Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Ecotoxicities of emulsifiable concentrate and granules of cinnamon (Cinnamomum zeylanicum) essential oil against Cyprinus carpio and Danio rerio

계피(Cinnamomum zeylanicum) 정유의 유제 및 입제의 잉어(Cyprinus carpio) 및 제브라피쉬에 대한 생태독성

  • Nam, Tae-Hoon (School of Applied Biosciences, Kyungpook National University) ;
  • Jeon, Hwang-Ju (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Kyeongnam (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Hyeong-Mi (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Yong-Chan (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Sung-Eun (School of Applied Biosciences, Kyungpook National University)
  • Received : 2018.04.06
  • Accepted : 2018.04.19
  • Published : 2018.06.30
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Abstract

In this study, cinnamon essential oil (CEO) was formulated as emulsifiable concentrate (EC) and a granule. For the evaluation of their negative effects on the ecosystem, acute toxicities against Cyprinus carpio was determined in a static condition. The formulations were made using CEOs extracted by 3 different methods (steam distillation (SD), solvent extraction and supercritical fluid extraction (SFE)) and were tested to obtain $LC_{50}$ values. Among the ECs, EC including CEO extracted by SFE showed highest acute toxicity against C. carpio. Among the granules, a granule including CEO extracted by SD showed highest acute toxicity against C. carpio. Nevertheless, $LC_{50}$ of EC and a granule formulation with CEOs was higher than toxicity level III of pesticide standardized by Korea rural development administration. These results were similar to those using zebrafishes. Chronic toxicities were not found for 45 days in zebrafishes until $500{\mu}gL^{-1}$ level of EC formulation including CEO obtained by the SD. Based on these results, EC formulation of CEOs may be considered to be used as environmental-friendly natural insecticides in accordance with the standards.

계피정유를 유제 및 입제로 제형화한 후 이들의 생태독성을 측정하였다. 잉어를 이용한 급성독성에 사용된 계피정유는 수증기증류법, 핵산을 이용한 용매추출법 및 초임계 추출법을 이용하여 얻었고 이들의 유제 및 입제의 생태독성학적 영향은 반수치사농도($LC_{50}$)를 구하여 평가하였다. 유제 중 초임계추출법으로 얻은 정유를 함유한 제형이 잉어에 대하여 가장 높은 독성을 나타내었다. 입제 중 수증기 증류법으로 추출한 계피정유를 함유한 입제가 잉어에 대하여 가장 높은 급성독성을 보였다. 이러한 결과는 3급 어독성에 해당되는 것으로 환경 중 사용에는 문제가 없다고 사료된다. 잉어를 이용하여 얻은 급성독성 결과와 같이 제브라피쉬를 사용하여 얻은 급성독성 결과 값들은 유사하였다. 수증기증류법으로 얻은 계피정유를 함유한 유제에 노출된 제브라피쉬를 이용한 만성독성은 45일 동안 $500mgL^{-1}$ 수준에서 독성이 관측되지 않았다. 따라서, 계피정유의 유제 및 입제의 급만성 어독성은 친환경제재로 사용하기에 적합한 것으로 사료된다.

Keywords

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