 정(正)의 상관(相關)이 인정(認定)되었다. 토양중(土壤中) 유기함물량(有機含物量)은 catalpol 및 엑기스들의 함량(含量)과 부(負)의 상관(相關)이, fructose 및 회분함량(灰分含量)과는 정(正)의 상관(相關)이 인정(認定)되었다. 토양중(土壤中) 전질소함량(全窒素含量)은 catalpol 함량(含量)과 부(負)의 상관(相關)이, fructose 및 sucrose의 함량(含量)과는 정(正)의 상관(相關)이 인정(認定)되었다. 토양중(土壤中) 가리함량(加里含量)은 fructose의 함량(含量)과 고도(高度)의 정(正)의 상관(相關)이 인정(認定)되었다.

• 동의생리병리학회지
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• 제23권1호
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• pp.76-83
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• 2009

Rehmannia Radix Preparata (RRP) used to nourish Eum and enrich blood for consumptive fever, aching, and limpness of the loins and knees, and to replenish essence for tinnitus, premature greying of beard and hair. In the present study, we studied about the protective effect of RRP on hydrogen peroxide-induced oxidative stress in human vascular endothelial cells. ECV304 cells were preincubated with RRP (100, 200, 300 and $400{\mu}g/m{\ell}$) for 12hr and then treated with $600{\mu}M$ $H_2O_2$ for 12hr. The protective effects of RRP on $H_2O_2$-induced apoptosis in ECV304 cells was determined by using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. The results of this experiment showed that RRP inhibited $H_2O_2$-induced apoptosis and ROS production in ECV304 cells. Moreover, RRP increased ERK activation that decreased in $H_2O_2$-treated ECV304 cells, and inhibited p38 and JNK activation. Furthermore, RRP increased expression of heme oxygenase-1 (HO-1) in $H_2O_2$-treated ECV304 cells. Also, HO-1 protein expression induced by RRP was reduced by the addition of ERK inhibitor (PD98059) in $H_2O_2$-treated ECV304 cells. These results suggest that protective effect of RRP on $H_2O_2$-induced oxidative stress in ECV304 cells may be associated with increase of ERK activation and HO-1 protein, and reduction of p38 and JNK activation.

• KSBB Journal
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• 제16권5호
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• pp.486-492
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• 2001

숙지황 물추출물로부터 분리된 fraction(RG-III) 의 항돌연변이원성의 기작을 E. coli GW, B/r 균주를 이용하여 조사하였다. SOS 유도를 반영하는 $\beta$-galactiosidase 활성이 E. coli GW 1060, 1103, 1107, 1105에서 증가되지 않았다. RG-III는 RecA는 단백질의 합성을 증폭시키거나 LexA 산물의 분해를 저해하지 않았으므로 SOS en 기능의 발현이 영향을 미치지 못했다. 따라서 DNA 수복의 경로가 다른 E. coli B.r 변이주를 사용하여 4NQO와 MNNG에 대한 세포내 항돌연변이원성과 생존효과를 조사하였다. ZA159(uvrB, 최)를 제외한 WP2, WP2s, WP67, CM561, CM611에서 RG-III는 4NQO에 대한 생존력을 미약하게나마 증가시켰으나, 이러한 생존력 재활성을 수복모드에 의해 설명할 수 없었다. WP2, WP2s, WP67, CM561, CM611에서 RG-III는 MNNG로 유도된 돌연변이원성과 치사력을 증가시킴에도 불구하고 ZA159(uvr B, chl)에서는 감소시켰다. 4NQO의 변이원성을 두드러지게 억제하였으나 ZA159(uvr B, chl)에서 상승효과가 상대적으로 감소되었다. 이러한 결과들은 RG-III가 4NQO의 변이원성을 방어하는 차단제임을 시시하여, chl 산물의 기능과 유사한 작용을 하는 것으로 사료된다.

• 한국약용작물학회지
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• 제8권3호
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• pp.266-273
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• 2000

지황 신품종(新品種) 육성(育成)의 기초 자료를 마련하고 자 RAPD 기법을 이용하여 국내외(國內外)에서 수집(蒐集)된 지황 12계통의 계통간(系統間) 유연관계(類緣關係)를 분석하여 다음과 같은 결과를 얻었다. PCR 증폭의 최적 조건은 총(總) 반응용액(反應溶液) $20\;{\mu}l$ 중 template DNA 20 ng, dNTP 250 mM, primer 10 pM, Taq DNA polymerase 1.0 unit, annealing 온도는 $36^{\circ}C$이었다. 20가지의 OPB random primer를 PCR한 결과(結果) 17개의 primer를 선발(選拔)하였으며 다형을 보이는 band수는 107개였다. 선정된 17개의 primer에의해 증폭된 DNA 단편(斷片)들은 $400{\sim}3,200\;bp$ 범위에 속하였으며, primer 당 나타난 band 수는 $1{\sim}10$개 까지였고 평균 6.1개 이었다. Jaccard 와 Nei 상관계수에 따라 지황의 12계통은 가운데에서 나타난 군(群)은 크게 3개군(群)으로 구분되었는 데, 우리나라 지황 수집(蒐集) 계통(系統) 중 정읍, 서천, 진안, 안동 및 단양 재래계통은 계통(系統)간 유연관계(類緣關係)가 $0.27{\sim}0.05$로 가깝게 나타났다. (제1군(群)). 수원 조직배양(組織培養) 계통(系統)과 춘천 재래(在來) 계통(系統)은 $0.29{\sim}0.11$로 유연관계가 가깝게 나타났으며, $F_1$ ( 지황 1 호${\times}$ 서천 재래계통 )과 단양 조직배양(組織培養) 계통(系統), 일본계량(日本改良) 계통도 이들과 계통간(系統間) 유연관계(類緣關係)가 가까운 군(群)에 속(屬)하였다 (제 2군(群)). 지황1호와 일본(日本) 재래(在來) 계통(系統)의 유연관계 는 각각 0.35 와 0.43 으로 우리나라 재래종(在來種)과 원연관계(遠緣關係)인 것으로 밝혀졌다.

• 대한본초학회지
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• 제27권6호
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• pp.49-54
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• 2012

Objective : We recently reported that OMC-2010 has an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-5. However, we did not find out which constituents play an important role in immuno-modulatory effect of OMC-2010. Thus, this study was performed to estimate the effects of constituents of OMC-2010 on cytokine production in mouse spleen cells, then ultimately reach to find out effective constituents regulating splenic cytokine production. Methods : Mouse spleen cells were pre-treated with water and ethanol extract of constituents of OMC-2010 such as Rehmannia glutinosa (RG), Pinellia ternata (PT), Citrus unshiu Markovich (CUM), Glycyrrhiza uralensis (GU), Platycodon grandiflorum (PG), Schisandra chinensis (SC). After 1 h, the cells were stimulated with lipopolysaccharide (LPS, 1 ${\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions. Results : The water extract of RG extract significantly inhibited the LPS-induced inTNF-${\alpha}$ and IL-5 mRNA expressions, but the water extract of PT, CUM, GU, PG, and SC did not. The ethanol extract of RG, PT, and SC significantly inhibited the LPS-induced TNF-${\alpha}$, and IL-5 mRNA expressions, but the ethanol extract of CUM, GU, and PG did not. Conclusions : Theses results could suggest that the water extract of RG and the ethanol extract of RG, PT, and SC inhibited the expression of TNF-${\alpha}$ and IL-5, which means that the possible candidate of OMC-2010 water extract's action might be RG, and ethanol extract's action might be RG, PR, and SC.

• 사상체질의학회지
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• 제34권4호
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• pp.1-12
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• 2022

Objectives This paper investigates the origins, changes, and the constructive principles of Hyungbangjihwang-tang(荊防地黃湯). Methods Hyungbangjihwang-tang and other related prescriptions were analyzed in terms of their pathological indications, based on previous literature including 『Gogeumuigam(古今醫鑑)』, 『Sanghannon(傷寒論)』, 『Uihagjeongjeon(醫學正傳)』, 『Donguisusebowon·Sasangchobongwon(東醫壽世保元·四象草本卷)』, 『Donguisusebowon·Gabobon(東醫壽世保元·甲午本)』, 『Donguisusebowon·Sinchukbon(東醫壽世保元·辛丑本)』. Results and Conclusions The origins of Hyungbangjihwang-tang can be seen in three sections depending on the classification of medicinal herbs. Schizonepeta tenuifolia Briq.(荊芥), Saposhnikovia divaricata Schischk.(防風), Notopterygium incisum Ting(羌活), Aralia cordata Thunb.(獨活), and Plantago asiatica L.(車前子) originated from Hyungbangpaedok-san(荊防敗毒散) included in 『Gogeumuigam』. Poria cocos Wolf.(茯苓) and Alisma canaliculatum(澤瀉) originated from Ohryoung-san(五苓散) included in 『Sanghannon』. Rehmannia glutinosa for hueichingensis(熟地黃) and Cornus officinalis S. et Z.(⼭茱萸) originated from Yukmijihwang-tang(六味地黃湯) included in 『Uihagjeongjeon』. Hyungbangpaedok-san was transformed into "Paedok-san" in 『Chobongwon(草本卷)』, Hyungbangpaedok-san in the 『Gabobon(甲午本)』 and 『Sinchukbon(辛丑本)』. Yukmijihwang-tang was mentioned as a Soyangin medicine in the 『Chobongwon』 and was later transformed into a Suhwagije-tang(水火旣濟湯) in 『Gabobon』. The meaning of Yukmijihwang-tang inherited in 『Sinchukbon』 as Dokhwaljihwang-tang and Hyungbangjihwang-tang. The medicinal herbs in Hyungbangjihwang-tang can be categorized in three sections by their functions, Schizonepeta tenuifolia Briq., Saposhnikovia divaricata Schischk., Notopterygium incisum Ting, Aralia cordata Thunb., and Plantago asiatica L. scatter the Pyohan(表寒) and make the Pyoeum(表陰) drop. Poria cocos Wolf.(茯苓) and Alisma canaliculatum(澤瀉) make the Pyoeum(表陰) drop through Lee-su(利水). Rehmannia glutinosa for hueichingensis(熟地黃) and Cornus officinalis S. et Z.(⼭茱萸) tonify Shin-won(腎元). The each principles of medicinal herbs corresponds to the stage of the "Cold-related Diarrhea in Soyangin Spleen Cold-based Exterior Cold disease(少陽人 脾受寒表寒病 亡陰病 身寒腹痛亡陰證)".

• 동의생리병리학회지
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• 제25권6호
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• pp.1056-1060
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• 2011

To summarize and make a reference number of natural materials used to treat diabetic peripheral neuropathy. We surveyed all papers of diabetic peripheral neuropathy studies using natural materials in PubMed as "diabetic peripheral neuropathy AND plant", "diabetic peripheral neuropathy AND herbal", "diabetic peripheral neuropathy AND herb", etc. The number of papers, the formation of experiments, frequency of natural materials studies, and main studies were analyzed. Total 48 studies were finally selected. Of the papers, experiments with rats were the most common. Most studies were about fatty acids or herbal medicines. Rehmannia glutinosa, Cinnamomi Ramulus, Astragali Radix and so on were relatively studied much. This study produced an overview of worldwide natural materials used for diabetic peripheral neuropathy. This result may provide a valuable information of development of Korean herbal medicine used to treat diabetic peripheral neuropathy.

• 한국토양비료학회지
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• 제47권6호
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• pp.506-509
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• 2014

This study was conducted to identify transition characteristics of mercury in several selected medicinal plants and to find the appropriate management for production of safety food. Cultivated soils and medicinal plants were collected at 29 sites for Angelica gigas (Korean angelica root), 68 sites for Platycodon grandiflorum (Balloon flower), 35 sites for codonopsis lanceolata (Deoduck), 36 sites for Dioscorea batatas (Chinese yam), 32 sites for Rehmannia glutinosa (Foxglove), 16 sites for Cnidium officinale makino (cnidium), and 26 sites for Astragalus membranaceus (milk vetch root) during the harvest season of 2013. Mercury in the soils and medicinal roots were analyzed with a Direct Mercury Analyzer. Average content of mercury in soils cultivated medicinal plants was $0.023mg\;kg^{-1}$ (range: from 0.003 to $0.074mg\;kg^{-1}$) and average content of mercury in medicinal plants was $0.003mg\;kg^{-1}$ (range: from 0.001 to $0.011mg\;kg^{-1}$), indicating that mercury in the surveyed soils and medicinal plants were not exceeded the Korean regulation.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.214-215
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• 2011

• Advances in Traditional Medicine
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• 제4권3호
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• pp.125-136
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• 2004

The pharmacological mechanisms of most Oriental medicines have not been clearly defined in spite of their effective use in treating many diseases throughout the world. Many Oriental medicines have been used against various allergic diseases for generations, and still occupy an important place in traditional medicine in far eastern countries including Korea. It is also still unclear how Oriental drugs prevent allergic disease in vivo or in vitro models. Some Korean folk medicine inhibited the mast cell-mediated allergic reaction. This review summarizes the effective folk medicine in experimental effect on systemic or local anaphylaxis reaction. Potential anti-anaphylactic folk medicines include: Poncirus trifoliata; Siegesbeckia glabrescence; Solanum lyratum; Aquilaria agallocha; Ulmi radicis; Polygonum tinctorium; Hwanglyun-Haedok-Tang; Rehmannia glutinosa; Kum-Hwag-San; Syzygium aromaticm; Spirulina platensis; Sosiho-Tang; Sinomenium acutum; Schizonepta tenuifolia; Shini-San; Magnoliae flos; Sochungryong-Tang; Oryza sativa; Cryptotympana atrata; Salviae radix; Rosa davurica; Asiasari radix; Chung-Dae-San; Cichorium intybus; Perilla frutescens; Vitex rotundifolia; Terminalia chebula; Siberian Ginseng; Solanum melongena; Gahmi-Shini-San; Alpinia oxyphylla; Acanthopanax senticosus root; Prunella vulgaris; Allergina; Ixeris dentate; Acanthopanax senticosus stem; Tongkyutang; Salvia plebeia; Rubus coreanus; Sinpo- Tang; Dodutang; Forsythia fructus; Xanthii fructus; and Purple bamboo slat. Ensuring the effects and understanding the mechanisms of action for these Oriental medicines can permit drug development and laying of the ground-work for evaluating potential synergistic effects by addition and subtraction of prescriptions.