• Title/Summary/Keyword: R.L.I.

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Pressure Drop of Integrated Hybrid System and Microbe-population Distribution of Biofilter-media (통합 하이브리드시스템의 압력강하 거동 및 바이오필터 담체의 미생물 population 분포)

  • Lee, Eun Ju;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.60 no.1
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    • pp.116-124
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    • 2022
  • In this study, waste air containing ethanol and hydrogen sulfide, was treated by an integrated hybrid system composed of two alternatively-operating UV/photocatalytic reactor-process and biofilter processes of a biofilter system having two units with an improved design (R reactor) and a conventional biofilter (L reactor). Both a pressure drop (△p) per unit process of the integrated hybrid system and a microbe-population-distribution of each biofilter process were observed. The △p of the UV/photocatalytic reactor process turned out very negligible. The △p of the L reactor was observed to increase continuously to 4.0~5.0 mmH2O (i.e., 5.0~6.25 mmH2O/m). In case of R reactor, its △p showed the one below ca. 16~20% of the △p of the L reactor. Adopting such microbes-carrying biofilter media with high porosity as waste-tire crumb media, and the improved biofilter design, contributed to △p of this study, reduced by ca. 37~50% and 40~53%, respectively, from the reported △p of conventional biofilter packed with biofilter media of the mixture (50:50) of wood chip and wood bark. In addition, the △p of R reactor in this study, reduced by ca. 80% from the reported △p of conventional biofilter packed with biofilter media of the mixture (75:25) of scoria with high porosity and compost, was mainly attributed to adopting the improved biofilter design. On the other hand, in case of L reactor, the CFU counts in its lowest column was analyzed double as much as those in any other columns. However, in case of R reactor, its CFU counts were bigger by 50% than the one of L reactor and its microbes were evenly distributed at its higher and lower columns of Rdn reactor and Rup reactor. This phenomena was attributed to an even moisture distribution of 50~55% of R reactor at its higher and lower columns. Therefore, R reactor showed superb characteristics in terms of both △p and microbe-population-distribution, compared to L reactor.

Construction of Plasmids for Overproduction of L-Phenylalanine (L-페닐알라닌 대량생산을 위한 재조합 플라스미드 구성)

  • Lee, Sae-Bae;Park, Chung;Won, Chan-Hee;Choi, Duk-Ho;Lim, Bun-San
    • Korean Journal of Microbiology
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    • v.28 no.2
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    • pp.169-173
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    • 1990
  • For the overproduction of L-phenylalanine using Escherichia coli, the authors constructed various recombinant plasmids including pMW 10, pMW 11 and pMW 12. The $aroF{FR}$ and $pheA^{FR}$ genes for the production of L-phenylalanine were isolated from Escherichia coli MWEC 101-5 strains. The productivity and atability of Escherichia coli regulatory mutants containing recombinant plasmids were investigated to evaluate the efficiency of the $aroF^{FR}$ and $pheA^{FR}$ genes. The MWEC 101-5/pMW 11 strain produced 24.3g/l of L-phenylalanine while its stability was 73.8 percent. The specific activity of prephenate dehydratase in the MWEC 101-5/pMW 11 strain increased by 26-fold compared with that of Escherichia coli K-12.

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Role of LuxIR Homologue AnoIR in Acinetobacter nosocomialis and the Effect of Virstatin on the Expression of anoR Gene

  • Oh, Man Hwan;Choi, Chul Hee
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1390-1400
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    • 2015
  • Quorum sensing is a process of cell-to-cell communication in which bacteria produce autoinducers as signaling molecules to sense cell density and coordinate gene expression. In the present study, a LuxI-type synthase, AnoI, and a LuxR-type regulator, AnoR, were identified in Acinetobacter nosocomialis, an important nosocomial pathogen, by sequence analysis of the bacterial genome. We found that N-(3-hydroxy-dodecanoyl)- L -homoserine lactone (OH-dDHL) is a quorum-sensing signal in A. nosocomialis. The anoI gene deletion was responsible for the impairment in the production of OH-dDHL. The expression of anoI was almost abolished in the anoR mutant. These results indicate that AnoI is essential for the production of OH-dDHL in A. nosocomialis, and its expression is positively regulated by AnoR. Moreover, the anoR mutant exhibited deficiency in biofilm formation. In particular, motility of the anoR mutant was consistently and significantly abolished compared with that of the wild type. The deficiency in the biofilm formation and motility of the anoR mutant was significantly restored by a functional anoR, indicating that AnoR plays important roles in the biofilm formation and motility. Furthermore, the present study showed that virstatin exerts its effects on the reduction of biofilm formation and motility by inhibiting the expression of anoR. Consequently, the combined results suggest that AnoIR is a quorum-sensing system that plays important roles in the biofilm formation and motility of A. nosocomialis, and virstatin is an inhibitor of the expression of anoR.

Isolation and Characterization of Pseudomonas sp. KM10, a Cadmium- and Mercury-resistant, and Phenol-degrading Bacterium

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.388-398
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    • 1998
  • A bacterium which is resistant to both mercury and cadmium, and also capable of utilizing phenol as a carbon and energy source, was isolated from the Kumho River sediments near Kangchang Bridge, Taegu, Korea. The isolate was labeled Pseudomonas sp. KM10 and characterized. The bacteria grew in 4 mM $CdCl_2$and in $70{\mu}M$ $HgCl_2$. The bacteria efficiently removed over 90% of 1 g/l phenol within 30 h. In the presence of 1.250 g/l phenol, the growth of the microorganism was slightly retarded and the microorganism could not tolerate 1.5 g/l phenol. Curing of plasmid from the bacteria was carried out to generate a plasmidless strain. Subsequent experiments localized the genes for phenol degradation in plasmid and the genes for mercury resistance and cadmium resistance on the chromosome. Dot hybridization and Southern hybridization under low stringent conditions were performed to identify the DNA homology. These results showed significant homologies between the some sequence of the chromosome of Pseudomonas sp. KM10 and merR of Shigella flexneri R 100, and between the some sequence of the chromosome of Pseudomonas sp. KM10 and cadA of Staphylococcus aureus pI258. The mechanism of cadmium resistance was efflux, similar to that of S. aureus pI258 cadA, and the mechanism of mercury resistance was volatilization, similar to that of S. flexneri R100 mer.

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(weak) R-mingle: toward a fuzzy-relevance logic

  • Yang, Eun-Suk
    • Korean Journal of Logic
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    • v.10 no.2
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    • pp.125-146
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    • 2007
  • This paper investigates the relevance system R-mingle (RM) as a a fuzzy-relevance logic. It shows that RM is fuzzy in Cintula's sense, i.e., RM is complete with respect to linearly ordered L-matrices (or L-algebras). More exactly, we first introduce RM and its weak versions wwRM and wRM. We next provide algebraic and matrix completeness results for them.

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AN ALGEBRAIC STRUCTURE INDUCED BY A FUZZY BI-PARTIALLY ORDERED SPACE I

  • JU-MOK OH
    • Journal of Applied and Pure Mathematics
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    • v.5 no.5_6
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    • pp.347-362
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    • 2023
  • We introduce an algebraic structure induced by a fuzzy bipartial order on a complete residuated lattices with the double negative law. We undertake an investigation into the properties of fuzzy bi-partial orders, including their various characteristics and features. We demonstrate that the two families of l-stable and r-stable fuzzy sets can be regarded as complete lattices, and we establish that these two families are anti-isomorphic. Furthermore, we provide two examples related to them.

Crosslinking Ratio Analysis of Type I Atelocollagen (Type I Atelocollagen의 가교형성비 분석)

  • Ahn, Soo-Jin;Kim, Yo-Sook;Suh, Hwal
    • Journal of Biomedical Engineering Research
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    • v.17 no.4
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    • pp.479-490
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    • 1996
  • To utilize collagen as an implantable biomateriall the mcct widely used bovine skin origin Type I collagen was investigated Pepsin treated, Type I atelocollagen was extracted and crosslinked by the ultraviolet(W) ray with wavelength of 254nm or by various concentrations of glutaraldehyde to produce collagen membranes. The crosslink rates of the specimens were observed by a polarized light microscope, a scanning electron microscope, and a Fourier transform infrared (FT-lR) spectrometer. The followings are concluded 1. The collagen membranes produced by both 2.5% glutaraldehyde solution and 254nm UV ray irra- diation demonstrated similar morphologies on polarized light microscopic and scanning electron microscopic views. 2. The chemical structures of the crosslinked membranes by glutaraldehyde over 2.5% in concentrations revealed similar intensities to that of the UV ray irradiated one in FT-lR investigation. 3. To obtain optimal croulink in bovine stalin origin Type I atelocollagen, 2.5% glutaraldehyde solution or UV ray irradiation with 254nm wavelength is acceptable.

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LINEAR EDGE GEODETIC GRAPHS

  • Santhakumaran, A.P.;Jebaraj, T.;Ullas Chandran, S.V.
    • Journal of applied mathematics & informatics
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    • v.30 no.5_6
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    • pp.871-882
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    • 2012
  • For a connected graph G of order $n$, an ordered set $S=\{u_1,u_2,{\cdots},u_k\}$ of vertices in G is a linear edge geodetic set of G if for each edge $e=xy$ in G, there exists an index $i$, $1{\leq}i$ < $k$ such that e lie on a $u_i-u_{i+1}$ geodesic in G, and a linear edge geodetic set of minimum cardinality is the linear edge geodetic number $leg(G)$ of G. A graph G is called a linear edge geodetic graph if it has a linear edge geodetic set. The linear edge geodetic numbers of certain standard graphs are obtained. Let $g_l(G)$ and $eg(G)$ denote the linear geodetic number and the edge geodetic number, respectively of a graph G. For positive integers $r$, $d$ and $k{\geq}2$ with $r$ < $d{\leq}2r$, there exists a connected linear edge geodetic graph with rad $G=r$, diam $G=d$, and $g_l(G)=leg(G)=k$. It is shown that for each pair $a$, $b$ of integers with $3{\leq}a{\leq}b$, there is a connected linear edge geodetic graph G with $eg(G)=a$ and $leg(G)=b$.

Effect of Fruit Thinner on Fruit Set and Quality in 'Hongro' and 'Fuji' Apples (적과제 처리가 '홍로'와 '후지' 사과의 착과 및 과실품질에 미치는 영향)

  • Yoo, Jingi;Park, Moo-Yong;Kang, In-Kyu
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.577-583
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    • 2014
  • Benzyladenine (BA, 99% purity), MaxCel$^{(R)}$ (1.9% BA), Fruitone (3.5% NAA), MaxCel$^{(R)}$ + Fruitone, a nd s imazine were applied postbloom as fruitlet thinning agents to mature 'Hongro' and 'Fuji' apple (Malus domestica Borkh.) trees. BA and MaxCel$^{(R)}$ were applied at $100mg{\cdot}L^{-1}$ a.i. while Fruitone at $0.1mg{\cdot}L^{-1}$ a.i. and simazine at $400mg{\cdot}L^{-1}$ a.i. All PGRs were applied at 8 days after full bloom (DAFB, 6 mm fruit diameter) in both cultivars, while simazine was treated twice at 7 and 14 DAFB. In 'Hongro', the number of total fruit set per flower cluster in terminal buds was 1.67, 1.84, and 1.81 in MaxCel$^{(R)}$ + F ruitone, MaxCel$^{(R)}$, and simazine applications, respectively, when compared with 2.35 of water control. These reductions in fruit set were mainly attributed to the increased ratio of defruited clusters by the thinning agents. In 'Fuji' apple, the number of total fruit set per flower cluster in terminal buds was 1.29, 1.60, and 1.76 in MaxCel$^{(R)}$ + Fruitone, Fruitone, and MaxCel$^{(R)}$, respectively, when compared with 2.56 of water control in 'Fuji' apple. The addition of Fruitone to the MaxCel$^{(R)}$ promoted the thinning efficacy in both cultivars, compared to MaxCel$^{(R)}$ only. The thinning efficacies were similarly observed with lateral flowers in both cultivars. A significant increase of fruit weight by the postbloom thinning treatments was observed only in the BA application in 'Hongro', while the effect was observed in BA and MaxCel$^{(R)}$ in 'Fuji'. While the soluble solids content increased in the BA, MaxCel$^{(R)}$ and MaxCel$^{(R)}$+Fruitone treatments in both cultivars, other fruit quality attributes were not affected by the application of post-bloom thinning agents.

A Study on Mixed Mode I/II Interlaminar Fracture Toughness of Carbon Fiber Reinforced Plastic Composites (CFRP 복합재료의 혼합모드 I/II 층간파괴인성치에 관한 연구)

  • Kim, H.J.;Park, M.I.;Kim, J.D.;Koh, S.W.
    • Journal of Power System Engineering
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    • v.4 no.3
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    • pp.48-54
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    • 2000
  • This paper describes the effect of molding pressure, specimen geometries for Mixed Mode I/II interlaminar fracture toughness of carbon fiber reinforced plastic composites by using asymmetrical double cantilever beam(ADCB) specimen. The value of $G_{I/IIC}$ as a function of various molding pressure is almost same at 307, 431, 585 kPa. However it shows the highest value under 307 kPa molding pressure. The effect of $G_{I/IIC}$ due to the change of initial crack length of ADCB specimen was almost negligible in this study. It turns out that the condition for mix mode quasi-static crack growth in ADCB specimen is the ratio of the crack length to that of the specimen, i.e., ${\alpha}/L<0.4$.

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