• The Journal of Korean Obstetrics and Gynecology
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• v.32 no.1
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• pp.1-14
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• 2019

Objectives: This study was designed to investigate the effect of Bombycis corpus on reproductive dysfunction caused by aging. Methods: The experimental group was divided into three groups: a control group consisting of 8-week-old male ICR mice without any treatment, An aging-elicited group (AE group) consisting of 50-week-old ICR male mice without any treatment, and a Bombycis corpus treatment group (BC group) consisting of 50-week-old ICR male mice with treatment Bombycis corpus extract (0.78 g/kg/day) for 6 months. After 6 months, histochemistry and immunohistochemistry of the testis were performed to investigate the effects of Bombycis corpus on the reproductive dysfunction caused by aging. Results: In the first step, Bombycis corpus increased spermatogenesis and distribution of sertoli cells in the seminiferous tubule, increased BrdU positive reaction in the spermatogonium at the basal part of the seminiferous tubule, and decreased the apoptosis of Sertoli cells in the seminiferous tubule. In addition, Bombycis corpus increased AR positive in Sertoli cells and $17{\beta}-HSD$ positive in leydig cells. Finally, Bombycis corpus decreased 8-OHdG positivite reaction in the spermatids of the seminiferous lumen, caspase-3 positivity in leydig cells, and HDAC1 positivite reaction in sertoli cells. Conclusions: These results suggest that Bombycis corpus increases spermatogenesis, decreases apoptosis of leydig cells and Sertoli cells, increases the production and action of testosterone in the testis, and inhibits DNA damages and DNA transcripts decrease in the testis, Thereby improving reproductive dysfunction caused by aging.

• Membrane Journal
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• v.29 no.2
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• pp.105-110
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• 2019

Porous membranes are widely used in industry for removing particulate matter. Unlike conventional porous membrane fabrication methods, the solution spreading phase separation method can form pores very simply. The first step is to wet the mesh with the support layer, then to let the polysulfone solution flow into a solvent without water. The solvent is readily vaporized and the polysulfone is made into a thin film. When the polysulfone solution is mixed with water to form pores, the pore size can be adjusted according to the concentration ratio of the polysulfone solution. The thickness of the membrane is easily controlled by the concentration of the solution. The porous separator has the formation of meshes intact and is very useful for forming a three-dimensional structure. The solution spreading phase separation method proposed in this study is characterized by its high cost competitiveness compared with conventional membranes due to its low production cost and easy process control.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.960-966
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• 2018

Communication antennas used at sea have been developed remarkably. However, the speed of this development is insufficient when compared with user demands. Therefore, we developed an antenna for UWB/Bluetooth that operates on 3 [GHz] and 5.72 [GHz] bands in order to use the high-speed communication network system which improved antenna miniaturization, gain and radiation patterns. To improve bandwidth, a microstrip patch antenna was selected and CST Microwave Studio 2014 program was used. Through the program, we calculated the slot width, length, transmission line width, etc. using a theoretical formula at each step. These figures were checked through simulation to see if they meet production standards. UWB for high-speed wireless communication for short-range communication at sea, Bluetooth for exchanging information at a short distance by connecting each device, and corresponding technology can be easily utilized.

• Composites Research
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• v.32 no.3
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• pp.134-140
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• 2019

Graphene nanoplatelets (GNP), one of the graphene derivatives is famous as the most proper candidate for industrial applications. However, current performance of GNPs as reinforcing filler in composites is limited by their agglomeration and physicochemical heterogeneity. Herein, an approach to produce non-covalently functionalized GNPs (F-GNPs) is reported which possesses potential to be extended as the industrial level of mass production. The one-step functionalization process uses melamine, a low-cost chemical, to prevent agglomeration and dispersion in polar solvents. Furthermore, a purification strategy called salting-out process based on differences in the dispersibility of the individual F-GNP flakes is reported to separate F-GNPs. The functionalization and separation process developed in this paper provides a strategy to use GNPs at the industrial level in composite applications.

• The Korean Journal of Archival Studies
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• no.34
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• pp.107-163
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• 2012

The share of the internet has grown significantly in terms of usage and level of influence. Among the internet media, OhmyNews is considered a alternative media representing Korea's independent internet news, and a typical example displaying characteristics of the internet news. The processing step can be divided as five stage, with recorded information and applicable technical element extractable from each stage. In this thesis paper, we have analyzed domestic and overseas metadata standard examples to devise metadata design plans. Items to be focused when redesigning metadata based on domestic and foreign case studies are as follows: First, user access should be convenient; second, connection with related information should be considered; third, accumulation of production, management, usage, storage, and action history should be considered; and fourth, the design should allow higher utilization of contents. In depth researches over internet media are already being carried out in external academic disciplines including the media information studies, information and communication studies, and communications studies. We expect that it will also be necessary to consider such researches over the internet media for mass media record management research going forward.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.4
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• pp.508-518
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• 2019

Objective: This research aimed to determine biological pathways and protein-protein interaction (PPI) networks for 305-d milk yield (MY), 305-d fat yield (FY), and age at first calving (AFC) in the Thai multibreed dairy population. Methods: Genotypic information contained 75,776 imputed and actual single nucleotide polymorphisms (SNP) from 2,661 animals. Single-step genomic best linear unbiased predictions were utilized to estimate SNP genetic variances for MY, FY, and AFC. Fixed effects included herd-year-season, breed regression and heterosis regression effects. Random effects were animal additive genetic and residual. Individual SNP explaining at least 0.001% of the genetic variance for each trait were used to identify nearby genes in the National Center for Biotechnology Information database. Pathway enrichment analysis was performed. The PPI of genes were identified and visualized of the PPI network. Results: Identified genes were involved in 16 enriched pathways related to MY, FY, and AFC. Most genes had two or more connections with other genes in the PPI network. Genes associated with MY, FY, and AFC based on the biological pathways and PPI were primarily involved in cellular processes. The percent of the genetic variance explained by genes in enriched pathways (303) was 2.63% for MY, 2.59% for FY, and 2.49% for AFC. Genes in the PPI network (265) explained 2.28% of the genetic variance for MY, 2.26% for FY, and 2.12% for AFC. Conclusion: These sets of SNP associated with genes in the set enriched pathways and the PPI network could be used as genomic selection targets in the Thai multibreed dairy population. This study should be continued both in this and other populations subject to a variety of environmental conditions because predicted SNP values will likely differ across populations subject to different environmental conditions and changes over time.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.1
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• pp.50-61
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• 2019

The concern on the greenhouse gas emissions is increasing globally. Especially, the greenhouse gas emission from fisheries is an important issue from the Paris Climate Change Accord in 2015. Furthermore, the Korean government has a plan to reduce the GHG emissions as 4.8% compared to the BAU in fisheries until 2020. However, the investigation on the GHG emissions from Korean fisheries rarely carried out consistently. Therefore, the quantitative analysis of GHG emissions from Korean fishery industry is necessary as a first step to find a relevant way to reduce GHG emissions from fisheries. The purpose of this research is to investigate which degree of GHG emitted from the major offshore fisheries such as offshore gillnet fishery, offshore longline fishery, offshore jigging fishery and anchovy drag net fishery. Here, we calculated the GHG emissions from the fisheries using the Life Cycle Assessment method. The system boundary and input parameters for each process level are defined for the LCA analysis. The fuel use coefficients of the fisheries are also calculated according to the fuel type. The GHG emissions from sea activities by the fisheries will be dealt with. Furthermore, the GHG emissions for the unit weight of fishes are calculated with consideration to the different consuming areas as well. The results will be helpful to understand the circumstances of GHG emissions from Korean fisheries.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1262-1271
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• 2021

L-Malic acid (L-MA) is widely used in food and non-food products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). The objective of this work is to convert LB into L-MA with the concept of a bioeconomy and environmentally friendly process. The unique trifunctional xylanolytic enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6, effectively hydrolyzed xylan in untreated LB, especially corn hull to xylose, in one step. Furthermore, the newly isolated, Acetobacter tropicalis strain H1 was able to convert high concentrations of xylose derived from corn hull into L-MA as the main product, which can be easily purified. The strain H1 successfully produced a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. The process presented in this research is an efficient, low-cost and environmentally friendly biological process for the green production of L-MA from LB.

• Korean Chemical Engineering Research
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• v.59 no.4
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• pp.639-643
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• 2021

In this study, fluorinated ethylene propylene (FEP) nanoparticle as an adhesive for fabricating a three-dimensional multilayered microfluidic device was studied. The formation of evenly distributed FEP nanoparticles layer with 3 ㎛ in thickness on substrates was achieved by simple spin coating of FEP dispersion solution at 1500 rpm for 30 s. It is confirmed that FEP nanoparticles transformed into a hydrophobic thin film after thermal treatment at 300 ℃ for 1 hour, and fabricated polyimide film-based microfluidic device using FEP nanoparticle was endured pressure up to 2250 psi. Finally, a three-dimensional multilayered microfluidic device composed of 16 microreactors, which are difficult to fabricate with conventional photolithography, was successfully realized by simple one-step alignment of FEP coated nine polyimide films. The developed three-dimensional multilayered microfluidic device has the potential to be a powerful tool such as high-throughput screening, mass production, parallelization, and large-scale microfluidic integration for various applications in chemistry and biology.

• Animal Bioscience
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• v.34 no.4
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• pp.516-524
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• 2021

Objective: The genetic evaluation of Santa Inês sheep was performed for resistance to gastrointestinal nematode infection (RGNI) and body size using different relationship matrices to assess the efficiency of including genomic information in the analyses. Methods: There were 1,637 animals in the pedigree and 500, 980, and 980 records of RGNI, thoracic depth (TD), and rump height (RH), respectively. The genomic data consisted of 42,748 SNPs and 388 samples genotyped with the OvineSNP50 BeadChip. The (co)variance components were estimated in single- and multi-trait analyses using the numerator relationship matrix (A) and the hybrid matrix H, which blends A with the genomic relationship matrix (G). The BLUP and single-step genomic BLUP methods were used. The accuracies of estimated breeding values and Spearman rank correlation were also used to assess the feasibility of incorporating genomic information in the analyses. Results: The heritability estimates ranged from 0.11±0.07, for TD (in single-trait analysis using the A matrix), to 0.38±0.08, for RH (using the H matrix in multi-trait analysis). The estimates of genetic correlation ranged from -0.65±0.31 to 0.59±0.19, using A, and from -0.42±0.30 to 0.57±0.16 using H. The gains in accuracy of estimated breeding values ranged from 2.22% to 75.00% with the inclusion of genomic information in the analyses. Conclusion: The inclusion of genomic information will benefit the direct selection for the traits in this study, especially RGNI and TD. More information is necessary to improve the understanding on the genetic relationship between resistance to nematode infection and body size in Santa Inês sheep. The genetic evaluation for the evaluated traits was more efficient when genomic information was included in the analyses.