• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.3 s.258
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• pp.380-387
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• 2007

We carried out an image analysis of living cells forming their contacts at the bottom of the cell culturing substrate. In order to visualize the contact area selectively, we adopted total-internal-reflection-fluorescence (TIRF) method, which can illuminate the specimen volume within only several hundred nano-meters above the substrate. From the fluorescent intensity of the TRF image, we could calculate the distance of the cell surface from the substrate. As a result, we visualized the origin of cell contacts, their movements, and the change of cell-contact type from the close-contact into focal-contact with information of its vertical displacement representing the temporal evolution process of the three-dimensional cell-surface-profile near the contact area during this metamorphosis.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.3
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• pp.77-85
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• 2015

One of the key factors of early development is the specification of competence between the oocyte and the sperm, which occurs during gametogenesis. However, the starting point, growth, and maturation for acquiring competence during spermatogenesis and oogenesis in mammals are very different. Spermatogenesis includes spermiogenesis, but such a metamorphosis is not observed during oogenesis. Glycosylation, a ubiquitous modification, is a preliminary requisite for distribution of the structural and functional components of spermatids for metamorphosis. In addition, glycosylation using epididymal or female genital secretory glycans is an important process for the sperm maturation, the acquisition of the potential for fertilization, and the acceleration of early embryo development. However, nonemzymatic unexpected covalent bonding of a carbohydrate and malglycosylation can result in falling fertility rates as shown in the diabetic male. So far, glycosylation during spermatogenesis and the dynamics of the plasma membrane in the process of capacitation and fertilization have been evaluated, and a powerful role of glycosylation in spermatogenesis and early development is also suggested by structural bioinformatics, functional genomics, and functional proteomics. Further understanding of glycosylation is needed to provide a better understanding of fertilization and embryo development and for the development of new diagnostic and therapeutic tools for infertility.

• The Korean Journal of Zoology
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• v.27 no.3
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• pp.165-176
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• 1984

The cutaneous erythrophore differentiation from larvae to adult in bombina orientalis-the Korean fire bellied toad-is discussed electron microscopically to reveal the structural changes of carotenoid vesicles and pterinosomes in the process of erythrophore differentiation. Typical structure of adult erythrophores distributed beneath the basement membrane is composed much carotenoid vesicles and a small quantity of pterinosomes. Marginal electron density of these carotenoid vesicles is higher than those of internal regions and pterinosomes have many concentric lamellae. The very first differentiated erythrophores are apeared before and after the foreleg developing stage of metamorphosis and red ventral color is just appeared about $1\\sim2$ weeks after metamorphosis. About 1 year after metamorphosis, external body color of the young toad is similar to adult but the morphology of pigment granules observed in these erythrophores differs from that of adult in structure and electron density. At this stage, the differentiated level of pterinosomes is not remarkable and the accumulation state of carotenoid pigments in erythrophores is also relatively low.

• The Korean Journal of Zoology
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• v.28 no.3
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• pp.151-165
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• 1985

The morphological changes of the midgut epithelium during the metamorphosis of pine caterpillar are observed with light and electron microscope, being divided into 5 stages from the 8th instar larva to just after pupation. The midgut epithelium of 8th instar larva is composed of columnar cell, goblet cell, regenerative cell, and endocrine cell. The secretorials are arranged on the nuclear membrane in the columnar cell of the midgut epithelium in the 8th instar larva, and lysosomes are augmented in the apical portion. Cytoplasmic extrusions are observed in the apical surface of columnar cell but they have no cell organells. Nucleus, mitochondria, rER, Golgi complex, and free ribsomes are observed in the regenerative cell. Regenerative cells are differentiated into the form of goblet cell, and vacuoles are gradually increased in the cytoplasm. Just pupa stage, the materials, which appears to be mainly composed of Ca, are observed in the circular form and goblet cavity of regenerative cell are detached to lumen. As a result, it reflects the process of the degeneration of the midgut epithelium that lysosomes are gradually augmented in the columnar cell, that nuclear materials are removed to cytoplasm, and that cytoplasmic extrusions are observed in the apical surface. And though regenerative cells are differentiated into the form of the goblet cell, it is believed that goblet cavity is detached from regenerative cell to the lumen and midgut epithelium of pupa stage is formed.

• Journal of Environmental Health Sciences
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• v.28 no.4
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• pp.17-22
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• 2002

In crustaceans, as in other arthropods, the molt cycle and the physiological process of growth are controlled by molting hormones (MH) which are steroid hormones, the ecdysteroids. Ecdysteroids are major arthropod hormones which control both development (embryonic and larval molts, metamorphosis) and reproduction. The purpose of the present study was to evaluate both fenarimol and methoprene for embryotoxicity to daphnids. The embryotoxicity associated with each compound was assessed to discern whether the embryotoxicity of methoprene might be due to ecdysone agonist and the ecdysone antagonistic effects of fenarimol on Daphnia embryo. Exposure of daphnids for three weeks to 50 M methoprene resulted in a significantly high incidence of offspring that exhibited general toxicity. This exposure concentration had significant effects on the overall number of embryo death. However, exposure to 3 or 1 $\mu$M fenarimol were no significant effects on the embryo toxicity. The incidence of both of these toxicity increased with methoprene exposure. This observation suggest that methoprene showed embryonic general toxicity during embryo development, while, only fenarimol showed weak general toxicity with early stages of embryonic development.

• Proceeding of KASS Symposium
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• 2006.05a
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• pp.142-152
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• 2006

The purpose of this study is to explore an inquisitive and innovative approach to structure and form, Calatrava's morphogenetic mechanism and to trace the ideas behind his working methods and his theoretical preoccupations. The bridges and buildings of S. Calatrava possess a breathtaking rhythm and of them some are designed to expand and contract like living organisms. The analogy Calatrava has used as a creative tool to mutate human bodies into arcing roof forms and bridge suspensions is introduced to illustrate the morphogenetic process. At the same time, the analysis used developing how to design frame structures foldable with brilliant mathematical solution is also investigated. Consequently, the potentialities of Calatrava's morphogenetic mechanism to invent new systems are generated by methodological hypothesis; analysis and analogy.

• Korean Journal of Ecology and Environment
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• v.37 no.1 s.106
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• pp.96-101
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• 2004

The effects of the ecdysteroid agonist tebufenozide on the larvae of Chironomus flaviplumus and Chironomus riparius were tested in the laboratory. Employing a static exposure setup, chironomids were subjected to various tebufenozide concentrations. In the most treatments it reach-ed a statistically significant difference from the control condition. As the concentration of tebufenozide was increased, a relatively larger proportion of the observed mortality was associated with the metamorphosis and molting process. The larval mortality of C. riparius was high in C. flaviplumus during over 30 ${\mu}g\;L^{-1}$ treatments. In terms of development, the effects of tebufenozide were delayed growth stage in relatively lower concentration such as 10 ${\mu}g\;L^{-1}$ tebufenozide treatments. The rates of succeed adult through the molting process were various in treated concentrations or/and the species.

• Journal of Korean Elementary Science Education
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• v.25 no.4
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• pp.352-362
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• 2006

Many misconceptions regarding biology and evolution have been reported by students prior to being exposed to a formal education program of evolution which challenged them. This study sought to investigate and to analyze the misconception formation process of evolution originating from TV animation and science books for children. Firstly, to identify TV animation's influence on students' misconceptions of evolution, a questionnaire including TV animation characters was constructed and administered to 146 elementary school students, 161 middle school students, and 156 high school students. The data collected was analyzed. Secondly, 17 science books for children were sampled and the contents related to evolution were selected and analyzed in terms of five evolutionary explanations: creationism internal will explanation, teleological explanations, explanations of use and disuse, mutation and finally, natural selection. Children have understood 'growth' and 'metamorphosis' on TV animation as 'evolution'. The processes by which characters on TV animation undergo some forms of change, which are in fact a kind of metamorphosis has often been understood as 'evolution'. Many respondents have defined evolution incorrectly as the process of growing and changing shape. On the other hand, some science books fur children contained descriptions of evolution including' mutation and finally natural selection explanation'; however, most of the science books fur children sampled in this study were written through the perspectives of alternative evolutionary views such as 'teleology view', 'internal will view', and 'use and disuse view'. It is apparent that TV animation and science books fur children influence the formation of various misconceptions regarding evolution by children.

• Journal of Energy Engineering
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• v.27 no.3
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• pp.48-56
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• 2018

The shell waste biomineralization process has known a tremendous metamorphosis and also the nanostructure with the identification of matrix proteins in oyster shells. However, proteins are represented in minor shell components and they are the major macromolecules that control biocrystal synthesis. Aragonite and calcite were derived from molluscan shells and evaluated the source of carbonate minerals and it helps for the formation of limestone. The oyster shell wastes are large and massive. The paleoecological study of oyster beds has discovered a near-shore and thin Upper Rudeis formation with storm influence during the accumulation of oysters with highly altered by disarticulation, bioerosion, and encrustation. It is possible even in the Paleozoic mollusks provided sufficient carbonate entirely to the source of microcrystalline of limestone. The present review is to discuss paleoecologically a number of oyster shell beds accumulated and sediment to form the different types of limestone during the Middle Miocene time.

• Korean Institute of Interior Design Journal
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• v.15 no.5 s.58
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• pp.60-67
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• 2006

An architecture is a result from representation of design knowledge by architect's creative thinking, and operated only in his internal organization system. Design knowledge based upon the experience that they have achieved in the past as well as the informations they have accumulated over the time. In this point of view, the Schema that formed by problems settlement and process repetitions for the best conclusion, not only play an important part in the designer's thinking system about a specific problem but also appeared by distinctive features. This research looks to achieve a new design method by finding out how architects express traditionality in the contemporary house through schema. This research analyze contemporary house built in seoul of 1990's. The result are as followings. First, Schema is a 'frame' that already exist in the architect's thinking, will make a good guide about express the traditionality. Second, We found out several distinctive characters in the form and the space. The form is divided in five types. It is an condensed form of roof image, the metamorphosis of form grammar, the change of part details, the representation of design principle and the directly expression. Lastly, In the space, it is the borrow of the connection method of inside and external space, the three parts composition of the space, the adaptation of 'Madang' and the Independent composition of the space due to 'Chae'