• Journal of the Korean Society of Food Science and Nutrition
• /
• v.30 no.2
• /
• pp.222-227
• /
• 2001

The members of the genus Vibrio include harmless aquatic strain as well as strains capable of causing infections in human and fish. Pathogenic mechanisms are only understood for Vibrio cholerae O1 and O139 and not for the majority of Vibrio species. Twelve clinical and nonclinical strains were examined by in vitro and in vivo experiments for the importance of extracellular enzymes as a virulence determinant of Vibrio species. In vivo cytotoxicity assay was performed by injecting approximately $10^{8}$ cells/mL into mice (BALB/c). V. harvyi and V. vulnificus showed 100% lethality within 3hr after bacterial injection. V. fluvialis and four strains of V. parahaemolyticus showed 50% lethality within 4hr. V. mimicus, V. alginolyticus and V. furnissii revealed 30% lethality within 9hr. Nonclinical strains, V. campbellii and V. ordalii, did not show any lethality. In vitro protease and hemolytic activities were also good indicators for clinical and nonclinical strains of Vibrio species. The clinical strains showed much higher activities than nonclinical strains. The activity of some clinical strains of re-isolates was evidently increased. Most clinical strains had $\beta$ hemolytic activity. The results demonstrate that the prevalent distribution of extracellular proteases in pathogenic Vibrio sp. implies their importance as a virulence determinant.

• Research in Plant Disease
• /
• v.19 no.1
• /
• pp.49-54
• /
• 2013

Sclerotinia white rot disease was observed on 5 and 6-year-old ginseng (Panax ginseng) roots in Hongchun, Cheorwon, and Yanggu, Gangwon Province, Korea from 2006 to 2010. Symptoms included a brownish watery soft rot of the roots, and black sclerotia were often found on the rotten roots. The causal agent of the disease was identified as Sclerotinia nivalis based on cultural characteristics and sequence analyses of the internal transcribed spacer region of rDNA and ${\beta}$-tubulin gene with 100% sequence similarity. Pathogenicity tests were performed on 2-year-old ginseng roots with mycelium plugs without wounds. A watery soft rot of the roots and black sclerotia were observed 10 days after inoculation. These symptoms were identical to those observed on naturally infected roots. The same fungus was re-isolated from the lesions induced by artificial inoculation. This is the first report of sclerotinia white rot caused by S. nivalis on P. ginseng in Korea.

• Journal of Food Hygiene and Safety
• /
• v.14 no.1
• /
• pp.122-128
• /
• 1999

The cholesterol-lowering effect of a fermented milk was studied for long-term period (56 weeks) in 206 Korean adults (106 normal cholesterol group and 100 hypercholesterol group) 300 $m\ell$ of the fermented milk was taken daily to all subjects for 10 weeks. 24 subjects drinking the fermented milk for 56 weeks and 42 subjects no-drinking for 40 weeks and then re-drinking from 50 th week for 6 weeks were followed up. Paired t-test, Duncan's multiple range test, and GLM repeated measure were used for statistical analysis. The level of total blood cholesterol and LDL after drinking the fermented milk for 10 weeks were decreased significantly in both group and affected by total cholesterol level at baseline, BMI, smoking and drinking. there is no significant change in triglyceride, HDL, and LDL/HDL ratio by drinking the fermented milk. The fermented milk intake for long-term period (56 weeks) did not show any more effect after 10 week on the level of TC, TG, HDL, LDL.

• Journal of the Society of Naval Architects of Korea
• /
• v.48 no.3
• /
• pp.260-266
• /
• 2011

For long slender offshore structures, such as cables and pipe lines, their interaction with surrounding fluid flow becomes an important issue for global design of ocean systems. We employ a long circular cylinder as a representative case of slender offshore structure. A flexibly mounted cylinder in cross-flow generates complex vortex shedding and results in oscillation of the structure. In this paper, flow behind a circular cylinder at Re=100 is simulated. The vortex shedding pattern and flow induced motion are examined in the cross flow configuration as well as with various yaw-angled configurations. The "Lock-in" phenomenon is also observed when reduced velocity is approximately 4.0. The MAC Grid system, which is the typical grid system for Cartesian mesh and pressure correction methods, are used for solving the incompressible Navier-Stokes equations. Predictor/Corrector method is applied for obtaining a non-linear response of structure at the flexibly mounted. The existance and motion of the body is represented by the immersed boundary technique.

• The Plant Pathology Journal
• /
• v.31 no.1
• /
• pp.41-49
• /
• 2015

Sweet cherry is an important fruit crop with increasing economical value in Turkey and the world. A number of viruses cause diseases and economical losses in sweet cherry. Prune dwarf virus (PDV), is one of the most common viruses of stone fruits including sweet cherry in the world. In this study, PDV was detected from 316 of 521 sweet cherry samples collected from 142 orchards in 10 districts of Isparta province of Turkey by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). The presence of PDV in ELISA positive samples was confirmed in 37 isolates by reverse transcription- polymerase chain reaction (RT-PCR) method. A genomic region of 862 bp containing the coat protein (CP) gene of PDV was re-amplified from 21 selected isolates by RT-PCR. Amplified DNA fragments of these isolates were purified and sequenced for molecular characterization and determining genetic diversity of PDV. Sequence comparisons showed 84-99% to 81-100% sequence identity at nucleotide and amino acid level, respectively, of the CP genes of PDV isolates from Isparta and other parts of the world. Phylogenetic analyses of the CP genes of PDV isolates from different geographical origins and diverse hosts revealed that PDV isolates formed different phylogenetic groups. While isolates were not grouped solely based on their geographical origins or hosts, some association between phylogenetic groups and geographical origins or hosts were observed.

• The Korean Journal of Mycology
• /
• v.16 no.3
• /
• pp.157-161
• /
• 1988

The vegetative nuclear divisions in hyphae and chromosome numbers were studied with the aid of Giemsa-HCl techniques from 10 strains of Fusarium oxysporum. The entire nuclear division process occurred within an intact nuclear envelope like other fungus. The results confirmed that 2 strains(F. oxysporum S Hongchun D2, F. oxysporum S Jinyang 4) were n=4; 3 strains(F. oxysporum f. sp. lini KFCC 32585, F. oxysporum f. sp. melongenae KFCC 34743 and F. oxysporum f. sp. raphani) n=5; 2 strains(F. oxysporum f. sp. vasinfectum, and F. oxysporum f. sp. mori KFCC 34742) n=6; 3 strains(F. oxysporum f. sp. cucumerium, F. oxysporum f. sp.niveum, and F. oxysporum f. sp. pisi) n=7.

• Journal of Home Health Care Nursing
• /
• v.19 no.1
• /
• pp.11-18
• /
• 2012

Purpose: The aim of this study is to evaluate the efficiency of the outpatient clinics in a hospital, using DEA. Methods: Data were collected using an activity-based costing system, medical information system, and annual reports of customer satisfaction management team of a general hospital in a city. The input variables were the number of doctors, the number of nurses, and the number of staffs. The output variables were the number of treatment times, the number of outpatient clinic patients, the total profits from outpatient clinic, the patient's satisfaction score, and the number of re-visit appointments. EMS Window version 3.1 was used to measure the efficiency score and benchmarking analysis. Results: The average efficiency score of 24 outpatient clinics was about 82.01%. Thirteen outpatient clinics had 100% efficiency score among them. The lowest efficiency score was 57.56%. Conclusion: According to these results, we found that, generally, outpatient clinics were operated very efficiently. However, some outpatient clinics had low efficiency and they needed specialized outcome improvement strategies. To increase the efficiency of inefficient outpatient clinics, we will recommend using results of DEA, as a benchmark point of the most efficient outpatient clinics.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2003.05a
• /
• pp.31-34
• /
• 2003

Sandwich panel is composed of the facing sheets which support the external load, the cellular core with the low thermal conductivity and the adhesive agent to bond them. The cellular core was produced by binding lightweight cellular aggregates with cement and two types of acrylic base St/BA emulsion were added with a view to improving the workability ion due to high absorption of light weight aggregate and to develope more strength, respectively. This investigation is to comprehend the effect of the addition of two types of St/BA on thermal conductivity, calorific value and exhaustion content of noxious gas in addition re compressive and flexural strength. Flexural strength of the specimen made with St/BA-2 ranged 20kgf/cm2 to 25kgf/cm2 and was about 50% to 100% as high as that of the non-fiber specimen. Thermal conductivity was recorded from 2.0 to 3.0 kcal/mh$^{\circ}C$ and calorific value of St/BA modified specimen was much lower than that of commercial sandwich panel core of EPS and urethane. Careful caution has to be taken because generation of noxious gas such as CO, NO and SO2 tend to increase with addition of polymer cement ratio.

• Proceedings of the KIEE Conference
• /
• 1987.11a
• /
• pp.504-507
• /
• 1987

Interpenerating Polymer Networks (IPNs) are unique type of polymer blend, synthesized by swelling a crossed polymer (Epoxy) with second polymer (Silicon) and also we adopted Urethane as the second polymer. The relationship between dielectric and mechanical properties of high temperature curing IPNs(E/S, E/U) are investigated. The ratios of weight that we formed we re two kind of thing, one (E/S) about 1[wt%]. 3[wt%], 5[wt%], 7[wt%], 10[wt%], and the other (E/U) about 5[wt%], 15[wt%], 25[wt%]. It was heat-cured for 24hours at $100^{\circ}C$ 48 hours at $10^{\circ}C$, 5 hours at $130^{\circ}C$, 15hours at $130^{\circ}C$ in E/S and also for 5 hours at $130^{\circ}C$ in E/U. From the viewpoint of dielectric and mechanical properties, the optimum condition is obtained from the sample cured for 5hours at $130^{\circ}C$ for 1[wt%] in the E/S, and also obtained from the sample cure d for 5 hours at $130^{\circ}C$ in E/U.

• Journal of the Society of Naval Architects of Korea
• /
• v.49 no.5
• /
• pp.410-415
• /
• 2012

An experimental study is carried out to investigate the near-wake characteristics of a NACA 0018 foil with a flat plate. Two-frame grey-level cross correlation PIV method is used to measure the local flow characteristic around a pitch damping foil to control the vertical motion of high speed crafts in a circulating water channel. The analysis also includes angles of attack 10 and 20 degrees respectively. Reynolds number $Re{\fallingdotseq}3.5{\times}10^4$ based on the chord length(C=100mm) of NACA0018 has been applied during the whole experiments. The distance between the foil and the flat plate is D/C=0.5, 1.0 and 1.5 respectively. The channel effect according as the distance between the foil and the flat plate has a close relation with the velocity distributions around the foil. In the wake of 20-degree of attack, the complex turbulent flow and a thick boundary layer are formed due to the processes of vortex generation and dissipation.