• IMMUNE NETWORK
• /
• v.4 no.1
• /
• pp.7-15
• /
• 2004

Background: The heat shock proteins (HSPs) play an important role in cellular protection mechanisms against physical or chemical stresses. In this study scFv antibodies specific for human HSP70.1 were isolated from a semi-synthetic human scFv library with the ultimate goal of developing anti-HSP70.1 intracellular antibody (intrabody) that may offer an attractive alternative to gene targeting to study the function of the protein in cells. Methods: A semi-synthetic human scFv display library ($5{\times}10^{8}$ size) was constructed using pCANTAB-5E vector and the selection of the library against bacterially expressed recombinant human HSP70.1 was attempted by panning. Results: Three positive clones specific for recombinant HSP70.1 were identified. All three clones used $V_{H}$ subgroup III. On the other hand, $V_{L}$ of two clones belonged to the kappa light chain subgroup I, but the other utilized $V_{k}$ subgroup IV Interestingly, these scFv molecules specifically reacted to the recombinant HSP70.1, yet failed to recognize native HSP70 induced in U937 human monocytic cells by heat treatment. Conclusion: Our results indicated that affinity selection of an scFv phage display library using recombinant antigens produced in E. coli might not guarantee the isolation of scFv antibody molecules specific for a native form of the antigen. Therefore, the source of target antigens needs to be chosen carefully in order to isolate biofunctional antibody molecules.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.46 no.3
• /
• pp.125-137
• /
• 2009

Acoustic emission-based techniques are being used for the nondestructive inspection of mechanical systems used in service. For reliable fault monitoring related to the crack growth, it is important to identify the dynamical characteristics as well as transient crack-related signals. Widely used methods which are based on physical phenomena of the three damage stages for detecting the crack growth have a problem that crack-related acoustic emission activities overlap in time, therefore it is insufficient to estimate the exact crack growth time. The proposed pattern recognition method uses the dynamical characteristics of acoustic emission as inputs for minimizing false alarms and miss alarms and performs the temporal clustering to estimate the crack growth time accurately. Experimental results show that the proposed method is effective for practical use because of its robustness to changes of acoustic emission caused by changes of pressure levels.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.4C
• /
• pp.230-238
• /
• 2005

An orthogonal frequency division multiplexing (OFDM) system consists of a number of independently modulated subcarriers and, thus, a high peak-to-average power ratio (PAR) can occur when the subcarriers are added coherently. The high PAR brings such disadvantages as an increased complexity of the analog-to-digital (ADC) and digital-to-analog (DAC) converters and a reduced efficiency of the radio frequency (RF) power amplifier. In this paper, we propose a novel PAR reduction scheme called selective mapping of partial tones (SMOPT). The SMOPT scheme has a reduced complexity, lower sensitivity to peak reduction tones (PRT) positions, and a shorter processing time as compared with the conventional tone reservation (TR) scheme. The performance of the SMOPT scheme is analyzed based on the IEEE 802.1la wireless local area network(WLAM) physical layer model. Numerical results show that the SMOPT scheme outperforms the TR scheme under various scenarios.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.34 no.12A
• /
• pp.982-990
• /
• 2009

The IEEE 802.15.4 wireless sensor network is composed of the unique sensor devices to monitor and collect physical or environmental conditions. The interests in a positioning technology, which is one of the environment monitoring technologies, are gradually increased according to the development of the sensor technology and IT infrastructure. Generally, it is difficult for the positioning system using RSSI (Received Signal Strength Indication) based implementation to get accurate position because of obstacles, RF wave's delay and multipath. Therefore, in this paper, we investigate the improved positioning technologies for RSSI-based positioning system. This paper also proposes the enhanced scheme to improve the accuracy of positioning system by applying the Gaussian Filter algorithm, which is widely used for enhancing the performance of image processing system. For the implementation of proposed scheme, we firstly make a look-up tables, which represent the distance between target node and master node and corresponding RSSI value of each target node which are recorded as an average value after investigating the characteristics of attenuation of transmitted signal By applying the pre-determined look-up tables and Gaussian Filtering in the proposed scheme, we analyzed the positioning performance and compared with other conventional RSSI-based positioning algorithms.

• Journal of KIISE:Information Networking
• /
• v.33 no.3
• /
• pp.201-217
• /
• 2006

In this paper, we collected the physical traces from high speed Internet backbone traffic and analyze the various characteristics of the underlying packet traces. Particularly, our work is focused on analyzing the characteristics of an anomalous traffic. It is found that in our data, the anomalous traffic is caused by UDP session traffic and we determined that it was one of the Denial of Service attacks. In this work, we adopted the unsupervised machine learning algorithm to classify the network flows. We apply the k-means clustering algorithm to train the learner. Via the Cramer-Yon-Misses test, we confirmed that the proposed classification method which is able to detect anomalous traffic within 1 second can accurately predict the class of a flow and can be effectively used in determining the anomalous flows.

• Journal of KIISE:Computing Practices and Letters
• /
• v.14 no.1
• /
• pp.13-21
• /
• 2008

In general, data communication among sensor nodes requires more energy than internal processing or sensing activities. In this paper, we propose a noble technique to reduce the number of packet transmissions necessary for sending/receiving queries/results among neighboring nodes with the help of context-aware routing tables. The important information maintained in the context-aware routing table is which physical properties can be measured by descendent nodes reachable from the current node. Based on the information, the node is able to eliminate unnecessary packet transmission by filtering out the child nodes for query dissemination or result relaying. The simulation results show that up to 80% of performance gains can be achieved with our technique.

• The Bulletin of The Korean Astronomical Society
• /
• v.40 no.1
• /
• pp.61.2-61.2
• /
• 2015

15P/Finlay is one of the Jupiter-Family Comets that has long been known since the late 19 century. The comet maintains the perihelion around 1.0 AU over a century, without showing any prominent activities (i.e. fragmentation or eruption) since the discovery. According to reports in unpublished observations, the comet exhibited an outburst in the middle of 2014 December. We conducted a imaging observation of 15P/Finlay just after the report, from 2014 December 23 to 2015 February 18 using three telescopes (the Okayama Astrophysical Observatory 50-cm telescope, the Ishigakijima Astronomical Observatory 105-cm telescope, and the Nishi-Harima Astronomical Observatory 2-m telescope), which constitute a portion of the OISTER (an inter-university observation network in the optical and infrared wavelengths). As a result of the frequent observations, we witnesses the second outburst around UT 2015 January 16. Such cometary outbursts draw the attention to researchers on ground that they could offer insight into the internal structure of comets, following a historical outburst occurred at 17P/Holmes on 2007 October 23. Although cometary outbursts have been often reported mostly in unpublished observations or unreviewed reports, it should be emphasized that there are not a sufficient number of astrophysical research which characterizes the physical properties by observing the aftermaths. This presentation provides a new observational result of 15P/Finlay outburst. Based on the morphological development of the dust cloud as well as the near-nuclear magnitude, we will derive the kinetic energy of the outburst. Finally we plan to compare the results of 15P/Finlay with those of analogical events at 17P/Holmes and P/2010 V1 (Ikeya-Murakami).

• Journal of the Korean Electrochemical Society
• /
• v.14 no.4
• /
• pp.239-244
• /
• 2011

In this study, $Fe_3O_4$/graphene and CuO/graphene composites were synthesized by the polyol reduction method using ethylene glycol, and their performances as the anodes of lithium ion batteries were evaluated. The physical characteristics of the synthesized composites were analyzed by SEM, XRD, and TGA. In addition, their electrochemical properties were examined by the electrochemical analysis techniques such as charge/discharge performance, cyclic voltammetry, and AC impedance spectroscopy. The cells composed of $Fe_3O_4$/graphene and CuO/graphene composites showed better performance than the graphene electrode, due to the dispersion of nanosized $Fe_3O_4$ or CuO on the surface of graphene and the formation of good electrical network in the electrode. Their composites kept the reversible capacity more than 600 mAh/g even after the charging/discharging of 30 cycles.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.24 no.12
• /
• pp.955-961
• /
• 2011

We investigated the physical properties of stoichiometric and non-stoichiometric oxide doped complex perovskite, $Ba(Zn_{1/3}Ta_{2/3})O_3$ ceramics and their impacts on the microwave dielectric performances using various characterization techniques such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and network analyzer. According to the measurement of lattice constant changes, anomalous lattice volume contraction of $ZrO_2$ doped $Ba(Zn_{1/3}Ta_{2/3})O_3$ sample only showed the dielectric quality factor enhancements, which was due to the lattice volume contraction as well as the 1:2 B-site cation ordering. In addition, NiO doping was useful to the stabilization of temperature coefficient of resonance frequency.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.15 no.11
• /
• pp.2480-2487
• /
• 2011

As sensor technology grows up in fields such as environmental hazards detecting system, ubiquitous sensor network, intelligent robot, the sensing and detecting system for sensor is increasing. The sensor data is measured by change of chemical and physical status. Because of decrepit sensor or various sensing environment, it is problem that sensor data is inaccurate result. So the reliability of sensor data is essential. In this paper, we proposes a reliable sensor signal processing platform for various sensor. To improve reliability, we use same sensors in multiple array structure. As sensor data is corrected by spatial and temporal relation signal processing algorithm for measured sensor data, reliability of sensor data can be improved. The exclusive protocol between platform components is designed in order to verify sensor data and sensor state in various environment.