The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Tomato Yellow Leaf Curl China Virus Impairs Photosynthesis in the Infected Nicotiana benthamiana with βC1 as an Aggravating Factor

  • Farooq, Tahir (State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences) ;
  • Liu, Dandan (State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences) ;
  • Zhou, Xueping (State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences) ;
  • Yang, Qiuying (State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant Protection, China Academy of Agricultural Sciences)
  • Received : 2019.04.27
  • Accepted : 2019.07.18
  • Published : 2019.10.01
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Abstract

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

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References

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