• Proceedings of the Korean Society of Crop Science Conference
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• 2021.04a
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• pp.113-113
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• 2021

• Journal of Microbiology
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• v.45 no.3
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• pp.219-226
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• 2007

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCA GGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.

• Molecules and Cells
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• v.27 no.1
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• pp.21-37
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• 2009

Map-based cloning to find genes of interest, marker-assisted selection (MAS), and marker-assisted breeding (MAB) all require good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences, 13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830 markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained 169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes. The integrated map contained four genetic maps of two interspecific (Capsicum annuum 'TF68' and C. chinense 'Habanero') and two intraspecific (C. annuum 'CM334' and C. annuum 'Chilsungcho') populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM) in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were first developed from end sequences of BAC clones from Capsicum annuum 'CM334'. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment of linkage groups to pepper chromosomes.

• The Plant Pathology Journal
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• v.26 no.2
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• pp.170-177
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• 2010

Bacillus licheniformis N1, previously developed as a biofungicide formulation N1E to control gray mold disease of plants, was investigated to study the bacterial traits that may be involved in its biological control activity. Two N1E based formulations, bacterial cell based formulation PN1E and culture supernatant based formulation SN1E, were evaluated for disease control activity against gray mold disease of tomato and strawberry plants. Neither PN1E nor SN1E was as effective as the original formulation N1E. Fractionation of antifungal compounds from the bacterial culture supernatant of B. licheniformis N1 indicated that two different cyclic lipopeptides were responsible for the antimicrobial activity of the N1 strain. These two purified compounds were identified as iturin A and surfactin by HPLC and LCMS. The purified lipopeptides were evaluated for plant disease control activity against seven plant diseases. Crude extracts and purified compounds applied at 500 ${\mu}g/ml$ concentration controlled tomato gray mold, tomato late blight and pepper anthracnose effectively with over 70% disease control value. While iturin showed broad spectrum activity against all tested plant diseases, the control activity by surfactin was limited to tomato gray mold, tomato late blight, and pepper anthracnose. Although antifungal compounds from B. licheniformis N1 exhibited disease control activity, our results suggested that bacterial cells present in the N1E formulation also contribute to the disease control activity together with the antifungal compounds.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.32-32
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• 2018

Flora investigations in Korea have been conducted by many researchers for diverse purposes. Accumulated flora investigation data has not been utilized efficiently because there is no accessible database for comparison. To overcome this shortcoming, we constructed web-based database of flora investigation, named as the Integrated Korean Flora Database (IKFD; http://www.floradb.net/intro.php). Until now, 284 flora references (263 papers, 14 reports and books, and 7 unpublished papers written in between 1962 and 2017) were digitalized into the database. From 134,711 records, 4,301 species belonging to 228 families and 1,079 genera were identified via mapping with two major Korean plant species lists. Polygon areas originated from references were used for distribution of plant species, identifying precise distribution area. It will be a better index to show plant ecological characteristics. Collected micro-climate data provided by Korea Meteorology Administration were also integrated in IFKD for understanding correlation between distribution of plants and micro-climate. Cold hardiness zone which has been utilized for classifying climate zones. 12 out of 26 zones identified based on micro-climate data in Korea were mapped with distribution of plants. More than half species were appeared in zone 6a, 6b, 7a, and 7b. Taken together with these results, IKFD will be a fundamental platform for understanding plants in Korea flora investigation as well as a new standard for classifying distribution of plants. Moreover, Biodiversity Observation Database (BODB; http://www.biodiversitydb.info/intro.php) which integrates plant distribution data was also integrated for further studies.

• Journal of the Korean Society of Systems Engineering
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• v.15 no.1
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• pp.34-42
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• 2019

Receiving great attention of IoT and 4th industrial revolution, the necessity comes to the fore of the plant system which aims making it smart and effective. Smart Factory is the key realm of IoT to apply with the concept to optimize the entire process and it presents a new and flexible production paradigm based on the collected data from numerous sensors installed in a plant. Especially, the wireless sensor network technology is receiving attention as the key technology of Smart Factory, researches to interface those technology is actively in progress. In addition, IoT devices for plant industry security and high reliable network protocols are under development to cope with high-risk plant facilities. In the meanwhile, Blockchain can support high security and reliability because of the hash and hash algorithm in its core structure and transaction as well as the shared ledger among all nodes and immutability of data. With the reason, this research presents Blockchain as a method to preserve security and reliability of the wireless communication technology. In regard to that, it establishes some of key concepts of the possibility on the blockchain based IoT Edge devices for Plant O&M (Operations and Maintenance), and fulfills performance verification with test devices to present key indicator data such as transaction elapsed time and CPU consumption rate.

• Research in Plant Disease
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• v.22 no.4
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• pp.243-248
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• 2016

As preventive measures for bonsai exports, nematodes were isolated from 55 bonsai samples of five coniferous species (Chamaecyparis pisifera, Juniperus chinensis, Pinus densiflora, Pinus thunbergii, and Taxus cuspidate) from all 15 bonsai gardens in Korea. Nematodes belonging to 21 genera in 7 orders were isolated from the 55 bonsai samples. Among plant-parasitic nematodes, Tylenchus spp. was the most frequently isolated (14.9%), followed by Ditylenchus spp. (10.5%), Aphelenchoides spp. (9.5%), Aphelenchus sp. (5.5%), Criconemoides sp. (4.0%), Helicotylenchus sp. (0.7%), Hemicycliophora sp. (0.7%), Mesocriconema sp. (0.7%), Tylenchorhynchus sp. (0.7%), and Paratylenchus sp. (0.4%). Among nonparasitic nematodes, Cephalobina was the most frequently isolated nematodes (26.5%), followed by Rhabditida (19.3%), Dorylaimida (17.8%), Pangrolaimida (14.5%), Plectida (6.5%), Tryphylida (6.2%), Mononchida (3.3%), Alaimida (2.9%), Monhysterida (2.5%), and Triplonchida (0.4%). Based on these results, we conclude that there is no problematic plant-parasitic nematode in bonsai gardens of Korea.

• 한국신재생에너지학회:학술대회논문집
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• 2009.11a
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• pp.325-328
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• 2009

The 10t/d pyrolysis demonstration plant have been developed for waste tire recycling treatment and value added commercialization. The initial plant model had been started under 2.4t/d capacity with continuous operation, and the commercial plant has been achieved to the 120t/d based on demonstration plant having the tube reactor with chain conveyer attached disk. The next generation pyrolysis plant for waste tire is reviewed and the assignment for plant development is presented briefly.

• Journal of Microbiology and Biotechnology
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• v.34 no.8
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• pp.1563-1579
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• 2024

More and more diterpenoids have attracted extensive attention due to the diverse chemical structures and excellent biological activities, and have been developed into clinical drugs or consumer products. The vast majority of diterpenoids are derived from plants. With the long-term development of plant medicinal materials, the natural resources of many plant diterpenoids are decreasing, and the biosynthetic mechanism of key active components has increasingly become a research hotspot. Using synthetic biology to engineer microorganisms into "cell factories" to produce the desired compounds is an essential means to solve these problems. In this review, we depict the plant-derived diterpenoids from chemical structure, biological activities, and biosynthetic pathways. We use representative plant diterpenes as examples to expound the research progress on their biosynthesis, and summarize the heterologous production of plant diterpenoids in microorganisms in recent years, hoping to lay the foundation for the development and application of plant diterpenoids in the future.

• The Plant Pathology Journal
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• v.34 no.6
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• pp.514-531
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• 2018

Tomato spotted wilt virus (TSWV; Genus Orthotospovirus: Family Tospoviridae) is one of the most destructive viruses affecting a wide range of horticultural crops on a worldwide basis. In 2015 and 2016, 171 leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RT-PCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based on the nucleocapsid (N) and non-structural (NSs) genes, respectively. The amplified fragments were cloned into pTG19-T and sequenced. Sequence comparisons with those available in the GenBank showed that the sequences belong to TSWV. The high nucleotide identity and similarities of new sequences based on the L, N, and NSs genes showed that minor evolutionary differences exist amongst the isolates. The phylogenetic tree grouped all isolates six clades based on N and NSs genes. Phylogenetic analysis showed that the Iranian isolates were composed a new distinct clade based on a part of polymerase, N and NSs genes. To our knowledge, this is the first detailed study on molecular characterization and genetic diversity of TSWV isolates from tomato in Iran that could be known as new clade of TSWV isolates.