• The Plant Pathology Journal
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• 제38권4호
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• pp.345-354
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• 2022

NADPH oxidase (Nox) complexes are known to play essential roles in differentiation and proliferation of many filamentous fungi. However, the functions of Noxs have not been elucidated in Colletotrichum species. Therefore, we set out to characterize the roles of Nox enzymes and their regulators in Colletotrichum scovillei, which causes serious anthracnose disease on pepper fruits in temperate and subtropical and temperate region. In this study, we generated targeted deletion mutants for CsNox1, CsNox2, CsNoxR, and CsNoxD via homologous recombination. All deletion mutants were normal in mycelial growth, conidiation, conidial germination, and appressorium formation, suggesting that CsNox1, CsNox2, CsNoxR, and CsNoxD are not involved in those developmental processes. Notably, conidia of 𝜟Csnox2 and 𝜟Csnoxr, other than 𝜟Csnox1 and 𝜟Csnoxd, failed to cause anthracnose on intact pepper fruits. However, they still caused normal disease on wounded pepper fruits, suggesting that Csnox2 and CsnoxR are essential for penetration-related morphogenesis in C. scovillei. Further observation proved that 𝜟Csnox2 and 𝜟Csnoxr were unable to form penetration peg, while they fully developed appressoria, revealing that defect of anthracnose development by 𝜟Csnox2 and 𝜟Csnoxr resulted from failure in penetration peg formation. Our results suggest that CsNox2 and CsNoxR are critical for appressorium-mediated penetration in C. scovillei-pepper fruit pathosystem, which provides insight into understanding roles of Nox genes in anthracnose disease development.

• 식물병연구
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• 제10권3호
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• pp.203-208
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• 2004

붉은 고추 탄저병균의 우점종인 Colletotrichum gloeosporioides는 C. acutatum에 비해 병원성이 강하였다. C. gloeosporioides는 G와 R계통이 존재하였으며, G계통이 R계통보다 더욱 강한 병원성을 보였다. 붉은 고추 품종간의 병원성 검정 결과, 금세기 품종이 가장 감수성이었고, 파페트 품종이 가장 약한 감수성이었다. 참깨, 홍화, 마, 그리고 딸기와 같은 다른 기주에서 분리되어진 C. gloeosporioides는 붉은 고추에 감염을 일으켰다.

• 농약과학회지
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• 제9권1호
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• pp.102-107
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• 2005

고추 탄저병과 역병균에 항균활성을 보이는 미생물을 산림과 비 경작지 토양으로부터 분리하였다. 대치배양, 배양액 및 열매를 이용한 간이 검정을 통하여 Streptomyces sp. 200401 균주가 최종 선발되었다. 선발 균주는 16S rDNA 염기서열과 지방산 분석을 통하여 Streptomyces griseofuscus으로 동정되었다.

• The Plant Pathology Journal
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• 제24권1호
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• pp.17-23
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• 2008

A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.

• 식물병연구
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• 제16권1호
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• pp.66-73
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• 2010

2008년 수원과 안동 시헝포장과 2009년 경북 봉화와 충남 태안 농가포장에서 역병, 담배나방, 탄저병의 병충해를 관리하는 고추 종합 예찰 방제 타당성 평가를 실시 하였다. 이병과율과 수확량이 무방제, 예찰방제, 관챙방제 등 세 처리 간에 비교평가 하였다. 2009년 봉화의 두 품종은 예찬방제 처리의 이병과율과 수확량이 각각 3.1-3.5%와 30-33 kg/40주 였 다. 이 는 관행 방제 의 0.3-0.8%와 35-36kg/40주와 비슷하면서도 무방제의 23-27%와 18-24 kg/40주보다 월등한 방제였다. 예찰방제 살포 횟수는 g회 였으나 농가에서 관행방제는 11-19회 였다. 또한 다른 세 포장에서의 탄저병 예찰방제의 결과에서도 탄저병을 방제하면서도 관행방제보다 세균제 사용량과 방제 비용을 줄일 수 있다는 결과를 보여주었다. 그런데, 무방제 탄저병 이병과율이 60-80%이상으로 극심했던 태안 농가의 경우 탄저병 예찰 방제의 보완이 필요하다. 왜냐하면 예찰방제의 방제가는 겨우 무방제와 관행방제의 중간 정도에 머물렀기 때문이다. 만일 재배 초기에 탄저병 발병이 심한 포장이라면, 아직 과실이 익기 전이라도 추가적인 예방 방제가 필요하다.

• Journal of Applied Biological Chemistry
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• 제48권3호
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• pp.151-152
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• 2005

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.13-16
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• 2008

Pepper fruit anthracnose caused by Colletotrichum species is an economically important disease that causes serious yield loss and quality deterioration in many Asian countries including South Korea and Taiwan. Recently, Capsicum baccatum PI594137 was found to exhibit broad-spectrum resistance to Colletotrichum acutatum. The inheritance of anthracnose resistance to C. acutatum was analyzed in an intraspecific population derived from a cross between C. baccatum Golden-aji and PI594137. Detached mature green fruits were inoculated using the microinjection method. The disease response was evaluated as the disease incidence and the overall lesion diameter at 7 days after inoculation(DAI). The segregation ratios of resistance and susceptibility to C. acutatum in the $F_2$ and $BC_s$ populations significantly fit a 3:1 Mendelian model. This result indicates that the resistance of PI594137 to C. acutatum is controlled by a single dominant gene.

• 식물병연구
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• 제16권1호
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• pp.106-108
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• 2010

노지피복 재배포장과 비닐터널유인 재배포장의 재배양식별 고추 탄저병과 역병의 발생정도를 조사하였다. 비닐터널유인재배포장의 초기 생육이 노지피복재배포장(관행)보다 월등히 우세하였다. 재배 양식별 고추 주요 병해인 탄저병과 역병의 발생정도를 조사한 결과 노지피복재배포장에 비해 터널유인 재배한 포장에서 역병발생은 60.3%, 탄저병발생은 26.9% 경감효과가 있었다. 재배양식별 건과 수확량을 조사한 결과 노지피복 재배포장대비 48.5%가 많은 것으로 조사되었다.

• The Plant Pathology Journal
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• 제38권6호
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• pp.616-628
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• 2022

Resistance to pyraclostrobin due to a single nucleotide polymorphism at 143rd amino acid position on the cytochrome b gene has been a major source of concern in red pepper field infected by anthracnose in Korea. Therefore, this study investigated the response of 24 isolates of C. acutatum and C. gloeosporioides isolated from anthracnose infected red pepper fruits using agar dilution method and other molecular techniques such as cytochrome b gene sequencing, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and allele-specific polymerase chain reaction (PCR). The result showed that four isolates were resistant to pyraclostrobin on agar dilution method and possessed GCT (alanine) codon at 143rd amino acid position, whereas the sensitive isolates possessed GGT (glycine). Furthermore, this study illustrated the difference in the cytochrome b gene structure of C. acutatum and C. gloeosporioides. The use of cDNA in this study suggested that the primer Cacytb-P2 can amplify the cytochrome b gene of both C. acutatum and C. gloeosporioides despite the presence of various introns in the cytochrome b gene structure of C. gloeosporioides. The use of allele-specific PCR and PCR-RFLP provided clear difference between the resistant and sensitive isolates. The application of molecular technique in the evaluation of the resistance status of anthracnose pathogen in red pepper provided rapid, reliable, and accurate results that can be helpful in the early adoption of fungicide-resistant management strategies for the strobilurins in the field.

• Mycobiology
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• 제50권6호
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• pp.467-474
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• 2022

Autophagy serves as a survival mechanism and plays important role in nutrient recycling under conditions of starvation, nutrient storage, ad differentiation of plant pathogenic fungi. However, autophagy-related genes have not been investigated in Colletotrichum scovillei, a causal agent of pepper fruit anthracnose disease. ATG8 is involved in autophagosome formation and is considered a marker of autophagy. Therefore, we generated an ATG8 deletion mutant, ΔCsatg8, via homologous recombination to determine the functional roles of CsATG8 in the development and virulence of C. scovillei. Compared with the wild-type, the deletion mutant ΔCsatg8 exhibited a severe reduction in conidiation. Conidia produced by ΔCsatg8 were defective in survival, conidial germination, and appressorium formation. Moreover, conidia of ΔCsatg8 showed reduced lipid amount and PTS1 selectivity. A virulence assay showed that anthracnose development on pepper fruits was reduced in ΔCsatg8. Taken together, our results suggest that CsATG8 plays various roles in conidium production and associated development, and virulence in C. scovillei.