• Journal of Plant Biotechnology
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• v.41 no.1
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• pp.38-43
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• 2014

To establish the system of in vitro plant regeneration, the different explants (stem with axillary bud and stem without axillary bud) of Hylotelephium ussuriense were cultured on the Murashige and Skoog's medium containing 6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in the stem with axillary bud explants than the stem without axillary bud explants, and was the best on MS medium containing 3.0 mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growth was not significantly treated on MS and sucrose. Total chlorophyll contents under ventilation treatment were higher than those in control (non-ventilation). This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.711-719
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• 2018

BACKGROUND: Collagen organization within tissues has a critical role in wound regeneration. Collagen fibril diameter, arrangements and maturity between connective tissue growth factor (CTGF) small interfering RNA (siRNA) and mismatch scrambled siRNA-treated wound were compared to evaluate the efficacy of CTGF siRNA as a future implement for scar preventive medicine. METHODS: Nanocomplexes of CTGF small interfering RNA (CTGF siRNA) with cell penetrating peptides (KALA and $MPG^{{\Delta}NLS}$) were formulated and their effects on CTGF downregulation, collagen fibril diameter and arrangement were investigated. Various ratios of CTGF siRNA and peptide complexes were prepared and down-regulation were evaluated by immunoblot analysis. Control and CTGF siRNA modified cells-populated collagen lattices were prepared and rates of contraction measured. Collagen organization in rabbit ear 8 mm biopsy punch wound at 1 day to 8 wks post injury time were investigated by transmission electron microscopy and histology was investigated with Olympus System and TS-Auto software. CONCLUSION: CTGF expression was down-regulated to 40% of control by CTGF siRNA/KALA (1:24) complexes (p<0.01) and collagen lattice contraction was inhibited. However, down-regulated of CTGF by CTGF $siRNA/MPG^{{\Delta}NLS}$ complexes was not statistically significant. CTGF KALA-treated wound appeared with well formed-basket weave pattern of collagen fibrils with mean diameter of $128{\pm}22nm$ (n = 821). Mismatch siRNA/KALA-treated wound showed a high frequency of parallel small diameter fibrils (mean $90{\pm}20nm$, n = 563). CONCLUSION: Controlling over-expression of CTGF by peptide-mediated siRNA delivery could improve the collagen orientation and tissue remodeling in full thickness rabbit ear wound.

• Journal of Forest and Environmental Science
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• v.37 no.4
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• pp.304-308
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• 2021

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

• Journal of Creative Information Culture
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• v.6 no.3
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• pp.209-217
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• 2020

Recently, interest in data analysis has increased, and convergence research through data analysis has been actively conducted in various fields such as engineering, natural science, and social science. In the field of architecture, various studies using data analysis are being conducted, and in particular, efforts are being made to solve the problems in the field of architecture that have been quantitatively expanded through the urbanization process. In this study, data analysis on residential satisfaction of residents in residential environment improvement areas and similar neighborhoods through urban regeneration projects is performed. Through analysis using R for post-residential evaluation elements that are conducted after building construction and occupancy, important evaluation items that affect the satisfaction of the residential environment are identified by analyzing the association rules between each evaluation element and identifying the frequency of major requirements of residents. To grasp. Through this, we intend to conduct convergence research between IT and architecture fields, such as the development of a system that can recommend high-quality residential areas as well as providing data for securing high-quality residential spaces when constructing residential areas in the future.

• BMB Reports
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• v.55 no.5
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• pp.205-212
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• 2022

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating chronic disorder characterized by suprapubic pain and urinary symptoms such as urgency, nocturia, and frequency. The prevalence of IC/BPS is increasing as diagnostic criteria become more comprehensive. Conventional pharmacotherapy against IC/BPS has shown suboptimal effects, and consequently, patients with end-stage IC/BPS are subjected to surgery. The novel treatment strategies should have two main functions, anti-inflammatory action and the regeneration of glycosaminoglycan and urothelium layers. Stem cell therapy has been shown to have dual functions. Mesenchymal stem cells (MSCs) are a promising therapeutic option for IC/BPS, but they come with several shortcomings, such as immune activation and tumorigenicity. MSC-derived extracellular vesicles (MSC-EVs) hold numerous therapeutic cargos and are thus a viable cell-free therapeutic option. In this review, we provide a brief overview of IC/BPS pathophysiology and limitations of the MSC-based therapies. Then we provide a detailed explanation and discussion of therapeutic applications of EVs in IC/BPS as well as the possible mechanisms. We believe our review will give an insight into the strengths and drawbacks of EV-mediated IC/BPS therapy and will provide a basis for further development.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.303-308
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• 1994

Shoot apical meristem dome explants from cassava plants (Ghanaian local variety) produced somatic embryos at a frequency of 32% when cultured on MS medium supplemented with 2 mg/L 2,4-D. Somatic embryo segments formed secondary embryos at frequencies of up to 93% when cultured on medium containing 1 mg/L 2,4-D for 2 to 3 weeks. Since the somatic embryos were not capable of converting into plantlets, adventitious shoot were induced from the sliced embryo segments by culturing them on medium containing 0.1 to 5 mg/L BA. After 8 weeks of culture, numerous shoots formed on the segments at frequencies up to 100%. The shoots were rooted and successfully transplanted to potting soil.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.1-6
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• 2000

This study was carried out to find the effects of plant growth regulators on callus formation, rooting and shooting from cotyledon explant in oriental me]on. Various combinations of 0.1 mg/L auxins (IAA, NAA) and 0.5, 1.0. 1.5, 2.0 mg/L cytokinins (BA, kinetin, zeatin) were treated to the MS basal medium, respectively. Callus was induced mort effectively as 2,437.0 mg (FW)/explant in MS medium supplemented with 0.1 mg/L NAA and 2.0 mg/L BA, but that was non-embryogenic callus as colored yellow white and broke easily. Root was induced most effectively at a frequency of 98.0% in MS medium supplemented with 0.1 mg/L NAA and 0-5 mg/L kinetin. Shoots formed on cut part of vein at a frequency of 98.0% in MS medium supplemented with 0.1 mg/L IAA and 2.0 mg/L BA, that were multiple shoots. in case of its concentration, BA and lower concentration of IAA and NAA (0.01 and 0.05 mg/L). respectively. shooting ratio was not increased. The result of treatment with BA 0-5 mg/L and IAA 0.1 mg/L, callus induced at a week, and shoot start to form multiple shoots about 3 weeks after inoculation. After 2 times subculture as 2 weeks intervals, divided shoots rooted and developed into intact plantlets at 10 weeks and then that grown normally on pots after acclimatization.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.251-257
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• 1999

Adventitious buds were produced from the cultures of mature zygotic embryos of Larix leptolepis with the highest frequency of 91.7% in SH medium containing 1.0 mg/L zeatin. The most effective cytokinin combination for inducing adventitious buds was 1.0 mg/L zeatin + 1.0 mg/L thidiazuron (40.3%). The highest mean number of adventitious buds induced in 1.0 mg/L zeatin + 1.0 mg/L 2iP or 1.0 mg/L zeatin + 1.0 mg/L kinetin combined treatments. Elongation of the adventitious buds occurred the best on half strength LM salts medium, on which the buds elongated upto 27 mm. Also, the supplement of activated charcoal in medium suppressed the elongation of the adventitious buds. The highest frequency (23.3%) of rooting from elongated shoots was obtained on the medium containing 5.0 mg/L IBA and 0.2 mg/L NAA. The highest number (3.5) of roots was induced on the medium containing 5.0 mg/L IBA alone.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.259-264
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• 2002

Induction of embryogenic callus from Allium wakegi Araki explants was promoted on medium containing 2,4-D, and production of abnormal embryos from the embryogenic callus increased as 2,4-D concentration was raised. Shoot tip was found to be the best explant source for embryogenic callus formation followed in the order by bulb scale and leaf section. Medium containing 0.09 M sucrose was effective for embryogenic callus production. The regenerants from embryogenic callus on medium containing 2,4-D and BA at different concentrations was consisted of diploids, tetraploids and a few mixaploids of 2n＋4n, and their chromosomal aberration rate ranged from 8.0 to 33.3%. Frequency of chromosomal aberrants was the highest (18.7%) in the regenerants obtained from bulb scale-derived embryogenic callus among others. Plant regeneration rate was high (33.5%) from the shoot tip-derived embryogenic callus and the frequency of chromosomal aberrants was very low (7.0%). The plantlets regenerated on medium containing 0.26 M sucrose resulted in high chromosomal aberrants. The regenerants on medium containing sucrose at 0.09∼0.20 M produced chromosomal aberrants at around 15.2∼16.6%.

• Journal of Korean Society of Forest Science
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• v.111 no.1
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• pp.61-71
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• 2022

Data from an investigation of vulnerable conifer species in the subalpine zone in Korea obtained by the Korea Forest Service in 2017-2018 and monitoring research conducted by the National Institute of Forest Science since 2014 were used to analyze the status of distribution and growing condition of three major conifer species (Abies nephrolepis, Abies koreana, and Picea jezoensis) in the subalpine zone in the Baekdudaegan protected area. The distribution area of the studied species in the Baekdudaegan protected area was ca. 74% (8,035 ha) of the total distribution area in Korea, indicating that Baekdudaegan is a core area for conservation and restoration of subalpine conifer species. From decline index [A. nephrolepis in Mt. Taebaeksan and Mt. Deogyusan increased by 77.3% and 29.6%, respectively; A. koreana in Mt. Jirisan (Chunwangbong Peak) increased by 45.2% in four years; and P. jezoensis in Mt. Jirisan (Chunwangbong Peak) increased by 47.8% in two years] and seedling frequency (lower frequency of newly recruited seedlings than dead seedlings) results, the studied species are expected to face difficulties in sustainability. In contrast, at Mt. Seseoksan and Chunwangbong Peak in Mt. Jirisan, the health of trees and seedling frequency showed a partial tendency to recover and increase. In addition, we identified the relationship between the decline index and seedling frequency. These results will support the implementation of conservation strategies for vulnerable conifer species in the subalpine zone.