Crosslinking Ratio Analysis of Type I Atelocollagen

Type I Atelocollagen의 가교형성비 분석

  • Ahn, Soo-Jin (Department of Biomedical Reaserch Center, Korea Institute of Science and Technology) ;
  • Kim, Yo-Sook (Department of Biology, College of Natural Science, Catholic University of Korea) ;
  • Suh, Hwal (Department of Medical Engineering, Yonsei University, College of Medicine)
  • 안수진 (한국과학기술연구원 의과학연구센터) ;
  • 김요숙 (가톨릭대학교 자연과학대학 생물학과) ;
  • 서활 (연세대학교 의과대학 의용공학 교실)
  • Published : 1996.12.01

Abstract

To utilize collagen as an implantable biomateriall the mcct widely used bovine skin origin Type I collagen was investigated Pepsin treated, Type I atelocollagen was extracted and crosslinked by the ultraviolet(W) ray with wavelength of 254nm or by various concentrations of glutaraldehyde to produce collagen membranes. The crosslink rates of the specimens were observed by a polarized light microscope, a scanning electron microscope, and a Fourier transform infrared (FT-lR) spectrometer. The followings are concluded 1. The collagen membranes produced by both 2.5% glutaraldehyde solution and 254nm UV ray irra- diation demonstrated similar morphologies on polarized light microscopic and scanning electron microscopic views. 2. The chemical structures of the crosslinked membranes by glutaraldehyde over 2.5% in concentrations revealed similar intensities to that of the UV ray irradiated one in FT-lR investigation. 3. To obtain optimal croulink in bovine stalin origin Type I atelocollagen, 2.5% glutaraldehyde solution or UV ray irradiation with 254nm wavelength is acceptable.

Keywords

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