• 생약학회지
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• 제40권2호
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• pp.103-108
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• 2009

GCSB-5 preparation is a purified extract from a mixture of 6 medicinal plants(Acanthopanacis Cortex, Achyranthis Radix, Saposhnikoviae Radix, Cibotii Rhizoma, Glycine Semen Nigra, Eucommiae Cortex) that have been widely used for the treatment of various bone disorders. The aim of this study was to investigate HPLC analysis method and screening of standard compound on Saposhnikoviae Radix for quality standardization of a medicinal crude drug GCSB-5. Standard compound of Saposhnikoviae Radix was decided with cimifugin by isolation and instrumental analysis such as NMR. HPLC analysis method for the simultaneous determination of cimifugin was established for the quality control of the medicinal plants of Saposhnikoviae Radix species, GCSB-5 raw material and preparation. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline.

• 대한한의학방제학회지
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• 제14권1호
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• pp.11-29
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• 2006

This report describes 71 studies related to the use of Radix Saposhnikoviae main blended prescription from Donguibogam. The following conclusion were reached through investigations on the prescriptions that use Radix Saposhnikoviae as a main ingredient. Prescriptions that Radix Saposhnikoviae was taken as a monarch drug are utilized for 20 therapeutic purposes, for example, eye disease, wind disease, head disease and Sang Han syndromes. In particular, 28.1% of prescriptions appear in the chapter of eye, and 25.3% of those appear in the chapter of head. Prescriptions that utilize Radix Saposhnikoviae as the main ingredient are used in the treatment of eye disease. paralysis and tetanus. Radix Saposhnikoviae is used in pathogenic factors such as wind. wind with cold. and used in pathology related to liver system. The dosage of Radix Saposhnikoviae in prescriptions is 7 li(about 0.26 gram) to 2.5 jeon(about 9.38 gram), however 1 jeon(about 3.75 gram) has been taken the most for clinical application. Bangpungtang is the most useful base prescription which use the Radix Saposhnikoviae as the main ingredient.

• 대한본초학회지
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• 제27권5호
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• pp.85-91
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• 2012

Objectives : In this study, we investigated the effect of Saposhnikoviae Radix on allergic responses in ovalbumin(OVA)-induced Allergic rhinitis(AR) mice. Methods : BALB/c mice were orally administrated with Saposhnikoviae Radix water extract (SRW, 50 mg/kg) or anti-histamine drug, Ketotifen (10 mg/kg) as a reference drug, followed by sensitization and challenge of OVA. Mice were measured clinical symptoms and the serum levels of histamine, IgE, IL-4, and IFN-${\gamma}$, and observed histopathological changes of nasal mucosa H&E staining. Results : SRW significantly decreased rubs and the serum levels of histamine, IgE, and IL-4, and then increased the serum levels of IFN-${\gamma}$ in OVA-induced AR mice, and inhibited histopathological changes of nasal mucosa with inflammation and the eosinophils infilteration. Conclusions : These data suggest that SRW has anti-allergic effect through the inhibitory property of Saposhnikoviae Radix against allergic responses in allergic rhinitis.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.393-399
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• 2020

방풍(Saposhnikoviae Radix, SR) 과 식방풍(Peucedani Japonici Radix, PR)은 각각 다른 기원식물을 가지며 속과 종이 다르지만 한국, 중국, 및 일본에서 대표적인 전통 한약재로 오랫동안 혼용되어 사용되었다. 본 연구에서는 방풍과 식방풍의 판별 마커를 확인하기 위하여 UPLC-QTOF/MS를 이용한 대사체 프로파일링 및 다변량 통계분석을 진행하였다. 그 결과, 5-O-methylvisammioside와 peucedanol을 각각 방풍과 식방풍의 지표성분으로 선정하였으며, UPLC를 이용하여 분석법을 검증하였다. 제안된 방풍 및 식방풍의 지표성분에 대한 분석법 검증은 방풍과 식방풍의 분류와 품질 평가 및 성분 검증을 위한 효과적인 방법이 될 것으로 기대된다.

• Toxicological Research
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• 제35권4호
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• pp.371-387
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• 2019

Although the dried root of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae) is a popular medicinal plant in East Asia, there has been no systemic toxicological evaluation of a water extract of Saposhnikoviae Radix (SRE). In this experiment, an oral acute and 13-week subchronic toxicological evaluations of SRE (500-5,000 mg/kg body weight) were performed in both sexes of Crl:CD(SD) rats. Based on the results from mortality, clinical signs, effects on body weight and organ weight, clinical biochemistry, hematology, urinalysis, and histopathology, significant acute, 4-week repeated dose range finding (DRF) and 13-week subchronic toxicity of SRE was not observed in either sex of rats; thus, the no observed adverse effect level (NOAEL) was 5,000 mg (kg/day). To identify anti-hyperuricemia potential of SRE, the suppressive effect of SRE was determined in mice challenged with potassium oxonate (PO; 250 mg/kg) via intraperitoneal injection for 8 days (each group; n = 7). SRE supplementation suppressed the uric acid level in urine through significant xanthine oxidase (XO) inhibitory activity. Kidney dysfunctions were observed in PO-challenged mice as evidenced by an increase in serum creatinine level. Whereas, SRE supplementation suppressed it in a dose-dependent manner. Collectively, SRE was safe up to 5,000 mg (kg/day) based on NOAEL found from acute and 13-week subchronic toxicological evaluations. SRE had anti-hyperuricemia effect and lowered the excessive level of uric acid, a potential factor for gout and kidney failure.

• 동의생리병리학회지
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• 제19권3호
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• pp.710-715
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• 2005

This research was to investigate inhibitory activity of Sasang medicines on Obesity. 56 kind of herb medicines to powder, abstract add 100 times methanol. Examine inhibitory effect against lipase activity in vitro. It was compared Xenical(Orlistat) with 56 kind of herb medicines. Inhibitory effect against lipase activity of Xenical as a standard was 93.3%. It is Inhibitory effect against lipase activity of Taeyangin herb medicine. (Fructus Pruni Tomentosae 77.7% Cortex Acanthopanacis73.5%, Fructus Chaenomelis73.1%, rice bran on a mallet head68.1%, Semen Fagopyri 48.1%, Radix Vitis 31.5%) It is Inhibitory effect against lipase activity of Soyangin herb medicine. (Radix Saposhnikoviae 91.5%, Semen Plantaginis 90.4%, Semen Trichosanthis 89.2%, Herba Schizonepetae 85.7%, Radix Euphorbiae Kansui 76.1%, Rhizoma Anemarrhenae 76.0%, Cortex Phellodendri 75.1%, Herba Menthae 74.8%, Radix Angelicae Pubescentis 61.2%, Rhizoma Alismatis 62.6%, Poria 60.9%, Rhizoma Notopterygii 22.5%, Radix Peucedani 18.2%, Caulis Akebiae 17.7%) It is Inhibitory effect against lipase activity of Taeeumin herb medicine. (Herba Spirodelae 91.4%, Radix Polygalae 88.3%, Arillus Longanae 84.3%, Radix Platycodi 81.7%, Semen Zizyphi Spinosae 80.2%, Spina Gleditsiae 79.7%, Herba Ephedrae 75.3%, Semen Raphani 73.1%, Rhizoma Cimicifugae 73.0%, Rhizoma Acori Graminei 71.8%, Flos Chrysanthemi71.0%, Radix et Rhizoma Rhei 68.4%, Vermiculus Holotrichia 59.9%, Radix Puerariae 55.3%, Fructus Schizandrae 53.4% Semen Coicis 50.6%, Semen Biotae 47.5%, Semen Nelumbinis 46.7%, Radix Angelicae Dahuricae 45.1%, Semen Castaneae 44.4%, Rhizoma Ligustici 34.5%, Tuber Liriopis 16.4%, Radix Scutellariae4.2%) It is Inhibitory effect against lipase activity of Soeumin herb medicine. (Pericarpium Arecae89.9%, Rhizoma Alpiniae Officinarum 89.5%, Radix Polygoni Multiflori81.2%, Rhizoma Cyperi 79.0%, Cortex Magnoliae 72.8%, Radix Aucklandiae 72.0%, Rhizoma Zingiberis 71.9%, Pericarpium Citri Reticulatae Viride 71.3%, Radix Cynanchi Wilfordi 57.7% Rhizoma Pinelliae 36.7%, Fructus Alpiniae Oxyphyllae 35.8% Fructus Aurantii Immaturus 17.5%, Pericarpium Citri 16.1%) Therefore, Radix Saposhnikoviae(91.5) and Herba Spirodelae(91.4%) were the most effective medicice of 56 kind of medicines.

• 대한한의학방제학회지
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• 제31권3호
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• pp.171-182
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• 2023

Objectives : Saposhnikoviae Radix (SR) and Glehniae Radix (GR) have been frequently used in traditional medicine to treat diseases related to 'wind' syndrome, but there have been cases where it has been mixed in a state where the plant of origin is not clear. In this study, to select materials for conducting preclinical cerebral infarction research, the network pharmacology analysis method was used to select suitable medicinal materials for the study. Methods : In this study, a Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) based network pharmacology analysis method was used, and oral bioavailability (OB), drug likeness (DL), Caco-2 and BBB permeability were utilized to select compounds with potential activity. For the values of each variable used in this study, OB ≥ 20%, DL ≥ 0.18, Caco-2 ≥ 0, and BBB ≥ -0.3 were applied, then networks of bioactive compounds, target proteins, and target diseases was constructed. STRING database was used to construct a protein-protein interaction network. Results : It was confirmed that SR rather than GR has various target proteins and target diseases based on network pharmacological analysis using TCMSP database. And it was analyzed that the bioactive compounds only in SR act more on neurovascular diseases, and both drugs are expected to be effectively used for cardiovascular diseases. Conclusions : In our future study, SR will be used in an ischemic stroke mouse model, and the mechanism of action will be explored focusing on apoptosis and cell proliferation.

• 한국식품과학회지
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• 제40권2호
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• pp.135-140
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• 2008

방풍, 시호, 천궁, 황기, 한약재 국산과 수입산 307점을 대상으로 분석과정에서 특별한 전처리 과정이 필요없는 간편하고 신속한 비파괴 분석법을 사용하여 식물 생장에 필수 원소인 다량 무기질 P, S, K, Ca의 함량을 EDXRF로 측정하고 정준판별분석을 실시한 후 원산지 판별 가능성을 검토하였다. 국산 방풍의 P, S, K, Ca 함량은 평균 12.67%, 7.51%, 60.21%, 19.61%이었고 수입산은 평균 9.72%, 20.66%, 50.29%, 19.33%였으며 국산 방풍에는 P, K 함량이 수입산에 비하여 높게 존재하였고 S는 수입산에 많았으며 Ca는 유사하였고 전체 75점의 시료 중 70점의 원산지를 정확히 판별하여 93.3%의 매우 높은 판별정확도를 나타냈다.국산 시호의 P, S, K, Ca 함량은 평균 8.91%, 6.42%, 50.18%, 34.48%였고 수입산 시호는 평균 4.35%, 12.16%, 43.40%, 40.09%이었다. 국산 시호에는 P, K 함량이 수입산보다 높았으며 S와 Ca는 수입산에서 더 높은 함량을 보였다. 국산과 수입산의 판별 정확도는 전체 70점의 분석 시료 중 67점의 원산지를 정확히 판별하여 95.7%의 높은 정확도를 나타냈다. 국산 천궁 P, S, K, Ca 함량은 평균 8.71%, 7.04%, 65.91%,18.33%이었고 수입산 천궁은 평균 11.05%, 13.79%, 51.48%, 23.69%의 함량을 나타내었다. K 함량은 국산 천궁에서 수입산보다 높은 함량을 나타냈고 P, S, Ca는 수입산에서 더 높은 함량을 보여 전체 82점의 시료 중 81점의 원산지를 정확히 판별하여 98.8%의 가장 높은 판별정확도를 나타냈다. 국산 황기의 P, S, K, Ca 함량은 평균 15.52%, 17.38%, 54.74%, 12.36%이고 수입산은 14.20%, 25.59%, 45.16%, 15.05%이었다. 국산에서 K함량이 수입산보다 높은 함량을 보였으며 S, Ca는 수입산 에서 국산보다 높은 함량을 나타내었고 P는 큰 차이가 없이 유사하였다. 전체 80점의 황기를 분석하여 70점의 원산지를 정확하게 판별하여 87.5%의 판별정확도를 보였다. 결론적으로 방풍, 시호, 천궁, 황기 모두에서 국산과 수입산간에 높은 판별정확도를 나타내 원산지 판별이 가능함을 알 수 있었다.

• 대한한방소아과학회지
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• 제31권1호
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• pp.1-11
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• 2017

Objectives The purpose of this study is to investigate commonly-used-pattern-identifications and to understand herbal medicine treatment for psoriasis based on recent clinical studies. Methods Keyword 'psoriasis' was used to search articles in National Digital Science Library (NDSL), Korean Traditional Knowledge Portal (KTKP), Oriental Medicine Advanced Searching Integrated System (OASIS) and Research Information Sharing Service (RISS). 19 relevant articles were reported between 2006 - 2016, and were obtained and reviewed. Results Among the 19 articles, the most commonly-used-pattern-identifications were 'blood heat pattern'. 'Gunsun-bang', 'Bangpungtongsungsan-gami'. Also, based on the search results, 'Yangdokbaekho-tang' were more frequently prescribed than other herbal medicines, and ingredients such as, Glycyrrhizae Radix (甘草), Saposhnikoviae Radix (防風) and Rehmanniae Radix (生地黃) were used repeatedly in those prescriptions. Conclusions This study showed what pattern identifications there are, and what herbal medicines are often used in clinical treatments. Developing new form of herbal medicines are also going to be possible with further research.

• 대한본초학회지
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• 제29권3호
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• pp.71-78
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• 2014

Objectives : This study evaluates the neuroprotective effects of Sopung-tang, a mixture of Notopterygii Rhizoma, Saposhnikoviae Radix, Angelicae Gigantis Radix, Cnidii Rhizoma, Hoelen, Aurantii Nobilis Pericarpium, Pinelliae Tuber, Linderae Radix, Angelicae Dahuricae Radix, Cyperi Rhizoma, Cinnamomi Ramulus, Asari Radix, Glycyrrhizae Radix on the cerebral infarct combined with hyperlipidemia. Method : The hyperlipidemia was induced by the beef tallow 30% diet for 14 days on Sprague-Dawley rats. The cerebral infarct was induced by the middle cerebral artery occlusion (MCAO) for 2 hours with intraluminal thread method. Then the water extract of Sopung-tang was administered a day for 5 days at 3 hours after the cerebral infarct by MCAO. Effect of Sopung-tang was evaluated with the infarct volume and edema percentage by a TTC-stained brain section, and the expressions of Bax and Bcl-2 in the brain tissue by a immunohistochemical stain method. Results : Sopung-tang reduced the infarct size partly in a TTC-stained brain section of the hyperlipidemic MCAO rats. Sopung-tang reduced the infarct volume of the hyperlipidemic MCAO rats significantly. Sopung-tang reduced the edema percentage of the hyperlipidemic MCAO rats, but not significant statistically. Sopung-tang suppressed the Bax expressions in the cerebral penumbra and caudate putamen of the hyperlipidemic MCAO rats significantly. Sopung-tang upregulated the Bcl-2 expression in the caudate putamen of the hyperlipidemic MCAO rats. Conclusion : These results suggest that Sopung-tang plays an anti-apoptotic neuroprotective effect through the suppression of Bax and up-regulation of Bacl-2 expressions in the brain tissues.