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Antifungal Activity and Biochemical Characterization of Lectin Isolated from Locular Fluid of Cherry Tomato Fruit (방울토마토 열매 locular fluid lectin의 항균성과 생화학적 특성)

  • Roh, Kwang-Soo
    • KSBB Journal
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    • v.25 no.3
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    • pp.289-296
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    • 2010
  • Lectins are carbohydrate-binding and a cell-agglutinating proteins, and are concerted with a plants defence mechanism. In particular, chitin-binding lectins in locular fluid of cherry tomato fruit seemed to have a role in defending plants against fungi. The antifungal activity using lectin isolated from locular fluid of cherry tomato fruit was measured in the plant pathogen Cladosporium cucumerinum, Monosporascus cannonballus, Fusarium oxysporum, and Rhizoctonia solani. Amoung the four strains, a potent antifungal activity was detected in Cladosporium cucumerinum and Monosporascus cannonballus, not in Fusarium oxysporum, and Rhizoctonia solani. The molecular weight of this lectin isolated as double protein bands by SDS-PAGE was calculated to be 87 kDa and 47 kDa from the relative mobilities compared with those of reference molecular weight markers. The isolated lectin agglutinated human red blood cells (A, B, AB, O) treated with trypsin, and the most activity was found at B. The optimal temperature of isolated lectin was at $30^{\circ}C$. For the thermal stability, lectin was stable at $20-80^{\circ}C$. The optimal pH of this lectin was at 7.2, and showed complete loss below pH 9.0.

Adsorption Characteristics on Organic Solvents Diluted in Supercritical Carbondioxide Measured by Chromatography and IR Spectroscopy (적외분광법과 크로마토 측정기법을 이용한 초임계 이산화탄소 중에 희석시킨 유기용매의 흡착특성)

  • Jin, Do-Won;Kim, Young-Il;Park, Dong-Won
    • Applied Chemistry for Engineering
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    • v.9 no.1
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    • pp.76-81
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    • 1998
  • Physical adsorption on a silica gel(pore size of 80 nm, particle size of $10{\mu}m$)has been studied for binary mixture of acetone diluted in $CO_2$ by use of a FTIR transmission technique and we have compared the result of FTIR transmission technique with that of a chromatographic technique. Measurements were made at 313.2 K and under pressures up to 15MPa. As the pressure increases from 0.1MPa, the IA(Integral Absorbance) of the hydrogen-bonded OH groups interacting with acetone and adsorbed amount by use of a chromatographic technique increases at first, and reaches a maximum at a pressure below the critical pressure of $CO_2$, and then the intensities decrease gradually with increasing pressure. It is found that the pressure dependency of the chromatographic isotherm is a little larger than that of spectroscopic isotherm in the supercritical fluid region. This difference might be attributable to the weaker van der Waals force and relatively stronger hydrogen-bonding force influencing the adsorption of acetone on the sllica gel. The unique spectroscopic characteristics of amine group which vibrational frequencies of hydroxyl groups on the silica gel surface shift downward to about $1300cm^{-1}$ were measured from experimental result of triethylamine diluted in $CO_2$ or $N_2$.

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Phylogenetic Relationships and Genetic Diversity in Collected Resources of Carthamus tinctorius by Random Amplified Polymorphic DNA Markers (RAPD 마커에 의한 수집된 홍화자원에서 계통관계와 유전적 다양성)

  • Sung, Jung-Sook;Cho, Gyu-Taek;Lee, Gi-An;Baek, Hyung-Jin;Huh, Man-Kyu
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1764-1771
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    • 2010
  • Carthamus tinctorius L. (Compositae) is an herb primarily distributed throughout in the world. The species is regarded as ecologically important in the world. Safflower was used for medicines, as well as making red (carthamin) and yellow dyes. We have used the RAPD technique to investigate the phylogenetic relationships and genetic diversity of C. tinctorius. We obtained 123 bands from all the 26 cultivars. The average number of bands was 9.5 per primer. The genetic diversity of safflower is found among cultivars and there is a high among-cultivar differentiation. The OPC18-01 band is the specific marker for Syria cultivar, whereas no products were detected in individuals from other country cultivars. We found seven phenetic bands for determining the specific marker of cultivars with SCAR markers. Though the number of individuals sampled for analysis was small and probably not fully representative of the total available diversity in C. tinctorius, this study demonstrates that the regions (Morocco, Syria, and Turkey) of the Mediterranean Sea were more variable than other regions with the exception of India. In this result, although only simple result of RAPD is difficult to assert the center of species diversity of C. tinctorius, the regions of the Mediterranean Sea may be the most probable candidate for the origin of safflower. India was also the candidate of the center or secondary center of species diversity of C. tinctorius. RAPD markers were effective in classifying cultivar levels of safflower.

Studies on the Effects of Ozone Gas in Paddy Rice 3. Biochemical effects of ozone gas on rice plant (수도생육(水稻生育)에 대(對)한 Ozone가스의 영향(影響)에 관(關)한 연구(硏究) 3. Ozone가스에 의(依)한 수도엽의(水稻葉) 생화학적(生化學的) 변화(變化))

  • Kim, Bok-Young;Cho, Jae-Kyu;Lee, Suk-Hee
    • Korean Journal of Environmental Agriculture
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    • v.5 no.2
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    • pp.106-112
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    • 1986
  • Biochemical and histological effects of ozone gas (0.3 ppm) on rice plant were discussed. After ozone expoure, damage symptom, percentages of destroyed leaves, activities of peroxidase and polyphenoloxidase, and the contents of flavonoid, protein and sugar were examined on two rice varieties (Seokwangbyeo, Jinjubyeo), on tillering stage, and at different exposure time (0, 1, 3, 5 hr). The result were as followed. 1. The ozone-injured cell adjoining stomata become pigmented red-brown. 2. The percentage of injured leaves in Jinjubyeo was higher than that in Seokwangbyeo. 3. The activities of peroxidase and polyphenoloxidase increased on ozone-injured leaves. 4. The peroxidase activity increased with time in Jinjubyeo compared to Seokwangbyeo. 5. Peroxidase isozyme spectrum was altered after ozone exposure. 6. The content of flavonoid and reducing sugar in the rice leaves was increased, but the contents of protein was reduced.

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Growth and Optical Properties of PbSnSe Epilayers Grown on BaF2(111) (PbSnSe 단결정 박막의 성장과 광학적 특성)

  • Lee, Il-Hoon
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.1
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    • pp.35-41
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    • 2004
  • This study investigated the crystal growth, crystalline structure and the basic optical properties of $PbSnSe/BaF_2$ epilayers. The PbSnSe epilayer was grown on $BaF_2$(111) insulating substrates using a hot wall epitaxy (HWE) technique. It was found from the analysis of X-ray diffraction patterns that $PbSnSe/BaF_2$ epilayer was grown single crystal with a rock-salt structure oriented along [111] the growth direction. Using Rutherford back scattering, the atomic ratios of the PbSnSe was found to be proper stoichiometric. The best values for the full width at half maximum (FWHM) of the DCXRD was 162 arcsec for PbSnSe epilayer. The epilayer-thickness dependence of the FWHM of the DCXRD shows that the quality of the $PbSnSe/BaF_2$ is as expected. The dielectric function ${\varepsilon}(E)$ of a semiconductor is closely related to its electronic energy band structure and such relation can be drawn from features around the critical points(CPs) in the optical spectra. The real and imaginary parts(${\varepsilon}1$ and ${\varepsilon}2$) of the dielectric function ${\varepsilon}$ of PbSe were measured, and the observed spectra reveal distinct structures at energies of the E1, E2 and E3 CPs. These data are analyzed using a theoretical model known as the model dielectric function (MDF). The optical constants related to dielectric function such as the complex refractive index ($n^*=n+ik$), absorption coefficient (${\alpha}$) and normal-incidence reflectivity (R) are also presented for $PbSnSe/BaF_2$.

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Discussion of Preliminary Design Review for MIRIS, the Main Payload of STSAT-3

  • Han, Won-Yong;Jin, Ho;Park, Jang-Hyun;Nam, Uk-Won;Yuk, In-Soo;Lee, Sung-Ho;Park, Young-Sik;Park, Sung-Jun;Lee, Dae-Hee;Ree, Chang-H.;Jeong, Woong-Seob;Moon, Bong-Kon;Cha, Sang-Mok;Cho, Seoung-Hyun;Rhee, Seung-Woo;Park, Jong-Oh;Lee, Seung-Heon;Lee, Hyung-Mok;Matsumoto, Toshio
    • Bulletin of the Korean Space Science Society
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    • 2008.10a
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    • pp.27.1-27.1
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    • 2008
  • KASI (Korea Astronomy and Space Science Institute) is developing a compact wide-field survey space telescope system, MIRIS (The Multi-purpose IR Imaging System) to be launched in 2010 as the main payload of the Korea Science and Technology Satellite 3. Through recent System Design Review (SDR) and Preliminary Design Review (PDR), most of the system design concept was reviewed and confirmed. The near IR imaging system adopted short F/2 optics for wide field low resolution observation at wavelength band 0.9~2.0 um minimizing the effect of attitude control system. The mechanical system is composed of a cover, baffle, optics, and detector system using a $256\times256$ Teledyne PICNIC FPA providing a $3.67\times3.67$ degree field of view with a pixel scale of 51.6 arcsec. We designed a support system to minimize heat transfer with Muti-Layer Insulation. The electronics of the MIRIS system is composed of 7 boards including DSP, control, SCIF. Particular attention is being paid to develop mission operation scenario for space observation to minimize IR background radiation from the Earth and Sun. The scientific purpose of MIRIS is to survey the Galactic plane in the emission line of Pa$\alpha$ ($1.88{\mu}m$) and to detect the cosmic infrared background (CIB) radiation. The CIB is being suspected to be originated from the first generation stars of the Universe and we will test this hypothesis by comparing the fluctuations in I (0.9~1.2 um) and H (1.2~2.0 um) bands to search the red shifted Lyman cutoff signature.

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in vitro Anticancer Activity and in vivo Chronic Toxicity of Homoharringtonine (개비자나무 유효성분인 homoharringtonine의 in vitro 항암활성 및 in vivo 만성 독성)

  • Yoo, Gui-Jae;Cho, Chul-Hee;Lee, Gun-Soon;Ryoo, Zae-Young;Chae, Hee-Jeong
    • Applied Biological Chemistry
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    • v.51 no.2
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    • pp.124-128
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    • 2008
  • Cell proliferation inhibitory effects of homoharringtonine (HHT), an active drug substance in Cephalotaxus koreana, against blood cancer cell line K562 were evaluated. In addition, in vivo chronic toxicity test with mouse was carried out. When K562 cell line was treated everyday for 9, 6, 3 days, $IC_{50}$ values of HHT were determined as 0.27, 0.37, and 1.10 mM respectively. The anticancer activity of HHT was comparable to adriamycin, a known anticancer drug compound for blood cancer treatment. in vivo chronic toxicity test of the HHT, the number of red blood cell (RBC) showed no significant difference. From the analysis of the liver-functional enzymes in blood, all of liver damage related enzymes such as glutamate-oxalate-transferase (GOT), glutamate-pyruvate-transferase (GPT), cholesterol (Chol) and alkaline phosphatase (ALP) showed no significant change. However, from the histologic test, a neutrophil of the band type in liver tissue was observed.

Relationship Between Color Characteristic and Reflectance Index by Ground-based Remote Sensor for Tobacco Leaves (연초 엽의 색 특성과 원격탐사 반사율지표의 상호관계)

  • Hong, Soon-Dal;Kang, Seong-Soo;Jeon, Sang-Ho;Jeong, Hyun-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.4
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    • pp.274-279
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    • 2009
  • To determine the critical level for optimum maturity of flue-cured tobacco leaves (KF118) at the stalk position from cutter to tips, the reflectance index using ground-based remote sensors and chlorophyll meter were investigated. The sensors estimated were Crop $Circle^{TM}$ (Holland Scientific), Green $Seeker^{TM}$ (Ntech Industries), Spectroradiometer (LICOR, LI-1800), Chlorophyll meter (SPAD502, Minolta), and Field $Scout^{TM}$ Chlorophyll meter (CM-1000, Spectrum). The L, a, b values and greenness for flue-cured leaf were measured and estimated for correlation with sensor's measurement of harvested leaf. On a reflectance curve of 340nm~1100 nm, the reflectance peaks on 550nm and 675 nm for the harvested leaf were lowered as change from light green to darker green. Darker green leaf harvested produced darker flue-cured leaf. The reflectance at 675 nm for flue-cured leaf decreased as greenness increased in the harvested leaf. This result means that the red edge band of 675 nm wavelength is related to the absorbance of chlorophyll for photosynthesis. The greenness of flue-cured leaf showed significantly positive correlation with the entire reflectance indexes for harvested leaf while the L value by colorimeter showed negative correlation with greenness of cured leaf. The critical level for optimum maturity of harvested leaf were less than 22, 135, and 0.43 for SPAD reading, CM-1000 reading, and gNDVI by Crop $Circle^{TM}$, respectively. Consequently, ground-based remote sensing providing a non-destructive real-time assessment of plant greenness could be a useful tool in the selection of optimum maturity of flue-cured tobacco leaves in relation to high quality of flue-cured tobacco.

Simple Semiquantitative Determination and Selective Preconcentration of Trace Heavy Metals in Environmental Pollutants : Determination of Chromium (VI) with DPC Gel (環境汚染 重金屬의 選擇的 濃縮 및 簡易分析法 : DPC 겔의 의한 크롬 (VI) 의 定量)

  • Yong Keun Lee;Kyu Ja Whang;In Hwa Woo
    • Journal of the Korean Chemical Society
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    • v.25 no.4
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    • pp.275-282
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    • 1981
  • A simple semiquantitative procedure was developed for the determination of sub-ppm level of chromium(VI) in aquatic samples by using an analytical micro-column packed with diphenylcarbazide(DPC) gel beads. DPC gel beads were prepared by swelling XAD-2 resin(115∼150 mesh in dry condition) in ethanol for 10min, packing into a glass column(1.5 mm bore, 65nm length) and adsorbing 1ml of ethanol solution of $2{\times}10^{-3}M$ DPC for 20min at room temperature. When 0.5ml of ethanol solution containing chromium(VI) was passed through the DPC gel column for 40min, the original white color of the reagent gel turned to red-violet from the up-stream of the column. As the length of colored band was proportional to the total amount of chromium(VI) in the sample solution passed through the column, the concentration of chromium(VI) could be determined from the calibration line which had been prepared by using the standard solution. Chromium(VI) ion as small as from 0.1 to 0.8 ppm could be determined with ${\pm}5{\sim}{\pm}15{\%}$ relative errors. Since other interfering cations were few, 100-fold excess of Fe(III), 50-fold excess of Cu(II) could be masked with EDTA. This method was successfully applied to the analysis of chromium(VI) in industrial effluents.

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An Analysis of 2007 Summer Women's Street Fashion in Dalian, China (2007년 여름 중국(中國) 대련(大連)시 여성 스트리트 패션 분석)

  • Bae, Soo-Jeong;Baek, Jeong-Hyun;Choi, Yoon-Kyu;Yoo, Wha-Jung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.11
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    • pp.1692-1703
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    • 2008
  • On 2008, China comes to be an era of opening the market for 30 years, being both the 4th economically biggest country and 2nd ranked clothing export country in the world. China plays a huge consuming market for the world, along with the competition arena for the numerous world fashion brands while more than 100 kinds of Korean fashion ones being launched into the China. By applying the analysis and investigation of preferred street style. in local area to the development of fashion items and design, the synergistic effect of university and industrial corporation might also be expected. This study aims to provide the basic resources for the development of fashion design on behalf of the Korean fashion brands launched into China and moreover to anticipate the requirement on the part of Chinese consumers by way of understanding the preferred fashion styles, through analysis and investigation of the street fashion in Dalian, northeastern area of China. Conclusively, in terms of clothing style, 80.5% of casual style and 19.5% of formal style were notified. The jean casual, and easy casual being dominant in the former, feminine style in the later. In view of the high frequency of T-shirt, sleeveless on the top, full-length pants and 7/9 length pants on the bottom. The simple and cool style seems to be favored, making them easy through the hot summer. The upper clothes exposed in body part of back, chest and shoulder, were frequently shown. In light of colours. white, black, red were preferred on top, blue and black were preferred on bottom. To deal with accessories, the shoulder bag mostly preferred among bags, the sandal, slippers, snickers in sequential order among shoes, the hair pins and rubber band(tied or, held up style), in hair accessories. Other items of hat, belt, and sunglasses, which could give a point to the summer style, were identified low frequency.