• Title/Summary/Keyword: Purification plant

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Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes kirilowii Root

  • Yun, Doo-Hee;Park, Eun-Ju;Park, Jong-Ok;Lee, Young-Han;Seo, Jeong-Kon;Ryu, Sung-Ho;Suh, Pann-Ghill;Kim, Hee-Sook
    • BMB Reports
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    • v.28 no.1
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    • pp.6-11
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    • 1995
  • A new lectin, named TRA, was purified from Trichosanthes kirilowii root by acid-treated Sepharose 6B, Mono-Q, and TSK-gel 3000SW column sequential chromatography. The lectin appeared homogeneous by native gel electrophoresis at pH 4.3 and gave two protein bands of Mr=31 and 28 kDa by SDS-PAGE. The N-terminal amino acid sequences of the polypeptides of TRA have not been reported in amino acid sequences of the lectins. TRA lectin formed a precipitate with asialofetuin, neuraminidase-treated fetuin. A sugar inhibition assay indicated that N-acetyl-D-galactosamine, among the monosaccharides tested, was the most potent inhibitor of TRA-induced hemagglutination. Asialofetuin showed a 260-times stronger inhibitory activity than N-acetyl-D-galactosamine. TRA lectin also showed agglutination with normal leukocytes and lymphoma cells, but not with premature hemopoietic cells. These results suggest that TRA is a novel plant lectin.

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Inhibitory Activity of Chinese Medicinal Plants on Nitric Oxide Synthesis in Lipopolysaccharide -Activated Macrophages

  • Ryu, Jae-Ha;Ahn, Han-Na;Lee, Hwa-Jin;Feng, Li;Qun, Wen-He;Han, Yong-Nam;Han, Byung-Hoon
    • Biomolecules & Therapeutics
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    • v.9 no.3
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    • pp.183-187
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    • 2001
  • Nitric oxide (NO) produced in large amounts by the inducible nitric oxide synthase (iNOS) is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of Chinese medicinal plants and screened their inhibitory activity against NO production in lipopolysaccharide (LPS)-activated macrophages. Among the 80 kinds of extracts of herbal drugs, 15 extracts showed potent inhibitory activity of NO production above 80% at the concentration o$50\mu\textrm{g}/ml$. These potent extracts showed dose dependent inhibition of NO production of LPS-activated macrophages at the concentration of 50, 30,$10\mu\textrm{g}/ml$. Especially, Rhus chinensis, Senecio scandens and Wikstroemia indica showed most potent inhibition above 50% at the concentration of $10\mu\textrm{g}/ml$. These plants are promising candidates for the study of the activity-guided purification of active compounds and would be useful for the treatment of inflammatory diseases and endotoxemia accompanying the overproduction of NO.

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A NEW TREATMENT SYSTEM FOR ANIMAL WASTE WATER USING MICROORGANISM, SOIL AND VEGETATION

  • Oshida, T.;Fukuyasu, T.;Kohzaki, K.;Izumikawa, Y.;Kawanabe, S.;Konishi, S.;Oikawa, N.;Matsumoto, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.2
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    • pp.205-209
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    • 1993
  • A new treatment system for animal waste water has been developed as an alternative to the activated sludge process. It consists of two treatments; one is operated with 7 tanks, and the other is soil and plant cultivation bed. Aerobic microorganisms are added to the influent water in the tanks where the water is aerated so that the microbes utilize the pollutants, while sedimentation removes the indigestible solids. In the secondary treatment the water, which has already received a primary treatment, is filtered through soil where it also receives treatment by soil organisms. In addition there is transpiration of water and absorption of minerals by plants. In the primary treatment BOD, SS, coliforms (E. coli), TP and total bacteria were removed 79-99%, but COD and TN were removed only 58% and 36%, respectively. In the secondary treatment removal of nutrients proceeded further, and 93-99% of pollutants were removed. The treated waters met the quality standard of discharge water in Japan except for TN, which was in too great a concentration to meet discharge standards. This problem requires further study.

Performance of Backwashing Process in Biological Activated Carbon Column (생물활성탄접촉조에서 역세척 공정의 성능)

  • Lee, Gangchoon;Yoon, Taekyung;Moon, Byunghyun;Noh, ByeongIl
    • Journal of Korean Society on Water Environment
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    • v.22 no.6
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    • pp.1082-1087
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    • 2006
  • BAC backwashing process in ozone-BAC advanced water treatment process was experimentally studied. The operation and performance of backwashing were evaluated by measuring the effects of water temperature and water input rate on the backwashing interval and duration, and also the change of the amounts of biofilm and HPC in treated water before and after backwashing. The experiments were carried out with the pilot scale test module built in a existing water treatment plant, and the following results were obtained. Longer backwashing time than that of design operating condition was needed for satisfying the suitable turbidity of washing water effluent. Depending on water temperature, 7 days of backwashing cycle was recommended for the period lower than $15^{\circ}C$, and 10 days for the period higher than $15^{\circ}C$. After backwashing, the amounts of biofilm and HPC decreased to 1/10 and 80%, respectively.

Evaluation of the Water Purification Efficiency of Waste LCD Glass Media by Using Foaming Technology (발포기술을 이용한 폐 LCD유리 여재의 수질정화능력 평가)

  • Ahn, Tae-Woong;Choi, I-Song;Oh, Jong-Min
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.4
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    • pp.369-376
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    • 2010
  • The purpose of this study is to reprocess Waste-LCD(Liquid Crystal Display), to widely increase specific surface-area by foaming agent in the process of reprocessing and to use as a substrate of water treatment which is increased the ability of biological treatment, as well as to control non-point source pollutants produced by surface run off during rainfall with using this substrate, and to improve water quality of public watershed as developing substrate for water treatment to be able to purify second treated water which is exhausted at the wastewater treatment plant. The average removal efficiency of Waste-LCD that using the foaming technology was SS 71.2%, BOD 55.7%, COD 58.4%, T-N 29.5% and T-P was 50.3%. Almost Media, early stage showed low removal efficiency of SS and BOD. However, it became high when the microorganism adhered the Media. The variation of SS removal efficiency was high by inflow concentration of SS. The reason for the Media 4 showed high SS removal efficiency is that it has wide specific surface-area, and also it has a pore. All in all, it shows floating matter treatment ability not only inside but it also works outside of the substrate.

CFD ANALYSIS ON THE CHARACTERISTICS FOR FLOCCULATORS OF VERTICAL PADDLE AND HYDRO-FOIL TYPE (수직 패들형 및 하이드로 포일형 응집기 특성의 전산유체역학 해석)

  • Shin, J.H.;Chang, S.M.;Cho, Y.
    • Journal of computational fluids engineering
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    • v.21 no.3
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    • pp.24-30
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    • 2016
  • In the water purification plant, the mixture of water and chemical from the mixing basin enters the flocculation basin. The rotating flocculators are generally used for the efficient flocculation of dregs. In this paper, the performance of flocculators of a vertical paddle type, widely used in the typical flocculation basins, and a hydro-foil type, recently disseminated in the field, are compared with each other by use of the numerical method. Also the characteristics and the efficiency are analyzed with CFD techniques. The strain rate and the eddy viscosity are compared for two types to predict the mixing efficiency, and the maximum speed and its location are pursued from the computed data. The hydrofoil type shows that the eddy viscosity is enhanced 1.66 to 3.03 times larger than that of vertical paddle type, and also produced 1.87 to 1.95 times larger flocs for each stage. However, the rapid rotation of hydrofoil may chop the floc to small size due to the higher turbulence intensity. From the result of computation, the strong and weak points of each type have been analyzed for the decision making.

A Development of Water Supply Prediction Model in Purification Plant (정수장 생산량 예측모델 개발)

  • So, Byung-Jin;Kwon, Hyun-Han;Park, Rae-Gun;Choi, Byung-Kyu
    • Proceedings of the Korea Water Resources Association Conference
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    • 2011.05a
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    • pp.171-171
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    • 2011
  • 상수도의 합리적인 운용과 관리를 위해서는 급수량 예측이 매우 중요하다. 기존 급수량 예측은 신경망과 칼만 필터법을 사용한 연구들이 대부분이었다. 이러한 연구결과들은 높은 상관결과를 갖고 있지만 이는 자기상관계수에 대한 높은 의존도에 따른 결과로 볼 수 있다. 즉, 예측의 결과가 전날 수요량을 거의 그대로 따라오는 경향을 띄어, 급수량 예측 그래프가 기존 그래프를 오른쪽으로 이동시킨 것과 같이 나타난다. 본 연구에서는 이러한 문제점들을 해결하기 위해서 물수요량을 예측하는데 있어서 효과적인 예측인자를 도출하는 것이 우선되어야 할 것으로 판단되었다. 이에, 물수요량 특성을 효과적으로 나타내어 줄 수 있는 예측인자로서 강수량, 최저온도, 최고온도, 평균온도 등을 1차적으로 선정하였다. 이들 예측인자들과 서울시 물수요량과의 상관성을 평가하여 최적의 예측인자 Set과 지체시간 등을 산정하였다. 이렇게 선정된 예측인자와 Bayesian 통계기법 기반의 회귀분석 모형을 구축하여 물수요량을 예측하였다. 본 연구에서 적용하고자 하는 계층적 Bayesian 모형은 유사한 특성을 가지는 자료계열들 사이에서 서로 보완이 될 수 있는 정보들을 추출함으로써 모형이 갖는 불확실성을 상당히 줄일 수 있는 방법이다. 이러한 모형적 특징은 생산량 예측에 대한 불확실성 저감 측면에서 장점이 있을 것으로 판단된다. 본 연구에서는 광암, 암사, 구의, 뚝도, 영등포, 강북 정수장을 대상으로 모형의 적합성을 평가하였다. 이러한 연구결과는 향후 정수장 운영계획 및 동일한 시스템을 갖는 상수도 급수량 예측 시 유용하게 사용할 수 있을 것이다.

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Fungal-Sporulation Suppressing Substances Produced by Pseudomonas aeruginosa KMCS-1

  • Min, Bu-Yong;Shim, Jae-Young;Kim, Kun-Woo;Lee, Jong-Kyu;Choi, Hyung-Tae;Yoon, Kwon-Sang
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.284-288
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    • 1996
  • Among the bacteria isolated from compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 $\mu$g/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.

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Optimum Design of Animal Wastewater Treatment System (畜産廢水處理시스템의 最適說計要因 導出)

  • Oh, In-Hwan;Park, Joeng-Hyun;Kim, Beom-Seok;Lee, Sang-Rak;Maeng, Won-Jae
    • Journal of Animal Environmental Science
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    • v.1 no.1
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    • pp.47-53
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    • 1995
  • An efficient Treatment of animal wastewater is one of the hot issues for preventing the environmental pollution. It should be established the design parameter in order to purify the animal wastewater. A test is carried on in the pilot plant as a simplified activated sludge process. A vibration sieve separator is deviced to keep the pollution load constant by means of separation of solid matter. The BOD removal efficiency of the vibration sieve showed over 50%. As the test results, the BOD contents of the influent was in average of 3,000 mg/I and that of the effluent 85 mg/I. So, the BOD removal rate showed 97% in average. The SS-contents in the primary chamber was about 3,300 mg/I and that of effluent 92 mg/I. The SS removal efficiency showed 97%. The removal rate of total nitrogen and phosphore were in average of 82% respectively. Carrying out in winter season, it showed relatively good results; The design parameter approved in this test can be applied to the full-time farmers.

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Bacteria on Granular Activated Carbon for Tap Water Purifier (정수용 입상활성탄상의 세균)

  • 이동근;하종명;이재화
    • Journal of Life Science
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    • v.14 no.1
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    • pp.163-166
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    • 2004
  • To investigate the bacterial growth on granular activated carbon (GAC) for the purification of tap water, fixed bed columns with GAC were installed and operated at an empty bed contact time (EBCT) of 1 min$\pm$0.08 min. There was no bacterial breakthrough in the spring. However, the bacterial concentrations of effluent (($10^3$ CFU/ml) were higher than that of the influent ($10^2$ CFU/ml) after 10 day operation in summer. More bacteria were enumerated near the entering point of the tap water, while the bacterial activities were similar throughout the columns. Different bacterial species were detected on coal- and plant-based GAC, although the dominant genus was the same as Acinetobacter.