The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Investigation of Fungicide Response of Streptomyces spp. Isolated from Rhizosphere in Zoysiagrass

한국 잔디 근권에서 분리된 Streptomyces spp.의 살균제에 대한 반응조사

  • Lee, Jung Han (Korea Turfgrass Research Institute) ;
  • Min, Gyu Young (Daejung Golf Engineering Co. Ltd.) ;
  • Jeon, Chang Wook (Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Choi, Su Min (Southern Forest Resource Research Center, Korea Forestry Research Institute) ;
  • Shim, Gyu Yul (Korea Turfgrass Research Institute) ;
  • Kwak, Youn-Sig (Institute of Agriculture & Life Science, Gyeongsang National University)
  • 이정한 (한국잔디연구소) ;
  • 민규영 (대정골프엔지니어링) ;
  • 전창욱 (경상대학교농업생명과학연구원) ;
  • 최수민 (국립산림과학원 남부산림자원연구소) ;
  • 심규열 (한국잔디연구소) ;
  • 곽연식 (경상대학교농업생명과학연구원)
  • Received : 2015.02.03
  • Accepted : 2015.03.26
  • Published : 2015.03.31
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Abstract

Streptomyces spp. isolated from turfgrass rhizosphere and tested for their response to large-patch control fungicides. The tested fungicides were actually used in golf course or turfgrass cultivation to prevent large-patch disease. Tolerance to 3 triazole group of the strains was the highest to the PR fungicide, and following the SR fungicide, whereas the isolated strains were no tolerance to HR fungicide. Tolerances to three kind of Strobilurin group were similar for the all of the tested Streptomyces spp.. Growth and sporulation of the all strain was normal in CB and AP fungicide treatments. However no spore formulated in double concentration. Strains, tolerance to acetanilide fungicides, appeared that KT fungicide tolerance was higher than MK fungicide. The selected strains showed strong tolerance against AT fungicide but have no tolerance to ATR fungicides. In conclusion, the bacterial strains showed tolerance against 1 carbamate, 1 organophosphate and 1 cyanopyrrole group, while have no tolerance against two mixture formulations (1 Quinone + Strobilurin and 1 Imidazole + Triazole).

골프장이나 잔디 재배지에서 분리된 방선균을 대상으로 잔디병 방제 약제에 대한 약제 반응 조사한 결과 트리아졸계(Triazole) 약제 3종에 대한 반응은 PR 약제의 경우 시험 균주에 가장 영향을 미치지 않았고 다음으로 SR 약제로 나타났다. 반면에 HR 약제에 대하여 모든 균주는 생장하지 않는 것으로 나타났다. 스트로빌루린계(Strobilurin) 3종에 대한 반응은 선발된 균주 모두 유사하게 나타났다. CB와 AP 약제 경우 균주의 생장과 포자형성이 잘되었으나 CBA 약제 경우 2배 농도에서 선발된 균주 모두 포자형성이 되지 않았다. 아닐라이드계(Acetanilide) 2종에 대한 반응은 MK 약제보다 KT 약제 잘 생장하였다. 유기유황계(Organosulfur) 2종 대한 약제반응은 AT 약제에서 잘 생장하였으며 ATR 약제에서는 자라지 않는 것으로 나타났다. 카바메이트계(Carbamate) 1종, 유기인계(Organophosphate) 1종과 시아노피롤계(Cyanopyrrole) 1종에 대하여 시험 균주는 생장에 거의 영향을 받지 않는 것으로 나타났으나 퀴논계 + 스트로빌루린계(Quinone + Strobilurin) 혼합제 1종과 이미다졸계 + 트리아졸계(Imidazole + Triazole) 1종에 대하여는 전혀 생장하지 않는 것으로 나타났다.

Keywords

References

  1. Abdel-Moity, H., G. C. Papavizas and M. N. Shatia (1982) Induction of new isolates of Trichoderma harzianum tolerant to fungicides and their experimental use for control of white rot of onion. Phytopathology. 72:396-400. https://doi.org/10.1094/Phyto-72-396
  2. Altuntas, I., N. Delibas and R. Sutcu (2002) The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidant enzymes in rat erythrocytes: role of vitamins E and C. Hum. Exp. Toxicol. 21:681-685. https://doi.org/10.1191/0960327102ht304oa
  3. Anitha, A. and M. Rebeeth (2009) In vitro antifungal activity of Streptomyces griseus against phytopathogenic fungi of tomato field. Acad. J. Plant Sci. 2:119-123.
  4. Avenot, H. F. and T. J. Michailides (2010) Progress in understanding molecular mechanisms and evolution of resistance to succinate dehydrogenase inhibiting (SDHI) fungicides in phytopathogenic fungi. Crop Prot. 29:643-651. https://doi.org/10.1016/j.cropro.2010.02.019
  5. Chandler, D., A. S. Bailey, G. M. Tatchell, G. Davidson, J. Greaves and W. P. Grant (2011) The development, regulation and use of biopesticides for integrated pest management. Philos. Trans. R. Soc. Lond. B Biol. Sci. 366:1987-1998. https://doi.org/10.1098/rstb.2010.0390
  6. El-Tarabily, K. and St. J. Hardy (2000) Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol. 49:573-583. https://doi.org/10.1046/j.1365-3059.2000.00494.x
  7. Errakhi, R., F. Bouteau, A. Lebrihi and M. Barakate (2007) Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.). World J. Microbiol. Biotechnol. 23:1503-1509. https://doi.org/10.1007/s11274-007-9394-7
  8. Ezziyyani, M., M. E. Requena, C. Egea-Gilabert and M. E. Candela (2007) Biological control of Phytophthora root rot of pepper using Trichoderma harzianum and Streptomyces rochei in combination. J. Phytopathol. 155:342.349.
  9. Fernandez-Ortuno D, J. A.Tores, A. de Vicente and A. Perez- Garcia (2008) Mechanisms of resistance to QoI fungicides in phytopathogenic fungi. Int. Microbiol. 11:1-9.
  10. Gisi, U. and H. Sierotzki (2008) Fungicide modes of action and resistance in downy mildews. Eur. J. Plant Pathol. 122:157-167. https://doi.org/10.1007/s10658-008-9290-5
  11. Guan, Q., C. Han, D. Zuo, M. Zhai, Li, Z. Zhang, Q. et al. (2014) Synthesis and evaluation of benzimidazole carbamates bearing indole moieties for antiproliferative and antitubulin activities. Eur. J. Med. Chem. 24:306-315.
  12. Lechevalier, M. P. (1988) Actinomycetes in agriculture and forestry. In Actinomycetes in Biotechnology Goodfellow, M. Williams, S.T. Mordarski, M. Eds;Academic Press: London, pp. 327- 348.
  13. Maldonado, M. C., C. E. Orosco, M. A. Gordillo and A. R. Navarro (2010) In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum. Afr. J. Microbiol. Res. 4:2451-2556.
  14. Mishra, A., S. D. Sharma and S. I. Patel (2004) Cross tolerance of Trichoderma harzianum Rifai to fungicides. Indian Phytopathol. 38:207-211.
  15. Mohiddin, F. A. and M. R. Khan (2013) Tolerance of fungal and bacterial biocontrol agents to six pesticides commonly used in the control of soil borne plant pathogens. Afr. J. Agric. Res. 8:5331-5334.
  16. Papavizas, G. C., J. A. Lewis and T. H. Abdel Moity (1982) Evaluation of new biotypes of T. harzianum for tolerance to benomyl and enhanced biocontrol capabilities. Phytopathology. 72:126-132. https://doi.org/10.1094/Phyto-72-126
  17. Sabaratnam, S. and J. A. Traquair (2002) Formulation of a Streptomyces biocontrol agent for the suppression of Rhizoctonia damping-off in tomato transplants. Biol. Cont. 23:245-253. https://doi.org/10.1006/bcon.2001.1014
  18. Siegel, M. R. (1981) Sterol-inhibiting fungicides: effects on sterol biosynthesis and sites of action. Plant Dis. 65:986-989. https://doi.org/10.1094/PD-65-986
  19. Valois, D., K. Fayad, T. Barasubiye, M. Caron, C. Dery, R. Brzezinski and C. Beaulieu (1996) Glucanolytic actinomycetes antagonistic to Phytophthora fragariae var. rubi, the causal agent of Raspberry root rot. Appl. Environ. Microbiol. 5:1630-1635.
  20. Yoshimi, A., K. Kojima, Y. Takano and C. Tanaka (2005) Group III histidine kinase is a positive regulator of hog1- type mitogen-activated protein kinase in filamentous fungi. Eukaryot Cell. 4:1820-1828. https://doi.org/10.1128/EC.4.11.1820-1828.2005

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