• Title/Summary/Keyword: Pull-through

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Construction of a Transgenic Plant to Develop a New Method for the Isolation of Calmodulin-Binding Proteins (새로운 방법을 이용한 칼모둘린 결합 단백질 분리를 위한 형질 전환 식물체의 구축)

  • Kim, Sun-Ho;Lee, Kyung-Hee;Kim, Kyung-Eun;Jung, Mi-Soon;Lim, Chae-Oh;Lee, Shin-Woo;Chung, Woo-Sik
    • Journal of Life Science
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    • v.17 no.9 s.89
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    • pp.1177-1181
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    • 2007
  • Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety CaM-binding proteins (CaMBPs). Because eukaryotes have multiple CaMBPs, it is important to isolate and characterize them in different tissues and conditions. So far a number of CaMBPs have been identified through classical screening methods. Many classes of proteins have been predicted to bind CaMs based on their structural homology with already known targets. In an effort to develop a method for large-scale analysis of CaMBPs in Arabidopsis, we have generated a transgenic plants overexpressing AtCaM2-GFP. We performed protein pull-down assay to test whether exogenously expressed AtCaM2-GFP proteins can interact with CaMBPs. The exogenously expressed AtCaM2-GFP could strongly interact with a CaMBP, AS1 protein. This result suggests that AtCaM2-GFP in transgenic plants may interact with many CaMBPs in plant cell. Therefore, we will be able to isolate kinds of CaMBPs by using these transgenic plants in many different tissue and environments.

Design of High-Power and High-Efficiency Broadband Amplifier Using 1:4 Transmission Line Transformer (1:4 전송 선로 트랜스포머를 이용한 고출력 고효율 광대역 전력 증폭기의 설계)

  • Kim, Kyung-Won;Seo, Min-Cheol;Cho, Jae-Yong;Yoo, Sung-Cheol;Kim, Min-Su;Kim, Hyung-Cheol;Oh, Jun-Hee;Sim, Jae-Woo;Yang, Youn-Goo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.21 no.2
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    • pp.121-128
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    • 2010
  • This paper presents a design of a 100 W high-efficiency power amplifier, whose operational frequency band expands from 30 to 512 MHz, using negative feedback network, push-pull structure, broadband RF choke, and transmission line transformer for balun configuration. The push-pull amplifier has been tuned for higher output power using a shunt capacitor as a matching component at its load especially for high-frequency region. The implemented power amplifier exhibited a very flat power gain of $18.34{\pm}0.9\;dB$ throughout the operating frequency band and very high power-added efficiency(PAE) of greater than 40% at an output power of 100 W. It also showed second- and third-harmonic distortion levels of below -34 dBc and -12 dBc, respectively, through the entire operating frequency band.

Design of a Stainless Steel Insert for Mechanical Joining of Long Fiber-reinforced Composite Structures (장섬유강화 복합재료 구조물의 기계적 접합을 위한 스테인레스 강 인서트 설계)

  • Lee, Sung-Woo;Chang, Seung-Hwan
    • Composites Research
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    • v.31 no.4
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    • pp.139-144
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    • 2018
  • Long Fiber-reinforced composites have advantages of excellent production efficiency and formability of complex shapes compared to conventional continuous fiber reinforced composite materials. However, if we need to make complicated composite shapes or to assemble parts made of different materials, a variety of joining methods are needed. In general, long fiber prepreg sheet (LFPS) contains mold release agent to facilitate demolding after thermoforming. Therefore, mechanical fastening is required in addition to the adhesive bonding to get proper joining strength. In this study, we proposed a stainless steel insert for co-cure bonding which cures LFPS and bonds the stainless steel insert through thermoforming process. The wing of the insert which is spread during the thermoforming process induces adhesion and mechanical wedging effect and serves as a hook to resist the pulling force. The burn-out method was used to confirm the unfolded state of the stainless steel insert wings inserted into the composite material. The static pull-out test was performed to quantitatively evaluate the joining strength. From these experimental results, the condition which guarantees the most appropriate joining strength was derived.

Pin Pull Characteristics of Pin Lead with Variation of Mechanical Properties of Pin Lead in PGA (Pin Grid Array) Package (PGA (Pin Grid Array) 패키지의 Lead Pin의 기계적 특성에 따른 Pin Pull 거동 특성 해석)

  • Cho, Seung-Hyun;Choi, Jin-Won;Park, Gyun-Myoung
    • Journal of the Microelectronics and Packaging Society
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    • v.17 no.1
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    • pp.9-17
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    • 2010
  • In this study, von Mises stress and total strain energy density characteristics of lead pin in PGA (Pin Grid Array) packages have been calculated by using the FEM (Finite Element Method). FEM computation is carried out with various heat treatment conditions of lead pin material under $20^{\circ}$ bending and 50 mm tension condition. Results show that von Mises stress locally concentrated on lead pin corners and interface between lead pin head and solder. von Mises stress and total strain energy density decrease as heat treatment temperature of lead pin increases. Also, round shaped corner of lead pin decreases both von Mises stress and total strain energy density on interface between lead pin head and solder. This means that PGA package reliability can be improved by changing the mechanical property of lead pin through heat treatment. This has been known that solder fatigue life decreases as total strain energy density of solder increases. Therefore, it is recommended that both optimized lead pin shape and optimized material property with high lead pin heat treatment temperature determine better PGA package reliability.

Bond Behavior of Epoxy Coated Reinforcement Using Direct Pull-out Test and Beam-End Test (직접인발시험과 보-단부 시험을 이용한 에폭시 도막 철근의 부착특성)

  • Kim, Jee-Sang;Kang, Won Hyeak
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.7 no.3
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    • pp.271-278
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    • 2019
  • The corrosion of reinforcements embedded in concrete causes severe deterioration in reinforced concrete structures. As a countermeasure, epoxy coated reinforcements are used to prevent corrosion of reinforcements. When epoxy coated bars are used, the resistance of corrosion is excellent, but epoxy coating on the bars have a disadvantage of reduction in bond capacity comparing to that of normal bars. Therefore, it is necessary to confirm the bond performance of epoxy coated reinforcements through experimental and analytical methods. Bond behaviors of epoxy coated bars for various diameters of 13 and 19mm and thicknesses of cover concrete of 3 types(ratio of cover to bar diameter) are examined. As the diameters of the epoxy coated bars increase, the difference of bond strength between epoxy coated and uncoated bars also increases and damage patterns showed pull out failure. In addition, finite element analysis was performed based on the bond-slip relationship obtained by direct pullout test and compared with the flexural test results. It is considered that flexural member test is more useful than pullout test for simulating the behavior of actual structure.

An Experimental Study on Concrete Bond Behavior According to Grid Spacing of CFRP Grid Reinforcement (격자형 CFRP 보강재의 격자간격에 따른 콘크리트 부착거동에 대한 실험적 연구)

  • Noh, Chi-Hoon;Jang, Nag-Seop;Oh, Hongseob
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.6
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    • pp.73-81
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    • 2022
  • Recently, as the service life of structures increased, the load-carrying capacity of deteriorated reinforced concrete, where corrosion of reinforcing bars occurs due to various causes, is frequently decreased. In order to address this problem, many studies on the bond characteristic of FRP (Fiber Reinforced Polymer) bars with corrosion resistance, light weight and high tensile strength have been conducted, however there are not many studies on the bond characteristic of grid-typed CFRP embedded in concrete. Therefore, in order to evaluate the bond characteristics of grid-typed CFRP and its usability as a substitute for steel rebar, a pull-out test is performed using the longitudinal bond length and transverse grid length of the grid-typed CFRP as variables. Through the pull-out test, the bond load-slip curve of the grid-typed CFRP is derived, and the bond behavior is analyzed. The total bond load equation is proposed as the sum of the bond force of the longitudinal bond length and the shear force of the grid in the transverse direction. Also, expressing the area of the bond load-slip curve as total work, the change in dissipated energy with respect to the slip is analyzed to examine the effect of the tranverse grid on the bond force.

The Study on the Effect of Cultural Difference on Overseas Travel Market: A Comparison among Korea, China, U.S. and Japan (문화차이가 해외여행 시장에 미치는 영향에 관한 연구: 한·중·미·일 비교를 중심으로)

  • Kim, Jonghyuk
    • International Commerce and Information Review
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    • v.19 no.1
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    • pp.213-234
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    • 2017
  • This study analyzed valid samples of 707 units collected by conducting paper and online surveys on the Korean, the Chinese, the American, and the Japanese. The result showed that a significant causal relationship exists between power distance and pull motivation as well as collectivism and push motivation, which led to a conclusion that developing travel packages that can strengthen bonding of fraternal societies through various events and attractions is effective for respondents from Asian countries. On the other hand, Americans turned out to prefer practical plans, which could provide individual's needs and preferences, for example, a self-healing package. This study, using a simple survey, may have a limitation in that it does not allow the participants to express their opinions. However, the study is meaningful that it made a theoretical contribution utilizing Hofstede's cultural dimensions index, two types of motivation, and theories of customer satisfaction and revisit intention. It also has a practical implication in that it proposes the most optimal and applicable overseas travel marketing strategy by comparing cultural traits of each country.

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A Study on the Preparation and Mechanical Properties of Hybrid Composites Reinforced Waste FRP and Urethane Foam (폐 FRP/Urethane Foam 충진 혼성복합재의 제조 및 기계적 물성에 관한 연구)

  • 황택성;신경섭;박진원
    • Polymer(Korea)
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    • v.24 no.4
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    • pp.564-570
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    • 2000
  • The waste FRP occured in the fabrication of SMC (sheet molding compound) bathtubs and the waste polyurethane foam occured in electronic manufacture and waste insulator were applied as a soundproof and light weight pannel in the waste FRP unsaturated polyester matrix resin composites to recycle. The effect of filler contents on the mechanical properties and interfacial phenomena of the filler and matrix on the composites was evaluated. The tensile strength of composites reached its maximum value of 82.34 MPa when the filler content was 70 wt%, and the more content of reinforcement is increased, the more tensile modulus was decreased. The flexural strength and modulus of composites, reinforced 70 wt% with filler content, were dominant compared to the other samples to 72.5 MPa, 958.4 MPa respectively. When composite of reinforced 70 wt% with filler content, it was confirmed that pull out phenomena and cracks did not occur in the interface of reinforcement and matrix resin through the SEM observation. Also, waste FRP and urethane foam were dispersed well into matrix resin as filler.

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Bond Strength Properties of Latex Modified Concrete (라텍스 개질 콘크리트의 부착강도 특성)

  • 윤경구;이주형;최상릉;김기헌
    • Journal of the Korea Concrete Institute
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    • v.13 no.5
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    • pp.507-515
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    • 2001
  • Significant improvements in bond strength between new and existing concrete can be achieved through the modification of the new concrete by latex. This study focuses on the investigation of bond strength of latex modified concrete. Pull-out bond test and uniaxial direct tensile bond test are adopted for evaluating the adhesion characteristics of latex modified concrete to conventional concrete substrate. The main experimental variables are test methods, latex-cement ratio, surface preparations and moisture levels. The results are as follows; The increase of latex-cement ratio substantially improves the adhesion between latex modified concrete and substrate. The effects of surface preparation at substrate into the bonding of latex modified concrete are quite different according to the conditions of surfaces. Thus, an adequate surface preparations are essential for good bond strength. Because the moisture level of the substrate may be critical to achieving bond, optimum moisture condition for a conventional concrete has evaluated in this study. The saturated condition of surface is the most appropriate moisture level among the considered, followed by dry condition and wet condition.

Design of High-Reliability Differential Paired eFuse OTP Memory for Power ICs (Power IC용 고신뢰성 Differential Paired eFuse OTP 메모리 설계)

  • Park, Young-Bae;Jin, Li-Yan;Choi, In-Hwa;Ha, Pan-Bong;Kim, Young-Hee
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.17 no.2
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    • pp.405-413
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    • 2013
  • In this paper, a high-reliability differential paired 24-bit eFuse OTP memory with program-verify-read mode for PMICs is designed. In the proposed program-verify-read mode, the eFuse OTP memory can do a sensing margin test with a variable pull-up load in consideration of programmed eFuse resistance variation and can output a comparison result through a PFb (pass fail bar) pin by comparing a programmed datum with its read one. It is verified by simulation results that the sensing resistance is lower with $4k{\Omega}$ in case of the designed differential paired eFuse OTP memory than $50k{\Omega}$ in case of its dual-port eFuse OTP memory.