Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bioconversion of Untreated Corn Hull into L-Malic Acid by Trifunctional Xylanolytic Enzyme from Paenibacillus curdlanolyticus B-6 and Acetobacter tropicalis H-1

  • Duong, Thi Bich Huong (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Ketbot, Prattana (Excellent Center of Enzyme Technology and Microbial Utilization, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi) ;
  • Phitsuwan, Paripok (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Waeonukul, Rattiya (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Tachaapaikoon, Chakrit (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Kosugi, Akihiko (Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences) ;
  • Ratanakhanokchai, Khanok (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi) ;
  • Pason, Patthra (Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi)
  • Received : 2021.05.11
  • Accepted : 2021.07.13
  • Published : 2021.09.28
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Abstract

L-Malic acid (L-MA) is widely used in food and non-food products. However, few microorganisms have been able to efficiently produce L-MA from xylose derived from lignocellulosic biomass (LB). The objective of this work is to convert LB into L-MA with the concept of a bioeconomy and environmentally friendly process. The unique trifunctional xylanolytic enzyme, PcAxy43A from Paenibacillus curdlanolyticus B-6, effectively hydrolyzed xylan in untreated LB, especially corn hull to xylose, in one step. Furthermore, the newly isolated, Acetobacter tropicalis strain H1 was able to convert high concentrations of xylose derived from corn hull into L-MA as the main product, which can be easily purified. The strain H1 successfully produced a high L-MA titer of 77.09 g/l, with a yield of 0.77 g/g and a productivity of 0.64 g/l/h from the xylose derived from corn hull. The process presented in this research is an efficient, low-cost and environmentally friendly biological process for the green production of L-MA from LB.

Keywords

Acknowledgement

This research project is supported by King Mongkut's University of Technology Thonburi through the "KMUTT Research Center of Excellence Project (Grant no. 7601.24/4054)". The authors also acknowledge the financial support provided by Thailand Science Research and Innovation (TSRI) through the "Basic Research Fund. Duong Thi Bich Huong gratefully acknowledges the support provided by KMUTT through the "Petchra Pra Jom Klao Master Degree Research Scholarship".

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