• 한국식품영양학회지
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• 제23권4호
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• pp.516-525
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• 2010

아마인의 유용성분에 대해서 알아보고 가열과정을 통한 성분변화 및 다른 종유작물과 유지의 물리화학적 성분과 비교, 평가하였다. 조지방은 37~43%, 탄수화물은 30~35%, 조단백질은 18~23%, 조회분은 3~4% 그리고 수분 함량은 0.2~6.8%의 함량을 나타내었다. 식이섬유 함량은 생아마인보다 가열 처리한 볶음 아마인에서 SDF의 함량이 증가한 것을 알 수 있었다. 하지만 상대적으로 IDF의 함량도 감소하기 때문에 TDF의 함량은 큰 차이를 보이지 않았다. 그리고 총 식이섬유 함량은 28~31%로 높은 함량을 보였다. 또한 아마인의 주요 유용성분을 살펴본 결과, 비타민은 ${\gamma}$-tocopherol(E), 아미노산은 agrginine, aspartic acid, glutamic acid가 높은 함량을 보였다. 이들은 생아마인보다 볶음 아마인에서 낮은 함량을 나타내었다. 아마인의 무기물 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 인이 주요 구성성분이었다. 물리화학적인 특징으로 요오드가, 비누화가, 산가를 분석하였으며, 브라운 아마인유는 각각 204.1 g/100 g, 193.6 mg/g, 1.59 mg/g을 나타내었으며, 골드 아마인유는 각각 203.0 g/100 g, 189.9 mg/g, 2.35 mg/g으로 조사되었다. 아마인유의 지방산 함량에서는 전체적인 구성 비율을 볼 때 ${\alpha}$-linolenic acid(ALA, C18:3n-3)가 약 55.5~56.1% 로 가장 높은 함량을 차지하였다. 그리고 브라운보다는 골드 아마인유의 ${\alpha}$-linolenic acid의 함량이 약간 높았다. 높은 ${\alpha}$-linolenic acid 함량은 아마인유가 오메가-3 지방산이 강화된 식품 개발에 좋은 자원이 될 것으로 사료된다. 또한 다가 불포화지방산 함량이 약 71%로 함유되어 있으며, ${\alpha}$-linolenic acid 등 필수지방산의 함량이 높아 식품의 영양학적인 의의가 크며, 양질의 식용유로 사용 가능할 것이다.

• Applied Microscopy
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• 제30권4호
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• pp.377-387
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• 2000

뇌실을 감싸는 뇌실막층은 대부분이 일반적인 뇌실막 세포로 이루어졌으나, 이들 세포 사이에 간혹 띠뇌실막 세포(tanycyte)가 분포하고 있다. 띠뇌실막세포는 일반적인 뇌실막세포와는 달리 뇌의 실질로 뻗은 매우 긴 기저돌기를 갖고 있다. 특히 제3뇌실의 뇌실막층에서 주로 연구된 띠뇌실막세포는 뇌실과 뇌실질의 혈관 혹은 신경세포와의 사이에 물질교환을 담당하는 것으로 추측되고 있으며, 띠뇌실막세포는 일반적인 뇌실막세포와는 달리 glial fibrillary acidic protein(GFAP)항체에 대해 양성반응을 보이는 것으로 알려져 있다. 본 연구는 면역조직화학 및 면역금표지법을 이용하여 박쥐 맨아래구역을 감싸는 뇌실막층에서 GFAP 항체에 대해 양성반응을 보이는 세포의 분포여부 및 이의 미세 구조를 확인하고자 시행하여 다음과 같은 결론을 얻었다. 세로 절단한 절편을 대상으로 GFAP 항체를 이용하여 면역염색한 후 광학현미경으로 확인한 결과 맨아래구역을 감싸는 뇌실막층에 GFAP 양성반응을 보이는 세포가 관찰되었으며, 특히 맨아래구역의 목부분에서 양성반응 세포가 많이 모여 있었다. GFAP양성반응을 보이는 세포들은 매우 긴 기저돌기를 갖고 있었으며, 기저돌기에서도 매우 강한 GFAP 양성반응을 보였다. 세포체에서는 주변부에서 양성반응을 보였다. 전자현미경하에서 맨아래구역 띠뇌실막세포는 주로 ependymal tanycytes였으며, 자유면에 소수의 미세융모 및 세포질돌기가 관찰되었으나, 섬모는 관찰되지 않았다. 기저부에 특징적으로 긴 기저돌기를 갖고 있었으며 이 돌기에는 중간세사 및 세로로 길게 달리는 사립체가 발달되어 있었고, 세포체와 돌기에 지방방울이 산재되어 있었다. 금입자를 표지한 GFAP항체를 사용하여 면역 염색한 후 전자현미경으로 관찰한 결과 세포체에서 핵을 둘러싸는 중간세사에서 금입자를 관찰할 수 있었으며, 나머지 핵을 비롯한 다른 세포소기관이나 세포질에서는 관찰되지 많았다. 또한 기저돌기에서도 중간세사에서만 금입자를 관찰할 수 있었다. 이와 같은 본 실험의 결과는 박쥐 맨아래구역 뇌실막층에도 띠뇌실막세포가 존재하고 있으며, 이 세포의 발달된 긴 기저돌기는 띠뇌실막세포가 일반적인 뇌실막세포와는 다른 기능을 동면동물인 박쥐의 맨아래구역에서도 수행하고 있음을 암시하고 있으나 이를 확인하기 위해서 더욱 자세한 연구가 필요한 것으로 사료된다.

• 한국가축번식학회지
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• 제9권2호
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• pp.133-139
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• 1985

An immunoassay may be defined as an analytical procedure involving the competitive reaction between a limiting concentration of specific antibody and two populations of antigen, one of which is labelled or immobillized. The advent of immunoassay has revolutionised our knowledge of reproductive physiology and the practice of veterinary and clinical medicine. Radioimmunoassay (RIA) was the first of these methods to be developed, which meausred the analyte with good sensitivity, accuracy and precision (1,2). The essential components of RIA are:-(i) a limited concentration of antibodies, (ii) a reference preparation, and (iii) an antigen labelled with a radioisotope (usually tritium or iodine-125). Most procedures invelove isolating the antibody-bound fraction and measuring the amount of labelled antigen. Good facilities are available for scintilltion counting, data reduction nd statistical analysis. RIA is undergoing refinement through:-(i) the introduction of new techniques to separate the antibody-bound and free fractions which minimize the misclassification of labelled antigen into these compartments, and the amount of non-specfic binding. (3), (ii) the development of non-extration for the measurement of haptens (4), (iii) the determination of a, pp.rent free (i.e. non-protein bound) analytes (5), and (iv) the use of monoclonal antibodies(6). In 1968, Miles and Hales introduced in important new type of immunoassay which they termed immunora-diometric assay (IRMA) based on t도 use of isotopically labelled specific antibodies(7) in a move from limited to excess reagent systems. The concept of two-site IRMAs (with a capture antibody on a solid-phase, and a second labelled antibody to a different antigenic determinant of the analyte) has enabled the development of more sensitive and less-time consuming methods for the measurement of protein hormones ovar wide concentration of analyte (8). The increasing use of isotopic methos for diverse a, pp.ications has exposed several problems. For example, the radioactive half-life and radiolysis of the labelled reagent limits assay sensitivity and imposes a time limit on the usefulness of a kit. In addition, the potential health hazards associated with the use and disposal of radioactive cmpounds and the solvents and photofluors necessary for liquid scientillation counting are incompatable with the development of extra-laboratory tests. To date, the most practical alternative labels to radioisotopes, for the measurement of analytes in a concentration > 1 ng/ml, are erythrocytes, polystyrene particiles, gold sols, dyes and enzymes or cofactors with a visual or colorimetric end-point(9). Increased sensitivity to<1 pg/ml may be obtained with fluorescent and chemiluminescent labels, or enzymes with a fluorometric, chemiluminometric or bioluminometric end-point. The sensitivity of any immunoassay or immunometric assay depends on the affinity of the antibody-antigen reaction, the specific activity of the label, the precision with which the reagents are manipulated and the nonspecific background signal (10). The sensitivity of a limited reagent system for the measurement of haptens or proteins is mainly dependent upon the affinity of the antibodies and the smalleest amount of reagent that may be manipulated. Consequently, it is difficult in practice to improve on the sensitivity obtained with iodine-125 as the label. Conversely, with excess reagent systems for the measurement of proteins it is theoretically possible to increase assay sensitivity at least 1000 fold with alternative luminescent labels. To date, a 10-fold improvement has been achieved, and attempts are being made to reduce the influence of other variables on the specific signal from the immunoreaction.

• 한국조리학회지
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• 제23권1호
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• pp.66-78
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• 2017

"Sarcopenia", sarcopenia is an old age syndrome, and used to describe the reduction of skeletal muscle. Initially, it was thought that sarcopenia was only a senile disease characterized by degeneration of muscle tissue. However, its cause is widely regarded as multifactorial, with neurological decline, hormonal changes, inflammatory pathway activation, declines in activity, chronic illness, fatty infiltration, and poor nutrition, all shown to be contributing factors. Skeletal muscle mass can be measured by a variety of methods, currently, the commonly used methods are dual-energy X-ray scanning (DXA), computer tomography (CT), magnetic resonance imaging (MRI), etc. Muscular skeletal disorders can also be assessed by measuring appendicular skeletal muscle (ASM), particularly muscle tissue content. At the same time, sarcopenia refers to skeletal muscle cell denervation, mitochondrial dysfunction, inflammation, hormone synthesis and secretion changes and a series of consequences caused by the above process and is a progressive loss of skeletal muscle syndrome, which can lead to the decrease of muscle strength, physical and functional disorders, and increase the risk of death. Sarcopenia is mainly associated with the aging process, but also related to other causes such as severe malnutrition, neurodegenerative diseases, and disuse and endocrine diseases associated with muscular dystrophy, and it is the comprehensive results of multi-factors, so it is difficult to define that sarcopenia is caused by a specific disease. With the aging problem of the population, the incidence of this disease is increasingly common, and seriously affects the quality of the life of the elderly. This paper reviews the etiology and pathogenesis of myopathy, screening methods and diagnosis, the influence of eating habits, etc, and hopes to provide reference for the diagnosis and treatment of this disease. At present, adequate nutrition and targeted exercise remain the gold standard for the therapy of sarcopenia.

• Parasites, Hosts and Diseases
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• 제39권1호
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• pp.67-76
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• 2001

This experiment was focused on the characterization of anti- Toxoplasma monoclonal antibodies (mAbs) and the effect of mAbs on the parasite invasion of mouse peritoneal macrophages. Twenty eight mAbs including M110, M556, R7A6 and M62l were characterized by Ab titer, immunoglobulin isotyping and western blot pattern. Antibody titer (optical density) of 4 mAbs. Ml 10. M556. R7A6 and M62l. were 0.53,0.67, 0.45 and 0.39 (normal mouse serum; 0.19) with the same IgGl isotypes shown by Enzyme-linked immunosorbent assay (ELISA). Western blot analysis showed that Ml 10. M556. R7A6 and M62l reacted with the 33 kDa (p30),31 kDa (p28),43 kDa and 36 kDa protein. Immuno-gold labelling of mAbs M110, M556, R7A6 and M621 reacted with the surface membrane, dense granules and parasitophorous vacuolar membrane (PVM) , rhoptries and cytoplasm of tachyzoite, respectively. For in vitro assay, preincubation of tachyzoties with four mAbs, Ml 10, M556, R7A6 and M62l resulted in the decrease of the number of infected macrophages (p < 0.05) and the suppression of parasite multiplication at 18 h post-infection. Four monoclonal antibodies including Ml 10 (SAGI) were found to have an important role in the inhibition of macrophage invasion and T. gondii multiplication in vitro, and these mAbs may be suitable for vaccine candidates, diagnostic kit and for chemotherapy.

• 폴리머
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• 제29권4호
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• pp.331-337
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• 2005

일반적으로 꼬리부분에 카복실산을 갖는 알칸티올레이트는 바이오 물질의 고정화 활성물질로 사용되고 있다. 본 연구에서는 알칸티올레이트의 전기적 성질과 물리적 안정성을 향상시키기 위해 공액구조를 갖는 방향족계 티올레이트를 사용하여 cytochrome c와 같은 단백질을 고정화시켰다. 방향족계 자기조립 단분자막의 패턴 형성은 다음과 같은 공정으로 행하였다. 4'-Mercapto-biphenyl-4-carboxylic acid와 4-mercapto-[1,1';4',1']terphenyl-4'-carboxylic acid와 같은 방향족 티올레이트 분자를 금 기판에 흡착시킨 다음 네거티브 마스크를 가지고 원자외선 조사에 의해 산화반응을 시킨 후 deioniz water로 현상하였다. 공액계 자기조립 단분자막의 패턴형성과 전기적 성질은 STM과 AFM 측정을 통해 조사하였다. 또한 cytochrome c 또는 ferrocene amide를 패턴이 형성된 금 기판에 고정화시킨 다음 cyclic voltammeoy 측정을 통해 공액계 방향족 티올레이트의 전기적 활성을 검토하였다.

• 대한본초학회지
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• 제36권3호
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• pp.9-14
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• 2021

Objectives : The purpose of this study was to clarify the daily dose and uses of Goju-tang in Treatise on Cold Damage Diseases. Methods : We compared major editions of Treatise on Cold Damage Diseases with the original text of Supplement to the Essential Prescriptions Worth a Thousand Gold and chose the most appropriate text. If none of the existing texts seemed reasonable, We compared texts item by item. When there is nothing adequate even item by item, it was newly modified. These results were compared with currently distributed Pinellia Tuber and standards of eggs. Results : Goju-tang consisted of Goju(vinegar), Pinellia Tuber and an eggshell. Generally, a daily dose was extracted at once and taken 3 times a day, but in Goju-tang, 4 pieces of Pinellia Tuber and one-third Sheong of Goju(solvent) were used, which were one third of average volume. Pinellia Tuber was used cut in small pieces. And it was boiled gradually by low heat. Goju was mainly to reduce the pungency of Pinellia Tuber by cutting raphides of calcium oxalate. The Pot preparing medicines was eggshell which was chosen by its small size, because the volume of Pinellia Tuber and Goju was one-third of average volume. Since an egg shell is consisted of protein, it didn't react with vinegar(Goju). Conclusions : The 4 pieces of Pinellia Tuber and one-third Sheong of Goju(Vinegar) in the Goju-tang were one-third of average dose, and the eggshell was Pot preparing medicines.

• 한국작물학회지
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• 제56권1호
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• pp.8-13
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• 2011

국내 옥수수 유전자원을 계통육성하여 충남대학교 옥수수 유전 육종 실험실에서 새로 작성된 찰옥수수 교잡종을 2009년 5윌에 본 대학 옥수수 육종포장에서 재배하여 얻은 주요 식물학적 특성, 식미관련 물성검정, 영양가 분석 및 식미 평가에 대한 결과는 다음과 같다. 1. 공시된 찰옥수수 15개 교잡종에 대한 간장은 CNU08H-h39에서 235.7 cm, CNU08H-15에서 182.3 cm 범위로 공시종간에 큰 변이를 보였으며, 착수고 역시 Daehakchal Gold 1에서 83.7 cm, CNU08H-15에서 35.3 cm로 큰 차이를 보였다. 2. 이삭길이는 공시종 중에서 CNU08H-h121이 24.1 cm로 가장 길었고, CNU08H-71이 12.9 cm포 가장 작았으며 이삭직경은 Daehakehal Gold 1이 46.8 mm로 가장 굵고 CNU08H-69에서 26.1 mm로 가장 얇았다. 3. 공시종의 평균 당도는 15brix로 나타났는데 특히 CNU08H-15, CNU08H-35, CNU08H-h39, CNU08H-h102의 교잡종이 16 brix로 높은 편이었고 CNU08H-39에서 13 brix으로 가장 낮게 나타났다. 과피는 대조구인 연농찰이 $46.0{\mu}m$로 가장 두꺼웠고 CNU08H-39가 $23.0{\mu}m$로 가장 얇았다. 4. 생체이삭 내 단백질 함량은 대조구인 Ilmichal이 13.6%으로 가장 높게 나타났고, CNU08H-39가 8.6%으로 가장 낮았다. 아밀로펙틴 함량은 CNU08H-15의 교잡종이 94.5%로 가장 높았고 CaNU08H-71 에서 90.5%로 가장 낮았다. 5. 공시종에 대한 관능 평가 결과 대학찰골드 1호, CNU08H-h121 그리고 CNU08H-39순으로 높게 나타났으며 대조구인 연농찰 역시 비교적 높게 나타났다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권6호
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• pp.353-359
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• 2013

Purpose: Extensive research is actively ongoing for development of an ideal bone substitute that meets the gold standard. Tooth was selected as a donor site for evaluation of potentials in bone substitutes based on its similar chemical compositions to alveolar bone. Previous studies have evaluated inorganic components of autogenous tooth bone graft material (AutoBT) and osteoconductivity. In continuation from the previous studies, the current study was conducted for analysis of organic components and evaluation of osteoinductivity of AutoBT. Methods: Forty-six extracted teeth were collected from actual patients (Korea Tooth Bank, R&D Institute). Extracted teeth were processed into AutoBT and implanted in dorsal subcutaneous muscular tissues of 15 athymic mice. Biopsy samples were harvested at two, five, and eight weeks. The Bradford assay, sodium dodecyl sulphate polyacrylamide gradient gel, and western blotting were performed for investigation of organic contents of AutoBT. Results: Histology analyses showed signs of new bone formation as early as two weeks. Results of the Bradford assay indicated the existence of noncollagenous proteins (NCP). 0.29% (2.89 mg/g) of proteins were extracted by weight in the root portion of AutoBT; 0.02% (0.029 mg/g) and 1.79% (17.93 mg/g) of proteins were measured by weight in crown and block-form of AutoBT, respectively. However, recombinant human bone morphogenetic protein-2 was not observed in AutoBT. Conclusion: Within the limitation of the current study, AutoBT induced new bone formation by NCP embedded in dentin.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제18권3호
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• pp.411-427
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• 1996

The purpose of this study was to observe the healing process and the distribution of fibronectin in injured condylar cartilage and bone by using LM and SEM. In order to perform this study, 40 male rat, weighing about 250g were selected. Under general anesthesia with Pentobarbital sodium, condylar cartilage and neck bone were resected. Then, the wound was irrigated with saline and closed with 5-0 chromic catgut and 4-0 silk by layer-to-layer suturing. The experimental rats were sacrificed by perfusion with 3% paraformaldehyde at 1st and 4th week after operation. The condylar process and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. The histological observation of the specimens in LM level was performed after H-E stain and Azan stain. For localization of fibronectin, immunostaining was achieved by the avidin-biotin complex method. To study the change on condylar surface, the specimens were dehydrated, dried, gold coated and were observed with a scanning electron microscope(Hitachi S-2300). The results were as follows ; 1. The cartilage group and the bone group were repaired with epiphyseal cartilage layer on the cut surface as the normal control group. 2. The cut surface was repaired more quickly in the cartilage group than in the bone group. 3. Chondrocytes, diferentiated during healing, were stained strongly to anti-fibronectin, and fibronectin was supposed to participatein chondrocyte differentiation and cartilagenous matrix formation. 4. Fibronectin was distributed more in the new bone than in the old bone, and the osteoblasts surrounding it were also stained strongly. Fibronectin was supposed to participate in new bone matrix formation. 5. Fibronectin is supposed to be associated with the differentiation, migration and adhesion of chondrocyte and osteoblast and to participate in endochondral bone formation.