• Proceedings of the Korean Nutrition Society Conference
• /
• 2003.05a
• /
• pp.257.2-257.2
• /
• 2003

• Proceedings of the PSK Conference
• /
• 2002.04a
• /
• pp.170.2-170.2
• /
• 2002

• Journal of Yeungnam Medical Science
• /
• v.40 no.4
• /
• pp.461-465
• /
• 2023

• Journal of Ginseng Research
• /
• v.43 no.2
• /
• pp.209-217
• /
• 2019

Background: Ginseng is a traditional herbal medicine for human health. Ginseng contains a bioactive ligand named gintonin. The active ingredient of gintonin is lysophosphatidic acid C18:2 (LPA C18:2). We previously developed a method for gintonin-enriched fraction (GEF) preparation to mass-produce gintonin from ginseng. However, previous studies did not show the presence of other bioactive lipids besides LPAs. The aim of this study was to quantify the fatty acids, lysophospholipids (LPLs), and phospholipids (PLs) besides LPAs in GEF. Methods: We prepared GEF from white ginseng. We used gas chromatography-mass spectrometry for fatty acid analysis and liquid chromatography-tandem mass spectrometry for PL analysis, and quantified the fatty acids, LPLs, and PLs in GEF using respective standards. We examined the effect of GEF on insulin secretion in INS-1 cells. Results: GEF contains about 7.5% linoleic (C18:2), 2.8% palmitic (C16:0), and 1.5% oleic acids (C18:1). GEF contains about 0.2% LPA C18:2, 0.06% LPA C16:0, and 0.02% LPA C18:1. GEF contains 0.08% lysophosphatidylcholine, 0.03% lysophosphatidylethanolamine, and 0.13% lysophosphatidylinositols. GEF also contains about 1% phosphatidic acid (PA) 16:0-18:2, 0.5% PA 18:2-18:2, and 0.2% PA 16:0-18:1. GEFmediated insulin secretion was not blocked by LPA receptor antagonist. Conclusion: We determined four characteristics of GEF through lipid analysis and insulin secretion. First, GEF contains a large amount of linoleic acid (C18:2), PA 16:0-18:2, and LPA C18:2 compared with other lipids. Second, the main fatty acid component of LPLs and PLs is linoleic acid (C18:2). Third, GEF stimulates insulin secretion not through LPA receptors. Finally, GEF contains bioactive lipids besides LPAs.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.21 no.3
• /
• pp.658-665
• /
• 2007

Phyllostachys pubescens (Maengiong-Juk), a kind of the bamboo, was reported to have many beneficial pharmacological actions. in this study, of using 70% ethanol extract of Phyllostachys pubescens we investigated its efficacy on angiotensin converting enzyme (ACE) and antioxidant enzyme activities. In addition, vasorelaxant effect was examined in rat aortic rings. The inhibitory effect of ACE activity by Phyllostachys pubescens extract (PPE) was dose-dependently increased by 61.42% at 10mg/ml. PPE relaxed the pre-contracted rat aortic rings with 10$^{-6}$M phenylephrine, showing about 88% at 4.0mg/ml. Sprague Dawley (SD) rats were given different concentrations of PPE mixed in the drinking water for 10 weeks. PPE did not show any difference with control group in blood pressure, body weight (BW) and food intake. However, it revealed the highest total antioxidative effect at dose of 1.0 g/100 g BW in plasma by TEAC assay. Thiobarbituric acid reactive substance (TBARS) and protein carbonyl levels which are markers of tissue peroxidation, were significantly lowed at the same dosage. Furthermore, hepatic antioxidant enzymes such as total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and catalase activities were also significantly increased by PPE (1.0 g/100 g BW). In conclusion, we suggest that PPE might have antihypertensive effect through increasing antioxidant activities.

• Proceedings of the KOSOMBE Conference
• /
• v.1997 no.05
• /
• pp.453-456
• /
• 1997

In this paper, we present the developement of Biofeedback system. Biofeedback is a method for learned control of physiological response of the body. It can be used to control certain biological response that cause health problems. Over the past 2 decades, biofeedback has received much attention from medicine as a cure-all for a variety of disease. It has been heralded as a technique that can provide normalization of disturbed physiology without the need for drugs or surgery and their attendant negative side effects. Biofeedback system has been implemented by using PIC microprocessor. We have displayed 5-channel value on PC and managed patient information by using Delphi.

• Journal of the Ergonomics Society of Korea
• /
• v.32 no.4
• /
• pp.303-308
• /
• 2013

Objective: The aim of this study is to understand health effect of hand-arm transmitted vibration. Background: Electronic vibration tools have been used in many industries. These tools evoke hand-arm transmitted vibration that cause hand-arm vibration syndrome. Method: Various papers on the hand-arm transmitted vibration and its health effects have been reviewed to know what health effect caused. Results: Hand-arm transmitted vibration causes circulatory, neural, musculoskeletal and other disturbance. Conclusion: The previous studies focused on the epidemiology and physiology for hand-arm transmitted vibration. So, now we should focus on a prevention and guideline of hand-arm transmitted vibration. Application: These results can be used to know health effect of hand-arm transmitted vibration.

• The Korean Journal of Physiology and Pharmacology
• /
• v.1 no.5
• /
• pp.477-484
• /
• 1997

Intracellular recordings of oscillatory firing (bursting activity) were obtained from Purkinje cells (PCs) in rat cerebellar slices. Apamin inhibited post-burst hyperpolarizations (PBHs) progressively and finally terminated oscillatory firing activity of PCs. Apamin did not affect the amplitude or duration of the after-hyperpolarization (AHP) between spikes within the burst. In the voltage clamp mode, apamin shifted the whole-cell, quasi-steady state I/V relationship in an inward direction and abolished the zero slope resistance (ZSR) region by blocking outward current. Nickel ($Ni^{2+}$) terminated oscillatory activity and also abolished the ZSR region. However, $Ni^{2+}$ did not have progressive blocking action on the post-burst hyperpolarization before it blocked oscillatory activity. $Ni^{2+}$ blocked an inward current at potentials positive to approximately -65 mV, which was responsible for the ZSR region and outward current at more negative potentials. These data indicated that oscillatory activity of PCs is sustained by a balance between a slow $Ni^{2+}$-sensitive inward current and an apamin-sensitive outward current in the region of ZSR of the whole-cell I/V curve.

• Women's Health Nursing
• /
• v.9 no.4
• /
• pp.489-498
• /
• 2003

Purpose: The purpose of this study was to measure the knowledge. Attitude and the needs of sex education for college students in order to provide basic sex education information. Method: For this study, the questionnaires were given to 320 students in the H, D colleage of Daegu and collected during the period from August 25 to October 25, 2003. These results were analyzed statistically by means of, frequency, percentage, t-test, chi-squire test. Result: Female students have a high score of sexual knowledge about genital physiology, sex physiology, V. D. and contraceptive. Both gender in sexual knowledge, male students correctly responded 86.4% form ejaculating sperm from the penis. Females on the other hand correctly responded 96% in the highest score to menstruation blood through out the vagina. Both gender male and female is wished to be co-education in sex education in college at a freshmen level. Sex education should be under by special lecture when education is in progress. Males student want to learn more about V. D. Females in the other hand want to learn a lot about contraceptive. Conclusion: In conclusion college sex education should be basic. knowledgeable and for the future of there on going marriage. It should be responsible in sex education, it should run under regular curriculum in college.

• Journal of Embryo Transfer
• /
• v.28 no.1
• /
• pp.57-61
• /
• 2013

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.