Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of Agrobacterium sp. BE516 from the Root of Miscanthus sacchariflorus and Its Plant Growth Promoting Activity

물억새 뿌리로부터 Agrobacterium sp. BE516 균주의 분리 및 식물생육촉진활성

  • Kang, Hye-Young (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Dong-Jin (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Mi-Kyung (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Seung-Bum (Department of Microbiology & Molecular biology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Chang-Jin (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2012.03.26
  • Accepted : 2012.05.25
  • Published : 2012.06.30
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Abstract

To exploit plant growth promoting bacteria in the roots of Miscanthus sacchariflorus, a biomass energy crop, total 64 bacteria were isolated. For the investigation of plant growth promoting effects from the isolated bacteria, production of indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were tested and other cultural conditions were examined. As results, 8 isolates showed plant growth promoting effects on the M. sacchariflorus and an isolate designated Agrobacterium sp. BE516 has the highest activity by enhancing the shoot elongation over 2-fold than the control. Agrobacterium sp. BE516 produced 64 ${\mu}g$ IAA per mL and showed ACC deaminase activity which is involved in the resistance to environmental stress such as high salt and drought. It could grow at low temperature in the range from 4 to $15^{\circ}C$, at pH 4.0 and at 4% NaCl. These results indicate that the Agrobacterium sp. BE516 can be useful as a bio-fertilizer for M. sacchariflorus under the stressed conditions.

비식량 바이오매스 대상작물인 억새의 생육을 촉진하는 균주를 선발하기 위하여 충북 청원군 대청호 주변에서 서식하는 물억새 뿌리로부터 64균주를 분리하였다. 분리균의 식물생육촉진 활성을 확인하기 위하여 옥신, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase 생선능 및 기타 배양조건에 따른 생육 특성을 조사한 결과, 8균주가 선발되었으며 Agrobacterium sp. BE516 균주가 억새 줄기의 생육을 2배 이상 증가시켜 가장 활성이 뛰어난 균주로 선발되었다. Agrobacterium sp. BE516 균주는 식물호르몬 indole acetic acid를 64 ${\mu}g/mL$ 생산하고, 염 및 건조 등의 환경 스트레스 저항성 ACC deaminase 를 생산하며, $4-15^{\circ}C$, pH 4.0 및 4% NaCl 농도에서 생육하였다. 따라서, Agrobacterium sp. BE516 균주가 간척지와 같은 조건불리지역에서 비식용 바이오매스 대상작물인 억새의 생육을 촉진시키기 위한 미생물비료제 개발에 유용한 균주임을 확인하였다.

Keywords

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