Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii)

  • Kim, Ju Lan (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kong, Hee Jeong (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Hyung Soo (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Woo-Jin (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Dong-Gyun (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Nam, Bo-Hye (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • Kim, Young-Ok (Biotechnology Research Division, National Fisheries Research and Development Institute) ;
  • An, Cheul Min (Biotechnology Research Division, National Fisheries Research and Development Institute)
  • Received : 2014.11.10
  • Accepted : 2014.11.24
  • Published : 2014.12.31
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Abstract

Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designated RuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-rich region at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other species including human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression of RuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA was detected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Further investigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.

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References

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