• Title/Summary/Keyword: Pigment concentration

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HPLC Analysis of Biomass and Community Composition of Microphytobenthos in the Saemankeum Tidal flat, West Coast of Korea (한국 서해 새만금 갯벌에서 저서미세조류의 생체량과 군집조성에 대한 HPLC 분석)

  • OH Seung-Jin;MOON Chang-Ho;PARK Mi-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.3
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    • pp.215-225
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    • 2004
  • Biomass and community composition of microphytobenthos in the Saemankeum tidal flat were studied by HPLC analysis of the photosynthetic pigments from November 2001 to November 2002. The environmental factors of sediment were also investigated to examine the relationship between microphytobenthos biomass and sedimentary environments. The detected photosynthetic pigments of microphytobenthos were chlorophyll a, b, c, fucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, diadinoxanthin, alloxanthin, diatoxanthin, zeaxanthin+lutein, peridinin and beta-carotene. Pheophytin a, the degradation product of chlorophyll a, was also detected. The results of pigmen analysis suggest the presence of diatom (fucoxanthin), euglenophytes (chlorophyll b), chlorophytes (chlorophyll b + lutein), cyanobacteria (zeaxanthin), cryptophytes (alloxanthin), chrysophytes (fucoxanthin + violaxanthin), prymnesiophytes (19'-hexanoyloxyfucoxanthin) and dinoflagellates (peridinin). Chlorophyll a concentration in the top 0.5 cm of sediment was in the range of $0.24\;mg{\cdot}m\^{-2}\;-32.11\;mg{\cdot}m\^{-2}$ in the study area. The increase of chlorophyll a concentration in the spring indicates the occurrence of a microphytobenthic bloom. In the summer, there was a sharp decrease of the chlorophyll a concentration which was probably due to high grazing activity by macrobenthos. The annual mean chlorophyll a concentration in the study area was low compared to that in most of other tidal flat areas probably due to active resuspension of microphytobenthos and high grazing activity by macrobenthos. There was no clear relationship between microphytobenthos biomass and sedimentary environments because of a large variety of physical, chemical and biological factors, Pigment analysis indicated that while diatoms were dominated in the microphytobenthic community of the Geojon tidal flat, euglenophytes and/or chlorophytes coexisted with diatoms in the Mangyung River tidal flat.

A Study on the Correlation between the Harmful Cyanobacterial Density and Phycocyanin Concentration at Recreational Sites in Nakdong River (낙동강 친수활동구간 유해 남조류 분포와 피코시아닌(Phycocyanin) 농도 상관성에 관한 연구)

  • Hyo-Jin Kim;Min-Kyeong Kim
    • Journal of Korean Society on Water Environment
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    • v.39 no.6
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    • pp.451-464
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    • 2023
  • Harmful cyanobacterial monitoring is time-consuming and requires skilled professionals. Recently, Phycocyanin, the accessory pigment unique to freshwater cyanobacteria, has been proposed as an indicator for the presence of cyanobacteria, with the advantage of rapid and simple measurement. The purpose of this research was to evaluate the correlation between the harmful cyanobacterial cell density and the concentration of phycocyanin and to consider how to use the real-time water quality monitoring system for algae bloom monitoring. In the downstream of the Nakdong River, Microcystis spp. showed maximum cell density (99 %) in harmful cyanobacteria (four target genera). A strong correlation between phycocyanin(measured in the laboratory) concentrations and harmful cyanobacterial cell density was observed (r = 0.90, p < 0.001), while a weaker relationship (r = 0.65, p < 0.001) resulted between chlorophyll a concentration and harmful cyanobacterial cell density. As a result of comparing the phycocyanin concentration (measured in submersible fluorescence sensor) and harmful cyanobacterial cell density, the error range increased as the number of cyanobacteria cells increased. Before opening the estuary bank, the diurnal variations of phycocyanin concentrations did not mix by depth, and in the case of the surface layer, a pattern of increase and decrease over time was shown. This study is the result of analysis when Microcystis spp. is dominant in downstream of Nakdong River in summer, therefore the correlation between the harmful cyanobacteria density and phycocyanin concentrations should be more generalized through spatio-temporal expansion.

Temporal and Spatial Variation of Microalgal Biomass and Community Structure in Seawater and Surface Sediment of the Gomso Bay as Determined by Chemotaxonomic Analysis (색소분석을 통한 곰소만 내 해수와 퇴적물 중 미세조류 생체량과 군집구조의 시공간적 변화)

  • Lee, Yong-Woo;Park, Mi-Ok;Yoon, Ji-Hyun;Hur, Sung-Bum
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.2
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    • pp.87-94
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    • 2012
  • To compare monthly variations of phytoplankton biomass and community composition between in seawater and sediment of the Gomso Bay (tidal flat: approximately 75%), the photosynthetic pigments were analyzed by HPLC every month in 1999 and every two months in 2000. Ambient physical and chemical parameters (temperature, salinity, nutrients, dissolved oxygen, and chemical oxygen demand) were also examined to find the environmental factors controlling structure of phytoplankton community. The temporal and spatial variations of chlorophyll a concentration in seawater were correlated well with the magnitude of freshwater discharge from land. The biomass of microphytobenthos at the surface sediments was lower than that in other regions of the world and 2-3 times lower than phytoplankton biomass integrated in the seawater column. Based on the results of HPLC pigment analysis, fucoxanthin, a marker pigment of diatoms, was the most prominent pigment and highly correlated with chlorophyll a in seawater and sediment of the Gomso Bay. These results suggest that diatoms are the predominant phytoplankton in seawater and sediment of the Gomso Bay. However, the monthly variation of chlorophyll a concentration in seawater at the subtidal zone was not a good correlation with that in sediment of the Gomso Bay. Although pelagic plankton was identified in seawater by microscopic examination, benthic algal species were not found in the seawater. These results suggest that contribution from the suspended microphytobenthos in the tidal flat to the subtidal zone of the Gomso Bay may be low as a food source to the primary consumer in the upper water column of the subtidal zone. Further study needs to elucidate the vertical and horizontal transport magnitude of the suspended microphytobenthos in the tidal flat to the subtidal zone.

Lipid Oxidation in Red and Poultry Meats

  • Rhee Choi, Ki-Soon
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2003.06a
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    • pp.1-14
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    • 2003
  • Lipid oxidation is one of the most important non-microbial causes of meat quality deterioration. However, there have been different/conflicting views concerning the primary catalysts of lipid oxidation in meat. This presentation provides brief overviews of lipid oxidation mechanism in general and catalysis of lipid oxidation in meat, and then focuses on inter-species differences in lipid oxidation potential, using results from our studies on meats (beef, pork and chicken) at retail and the respective meats of uniform postmortem history. The inter-species differences have highlighted the relative roles of meat pigment (myoglobin) content, catalase activity, and the concentration of oxidation substrates (particularly polyunsaturated fatty acids) in determining the lipid oxidation potential of raw meat versus cooked meat.

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Mycelial Growth Enhancement in Liquid Cultivation of Hericium erinaceus

  • Kim, Min-Seok;Seo, Jeong-Sik;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.236-239
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    • 2003
  • Erinacine which is produced from Hericium erinaceus mycelia is known to have a stimulating activity of nerve growth factor(NGF) synthesis. Thus, this work was concentrated on the maximum production of Hericium erinaceus mycelia. In order to maximize cell growth, the cultivation was performed with varying the agitation rate and applying the additional medium. As the results, the mycelium concentration was 24.2g/L.

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Pigmentation of egg yolks with astaxanthin from the yeast phaffia rhodozyma (이스트 phaffia rhodozyma 유래 astaxanthin의 난황착색에 관한 연구)

  • Kim, Ki-ha;An, Gil-hwan;Cho, Myung-haing;Lee, Sang-ho;Choi, Chi-man;Cho, Han-dug;Lee, Chang-hee;Moh, In-pill
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.463-470
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    • 1996
  • The red yeast Phaffia rhodozyma, which contains astaxanthin(3, 3'-dihydroxy-$\beta$, $\beta$-carotene-4, 4'-dione) as its primary carotenoid, was tested as a dietary pigment source for egg yolks of laying hens. When the yeast was fed to laying hens at several concentrations, the intensity of redness in egg yolks was dependent on the yeast concentration in the feed and the deposition period. Addition of P rhodozyma in feed did not cause any visible adverse effect on laying hens.

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A study on the resistance of saccharomyces cerevisiae to copper sulfate (유산동에 대한 Saccharomyce cerevisiae의 저항성에 관한 연구)

  • 이민재;이진기
    • YAKHAK HOEJI
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    • v.3 no.1
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    • pp.15-20
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    • 1957
  • Resume 1. The toxic effect of $CuSO_4$ on the growth of yeast began in the 0.2mM and colony formation was completely inhibited in the 3mM $CuSO_4$ media. 2. The yeast strain which was trained sucessively from lower concentration media to higher one, could grow even in 10mM $CuSO_4$ media. 3. Rlb strain produced brown pigment in copper media. 4. Resistance of Rlb strain to $CuSO_4$ did not revert in non copper media. 5. The appearance of resistant strain was regarded as the result of "Mutation and Selection". 6. The alcohol fermentation ability of Rlb strain was lower than that of parent strain. 7. Rlb strain yielded some effective substance which induced the parent strain to resist against $CuSO_4$. 8. The dehydrogenase activity of yeast was inhibited by $CuSO_4$.

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Effect of Ailanthi Radicis Cortex Extracts on Melanogenesis

  • Cho, Young-Ho
    • Biomedical Science Letters
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    • v.13 no.4
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    • pp.313-317
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    • 2007
  • Melanogenesis refers to the biosynthesis of melanin pigment in melanocytes. Melanogenesis is controlled by the intra- and extracellular environments. In the present study, to develop a new whitening agent, it was investigated the antioxidant activity and the inhibitory effect of Ailanthi Radicis Cortex extract on tyrosinase activity and on melanogenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of ethylacetate fraction from Ailanthi Radicis Cortex was higher than that of arbutin. The ethylacetate fraction showed scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also in ethylacetate fraction ($40.0{\pm}5%$ at the concentration of $400{\mu}g/ml$). This study demonstrates that the Ailanthi Radicis Cortex extract might be used to be a potential agent for skin whitening.

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Analysis of the Effect of Mordants on the Degradation of Alizarin in Silk Dyed with Natural Madder Dye

  • Li, Longchun;Ahn, Cheunsoon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.43 no.2
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    • pp.228-242
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    • 2019
  • This research investigated the effect of mordants on the degradation of madder dye in silk when silk was treated by the H2O2/UV condition as a laboratory simulation of burial induced degradation. Alum, iron, and alum/iron composite mordanting methods were applied to silk before dyeing with madder dye. Dye extracted from silk was examined using HPLC-DAD-MS analysis. The abundance of the chromatogram peak at 8.88 min retention time was used as the concentration of alizarin pigment in silk. K/S values, CIE $L^{\ast}a^{\ast}b^{\ast}$ values; in addition, Munsell HVC values were obtained using a spectrocolorimeter. The findings indicated that alizarin degraded most severely in silk mordanted by alum/iron composite mordanting than alum mordanting or iron mordanting. Mordanting with alum alone provided a relatively lower dye fixation at the point of dyeing; however, it provided a better survival of alizarin after 12 hours of degradation treatment.