• The Korean Journal of Pesticide Science
• /
• 제19권1호
• /
• pp.54-63
• /
• 2015

Streptomyces spp. isolated from turfgrass rhizosphere and tested for their response to large-patch control fungicides. The tested fungicides were actually used in golf course or turfgrass cultivation to prevent large-patch disease. Tolerance to 3 triazole group of the strains was the highest to the PR fungicide, and following the SR fungicide, whereas the isolated strains were no tolerance to HR fungicide. Tolerances to three kind of Strobilurin group were similar for the all of the tested Streptomyces spp.. Growth and sporulation of the all strain was normal in CB and AP fungicide treatments. However no spore formulated in double concentration. Strains, tolerance to acetanilide fungicides, appeared that KT fungicide tolerance was higher than MK fungicide. The selected strains showed strong tolerance against AT fungicide but have no tolerance to ATR fungicides. In conclusion, the bacterial strains showed tolerance against 1 carbamate, 1 organophosphate and 1 cyanopyrrole group, while have no tolerance against two mixture formulations (1 Quinone + Strobilurin and 1 Imidazole + Triazole).

• The Korean Journal of Pesticide Science
• /
• 제19권3호
• /
• pp.241-247
• /
• 2015

This study has been conducted to verify the applicability of FT-NIR (Fourier Transform Near Infrared) to prove coidentity between market agrochemical products and registered prescriptions. The spectrum correlations were investigated on 83 registered samples and there market products, 23 products which had same formulation and active ingredients and 68 products which had different active ingredients. In 83 samples/products, the primary differentiated spectrum correlation values were 95.86~100%, which mean that all samples qualified over 95% threshold. In 23 products which had same active ingredients and formulations, correlation values were 29.09~99.83%. 3 products over 99.0% were proved to have same active/inert ingredients and formulations although they're from different manufacturers. The rest products except the 3 items were under 95%. In 68 products had different active ingredients, correlation values varied from 2.00% to 93.70%. Higher correlation is supposed to come from similarity of inert ingredients despite different active ingredients. They can be decided to unqualify under 95% threshold. So applicability of FT-NIR has been verified on qualitative distinction of coidentity between registered market agrochemicals.

• The Korean Journal of Pesticide Science
• /
• 제16권4호
• /
• pp.343-349
• /
• 2012

Exposure and risk assessments were conducted to evaluate the relative safety of mixing/loading work of indoxacarb between wettable powder (WP) and water dispersible granule (WG). Hand exposure was monitored using cotton gloves while inhalation exposure was measured using personal air monitor. Method validation for the exposure monitoring was established successfully through several experiments. Limit of determination and limit of quantitation were 0.25 and 1 ng, respectively. $R^2$ of calibration curve linearity was more than 0.9999 and reproducibility was 0.7-6. Recovery of indoxacarb from gloves, solid sorbent and glass fiber filter at three different levels was 81.5-108.8%. Trapping efficiency and breakthrough tests gave 981.5-108.8% of recovery. During mixing/loading procedure, hand exposure amount (75 percentile of 30 repetitions) for indoxacarb WP was 6 folds (459.8 mg/kg a.i) than that of WG (81.4 mg/kg a.i). This result indicates that WG has less drift than WP thanks to its granular type of formulation. Inhalation amount was $10^{-8}-10^{-7}%$ of spray mixture prepared and $10^{-4}-10^{-3}%$ of hand exposure. In inhalation case, no significant differences were observed between two formulations. Margin of safety was calculated for risk assessment using male Korean average body weight and acceptable operator exposure level as the important exposure factors. Mixing/loading procedures for both of the formulations were considered to be of least risk because calculated MOS values were more than 1.

• The Korean Journal of Pesticide Science
• /
• 제15권1호
• /
• pp.55-60
• /
• 2011

To work out quality control methods of environmental-friendly organic materials (EFOMs), the reason and basis for EFOM-selection and farmer's favorite formulation type of EFOMs, etc were investigated on farmers who had been practicing environmental-friendly agriculture. EFOMs used were soil amendments, control agents of plant diseases and insect pests, plant growth promotion formulations, in turns. In EFOMs application time, 22.7% of farmers sprayed EFOMs without delay after they were bought, in other hand, 77.3% of farmers used EFOMs which had been bought and stored for some period. Microbial density on seventeen environmental-friendly microbial formulates (EFMFs) including microbial pesticides, a microbial fertilizer, and environmental-friendly organic materials was investigated at different storing temperature and shelf life. When the microbial density of EFMFs was investigated without delay after they were bought, all used microbial pesticides and a microbial fertilizer was confirmed to be optimal for the certified density but two of environmental-friendly organic materials was confirmed not to be optimal. When microbial density of 17 EFMFs were investigated after storing them for six months at $4^{\circ}C$, only one of 9 microbial pesticides was confirmed not to be optimal, the other hand four of seven environmental-friendly organic materials not to be optimal, which each of their microbial density was less than the certified density. Population dynamics of microbial agents was much more influenced in fluctuated temperature (room temperature) than in static temperature condition ($5^{\circ}C$ and $25^{\circ}C$). Shelf life of microbial agents according to microbial formulation type were high in granule type, liquid wettable type and liquid type in turns.

• Journal of Applied Biological Chemistry
• /
• 제61권2호
• /
• pp.151-155
• /
• 2018

In this study, cinnamon essential oil (CEO) was formulated as emulsifiable concentrate (EC) and a granule. For the evaluation of their negative effects on the ecosystem, acute toxicities against Cyprinus carpio was determined in a static condition. The formulations were made using CEOs extracted by 3 different methods (steam distillation (SD), solvent extraction and supercritical fluid extraction (SFE)) and were tested to obtain $LC_{50}$ values. Among the ECs, EC including CEO extracted by SFE showed highest acute toxicity against C. carpio. Among the granules, a granule including CEO extracted by SD showed highest acute toxicity against C. carpio. Nevertheless, $LC_{50}$ of EC and a granule formulation with CEOs was higher than toxicity level III of pesticide standardized by Korea rural development administration. These results were similar to those using zebrafishes. Chronic toxicities were not found for 45 days in zebrafishes until $500{\mu}gL^{-1}$ level of EC formulation including CEO obtained by the SD. Based on these results, EC formulation of CEOs may be considered to be used as environmental-friendly natural insecticides in accordance with the standards.

• Korean Journal of Weed Science
• /
• 제18권4호
• /
• pp.304-313
• /
• 1998

Foliar retention of pyribenzoxim (1% EC) was measured using the fluorescent dye rhodamine B, and related to efficacy and phytotoxicity to barnyardgarss (Echinochloa crusgalli) and rice (Oryza sativa cv. Chucheong), respectively. Effects of nozzle types (8002E flat-fan and disk-type), addition of adjuvant, variation of herbicide concentration or spray volume were compared. In barnyardgrass, foliar retention of pyribenzoxim at a recommended condition (application rate : 30g ai/ha, spray volume : 1000 L/ha) was 2.3 to 2.7 or 1.4 to $1.5{\mu}g$ ai/g fresh foliage when sprayed using the disk-type nozzle with or without adjuvant, respectively, and 0.6 to 0.7 or 0.3 to $0.5{\mu}g$ ai/g fresh foliage when sprayed using the flat-fan nozzle with or without adjuvant, respectively. The slope of increase in foliar retention was 1.0 to 1.8 when application rates increased from 10 to 60g ai/ha at 1000 L/ha, while that was 1.6 to 2.4 when spray volume increased from 330 to 2000 L/ha at $30{\mu}g$ ai/L concentration. Foliar retention of pyribenzoxim had a close relationship with herbicidal activity; regardless of spray conditions, retention to provide 90% control was about $0.8{\mu}g$ ai/g fresh foliage, and below this retention amount, efficacy decreased almost linearly. In rice, foliar retention at the recommended condition was 1.9 to 2.3 or 1.2 to $1.3{\mu}g$ ai/g fresh foliage when sprayed using the disk-type nozzle with or without adjuvant, respectively, and 0.6 to 0.9 or $0.3{\mu}g$ ai/g fresh foliage when sprayed using the flat-fan nozzle with or without adjuvant, respectively. The slope of increase in foliar retention was 1.0 to 2.8 when application rates increased from 30 to 120g ai/ha at 1000 L/ha, while that was 1.3 to 4.4 when spray volume increased from 1000 to 4000 L/ha at $30{\mu}g$ ai/L concentration. Despite the great difference in retention, rice phytotoxicity was not observed in any of these spray conditions, suggesting retention differences within 4-fold increase of application rate or spray volume do not affect rice safety. When pyribenzoxim 1EC was sprayed in tank-mix with several other commercial pesticide formulations, its retention to rice foliage tended to increase by 30 to 50%.