The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
권호 표지
DOI QR코드

DOI QR Code

Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the pacific abalone (Haliotis discus hannai)

북방전복 (Haliotis discus hannai) 의 대식세포이동저해인자 (MIF, Macrophage migration inhibitory factor) 유전자 동정 및 발현분석

  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Macrophage Migration Inhibitory Factor (MIF) are well-defined role as unique cytokine and critical mediator in acute and chronic inflammatory diseases, autoimmune diseases. In this study, we isolated and characterized a full-length of MIF cDNA from the abalone (Haliotis discus hannai). The full-length cDNA of abMIF was of 1264 bp, consisting of a 5'-terminal UTR of 143 bp, an open reading frame of 360 bp and a 3-terminal UTR of 761 bp. The abalone MIF cDNA encodes a 119-amino acid polypeptide with a calculated molecular mass of 13.4 kDa and isoelectric point of 9.07. Multiple alignments and phylogenetic analysis with the deduced abalone MIF protein and showed strong homology with disk abalone (Haliotis discusdiscus). The deduced amino acid sequence of abMIF exhibited homology with other reported MIFs, such as 80%, with that of other disk abalone H. discus discus MIF gene. Quantitative real-time PCR (qRT-PCR) analysis indicated that abMIF was highly expression observed in hapatopacreas, intestine, foot, and gonad of normal conditioned abalone. Even though AbMIF mRNA level in hemocytes was low under the normal condition, it was sharply up-regulated and reached the maximum at 6 h post-infection with Vibrio parahaemolyticus, and then decreased at 24 h post-infection. This result indicates that abMIF plays an important role in responding in the innate immune system.

Keywords

References

  1. Abe, R., Peng, T., Sailors, J., Bucala, R. and Metz, C.N. (2001) Regulation of the CTL responseby macrophage migration inhibitory factor. The Journal of immunology, 166: 747-753. https://doi.org/10.4049/jimmunol.166.2.747
  2. Baeza Garcia, A., Pierce, R.J., Gourbal, B., Werkmeister, E., Colinet, D., Reichhart, J.M., Dissous, C. and Coustau, C. (2010) Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosomamansoni. PLoS pathogens, 6: e1001115. https://doi.org/10.1371/journal.ppat.1001115
  3. Bacher, M., Meinhardt, A., Lan, H.Y., Mu, W., Metz, C.N., Chesney, J.A., Calandra, T., Gemsa, D., Donnelly, T., Atkins, R.C. and Bucala, R. (1997) Migration inhibitory factor expression in experimentally induced endotoxemia. The American journal of pathology, 150: 235-246.
  4. Bernhagen, J., Calandra, T., Mitchell, R.A., Martin, S.B., Tracey, K.J., Voelter, W., Manogue, K.R., Cerami, A. and Bucala, R. (1993) MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature, 365: 756-759. https://doi.org/10.1038/365756a0
  5. Bloom, B.R. and Bennett, B. (1966) Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science, 153: 80-82. https://doi.org/10.1126/science.153.3731.80
  6. Calandra, T., Bernhagen, J., Metz, C.N., Spiegel, L.A., Bacher, M., Donnelly, T., Cerami, A. and Bucala, R. (1995) MIF as a glucocorticoid-induced modulator of cytokine production. Nature, 377: 68-71. https://doi.org/10.1038/377068a0
  7. Cui, S., Zhang, D., Jiang, S., Pu, H., Hu, Y., Guo, H., Chen, M., Su, T. and Zhu, C. (2011) Amacrophage migration inhibitory factor like oxidoreductase from pearl oyster Pinctadafucata involved in innate immune responses. Fish and shellfish immunology, 31: 173-181. https://doi.org/10.1016/j.fsi.2011.03.009
  8. David, J.R. (1966) Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proceedings of the National Academy of Sciences of the United States of America, 56: 72-77.
  9. Du, J., Xie, X., Chen, H., Yang, W., Dong, M., Su, J., Wang, Y., Yu, C., Zhang, S. and Xu, A. (2004) Macrophage migration inhibitory factor (MIF) in Chinese amphioxus as a molecular marker of immune evolution during the transition of invertebrate/ vertebrate. Developmental and comparative immunology, 28: 961-971. https://doi.org/10.1016/j.dci.2004.04.001
  10. Itoa, K., Yoshiura, Y., Ototake, M. and Nakanishi, T. (2008)Macrophage migration inhibitoryfactor (MIF) is essential for development of zebrafish, Daniorerio. Developmental and comparative immunology, 32: 664-672. https://doi.org/10.1016/j.dci.2007.10.007
  11. Jaworski, D.C., Jasinskas, A., Metz, C.N., Bucala, R. and Barbour, A.G. (2001) Identification and characterization of a homologue of the pro-inflammatory cytokine Macrophage Migration Inhibitory Factor in the tick, Amblyomma americanum. Insect molecular biology, 10: 323-331. https://doi.org/10.1046/j.0962-1075.2001.00271.x
  12. Jin, H.J., Xiang, L.X. and Shao, J.Z. (2007) Molecular cloning and identification of macrophage migration inhibitory factor (MIF) in teleost fish. Developmental and comparative immunology, 31: 1131-1144. https://doi.org/10.1016/j.dci.2007.02.004
  13. Karmir, D., Zierow, S., Leng, L., Cho, Y., Diaz, Y., Griffith, J., McDonald, C., Merk, M., Mitchell, R.A., Trent, J., Chen, Y., Kwong, Y.K., Xiong, H., Vermeire, J., Cappello, M., McMahon-Pratt, D., Walker, J., Bernhagen, J., Lolis, E. and Bucala, R. (2008) A Leishmaniaortholog of macrophage migration inhibitory factor modulates host macrophage responses. The Journal of immunology, 180: 8250-8261. https://doi.org/10.4049/jimmunol.180.12.8250
  14. Kim, S., Miska, K.B., Jenkins, M.C., Fetterer, R.H., Cox, C.M., Stuard, L.H. and Dalloul, R.A. (2010) Molecular cloning and functional characterization of the avian macrophage migrationinhibitory factor (MIF). Developmental and comparative immunology, 34: 1021-1032. https://doi.org/10.1016/j.dci.2010.05.005
  15. Li, Y., Messina, C., Bendaoud, M., Fine, D.H., Schreiner, H. and Tsiagbe, V.K. (2010) Adaptive immune response in osteoclastic bone resorption induced by orally administered Aggregatibacter actinomycetemcomitans in a rat model of periodontal disease. Molecular oral microbiology, 25: 275-292. https://doi.org/10.1111/j.2041-1014.2010.00576.x
  16. Livak, K.J. and Schmittgen, T.D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-{\Delta}{\Delta}CT }$ method. Methods, 25: 402-408. https://doi.org/10.1006/meth.2001.1262
  17. Lue, H., Kleemann, R., Calandra, T., Roger, T. and Bernhagen, J. (2002) Macrophage migration inhibitory factor (MIF): mechanisms of action and role in diseas. Microbes and infection, 4: 449-460. https://doi.org/10.1016/S1286-4579(02)01560-5
  18. Marson, A.L., Tarr, D.E. and Scott, A.L. (2001) Macrophage migration inhibitory factor (mif) transcription is significantly elevated in Caenorhabditis elegans dauer larvae. Gene, 278: 53-62. https://doi.org/10.1016/S0378-1119(01)00706-5
  19. Miska, K.B., Fetterer, R.H., Lillehoj, H.S., Jenkins, M.C., Allen, P.C. and Harper, S.B. (2007) Characterisation of macrophage migration inhibitory factor from Eimeria species infectious to chickens. Molecular and biochemical parasitology, 151: 173-183. https://doi.org/10.1016/j.molbiopara.2006.10.020
  20. Onodera, S., Suzuki, K., Matsuno, T., Kaneda, K., Takagi, M. and Nishihira, J. (1997) Macrophage migration inhibitory factor induces phagocytosis of foreign particles by macrophages in autocrine and paracrine fashion. Immunology, 92: 131-137. https://doi.org/10.1046/j.1365-2567.1997.00311.x
  21. Parisi, M.G., Toubiana, M., Mangano, V., Parrinello, N., Cammarata, M. and Roch, P. (2012) MIF frommussel: coding sequence, phylogeny, polymorphism, 3D model and regulation of expression. Developmental and comparative immunology, 36: 688-696. https://doi.org/10.1016/j.dci.2011.10.014
  22. Suzuki, M., Takamura, Y., Maeno, M., Tochinai, S., Iyaguchi, D., Tanaka, I., Nishihira, J. and Ishibashi, T. (2004) Xenopuslaevis macrophage migration inhibitory factor is essential for axis formation and neural development. The Journal of biological chemistry, 279: 21406-21414. https://doi.org/10.1074/jbc.M311416200
  23. Tan, T.H., Edgerton, S.A., Kumari, R., McAlister, M.S., Roe, S.M., Nagl, S., Pearl, L.H., Selkirk, M.E., Bianco, A.E., Totty, N.F., Engwerda, C., Gray, C.A. and Meyer, D.J. (2001) Macrophage migration inhibitory factor of the parasitic nematode Trichinellaspiralis. The Biochemical journal, 357: 373-383. https://doi.org/10.1042/bj3570373
  24. Wang, B., Zhang, Z., Wang, Y., Zou, Z., Wang, G., Wang, S., Jia, X. and Lin, P. (2009) Molecular cloning and characterization of macrophage migration inhibitory factor from small abalone Haliotis diversicolor supertexta. Fish and shellfish immunology, 27: 57-64. https://doi.org/10.1016/j.fsi.2009.04.004
  25. Wang, F., Shen, X., Guo, X., Peng, Y., Liu, Y., Xu, S. and Yang, J. (2010) Spinal macrophage migration inhibitory factor contributes to the pathogenesis of inflammatory hyperalgesia in rats. Pain, 148: 275-283. https://doi.org/10.1016/j.pain.2009.11.011
  26. Xie, B., Fu, M., Zhao, C., Shi, J., Shi, G., Jiao, Z. and Qiu, L. (2016). Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the black tiger shrimp (Penaeusmonodon). Fish and shellfish immunology, 56: 489-495. https://doi.org/10.1016/j.fsi.2016.08.008
  27. Zang, X., Taylor, P., Wang, J.M., Meyer, D.J., Scott, A.L., Walkinshaw, M.D. and Maizels, R.M. (2002) Homologues of Human Macrophage Migration Inhibitory Factor from a Parasitic Nematode. The Journal of biological chemistry, 277(46): 44261-44267. https://doi.org/10.1074/jbc.M204655200