• Title/Summary/Keyword: Promoting

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Isolation and Characterization of Plant Growth Promoting Rhizobacterium Bacillus subtilis YK-5 from Soil (토양에서 식물생육촉진 활성을 가진 균주 Bacillus subtilis YK-5의 분리 및 특성)

  • Yeo, Soo-Hwan;Yook, Young-Min;Kim, Hyun-Soo
    • KSBB Journal
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    • v.24 no.4
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    • pp.334-340
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    • 2009
  • For the isolation of a plant growth-promoting rhizobacteria, strain YK-5 was selected from approximately 400 thermostable strains isolated from special soil samples. Strain YK-5 produced an antifungal compound, and optimum carbon and nitrogen sources for the production of the antifungal compound were investigated against Aspergillus flavus as a test strain. Modified LB medium containing 1% peptone, 1% yeast extract and 5% black sugar was determined to be the optimal medium for growth and antifungal compound production. Culture broth of strain YK-5 potently inhibited growth of the phytopathogenic fungus Fusarium oxysporum KACC 40052 for 7 days. The plant growth-promotion function of strain YK-5 was tested against radish and rice in pot trials. Leaf number, plant height and root length in YK-5-treated radish markedly exceeded (> 60%) those of untreated radish. Leaf length and white rootlet development were markedly more prominent than in commercially-treated rice plants. Strain YK-5 was determined to be Bacillus subtilis YK-5 by physiological, chemotaxonomical, and phylogenetical analyses.

A Comparative Study on the Characteristics of Accelerated aging at Low and High Temperatures of the Fluorocarbon Rubber Composites (불소 고무복합체의 저온과 고온촉진노화 특성에 대한 비교 연구)

  • Park, JeongBae;Lee, BeomCheol;Jeong, YoonSeok;Park, SungHan
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2017.05a
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    • pp.915-922
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    • 2017
  • The study on the thermal and oil resistance rubber composite, 2016. [6] predicted the lifetime of Fluorocarbon Rubber by accelerating aging at high temperature ($150^{\circ}C$, $175^{\circ}C$, $200^{\circ}C$). general rubber products are likely to exhibit different properties depending on the degradation factors such as temperature, humidity, ozone, light, emulsion, mechanical and electrical stress. To solve these problems, We compared the rate of change about tensile strength, elongation rate, volume change rate, weight change rate, thickness change rate, thermal conductivity in low temperature promoting aging on the basis of predictive lifetime of high temperature promoting aging. As a result of the review, the required life expectancy was satisfied, but there was a slight difference in the rate of change between the high-temperature promoted aging life result and the low temperature promoted aging life result. The cause was a reduction in "tensile strength / elongation" and an increase in "volume / weight / thickness" caused by the main chain decomposition of fluorine rubber due to aging at high temperature promoting aging. However, the low temperature promoting aging was caused by the curing reaction of fluorine rubber at $80^{\circ}C$. The tensile strength / elongation and volume / weight / thickness changes were small.

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A Study on the Health Status and Health Promoting Behavior of Older Adults in a Rural Area (일 농촌노인의 건강상태 및 건강증진행위에 관한 연구)

  • Kim, Jung-Soon
    • Research in Community and Public Health Nursing
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    • v.12 no.1
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    • pp.187-201
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    • 2001
  • This study was conducted to investigate the health status and health promoting behavior of older adults in rural area. The design of research was descriptive study. 883 older adults over sixty years living in the rural area of Pusan city were surveyed from July 9. 1999 to July 20. 1999 through direct interview using a questionnaire and physical examination. The collected data were analyzed for percentage. mean. Chi square-test. ANOVA using the SPSS computerized program. The main results were as follows: 1. 43.4% of subjects lived alone or with only partner. 2. 37.13% of subjects had perceived own health condition as bad. 3. Major 'chronic diseases that the subjects were suffered were diabetes(25.94%) and hypertension(9.11%). 4. The mean score of perceived depression was 17.71 of 44. 5. 87.98% of subjects replied that they had good relationship with their family and friends. 6. 8.57% of subjects were identified as over weight. while 10.85% were low weight. 7. 29.93% of subjects replied that they were smoking. By the group. the rate of smoking of man was significantly higher than women. and lower age group than higher age group, and higher education group than lower education group. 8. 70.38% of subjects didn't practice exercise. By the group, the rate of exercise of woman was significantly lower than man, and higher age group than lower age group, and lower education group than higher education group. 9. 12.33% of subjects replied for the frequency of drinking as more three -times a week. By the groups. man showed significantly more frequency than woman. the lower age group than higher age group, the lower education group than the higher education group. 11. The mean score of nutrition state was 3.73 which means moderate risk state. 12. The 57.53% of subjects replied, their sleeping time as below 7 hours. 13. The 15.75% of subjects had experience a periodical inspection. In conclusion, older adults in rural area were identified having various health risk factor, Looking at the results. It is necessary to develope health promotion program which enhances older adults to practice health promoting behavior and to manage their chronic disease.

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Plant Growth-promoting Ability by the Newly Isolated Bacterium Bacillus aerius MH1RS1 from Indigenous Plant in Sand Dune (해안 사구에서 서식하는 토착식물로부터 분리된 근권미생물 Bacillus aerius MH1RS1의 식물성장 촉진 능력 연구)

  • Lee, Eun Young;Hong, Sun Hwa
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.10
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    • pp.687-693
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    • 2013
  • Coastal sand dunes have been seriously damaged caused by the development thoughtless for the environment and coastal erosion and destruction due to artificial structures like coast roads and breakwater. Hereupon, in this study we made a library of rhizobacteria that have the plant growth-promoting ability for plant rhizosphere of indigenous plants inhabiting in a coastal sand dune as well as the strong tolerance to salt, and evaluated the plant growth-promoting ability of these strains. Furthermore, we evaluated the effect of rhizobacteria on the growth rate of saline tolerant plants in sandy soil; selected out the most useful micro-organism for the restoration of a damaged sand dune. The effect of inoculation of strains selected from the first experiment on the growth of Peucedanum japonicum and Arundo donaxes planted in a coastal sand dune was evaluated. As a result, Bacillus aerius MH1RS1 had plant growth promoting activities: indole acetic acid (IAA) production, siderophores and 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity, and also had a salinity tolerance. Also, in case of Peucedanum japonicum, the length of stems and weights of roots were enhanced by the inoculation of B. aerius MH1RS1. Fresh weights of stems and weights of roots in experimental group were, in particular, increased by 25% comparing with the control group. For an Arundo donax in experimental group, plant length increased by 18%, and weight of roots by 20% which is significant.

Isolation of Agrobacterium sp. BE516 from the Root of Miscanthus sacchariflorus and Its Plant Growth Promoting Activity (물억새 뿌리로부터 Agrobacterium sp. BE516 균주의 분리 및 식물생육촉진활성)

  • Kang, Hye-Young;Park, Dong-Jin;Lee, Jae-Chan;Kwon, Mi-Kyung;Kim, Seung-Bum;Kim, Chang-Jin
    • Journal of Applied Biological Chemistry
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    • v.55 no.2
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    • pp.129-133
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    • 2012
  • To exploit plant growth promoting bacteria in the roots of Miscanthus sacchariflorus, a biomass energy crop, total 64 bacteria were isolated. For the investigation of plant growth promoting effects from the isolated bacteria, production of indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were tested and other cultural conditions were examined. As results, 8 isolates showed plant growth promoting effects on the M. sacchariflorus and an isolate designated Agrobacterium sp. BE516 has the highest activity by enhancing the shoot elongation over 2-fold than the control. Agrobacterium sp. BE516 produced 64 ${\mu}g$ IAA per mL and showed ACC deaminase activity which is involved in the resistance to environmental stress such as high salt and drought. It could grow at low temperature in the range from 4 to $15^{\circ}C$, at pH 4.0 and at 4% NaCl. These results indicate that the Agrobacterium sp. BE516 can be useful as a bio-fertilizer for M. sacchariflorus under the stressed conditions.

Plant Growth-Promoting Activity Characteristics of Bacillus Strains in the Rhizosphere (근권에 존재하는 Bacillus 속 균주들의 식물 생장 촉진 활성 특성)

  • Oh, Ka-Yoon;Kim, Ji-Youn;Lee, Song Min;Kim, Hee Sook;Lee, Kwang Hui;Lee, Sang-Hyeon;Jang, Jeong Su
    • Microbiology and Biotechnology Letters
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    • v.49 no.3
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    • pp.403-412
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    • 2021
  • This study aimed to identify plant growth-promoting activity, phytopathogenic fungi growth inhibitory activity, mineral solubilization ability, and extracellular enzyme activity of the genus Bacillus in soil and the rhizosphere. With regards to antifungal activity against phytopathogenic fungi, DDP257 showed antifungal activity against all 10 pathogenic fungi tested. ANG20 showed the highest ability to produce indole-3-acetic acid, a plant growth-promoting factor (70.97 ㎍/ml). In addition, 10 species were identified to have 1-aminocyclopropane-1-carboxylate deaminase production ability, and most isolates showed nitrogen fixation and siderophore production abilities. Thereafter, the isolated strains' ability to solubilize minerals such as phosphate, calcite, and zinc was identified. With extracellular enzyme activity, the activity appeared in most enzymes. In particular, all the strains showed similar abilities for alkaline phosphatase, esterase (C4), acid phosphatase, and naphtol-AS-BI-phosphohydrolase production. This result was observed because the genus Bacillus secreted various organic substances, antibiotics, and extracellular enzymes. Therefore, through the results of this study, we suggest the possibility of using strains contributing to the improvement of the soil environment as microbial agents.

Growth promotion and root development of Nicotiana tabacum L. by plant growth promoting fungi (PGPF) (식물 생장 촉진 진균에 의한 담배의 생장 촉진과 뿌리 발달)

  • Hong, Eunhye;Lee, Jinok;Kim, Sujung;Nie, Hualin;Kim, Young-Nam;Kim, Jiseong;Kim, Sunhyung
    • Journal of Plant Biotechnology
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    • v.47 no.4
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    • pp.337-344
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    • 2020
  • Plant growth-promoting microorganisms promote plant growth by supplying nutrients to roots and interacting with the intrinsic factors in plants through volatile organic compounds (VOCs). In this study, we evaluated the effect of UOS, plant growth-promoting fungi (PGPF) isolated from previous study, on the growth of Nicotiana tabacum L. var Xanthi nc. Phylogenetic analysis and GC-MS were used to identify the fungal species and the VOCs emitted by the UOS, respectively. The fresh weight of UOS-treated Nicotiana tabacum L. was 3.8 and 4.2-fold higher than that of the control groups grown in vertical and I-plates, respectively. Moreover, in the UOS-treated plants, the length of the primary root was half and the number of lateral roots were twice compared to those in control plants. The UOS was identified as Phoma sp. by studying spore and mycelial morphology and using phylogenetic analysis. GC-MS revealed that the VOC emitted by the UOS was hexamethylcyclotrisiloxane (D3). These results suggest that the UOS of Phoma sp. influences plant growth and root development through D3. We expect this UOS and its VOC, D3 to be utilized in the future to increase growth and enhance yield for other plants.

Influence on Adjustment of University Life among Nnursing Students (간호대학생의 대학생활 적응에 미치는 영향)

  • Cha, Hyun Su
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.8
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    • pp.381-391
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    • 2019
  • The purpose of this study was to investigate the factors having an influence on adjusting to the university life of freshmen and sophomore nursing students. The data was collected from questionnaires that were filled out by 150 nursing students in one school from November 1, 2018 to November 20, 2018. The data was analyzed by the SPSS 22.0 program (frequency, ANOVA, Pearson's correlation and multiple regression analysis). The mean score of university life adjustment was $75.85{\pm}9.43$ (range: 25~125). A negative correlation was found between adjustment to university life and stress, A positive correlation was found between adjusting to university life and self efficacy. Negative correlation was found between stress and self efficacy. The factors influencing the adjustment of freshmen and sophomore nursing students to university life were health promoting behavior (sleeping time, snacks, drinking, and leisure time or having a hobby), stress and self efficacy. In addition, health promoting behaviors, stress and self efficacy accounted for 13.9% of the variance in adjusting to university life. The results of this study suggest that health promoting behavior, stress and self efficacy are important to the adjustment to university life for freshmen and sophomore nursing students. Further research on improving health promoting behavior, reducing stress and improving self efficacy is warranted. An intervention program that includes these significant variables of such subjects should be developed to improve adjusting to the university life of nursing students.

Current Perspectives on the Effects of Plant Growth-promoting Rhizobacteria (식물생장촉진 근권미생물의 영향에 대한 연구 현황 및 전망)

  • Le, Thien Tu Huynh;Jun, Sang Eun;Kim, Gyung-Tae
    • Journal of Life Science
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    • v.29 no.11
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    • pp.1281-1293
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    • 2019
  • The rhizosphere is the active zone where plant roots communicate with the soil microbiome, each responding to the other's signals. The soil microbiome within the rhizosphere that is beneficial to plant growth and productivity is known as plant growth-promoting rhizobacteria (PGPR). PGPR take part in many pivotal plant processes, including plant growth, development, immunity, and productivity, by influencing acquisition and utilization of nutrient molecules, regulation of phytohormone biosynthesis, signaling, and response, and resistance to biotic- and abiotic-stresses. PGPR also produce secondary compounds and volatile organic compounds (VOCs) that elicit plant growth. Moreover, plant roots exude attractants that cause PGPR to aggregate in the rhizosphere zone for colonization, improving soil properties and protecting plants against pathogenic factors. The interactions between PGPR and plant roots in rhizosphere are essential and interdependent. Many studies have reported that PGPR function in multiple ways under the same or diverse conditions, directly and indirectly. This review focuses on the roles and strategies of PGPR in enhancing nutrient acquisition by nutrient fixation/solubilization/mineralization, inducing plant growth regulators/phytohormones, and promoting growth and development of root and shoot by affecting cell division, elongation, and differentiation. We also summarize the current knowledge of the effects of PGPR and the soil microbiota on plants.

A Musical Play Program for Promoting Interaction Between Immigrant and Korean Preschoolers (다문화통합어린이집 내 이주배경아동-한국가정아동 간 또래상호작용 증진을 위한 음악극 프로그램 개발)

  • Kim, ChanMi
    • Journal of Music and Human Behavior
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    • v.19 no.1
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    • pp.21-41
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    • 2022
  • The purpose of this research was to develop and verify applicability of a musical play program aimed at promoting interaction between immigrant preschoolers and Korean preschoolers. A daycare center serving the preschool-age children of Korean nationals and immigrant families were the site for this research. Utilizing the elements of a musical play (e.g., role playing, narration, and joint music activity), the program was developed for improving interaction among preschoolers, while promoting intercultural acceptance by providing the opportunity to be exposed to music from different cultures and engage in the joint activity using such music. For the purpose of investigate the applicability of the developed program, the program was implemented with two groups of preschoolers and each group received the musical play program for 30 minutes twice a week for a total of 6 sessions. The results of this preliminary study indicate that the musical play program promoted interaction between peers from different cultural background. Based on the results of this study, musical play programs may be helpful in promoting interactions between preschoolers from different cultural backgrounds and increase of acceptance of immigrant students in Korean daycares and schools.