• 한국응급구조학회지
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• 제9권1호
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• pp.5-13
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• 2005

Purpose : The purpose of this study was to investigate dual gender identity, masculine gender identity, feminine gender identity, undifferentiated gender identity with related to Sex, home environments, parental child-rearing attitude of warmth, parental child-rearing attitude of control, role division of parents and determine predictors for dual gender identity. Method : Study subjects were $6^{th}$ grade of primary students with 2,118. Data was collected from Oct to Nov 2003 by using structured questionnaire. Results : ${\cdot}$ Among the children, 345 had masculine gender identity, 529 had feminine gender identity, 526 had undifferentiated gender identity, and 718 had dual gender identity. ${\cdot}$ There were significant differences in the children's sex, mother's age, father's age, mother's educational level, father's educational level, existence of mother's job, father's job, social economic status, sex of siblings, mother's job satisfaction, family structure, family atmosphere, child-rearing attitude(warmth and control), role division of parents($p{\leq}0.001$) among 4 groups. ${\cdot}$ The significant predictors for dual gender identity were children's sex (OR = 0,196, P =0.001), father's age(OR = 31.053, p = 0.020), mother's educational level(OR = 43,980, p = 0.001), father's job(OR=27.465, p = 0.001), social economic status(OR=O.941, p=0.001), sex of siblings(OR = 0.329, p = 0.005), mother's job satisfaction(OR = 0.673, p =0.001), family structure(OR = 0.887, p = 0.001), family atmosphere(OR = 23.786, p = 0.001), parental Child-rearing attitude of warmth(OR = 8.043, p = 0.001) and child-rearing attitude of control(OR = 0.666, p = 0.005), role division of parents(OR = 3.009, p = 0.001). Conclusions : These findings suggest the necessity of broad understandings about factors which influence dual gender role, and construction of combinative model. Also they suggest parent education for establishment of children's dual gender identity.

• 농업과학연구
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• 제43권1호
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• pp.52-60
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• 2016

In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-${\beta}$ superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.83.2-83
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• 2003

A full length cDNA, OsLEUZIP, encoding leucine zipper containing protein from rice EST of rice (0ryza sativa L. cv. Dongjin) treated Xanthomonas oryzae pv. oryzae 10331. OsLEUZIP contains 1,227 bp nucleotides and encodes a protein of 408 amino acid residues with predicted molecular weight of 47,229 Da. The deduced amino acid sequence of OsLEUZIP has consensus sequence of leucine zipper from PROSITE (PDOC00029), L-X(6)-L-X(6)-L-X(6) -L. OsLEUZIP gene were preferentially induced in rice during incompatible interaction with Xanthomonas oryzae pv. oryzae 10331 and Pyracuraria grisea KJ-301. Expression of OsLEUZIP gene was also induced by treatment of abiotics such as ethephon and ABA. Our data represented in this study suggesting that OsLEUZIP gene may play an important role in the rice defense-related. Further studies of this gene, overexpression in rice, yeast-two hybrid assay, electrophoretic mobility shift assay and northern blot analyses of transgenic plant, would be useful to elucidate the role of the OsLEUZIP gene in defense responses of rice.

• BMB Reports
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• 제42권10호
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• pp.685-690
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• 2009

Angiogenesis is essential for tumor growth and vascular endothelial cell growth factor (VEGF) plays a key role in this process. Conversely, sphingosine 1-phosphate (S1P) is a biologically active sphingolipid known to play a key role in cancer progression by regulating endothelial cell proliferation and migration. In this study, the authors found that S1P increases the level of VEGF mRNA in human umbilical vein endothelial cells (HUVECs) and immortalized HUVECs (iHUVECs). Additionally, S1P was found to increase VEGF promoter activity in MS-1 mouse pancreatic islet endothelial cells. Furthermore, a pharmacological inhibitory study revealed that $G_{\alpha i/o}$-mediated phospholipase C, Akt, Erk, and p38 MAPK signaling are involved in this S1P-induced expression of VEGF. A component of AP1 transcription factor is important for S1P-induced VEGF expression. Taken together, these findings suggest that S1P enhances endothelial cell proliferation and migrat ion by upregulating the expression of VEGF mRNA.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.251-258
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• 2014

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

• 한국패류학회지
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• 제32권4호
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• pp.241-247
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• 2016

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2005년도 가을 학술발표논문집 Vol.32 No.2 (1)
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• pp.94-96
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• 2005

기존의 역할기반 접근통제(RBAC: Role Based Access Control) 모델은 역할(R), 사용자(U), 접근권한 할당(A), 접근권한의 상속성의 관계에 의해 접근통제를 실행할 때. 하위역할에 할당된 사용자의 모든 접근권한은 상위역할에 할당된 사용자의 접근권한에 상속됨으로서 권한의 집중으로 인하여 최소권한 정책을 위배하고, 권한의 남용문제가 발생한다. 본 논문에서는 기존의 RBAC 모델에서 제기되는 문제를 해결하기 위하여 역할에 보안등급(허용등급, 허용범위)을 이용하여 접근권한의 상속성을 제한하고, 속(보안등급, 부분순서)의 이론을 적용하여 접근권한의 흐름을 통제할 수 있는 역할계층 접근통제 모델을 제안하였다. 제안한 모델은 역할의 특정영역을 지정함으로서 부분적인 역할계층을 구성할 수 있기 때문에 새로운 역할을 추가하거나 제거를 용이하게 할 수 있고, 역할의 계층구조를 간편하게 갱신할 수 있게 항으로서 다단계 보안시스템에서도 효과적으로 접근통제를 할 수 있을 뿐 아니라 대규모 보안시스템으로 확장할 수 있는 장점을 갖는다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.479-487
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• 2014

Leptin plays an important role in energy homeostasis and reproductive function in fish, especially in reproduction. Migrating fish, such as salmonoids, are affected by external environmental factors, and salinity changes are a particularly important influence on spawning migrations. The aim of this study was to test whether changes in salinity affect the expression of leptin, estrogen receptors (ERs), and vitellogenin (VTG) in chum salmon (Oncorhynchus keta). The expression and activity of leptin, the expression of ERs and VTG, and the levels of estradiol-$17{\beta}$ and cortisol increased after the fish were transferred to FW, demonstrating that changes in salinity stimulate the HPG axis in migrating female chum salmon. These findings reveal details about the role of elevated leptin levels and sex steroid hormones in stimulating sexual maturation and reproduction in response to salinity changes in chum salmon.

• Mass Spectrometry Letters
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• 제7권2호
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• pp.31-40
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• 2016

The analysis of nuclear materials and environmental samples is an important issue in nuclear safeguards and nuclear forensics. An analysis technique for safeguard samples has been developed for the detection of undeclared nuclear activities and verification of declared nuclear activities, while nuclear forensics has been developed to trace the origins and intended use of illicitly trafficked nuclear or radioactive materials. In these two analytical techniques, mass spectrometry has played an important role in determining the isotope ratio of various nuclides, contents of trace elements, and production dates. These two techniques typically use similar analytical instruments, but the analytical procedure and the interpretation of analytical results differ depending on the analytical purpose. The isotopic ratio of the samples is considered the most important result in an environmental sample analysis, while age dating and impurity analysis may also be important for nuclear forensics. In this review, important aspects of these techniques are compared and the role of mass spectrometry, along with recent progress in related technologies, are discussed.

• Carbon letters
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• 제3권4호
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• pp.192-197
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• 2002

Microstructure plays an important role in controlling the fracture behaviour of carbon-carbon composites and hence their mechanical properties. In the present study effort was made to understand how the different interfaces (fiber/matrix interactions) influence the development of microstructure of the matrix as well as that of carbon fibers as the heat treatment temperature of the carbon-carbon composites is raised. Three different grades of PAN based carbon fibres were selected to offer different surface characteristics. It is observed that in case of high-strength carbon fiber based carbon-carbon composites, not only the matrix microstructure is different but the texture of carbon fiber changes from isotropic to anisotropic after HTT to $2600^{\circ}C$. However, in case of intermediate and high modulus carbon fiber based carbon-carbon composites, the carbon fiber texture remains nearly isotropic at $2600^{\circ}C$ because of relatively weak fiber-matrix interactions.