Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Plant Regeneration of Iris koreana Nakai through Organogenesis for Ex-situ Conservation

  • Bae, Kee-Hwa (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Yun, I-Seul (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Jung, Ji-Sun (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Kim, Chan-Beom (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Kim, Hye-Won (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Hong, Yong-Sik (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Oak, Min-Kyeong (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Kim, Hak-Koo (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum) ;
  • Lee, Ju-Hui (Department of Temperate Middle Part Plant Conservation, Sejong National Arboretum)
  • Received : 2021.11.05
  • Accepted : 2021.12.05
  • Published : 2021.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.

Keywords

Acknowledgement

This work was supported by a research grant of the Sejong National Arboretum (SNA) in 2021.

References

  1. Ayabe M, Sumi S. 1998. Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Rep 17: 773-779. https://doi.org/10.1007/s002990050481
  2. Ayabe M, Taniguchi K, Sumi S. 1995. Regeneration of whole plants from protoplasts isolated from tissue-cultured shoot primordia of garlic (Allium sativum L.). Plant Cell Rep 15: 17-21. https://doi.org/10.1007/BF01690245
  3. Baruch ER, Quak F. 1966. Virus-free plants of iris 'wedgwood' obtained by meristem culture. Neth J Plant Pathol 72: 270-273. https://doi.org/10.1007/BF02650217
  4. Boltenkov EV, Rybin VG, Zarembo EV. 2004. [Cultivation of Iris ensata Thunb. callus tissue]. Prikl Biokhim Mikrobiol 40: 244-251.
  5. Geier T. 1990. Anthurium. In: Handbook of Plant Cell Culture. Volume 5 Ornamental species (Ammarito PV, Evans DA, Sharp WR, Bajaj YPS, eds). MacMillan Publishing Company, New York, pp 228-252.
  6. Gozu Y, Yokoyama M, Nakamura M, Namba R, Yomogida K, Yanagi M, Nakamura S. 1993. In vitro propagation of Iris pallida. Plant Cell Rep 13: 12-16. https://doi.org/10.1007/BF00232307
  7. Guo DP, Zhu ZJ, Hu XX, Zheng SJ. 2005. Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai). Plant Cell Tissue Organ Cult 83: 123-127. https://doi.org/10.1007/s11240-005-3799-5
  8. Hussey G. 1975. Totipotency in Tissue Explants and Callus of Some Members of the Liliaceae, Iridaceae, and Amaryllidaceae. J Exp Bot 26: 253-262. https://doi.org/10.1093/jxb/26.2.253
  9. Ichihashi S, Kato S. 1986. Clonal propagation of Iris kaempferi by means of flower organ culture. Bull Aichi Univ Edu 35: 135-143.
  10. Jehan H, Courtois D, Ehret C, Lerch K, Petiard V. 1994. Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apices and young flowers. Plant Cell Rep 13: 671-675. https://doi.org/10.1007/BF00231621
  11. Kawase K, Mizutani H, Yoshioka M, Fukuda S. 1991. Propagation of Iris by tissue culture. 2. Culture of flower organs. J Japan Soc Hort Sci 60: 436-437.
  12. Kawase K, Mizutani H, Yoshioka M, Fukuda S. 1995. Shoot Formation on Floral Organs of Japanese Iris in Vitro. J Japan Soc Hort Sci 64: 143-148. https://doi.org/10.2503/jjshs.64.143
  13. Laublin G, Cappadocia M. 1992. In Vitro Ovary Culture of some Apogon Garden Irises (Iris pseudacorus L., I. setosa Pall., I. versicolor L.). Bot Acta 105: 319-322. https://doi.org/10.1111/j.1438-8677.1992.tb00305.x
  14. Laublin G, Saini HS, Cappadocia M. 1991. In vitro plant regeneration via somatic embryogenesis from root culture of some rhizomatous irises. Plant Cell Tissue Organ Cult 27: 15-21. https://doi.org/10.1007/BF00048200
  15. Lee JS, Choi BH. 2006. Distributions and Red Data of Wild Orchids in the Korean Peninsula. Korean J Plant Taxon 36: 335-360. https://doi.org/10.11110/kjpt.2006.36.4.335
  16. Liu H, Fan L, Song H, Tang B, Wang L. 2020. Establishment of regeneration system of callus pathway for Iris sanguinea Donn ex Horn. In Vitro Cell Dev Biol Plant 56: 694-702. https://doi.org/10.1007/s11627-020-10124-6
  17. Luciani GF, Mary AK, Pellegrini C, Curvetto NR. 2006. Effects of explants and growth regulators in garlic callus formation and plant regeneration. Plant Cell Tissue Organ Cult 87: 139-143. https://doi.org/10.1007/s11240-006-9148-5
  18. Mielke K, Anderson WC. 1989. In vitro bulblet formation in Dutch iris. HortScience 24: 1028-1031. https://doi.org/10.21273/HORTSCI.24.6.1028
  19. Murashige T, Skoog F. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Myers JM, Simon PW. 1999. Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time. Plant Cell Rep 19: 32-36. https://doi.org/10.1007/s002990050706
  21. Radojevic L, Sokic O, Tucic B. 1987. Somatic embryogenesis in tissue culture of iris (iris pumila L.). Acta Hortic 212: 719-723. https://doi.org/10.17660/actahortic.1987.212.124
  22. Radojevic L, Subotic A. 1992. Plant Regeneration of Iris setosa Pall. Through Somatic Embryogenesis and Organogenesis. J Plant Physiol 139: 690-696. https://doi.org/10.1016/s0176-1617(11)81712-8
  23. SAS. 2001. SAS/STAT User's Guide, Version 8.02. SAS Institute, Cary, NC.
  24. Shibli RA, Ajlouni MM. 2000. Somatic embryogenesis in the endemic black iris. Plant Cell Tissue Organ Cult 61: 15-21. https://doi.org/10.1023/A:1006468122819
  25. Shimizu K, Nagaike H, Yabuya T, Adachi T. 1997. Plant regeneration from suspension culture of Iris germanica 1. Plant Cell Tissue Organ Cult 50: 27-31. https://doi.org/10.1023/A:1005910710066
  26. Shimizu K, Yabuya T, Adachi T. 1996. Plant regeneration from protoplasts of Iris germanica L. Euphytica 89: 223-227. https://doi.org/10.1007/BF00034609
  27. Wang WC, Nguyen HT. 1990. A novel approach for efficient plant regeneration from long-term suspension culture of wheat. Plant Cell Rep 8: 639-642. https://doi.org/10.1007/BF00269982
  28. Wang Y, Jeknic Z, Ernst RC, Chen THH. 1999a. Efficient Plant Regeneration from Suspension-cultured Cells of Tall Bearded Iris. HortScience 34: 730-735. https://doi.org/10.21273/HORTSCI.34.4.730
  29. Wang Y, Jeknic Z, Ernst RC, Chen THH. 1999b. Improved Plant Regeneration from Suspension-cultured Cells of Iris germanica L. 'Skating Party'. HortScience 34: 1271-1276. https://doi.org/10.21273/hortsci.34.7.1271
  30. Xu Z, Um YC, Kim CH, Lu G, Guo DP, Liu HL, Bah AA, Mao A. 2008. Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation. Acta Physiol Plant 30: 521-528. https://doi.org/10.1007/s11738-008-0150-x
  31. Yabuya T, Ikeda Y, Adachi T. 1991. In vitro propagation of Japanese garden iris, Iris ensata Thunb. Euphytica 57: 77-81. https://doi.org/10.1007/BF00040480
  32. Zheng S, Henken B, Sofiari E, Jacobsen E, Krens FA, Kik C. 1998. Factors influencing induction, propagation and regeneration of mature zygotic embryo-derived callus from Allium cepa. Plant Cell Tissue Organ Cult 53: 99-105. https://doi.org/10.1023/A:1006034623942