Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Microtubule-associated Protein 1B Interacts with Glyceraldehydes 3-Phosphate Dehydrogenase in Bovine Follicles

한우 난포에서 발현하는 MAP1B와 GAPDH의 상호 결합

  • Choe, Changyong (National Institute of Animal Science, RDA) ;
  • Han, Jaehee (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Kang, Dawon (Department of Physiology, Institute of Health Sciences, Gyeongsang National University School of Medicine)
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 한재희 (경상대학교 의학전문대학원 생리학교실.건강과학연구원) ;
  • 강다원 (경상대학교 의학전문대학원 생리학교실.건강과학연구원)
  • Received : 2013.02.25
  • Accepted : 2013.03.06
  • Published : 2013.03.31
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Abstract

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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