Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Rehmanniae Radix Preparata Extract on $H_2O_2$-induced Apoptosis of ECV304 Cells

숙지황(熟地黃) 추출물이 $H_2O_2$에 의해 유도된 ECV304 세포의 apoptosis에 미치는 영향

  • Kim, In-Gyu (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Jin-Mo (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Jae (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yang, Hyun-Mo (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Won-Sin (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
  • 김인규 (원광대학교 한의과대학 병리학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 박진모 (원광대학교 한의과대학 병리학교실) ;
  • 전병제 (원광대학교 한의과대학 병리학교실) ;
  • 양현모 (원광대학교 한의과대학 병리학교실) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2009.02.25
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Abstract

Rehmannia Radix Preparata (RRP) used to nourish Eum and enrich blood for consumptive fever, aching, and limpness of the loins and knees, and to replenish essence for tinnitus, premature greying of beard and hair. In the present study, we studied about the protective effect of RRP on hydrogen peroxide-induced oxidative stress in human vascular endothelial cells. ECV304 cells were preincubated with RRP (100, 200, 300 and $400{\mu}g/m{\ell}$) for 12hr and then treated with $600{\mu}M$ $H_2O_2$ for 12hr. The protective effects of RRP on $H_2O_2$-induced apoptosis in ECV304 cells was determined by using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. The results of this experiment showed that RRP inhibited $H_2O_2$-induced apoptosis and ROS production in ECV304 cells. Moreover, RRP increased ERK activation that decreased in $H_2O_2$-treated ECV304 cells, and inhibited p38 and JNK activation. Furthermore, RRP increased expression of heme oxygenase-1 (HO-1) in $H_2O_2$-treated ECV304 cells. Also, HO-1 protein expression induced by RRP was reduced by the addition of ERK inhibitor (PD98059) in $H_2O_2$-treated ECV304 cells. These results suggest that protective effect of RRP on $H_2O_2$-induced oxidative stress in ECV304 cells may be associated with increase of ERK activation and HO-1 protein, and reduction of p38 and JNK activation.

Keywords

References

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