• Title/Summary/Keyword: Propagation media

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Propagation of a Rare Variety Daphne kiusiana Miq. In Korea Through Softwood Cuttings (한반도 희귀식물인 백서향의 녹지삽목을 통한 증식)

  • Ro, Na-Young;Song, Eun-Young;Kim, Seong-Cheol;Ko, Ho-Chul;Lee, Sok-Young
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.246-250
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    • 2010
  • This study was conducted to find out the propagation method of a rare variety Daphne kiusiana Miq. in Korea through softwood cuttings. Daphne kiusiana Miq are the evergreen broad leaf tree which have lived in the southern part of Korea. The flowering time of Daphne kiusiana Miq is early spring and the flower has an attractive fragrance. Generally, plants are dioecious and only the male plants are found in Korea. Propagation by seed is very difficult in this tree. Softwood cuttings were taken to establish an efficient propagation method in Daphne kiusiana Miq Cuttings were grown in perlite:vermiculite (1 : 1), perlite, vermiculite, sand rooting media under shaded greenhouses during summer in 2007. The rooting rates of cuttings were 86.7%, 95%, 75% and 95% in perlite:vermiculite (1 : 1), perlite, vermiculite and sand media, respectively. Softwood cuttings taken on July and rooting at $27^{\circ}C$ were significantly improved the growth and rooting rates. Softwood cutting can be an effective means of propagation in Daphne kiusiana Miq.

Modeling of Elastodynamic Problems in Finite Solid Media (유한 고체내 탄성동역학 문제의 모델링)

  • Cho, Youn-Ho
    • Journal of the Korean Society for Nondestructive Testing
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    • v.20 no.2
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    • pp.138-149
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    • 2000
  • Various modeling techniques for ultrasonic wave propagation and scattering problems in finite solid media are presented. Elastodynamic boundary value problems in inhomogeneous multi-layered plate-like structures are set up for modal analysis of guided wave propagation and numerically solved to obtain dispersion curves which show propagation characteristics of guided waves. As a powerful modeling tool to overcome such numerical difficulties in wave scattering problems as the geometrical complexity and mode conversion, the Boundary Element Method(BEM) is introduced and is combined with the normal mode expansion technique to develop the hybrid BEM, an efficient technique for modeling multi mode conversion of guided wave scattering problems. Time dependent wave forms are obtained through the inverse Fourier transformation of the numerical solutions in the frequency domain. 3D BEM program development is underway to model more practical ultrasonic wave signals. Some encouraging numerical results have recently been obtained in comparison with the analytical solutions for wave propagation in a bar subjected to time harmonic longitudinal excitation. It is expected that the presented modeling techniques for elastic wave propagation and scattering can be applied to establish quantitative nondestructive evaluation techniques in various ways.

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Several Factors Affecting In Vitro Propagation of Climacium japonicum (나무이끼(Climacium japonicum)의 기내배양에 영향을 미치는 몇 가지 요인)

  • Ahmed, Md. Giush Uddin;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.1
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    • pp.15-22
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    • 2010
  • These investigations were conducted to standardize several chemical and physical environments affecting in vitro propagation of gametophytes of Climacium japonicum. Propagation of this moss species was established on basal medium containing Knop macro salts and Nitsch and Nitsch trace elements. Primary cultures were initiated from apical shoots of gametophytes. Gametophyte production was accessed using chopped gametophytes, apical shoots and basal shoots. Seven ty pes of culture media and four concentrations of total nitrogen and five strengths of sucrose were tested for in vitro gametophyte production. Light and temperature factors were also evaluated. Apical shoots were the greatest among three types explants used for gametophyte propagation. Medium containing Knop macro salts and Nitsch and Nitsch trace elements was more effective than other types of media. Higher sucrose concentrations showed a positive effect on the elongation and multiplication of gametophytes. Both nitrogen deficiency and excessiveness inhibited gametophyte growth. Light intensity variation showed highly significant changes in numbers, length and fresh weight of gametophytes. Optimum light intensity for gametophyte growth seemed to be around 3000-4000 lx. Both lower and higher temperature had a negative effect on gametophyte propagation and production. This study will provide large scale and high quality propagules, and effective moss propagation system.

In Vitro Shoot Tip Culture of Haemaria discolor (Haemaria discolor 경정의 기내배양)

  • 왕영조;정재동;최수옥;지선옥
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.4
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    • pp.227-231
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    • 1994
  • The experiments were conducted to obtain culture conditions on optimal cultural conditions for propagation of a topical orchid, Haemaria discolor, through shoot tip cultrue in vitro. Survival percentage of shoot tip explant, in initial culture media was higher in H$_3$P$_4$ medium than in Murashige and Skoog's medium. Explants, cultured in H$_3$P$_4$ medium containing 1.0 mg/L kinetin, showed more shoot elongation when compared with those on other media. When the distal part of stem (pseudobulb) was longitudinally sectioned into half and cultured in H3P4 medium containing 0.1 mg/L 2ip, 0.1, or 1.0 mg/L kinetin, shoots from axillary bud were much more elongated under light condition.

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In vitro propagation of Phaleonopsis hybrid 'Little gem' by culturing apical part and axillary bud of flower stalk

  • Chung, Mi Young;Naing, Aung Htay;Khatun, Khadiza;Ahn, Hyung Geun;Lim, Ki Byung;Kim, Chang Kil
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.438-443
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    • 2016
  • The in vitro propagation of the commercially important Phalaeonopsis hybrid 'Little gem' was achieved by culturing the apical part and axillary buds excised from flower stalks. The explants were cultured on 5 different basal media: $3.0{\cdot}L^{-1}$ Hyponex and $4.0{\cdot}L^{-1}$ peptone ($H_3P_4$) and Murashige & Skoog (MS) media were shown to be suitable for shoot regeneration. The MS medium supplemented with $5.0mg{\cdot}L^{-1}$ 6-benzylaminopurine (BA) was found to be more efficient for shoot regeneration. However, the number of shoots induced by axillary buds was higher than that induced by the apical part. Incubation of the apical part under darkness for one week, as well as of the explants in the same medium with activated charcoal (AC) $0.5g{\cdot}L^{-1}$ promoted shoot regeneration and shoot growth; similar growth was not observed with axillary buds.

Feasibility for Ultrasound Pad Material for the Evaluation Axillary Region of Automated Breast Ultrasound Equipment (자동유방초음파 장비의 액와부 평가를 위한 초음파 패드 물질의 타당성)

  • Seo, Eun-Hee;Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.41 no.3
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    • pp.231-240
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    • 2018
  • Automated breast ultrasound (ABUS) equipment is a new innovative technique for 3D automatic breast scanning, but limited for the examination in the concave axillary region. The purpose of this study was to determine feasible candidate materials for the ultrasonic wave propagation media in ABUS, enabling the evaluation of the axillary region. Ultrasonography was performed using an ABUS system ($Invenia^{TM}ABUS$, GE, USA) on the ultrasound-specific phantom (UC-551M-0.5, ATS Laboratories, USA) covered by different candidate materials. The validity of feasible candidate materials was evaluated by image quality. Three independent radiological technologists, with more than 10 years of experience, visually assessed on the images. The inter-observer agreements according to the candidate materials were tested using Cronbach's alpha. Unenveloped solidified carrageenan can be a feasible material for the use of ABUS with excellent test reliability. Therefore, the coverage of the axillary region with carrageenan may be effective for ABUS which was originally developed for the convex anatomic structure as female breast.

Source & crustal propagation effects on T-wave envelopes

  • Yun, Suk-Young;Park, Min-Kyu;Lee, Won-Sang
    • 한국지구물리탐사학회:학술대회논문집
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    • 2010.10a
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    • pp.27-27
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    • 2010
  • There have been several studies about empirical relation between seismic source parameters(e.g., focal mechanisms, depths, magnitudes, etc.) and T-wave observation. In order to delineate the relation, numerical and theoretical approaches to figure out T-wave excitation mechanism are required. In an attempt to investigate source radiation and wave scattering effects in the oceanic crust on T-wave envelopes, we perform three-dimensional numerical modeling to synthesize T-wave envelopes. We first calculate seismic P- and SV-wave energy on the seafloor using the Direct Simulation Monte Carlo based on the Radiative Transfer Theory, which enables us to take into account both realistic seismic source parameters and wave scattering in heterogeneous media, and then estimate excited T-wave energy by normal mode computation. The numerical simulation has been carried out considering the following different conditions: source types (strike and normal faults), source depths (shallow and deep), and wave propagation through homogeneous and heterogeneous Earth media. From the results of numerical modeling, we confirmed that T-wave envelopes vary according to spatial seismic energy distributions on the seafloor for the various input parameters. Furthermore, the synthesized T-wave envelopes show directional patterns due to anisotropic source radiation, and the slope change of T-wave envelopes caused by focal depth. Seismic wave scattering in the oceanic crust is likely to control the shape of envelopes.

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