Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

The Motility of Bovine Sperm and Fertilization Rate of Oocytes during in-vitro Fertilization following Oviduct Epithelial Cell Co-culture

소 정자의 운동성 향상에 따른 수정율 증진 연구

  • Lee, Hae-Lee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research) ;
  • Park, Jae-Hee (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research) ;
  • Kim, Yong-Su (Livestock Experiment Station, Jeollabuk-do Institute of Livestock and Veterinary Research) ;
  • Kim, Jong Gug (Department of Animal Science and Institute of Rare Earth for Biological Application, College of Agriculture and Life Science, Chonbuk National University)
  • 이해이 (전라북도축산위생연구소 축산시험장) ;
  • 박재희 (전라북도축산위생연구소 축산시험장) ;
  • 김용수 (전라북도축산위생연구소 축산시험장) ;
  • 김종국 (전북대학교 동물자원과학과/희토생물응용연구소)
  • Received : 2013.02.26
  • Accepted : 2013.03.07
  • Published : 2013.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture. The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours. After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.

Keywords

Acknowledgement

Supported by : Rural Development Administration

References

  1. Beckey M and Suarez SS. 2004. Different signaling pathways in bovine sperm regulate capacitation and hyperactivation. Biol. Reprod. 70:1626-1633. https://doi.org/10.1095/biolreprod.103.026476
  2. Brackett BG and Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12:260-274. https://doi.org/10.1095/biolreprod12.2.260
  3. Chian RC and Sirard MA. 1995. Fertilizing ability of spermatozoa cocultured with oviduct epithelial cells. Biol. Reprod. 52:156-162. https://doi.org/10.1095/biolreprod52.1.156
  4. Coy P, Garcia-Vazquez FA, Visconti PE and Aviles M. 2012. Roles of the oviduct in mammalian fertilization. Reproduction 144:649-660. https://doi.org/10.1530/REP-12-0279
  5. Demott RP and Suarez SS. 1992. Hyperactivated sperm progress in the mouse oviduct. Biol. Reprod. 46:779-785. https://doi.org/10.1095/biolreprod46.5.779
  6. DeMott RP, Lefebvre R and Suarez S. 1995. Carbohydrates mediate the adherence of hamster sperm to oviductual epithelium. Biol. Reprod. 52:1395-1403. https://doi.org/10.1095/biolreprod52.6.1395
  7. Dobrinski I, Ignotz GG, Thomas PG and Ball BA. 1996. Role of carbohydrates in the attachment of equine spermatozoa to uterine tubal (oviductal) epithelial cells in vitro. Am. J. Vet. Res. 57:1635-1639.
  8. Ekhlasi-Hundrieser M, Gohr K, Wagner A, Tsolova M, Petrunkina A and Topfer-Petersen E. 2005. Spermadhesin AQN1 is a candidate receptor molecule involved in the formation of the oviductal sperm reservoir in the pig. Biol. Reprod. 73:536-545. https://doi.org/10.1095/biolreprod.105.040824
  9. Ellington JE, Padilla AW, Vredenburgh WL, Dougherty EP and Foote RH. 1991. Behavior of bull spermatozoa in bovine uterine tube epithelial cell co-culture : An in vitro model for the studying the cell interaction of reproduction. Theriogenology 35:977-989. https://doi.org/10.1016/0093-691X(91)90308-Z
  10. Gualtieri R and Talevi R. 2003. Selection of highly fertilization competent bovine spermatozoa through adhesion to the Fallopian tube epithelium in vitro. Reproduction 125 :251-258. https://doi.org/10.1530/rep.0.1250251
  11. Gwathmey TM, Ignotz GG and Suarez SS. 2003. PDC-109 (BSP-A1/A2) promotes bull sperm binding to oviductal epithelium in vitro and may be involved in forming the oviductal sperm reservoir. Biol. Reprod. 69:809-815. https://doi.org/10.1095/biolreprod.102.010827
  12. Hwang WS, Roh SH and Lee BC. 1998. Fertilizing ability of bovine spermatozoa following oviduct epithelial cell coculture in vitro. J. Embryo Transf. 13: 227-233.
  13. Ignotz G, Lo M, Perez C, Gwathmey T and Suarez S. 2001. Characterization of a fucose-binding protein from bull sperm and seminal plasma that may be responsible for formation of the oviductal sperm reservoir. Biol. Reprod. 64:1806- 1811. https://doi.org/10.1095/biolreprod64.6.1806
  14. Iqbal N and Hunter AG. 1995. Effect of various capacitation systems on bovine sperm motion characteristics, acrosome integrity, and induction of hyperactivation. J. Dairy Sci. 78:91-102. https://doi.org/10.3168/jds.S0022-0302(95)76620-6
  15. Lefebvre R and Suarez SS. 1996. Effect of capacitation on bull sperm binding to homologous oviductual epithelium. Biol. Reprod. 54:575-582. https://doi.org/10.1095/biolreprod54.3.575
  16. Pacey AA, Davies N, Warren MA, Barratt CL and Cooke ID. 1995. Hyperactivation may assist human spermatozoa to detach from intimate association with the endosalpinx. Hum. Reprod. 10:2603-2609. https://doi.org/10.1093/oxfordjournals.humrep.a135754
  17. Parrish JJ, Susko-Parrish JL, Uguz C and First NL. 1994. Differences in the role of cycle adenosine 3',5'-monophosphate during capacitation of bovine sperm by heparin or oviduct fluid. Biol. Reprod. 51:1009-1108.
  18. Pollard JW, Plante C, King WA, Hansen PJ, Betteridge KJ and Suarez SS. 1991. Fertilizing capacity of bovine sperm may be maintained by binding to oviductual epithelial cells. Biol. Reprod. 44:102-107. https://doi.org/10.1095/biolreprod44.1.102
  19. Roh SH, Lee BC and Hwang WS. 1999. The ability of in vitro cultured bovine oviduct epithelial cells in binding and maintaining motility of bull sperm. Korean J. Vet. Res. 39: 220-225.
  20. Schneider CS, Ellington JE and Wright RW. 1999. Relationship between bull field fertility and in vitro embryo production using sperm preparation methods with and without somatic cell co-culture. Theriogenology 51:1085-1098. https://doi.org/10.1016/S0093-691X(99)80013-2
  21. Smith TT and Yanagimachi R. 1991. Attachment and release of spermatozoa from the caudal isthmus of the hamster oviduct. J. Reprod. Fertil. 91: 567-573. https://doi.org/10.1530/jrf.0.0910567
  22. Sostaric E, Dieleman SJ, van de Lest CH, Colenbrander B, Vos PL, Garcia-Gil N and Gadella BM. 2008. Sperm binding properties and secretory activity of the bovine oviduct immediately before and after ovulation. Mol. Reprod. Dev. 75:60-74. https://doi.org/10.1002/mrd.20766
  23. Stauss CR, Votta TJ and Suarez SS. 1995. Sperm motility hyperactivation facilitates penetration of the hamster zona pellucida. Biol. Reprod. 53:1280-1285. https://doi.org/10.1095/biolreprod53.6.1280
  24. Suarez S. 1987. Sperm transport and motility in the mouse oviduct : observation in situ. Biol Reprod. 36:203-210. https://doi.org/10.1095/biolreprod36.1.203
  25. Suarez S. 2002. Formation of a resevior of sperm in the oviduct. Reprod. Domest. Anim. 37:140-143.
  26. Suzuki H and Foote RH. 1995. Bovine oviduct epithelial cells(BOEC) and oviducts: I. For embryo culture.II. Using SEM for studying interactions with spermatozoa. Microsc. Res. Tech. 31:519-530. https://doi.org/10.1002/jemt.1070310608