• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.321-331
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• 2011

Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.

• The Plant Pathology Journal
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• v.21 no.2
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• pp.158-163
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• 2005

Virus-induced gene silencing (VIGS) is a recently developed gene transcript suppression technique for characterizing the function of plant genes. However, efficient VIGS has only been studied in a few plant species. In order to extend the application of VIGS, we examined whether a VIGS vector based on TRV would produce recognizable phenotypes in soybean. Here, we report that VIGS using the Tobacco rattle virus (TRV) viral vector can be used in several soybean cultivars employing various agro-inoculation methods including leaf infiltration, spray inoculation, and agrodrench. cDNA fragments of the soybean phytoene desaturase(PDS) was inserted into TRV RNA-2 vector. By agrodrench, we successfully silenced the expression of PDS encoding gene in soybean. The silenced phenotype of PDS was invariably obvious 3 weeks after inoculation with the TRV-based vector. Real-time RT-PCR analyses showed that the endogenous level of GmPDS transcripts was dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. The TRV-based VIGS using agrodrench can be applied to functional genomics in a soybean plants to study genes involved in a wide range of biological processes. To our knowledge, this is the first high frequency VIGS method in soybean plants.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.521-529
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• 2019

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

• Korean Journal of Computational Design and Engineering
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• v.18 no.1
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• pp.21-27
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• 2013

Presented in the paper is a log data based modeling method for effective construction of a virtual plant model which can be used for the virtual PLC (Programmable Logic Controller) simulation. For the PLC simulation, the corresponding virtual plant, consisting of virtual devices, is required to interact with the input and output symbols of a PLC. In other words, the behavior of a virtual device should be the same as that of the real device. Conventionally, the DEVS (Discrete Event Systems Specifications) formalism has been used to represent the behavior a virtual device. The modeling using DEVS formalism, however, requires in-depth knowledge in the simulation area, as well as the significant amount of time and efforts. One of the key ideas of the proposed method is to generate a plant model based on the log data obtained from the production system. The proposed method is very intuitive, and it can be used to generate the full behavior model of a virtual device. The proposed approach was applied to an AGV (Automated Guided Vehicle).

• Proceedings of the KIEE Conference
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• 2004.11c
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• pp.535-537
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• 2004

The flame image processing and it's analysis system has been developed for optimal coal firing of thermal power plant, especially for reducing NOx and safe operations. We aimed at gaining the relationship between burner flame image, emissions of NOx and LOI(Loss on ignition) in furnace by utilizing the flame image processing methods. And the relationship determines quantitatively the conditions of combustion on the individual burners. The test was conducted on Samchonpo thermal power plant #4 unit(560MW) of KOSEP which has 24 burners. The system simplified the burner adjustment works in accordance with the real time trending of flame behavior like NOx profiles and unburned carbon profiles for individual burners. But, This kind of conventional method increases the cost as the number of burner are increased. Also there is a difficulty to measure exhausted gas of each burner because of measurement errors. This paper intends to propose the useful "Flame Monitoring System" that can find Low NOX and LOI at the upper furnace and to compare with the conventional System.

• Proceedings of the KIEE Conference
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• 1999.11c
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• pp.585-587
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• 1999

The rotorbody and rotor winding of generator are isolated by an insulator and the output characteristic of the generator is maintained in the best states. Only when an insulation resistance between them is over a certain extent. The aim of this research is to develop the simulator for rotor earth fault detection circuits. It is composed of the power resource which is to control the virtual field voltage, stepping motor which is to give virtual ground. It is possible to inspect with the device and program developed in this study in the same as real operating condition and evaluate the integrity of generator rotor through the function of data acquisition and graphic output. If these technologies will be applied to the inspection, prevent a damage of the generator and contribute to improve maintenance reliance.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.58 no.3
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• pp.276-284
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• 2009

In this paper, we develop the image transfer remote maintenance monitoring system for hand held device which can compensate defects of human mistake. The human mistakes always happen when the worker communicate information each other to check and maintenance the equipment of the power plant under bad circumstance such as small place and long distance in power plant. A worker couldn't converse with other when in noisy place like Power plant. So, we make some hand device for handy size and able to converse in noisy place. The developed system can have improvement of productivity through increasing plant operation time. And developed system is composed of advanced H/W(hard ware) system and S/W(soft ware)system. The H/W system consist of media server unit, communication equipment with hand held device, portable camera, mike and head set. The advanced s/w system consist of data base system, client pc(personal computer) real time monitoring system which has server GUI(graphic user interface) program, wireless monitoring program and wire ethernet communication program. The client GUI program is composed of total solution program as pc camera program, and phonetic conversation program etc.. We analyzed the required items and investigated applicable part in the image transfer remote maintenance monitoring system with hand held device. Also we investigated linkage of communication protocol for developed prototype, developed software tool of two-way communication and realtime recording skill of voice with image. We confirmed the efficiency by the field test in preventive maintenance of plant power.

• IE interfaces
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• v.10 no.1
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• pp.237-248
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• 1997

The purpose of this study is to make an economic analysis of power plant utilities by examining electricity generating costs with environmental consideration. Economic growth has caused pollutant emission, and subsequent environmental pollution has been identified as a very real limit to sustainable development. Considering the enormous role of electricity in the national economy, it is thus very important to study the effect of environmental regulations on the electricity sector. Because power utilities need large investments during construction, operation and maintenance, and also require much construction lead time. Economic analysis is the very important process in the electric system expansion planning. In this study, the levelized generation cost method is used in comparing economic analysis of power plant utilities. Among the pollutants discharged of the electricity sector, this study principally deals with the control activities related only to $CO_2$, and $NO_2$, since the control cost of $SO_2$, and TSP (Total Suspended Particulates) is already included in the construction cost of utilities. The cost of electricity generation in a coal-fired power plant is compared with one in an LNG combined cycle power plant. Moreover this study surveys the sensitivity of fuel price, interest rate and carbon tax. In each case, this sensitivity can help to decide which utility is economically justified in the circumstance of environmental regulations.

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.366-372
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• 2009

This study was conducted to develop new lines expressing the characteristic of early flowering by introducing MdMADS2 gene in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) ‘Zinba'. Transformation of chrysanthemum was conducted by Agrobacterium tumefaciens LBA4404 harboring the binary vector containing MdMADS2 controlled by double CaMV 35S promoters. Ninety three shoots were regenerated from 1,463 leaf segment explants cultured on the first selection medium (MS basal salts + 1.0 mg/L BA + 0.5 mg/L IAA + 10 mg/L kanamycin + 400 mg/L cefotaxime, pH 5.8) after co-cultivation, and 20 out of the 93 shoots rooted on the second selection medium containing 20 mg/L kanamycin and 400 mg/L cefotaxime. Many escapes (98.6%) were removed on the selection stage for rooting. Nineteen lines were confirmed as transgenic plant with transgene by PCR analysis. Six transgenic plants flowered 2-11 days earlier than non-transgenic plant without big change of phenotype, and especially, 3 (Mo-7, Mo-11, Mo-17) out of 6 transgenic lines showed a significant reduction in days to flowering compared to non-transgenic plant. Introduction and expression of MdMADS2 gene in them were confirmed by Southern and real-time PCR analyses, respectively.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.3-9
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• 2008

Recently, the plant industries are being activated and plant control systems use various technologies. Because the optimized design for the plants is very important for the reducing of operation and maintenance costs, automatic control systems become more important. Plant control systems consist of the master controller, the plant networks, the programming environment for engineering, monitoring software and the field devices. The control systems should have reliability, availability and safety. Modular architecture of hardware and software makes flexible configuration of the control systems. Each component should have diagnostic functions. It follows industrial standards and makes open systems. Open systems increase accessibility against the data which is distributed in the plants. The controllers including processor and communication modules use the up-to-date technology. They have real time and fault tolerant function by duplicating processors or networks. It also enables to make the distributed control systems. The distributed architecture makes more scalable main control system. Automatic control systems can be operated with better performance. In this paper, we analyzed the requirements of the seawater desalination plants and made some consideration facts for developing the optimized controller. Also we described the design concept of the main controller, which consists of several modules. We should validate and complement the design for the reliability and better performance.