BMB Reports
- Volume 28 Issue 1
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- Pages.6-11
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- 1995
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- 1976-670X(eISSN)
Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes kirilowii Root
- Yun, Doo-Hee (Department of Food Science and Technology, Kyungsung University) ;
- Park, Eun-Ju (Department of Food Science and Technology, Kyungsung University) ;
- Park, Jong-Ok (Department of Chemistry, Kyungsung University) ;
- Lee, Young-Han (Department of Life Science, Pohang University of Science and Technology) ;
- Seo, Jeong-Kon (Department of Life Science, Pohang University of Science and Technology) ;
- Ryu, Sung-Ho (Department of Life Science, Pohang University of Science and Technology) ;
- Suh, Pann-Ghill (Department of Life Science, Pohang University of Science and Technology) ;
- Kim, Hee-Sook (Department of Food Science and Technology, Kyungsung University)
- Published : 1995.01.31
Abstract
A new lectin, named TRA, was purified from Trichosanthes kirilowii root by acid-treated Sepharose 6B, Mono-Q, and TSK-gel 3000SW column sequential chromatography. The lectin appeared homogeneous by native gel electrophoresis at pH 4.3 and gave two protein bands of Mr=31 and 28 kDa by SDS-PAGE. The N-terminal amino acid sequences of the polypeptides of TRA have not been reported in amino acid sequences of the lectins. TRA lectin formed a precipitate with asialofetuin, neuraminidase-treated fetuin. A sugar inhibition assay indicated that N-acetyl-D-galactosamine, among the monosaccharides tested, was the most potent inhibitor of TRA-induced hemagglutination. Asialofetuin showed a 260-times stronger inhibitory activity than N-acetyl-D-galactosamine. TRA lectin also showed agglutination with normal leukocytes and lymphoma cells, but not with premature hemopoietic cells. These results suggest that TRA is a novel plant lectin.