Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Analysis of Uterine Bacteria to Increase Reproductive Efficiency in Hanwoo(Korean Native Cattle)

한우의 번식 효율 향상을 위한 자궁 내 세균 분석

  • Park, Joung-Jun (Animal Reproduction & Biotechnology Center (ARBC)) ;
  • Yoo, Han-Jun (Animal Reproduction & Biotechnology Center (ARBC)) ;
  • Cho, Young-Jae (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Choi, Hye-Won (Animal Reproduction & Biotechnology Center (ARBC)) ;
  • Yoon, Pil-Sang (Animal Reproduction & Biotechnology Center (ARBC)) ;
  • Lee, Seon-Goo (College of Oriental Medicine, Sangji University) ;
  • Jung, Bae-Dong (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
  • Received : 2013.02.28
  • Accepted : 2013.03.08
  • Published : 2013.03.31
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Abstract

The objective of this study was to evaluate several types of uterine bacteria in Hanwoo. uterine bacteria from randomly selected 5 uterus was collected by flushing methods into a sterilized 1.5 ml centrifuge tube and was inoculated onto MacConkey agar and blood agar, respectively. After being incubated for 5% $CO_2$, aerobic or anaerobic condition at $37^{\circ}C$ during 48h, bacterial colonies were selected and re-inoculated onto blood agar plates. Re-cultured colonies were identified by Gram staining and finally identified using Vitek system. The identified bacteria were Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus vitulinus, Staphylococcus warneri of Gram (+) and Rhizobium radiobacter, Sphingomonas paucimobilis of Gram (-) bacteria. Although, pathogenicity of identified bacteria was unclear, the bacteria can have an effect on the uterine microenvironment. Therefore, repetitive research will be required to determine the effects of bacteria in cattle exposed to a various environment.

Keywords

Acknowledgement

Supported by : 농림수산식품부

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