• Title/Summary/Keyword: Producer

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A newly isolated Klebsiella pneumoniae producing a thermostable stereo-selective esterase for production of D-β-acetylthioisobutyric acid (D-β-Acetylthioisobutyric acid 생산을 위한 내열성 광학선택적 esterase 활성 Klebsiella pneumoniae의 분리)

  • Chung, Yong-Joon
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.143-148
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    • 2019
  • The synthesis of captopril as an important chiral drug in commerce needs expensive resolution process of racemic mixture. Microorganisms, producing a thermostable esterase that catalyzes the stereo-selective hydrolysis of methyl DL-${\beta}$-acetylthioisobutyrate (DL-ester) to D-${\beta}$-acetylthioisobutyric acid (DAT) were screened from soils. Among the strains tested, strain No CJ-317 and strain No CJ-187 with highest activity were selected as the best DAT producer. The newly isolated microorganisms were identified respectively, as Klebsiella pneumoniae and Pseudomonas putida. The cell activity of esterase from K. pneumoniae CJ-317 and P. putida CJ-187 were showed an optimal reaction activity at $75^{\circ}C$ and $60^{\circ}C$, respectively. Also the cell activity of K. pneumoniae CJ-317 was stable up to $80^{\circ}C$ for 1 h, while that of P. putida CJ-187 was not over $60^{\circ}C$. By varying the concentration of DAT in the reaction mixture, the cell activity of P. putida CJ-187 showed about 55% and 80% of product inhibition in the presence of 2.5% (w/v) and 5.0% of DAT respectively. K. pneumoniae CJ-317 had less product inhibition than P. putida CJ-187 by about 35% and 44% at the same concentrations respectively. The esterase of newly isolated K. pneumoniae CJ-317 could be useful for the stereo-selective hydrolysis of DL-ester to DAT.

Exploring the Selective Properties of buyer of Taekwon gymnastics for competition (대회용 태권체조 구매자의 선택속성 탐색)

  • Cho, Hee-Joo;Kim, Il-Gwang
    • Journal of Digital Convergence
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    • v.19 no.4
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    • pp.355-363
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    • 2021
  • The purpose of this research is to explore the selection attributes that the director of a taekwondo gym places importance on when purchasing a work of taekwon gymnastics for the competition. We conducted in-depth interviews with 6 participants using qualitative case study techniques, and analyzed data using Creswell's (2015) spiral analysis method. The data analysis results show music, trainees, characteristics of the work, criteria for evaluating the competition, producer competence, and price factors. As a result of the research, music should be produced around diverse and differentiated genres and music preferred by the trainees, and the appropriate theme should be selected considering the expressiveness and level of the trainees. The work should be considered creative works that harmonize the movement ratio of Taekwondo movements and dance, and the length of the work should be adjusted according to the purpose of the buyer, and the construction of the work should be based on the regulations of the competition. The manufacturer should ensure smooth communication with the buyer, including coaching ability and experience at the convention site. Prices will be affordable and affordable, and prices will need to be calculated based on special prices or mentoring periods for a small number of buyers.

The Facets of Photographic Records on Korea in Modern Era (조선말과 대한제국 시기 사진기록물의 성격과 생산, 유통 과정)

  • Park, Ju Seok
    • The Korean Journal of Archival Studies
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    • no.62
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    • pp.225-258
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    • 2019
  • Since the opening of Joseon in 1876, the photographic records of the late Joseon Dynasty and the period of the Korean Empire exist more than expected, considering the technological level and the social situations at that time. Photographs related to Korea can be distributed in various forms, such as illustrations of books printed to introduce Korea to Western society, plates of graphic journalism like newspapers or magazines, vintage prints, photo-postcards, stereo-photographs, card-type photographs, and lantern slides. There are still a great deal left in various archives of the Europe, America, Japan and Korea. According to related researchers, Korean-related photographs taken between 1863 and 1910, since Koreans were first photographed, were at least 3,000 to 4,000 cuts and the photo postcards issued was 25,000. It is said that most of them exist. This paper categorizes two ways of producing and distributing photographic records related to Korea, which were early modern times. The subjects of the photographs are clearly Korea or Koreans, but most of the producers of these photographs were Westerners and Japanese, who were imperial servants of imperialism. In the case of photography, there is a great possibility of distortion of the facts depending on the needs or perspectives of the producers. In order to correct the distortion, not only the contents of the photograph but also the intention of the producer, the production and the communication status should be grasped. This is because the problem of reading photograph records accurately and fairly in an age where there is no real experience is the cornerstone for understanding modern Korea correctly and broadly studying the Modern History of Korea.

A Comparative Study of Block Chain : Bitcoin·Namecoin·MediBloc (블록체인 비교연구: 비트코인·네임코인·메디블록)

  • Kim, Ji Yeon
    • Journal of Science and Technology Studies
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    • v.18 no.3
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    • pp.217-255
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    • 2018
  • Bitcoin, which appeared in 2008, was merely a conceptual virtual currency, but it now enjoys the status as actual money. Bitcoin is an electronic money system that can be traded directly without a central trust institution. Thanks to the popularization of Bitcoin, blockchain technology has become a widespread concern. That technology is expanding not only the currency mechanism, but also a variety of other services. The possibility of a blockchain in relation to actual currency is ongoing. This paper investigates the technological characteristics and social construction of the blockchain by comparing the cases of Bitcoin, Namecoin, and MediBloc among blockchain applications. Namecoin emerged in 2013 is an attempt to replace the centralized Internet Domain Name System(DNS). There has been controversy over that current system for a long time, but replacing the already established system is not easy. Nevertheless, Namecoin has potential as an alternative. Meanwhile, MediBloc is an application that involves distributed management of medical data in South Korea. MediBloc claims that the key producers of medical data are patients themselves. This is to challenge to the question who is a knowledge producer of medical data. Through these three cases, it has discussed that blockchain technology does supports to form more democratic decision-making or simply provide a technical solution as automation. As a citizen, we can intervene in the realization of blockchains by presenting social agenda. This will be a method of the social construction of technology.

A Study of Communication Factor in the Chinese augury bone (은상(殷商)시기 갑골문(甲骨文)에 나타난 커뮤니케이션 속성(屬性))

  • Lee, Bum-Soo
    • (The)Study of the Eastern Classic
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    • no.43
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    • pp.305-328
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    • 2011
  • This study examines a study of communication factor in the Chinese augury bone, as a communication text, a communication history text and a communication thought text, in terms of sender message channel audience effects, interdisciplinary research. In many respects, it is generally accepted that the Chinese augury bone have been the generic references of the Oriental culture. Based on various research results about The Chinese augury boney as a communication text, this paper explores factors of communication text related with meta-communication as the definition of communication and a structure of communication, a method of communication. The Chinese augury bone consider graphic element as communication tool, its manager as sender or communicator, the record as message, the bone as channel, its reader as audience, text understanding and behavior as effects, the augury bone producer as journalist, the tying bone in a bundle as old style book. In the Chinese augury bone, one property of communication thought is that the political ideology, which is related to the strong and weak of the graphic style and determines the naming method of kings.

Optimization of Medium to Improve Protease Production Using Response Surface Methodology by Bacillus amyloliquefaciens SRCM115785 (반응표면분석법을 이용한 Bacillus amyloliquefaciens SRCM115785의 protease 활성증가를 위한 배지 최적화)

  • Yang, Hee Gun;Ha, Gwangsu;Ryu, Myeong Seon;Park, Se Won;Jeong, Ho Jin;Yang, Hee-Jong;Jeong, Do-Youn
    • Journal of Life Science
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    • v.31 no.8
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    • pp.761-770
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    • 2021
  • In this study, the optimal medium composition for enhancing protease production was established by the Bacillus strain isolated from Makgeolli, a traditional fermented food, using the response surface methodology. B. amyloliquefaciens SRCM115785 was selected as the protease producer by productivity analysis and identified by 16S rRNA gene sequencing. Plackett-Burman design (PBD) was introduced to analyze the effect of each component on protease production among the 11 selected medium components. As a result, glucose, yeast extract, and beef extract were finally selected as factors for enhancing protease production. Central composite design (CCD) analysis was designed as a method to determine the optimal concentration of each component for protease production and the concentration of each medium composition for maximum protease production was predicted to glucose 6.75 g/l, yeast extract 12.42 g/l and beef extract 17.48 g/l. The suitability of the experimental model was proved using ANOVA analysis and as a result of quantitative analysis to prove this, the amount of increase was 230.47% compared to the LB medium used as a control. Through this study, the optimization of medium composition for enhancing protease production was established, and based on this, it is expected that it can be efficient use of protease as an industrial enzyme.

A study on frost prediction model using machine learning (머신러닝을 사용한 서리 예측 연구)

  • Kim, Hyojeoung;Kim, Sahm
    • The Korean Journal of Applied Statistics
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    • v.35 no.4
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    • pp.543-552
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    • 2022
  • When frost occurs, crops are directly damaged. When crops come into contact with low temperatures, tissues freeze, which hardens and destroys the cell membranes or chloroplasts, or dry cells to death. In July 2020, a sudden sub-zero weather and frost hit the Minas Gerais state of Brazil, the world's largest coffee producer, damaging about 30% of local coffee trees. As a result, coffee prices have risen significantly due to the damage, and farmers with severe damage can produce coffee only after three years for crops to recover, which is expected to cause long-term damage. In this paper, we tried to predict frost using frost generation data and weather observation data provided by the Korea Meteorological Administration to prevent severe frost. A model was constructed by reflecting weather factors such as wind speed, temperature, humidity, precipitation, and cloudiness. Using XGB(eXtreme Gradient Boosting), SVM(Support Vector Machine), Random Forest, and MLP(Multi Layer perceptron) models, various hyper parameters were applied as training data to select the best model for each model. Finally, the results were evaluated as accuracy(acc) and CSI(Critical Success Index) in test data. XGB was the best model compared to other models with 90.4% ac and 64.4% CSI, followed by SVM with 89.7% ac and 61.2% CSI. Random Forest and MLP showed similar performance with about 89% ac and about 60% CSI.

A Study on Forecasting Industrial Land Considering Leading Economic Variable Using ARIMA-X (선행경제변수를 고려한 산업용지 수요예측 방법 연구)

  • Byun, Tae-Geun;Jang, Cheol-Soon;Kim, Seok-Yun;Choi, Sung-Hwan;Lee, Sang-Ho
    • The Journal of the Korea Contents Association
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    • v.22 no.1
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    • pp.214-223
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    • 2022
  • The purpose of this study is to present a new industrial land demand prediction method that can consider external economic factors. The analysis model used ARIMA-X, which can consider exogenous variables. Exogenous variables are composed of macroeconomic variable, Business Survey Index, and Composite Economic Index variables to reflect the economic and industrial structure. And, among the exogenous variables, only variables that precede the supply of industrial land are used for prediction. Variables with precedence in the supply of industrial land were found to be import, private and government consumption expenditure, total capital formation, economic sentiment index, producer's shipment index, machinery for domestic demand and composite leading index. As a result of estimating the ARIMA-X model using these variables, the ARIMA-X(1,1,0) model including only the import was found to be statistically significant. The industrial land demand forecast predicted the industrial land from 2021 to 2030 by reflecting the scenario of change in import. As a result, the future demand for industrial land was predicted to increase by 1.91% annually to 1,030.79 km2. As a result of comparing these results with the existing exponential smoothing method, the results of this study were found to be more suitable than the existing models. It is expected to b available as a new industrial land forecasting model.

Social Network Analysis(SNA)-Based Korean Film Producer-Director-Actor Network Analysis : Focusing on Films Released Between 2013 and 2019 (한국영화 제작자·감독·배우 네트워크 분석: 2013~2019년 개봉작 중심으로)

  • Cho, Hee-Young
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.4
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    • pp.169-186
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    • 2020
  • This study selected 127 powerful Korean film producers, directors, and actors whose stable audience drawing power has been proven over the past seven years from 2013 to 2019, and viewed their network through social network analysis(SNA) to explain their power structure. It also explained the changes compared to the results of previous studies conducted on box office hits from 1998 to 2012. The producers who showed the highest audience drawing power over the past seven years were KANG Hae-jung, JANG Won-seok, LEE Eugene, HAN Jae-duk. BONG Joon-ho, KIM Yong-hwa, and RYOO Seung-wan as directors and SONG Kang-ho, HA Jung-woo, and HWANG Jung-min as actors were confirmed to exhibit the most stable audience drawing power. Meanwhile, the network formed by the 127 leading producers, filmmakers, and actors was analyzed based on closeness/ degree/eigenvector/betwenness centrality, and the result discovered a strong network involving JANG Won-seok, HAN Jae-duk, CHO Jin-woong, Don LEE, and HWANG Jung-min. This study is meaningful in that it included producers, the position which has never been discussed in previous local studies to analyze the network influencing star casting, and selected accurate box office hits by checking whether the concerned films actually reached break-even point rather than simply relying on the number of audiences or total revenue they garnered. Nonetheless, it left a hole to be filled in that it did not include the role of the management companies in the network. Therefore, a relevant follow-up discussion would be needed.

Analysis of the Global Fandom and Success Factors of BTS (방탄소년단(BTS)의 글로벌 팬덤과 성공요인 분석)

  • Yoon, Yeo-Kwang
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.3
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    • pp.13-25
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    • 2019
  • Since reaching the top in the Billboard Main Album Chart 'Billboard 200' with Love Yourself: Tear in May of 2018, BTS once again took first place after just three months in the 'Billboard 200'(September 3, 2018) with the repackaged album Love Yourself: Answer. It opened the doors to the 'Hallyu 4.0' by conquering the main Billboard Chart with a song sung in Korean. BTS rose to the top on the 'Billboard 200' twice, thus being recognized globally for their musical talent(song, dance, promotion, etc.), and took their place in the mainstream music market of the world. BTS moved away from intuitive interaction such as mysticism, abnormality, irregularity, etc. but instead created their own world(BTS Universe) with fans around the world through two-directional communication such as consensus, sharing and co-existence. They are recognized as artists that went beyond being an idol group that simply released a few hit songs that had now elevated popular music to a new form of art. In result, they retained a highly loyal global fan base(A.R.M.Y.) and they are continuously creating good influence with them. This study analyzed the success factors of BTS using the S-M-C-R-E model as follows. ① Sender: BTS'7-person 7-colors fantasy and 'All-in-one storytelling' strategy of producer Bang Shi-hyuk ② Message: Create global consensus of 'you' rather than 'me' ③ Channel: Created real-time common grounds with global fans through social network platforms such as Youtube, Facebook and Instagram ④ Receiver: Formed highly loyal global fandom(A.R.M.Y.) that extends outside of Korea and Asia ⑤ Effect: Created additional economic value and spread good influence