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PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic-pituitary-gonadal axis in sows

  • Kim, Hwan-Deuk;Jo, Chan-Hee;Choe, Yong-Ho;Lee, Hyeon-Jeong;Jang, Min;Bae, Seul-Gi;Yun, Sung-Ho;Lee, Sung-Lim;Rho, Gyu-Jin;Kim, Seung-Joon;Lee, Won-Jae
    • Animal Bioscience
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    • v.35 no.12
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    • pp.1850-1859
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    • 2022
  • Objective: The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression. Endogenous reference genes (RGs) have been used to normalize qPCR data, although their expression may vary in different tissues and experimental conditions. Verification of the stability of RGs in selected samples is a prerequisite for reliable results. Therefore, we attempted to identify the most stable RGs in the hypothalamic-pituitary-gonadal (HPG) axis in sows. Methods: The cycle threshold values of nine commonly used RGs (18S, HPRT1, GAPDH, RPL4, PPIA, B2M, YWHAZ, ACTB, and SDHA) from HPG axis-related tissues in the domestic sows in the different stages of estrus cycle were analyzed using two RG-finding programs, geNorm and Normfinder, to rank the stability of the pool of RGs. In addition, the effect of the most and least stable RGs was examined by normalization of the target gene, gonadotropin-releasing hormone (GnRH), in the hypothalamus. Results: PPIA, HPRT1, and YWHAZ were the most stable RGs in the HPG axis-related tissues in sows regardless of the stages of estrus cycle. In contrast, traditional RGs, including 18S and ACTB, were found to be the least stable under these experimental conditions. In particular, in the normalization of GnRH expression in the hypothalamus against several stable RGs, PPIA, HPRT1, and YWHAZ, could generate significant (p<0.05) elevation of GnRH in the preovulatory phase compared to the luteal phase, but the traditional RGs with the least stability (18S and ACTB) did not show a significant difference between groups. Conclusion: These results indicate the importance of verifying RG stability prior to commencing research and may contribute to experimental design in the field of animal reproductive physiology as reference data.

Experiment and Analysis of Backscattering Signals According to Presence or Absence of Chloroform (클로로폼 침적 유무에 따른 후방산란신호 측정 실험 및 분석)

  • Him Chan Seo;Jee Woong Choi;Yongmyung Kim;Moonjin Lee
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.spc
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    • pp.18-22
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    • 2022
  • Because it is difficult to apply direct and optical detection techniques to sunken hazardous and noxious substances (HNS), effective acoustic detection techniques are required to detect sunken HNS in water. In this study, the possibility of acoustic detection of sunken HNS was investigated through backscattering signal measurement experiments using chloroform, a sunken HNS. After establishing a pool in an acrylic tank, backscattering signals were measured according to the presences or absence of chloroform by varying the grazing angle from 90° to 50° in 0.5° intervals using a pan&tilt system. A directional transducer transmitted and received sinusoidal signals with a frequency of 200 kHz and a pulse length of 25 ㎲ in a monostatic state. When chloroform was deposited, the received level of the backscattering signal at the interface between water and chloroform became low at a grazing angle of approximately 80° or smaller. Based on the backscattering signal results obtained at the interface between water and chloroform, the possibility of acoustic detection of sunken HNS was demonstrated.

Hazardous and Noxious Substances (HNSs) Styrene Detection Using Spectral Matching and Mixture Analysis Methods (분광정합 및 혼합 분석 방법을 활용한 위험·유해물질 스티렌 탐지)

  • Jae-Jin Park;Kyung-Ae Park;Tae-Sung Kim;Moonjin Lee
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.spc
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    • pp.1-10
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    • 2022
  • As the volume of marine hazardous and noxious substances (HNSs) transported in domestic and overseas seas increases, the risk of HNS spill accidents is gradually increasing. HNS leaked into the sea causes destruction of marine ecosystems, pollution of the marine environment, and human casualties. Secondary accidents accompanied by fire and explosion are possible. Therefore, various types of HNSs must be rapidly detected, and a control strategy suitable for the characteristics of each substance must be established. In this study, the ground HNS spill experiment process and application result of detection algorithms were presented based on hyperspectral remote sensing. For this, styrene was spilled in an outdoor pool in Brest, France, and simultaneous observation was performed through a hyperspectral sensor. Pure styrene and seawater spectra were extracted by applying principal component analysis (PCA) and the N-Findr method. In addition, pixels in hyperspectral image were classified with styrene and seawater by applying spectral matching techniques such as spectral distance similarity (SDS), spectral correlation similarity (SCS), spectral similarity value (SSV), and spectral angle mapper (SAM). As a result, the SDS and SSV techniques showed good styrene detection results, and the total extent of styrene was estimated to be approximately 1.03 m2. The study is expected to play a major role in marine HNS monitoring.

Evaluation of Flooding Characteristics of Dam Reservoir using Cumulative Flooding Area Curve (누가침수면적곡선을 이용한 댐 저수구역의 침수특성 평가)

  • Munseok Lee;Chulsang Yoo
    • Journal of Wetlands Research
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    • v.25 no.1
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    • pp.14-25
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    • 2023
  • Floodplain in a reservoir is defined as the area naturally formed between the design flood level and the normal pool level. Located around the dam reservoir, floodplain has been damaged in many different ways including cultivation. As it is impossible to restore all the damaged floodplain at once, it is necessary to determine their order of priority. This process considers various factors, among which the flooding frequency is an important hydrologic characteristic, Different from the floodplains in a river, all the floodplains around the given dam reservoir have the same flooding frequency. To overcome this problem, this study proposes to use the cumulative flooding area curve, which represents the cumulative flooding area corresponding to the reservoir water level. Especially, this study evaluates the flooding frequency of those water levels corresponding to the cumulative flooding area of 30%, 50% and 70%. As application examples, this study considers the five restoration candidates each selected in the Andong Dam, Imha Dam, Youngju Dam and Nam river Dam of the Nakdong River Basin. As a result, the cumulative flooding area curve was found to well represent the overall shape of the floodplain (i.e., steep-to-mild slope or mild-to-steep slope). Also, the flooding frequency of those water levels corresponding to the cumulative flooding area of 30%, 50% and 70% was found to be so effective to quantify the hydrologic characteristics of a floodplain.

Stakeholder Awareness of Rural Spatial Planning Data Utilization Based on Survey (농촌공간계획 데이터 수급에 대한 이해당사자 인식조사)

  • Zaewoong Rhee;Sang-Hyun Lee;Sungyun Lee;Jinsung Kim;Rui Qu;Seung-Jong Bae;Soo-Jin Kim;Sangbum Kim
    • Journal of Korean Society of Rural Planning
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    • v.29 no.3
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    • pp.25-37
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    • 2023
  • According to the 「Rural Spatial Reconstruction and Regeneration Support Act」, enacted on March 29, 2024, all local governments are required to establish a 'Rural Spatial Reconstruction and Regeneration Plan' (hereinafter referred to as the 'Rural Spatial Plan'). In order for the 'Rural Spatial Plan' to be appropriately established, this study analyzed the supply and demand of spatial data from the perspective of user stakeholders and derived implications for improving rural spatial planning data utilization. In conclusion, three key recommendations come from this result. Firstly, it is necessary to establish an integrated DB for rural spatial planning data. This can solve the problem of low awareness of scattered data-providing websites, reduce the processing time of non-GIS data, and reduce the time required to acquire data by securing the availability of data search and download. In particular, research should be conducted on the establishment of a spatial analysis simulation system to support stakeholders' decision-making, considering that many stakeholders have difficulty in spatial analysis because spatial analysis techniques were not actively used in rural projects before the implementation of the rural agreement system in 2020. Secondly, research on how to improve data acquisition should be conducted in each data sector. The data sector group with the lowest ease of receiving are 'Local Community Domain', 'Changes in Domestic and International Conditions', and 'Provision and Utilization of Daily Life Services'. Lastly, in-depth research is needed on how to raise each rural spatial planning data supply stakeholder to the position of player. Stakeholders of 'University Institutions' and 'Public Enterprises and Research Institutes' should give those who participate in the formulation of rural spatial plans access to the raw data collected for public work. Stakeholders of 'Private company' need to come up with realistic measures to build a data pool centered on consultative bodies between existing private companies and then prepare a step-by-step strategy to fully open it by participating various stakeholders. In order to induce 'Village Residents and Associations' stakeholders to play a leading role as owners and producers of data, personnel should be trained to collect and record data related to the village. In addition, support measures should be prepared to continue these activities.

Development of Park Performance Assessment Tool (PPAT) for the Qualitative Improvement of Urban Neighborhood Parks (근린공원 서비스의 질적 개선을 위한 공원성능평가도구 개발)

  • Kim, Yong-Gook;Kim, Young-Hyeon;Yoo, Ye-Seul
    • Journal of the Korean Institute of Landscape Architecture
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    • v.51 no.2
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    • pp.120-130
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    • 2023
  • This study aims to develop a Park Performance Assessment Tool (PPAT) to qualitatively evaluate the level of urban neighborhood park services. The Park Performance Assessment Tool (PPAT) development process is as follows. First, a pool of evaluation items was formed by analyzing domestic and foreign cases related to the Park Performance Evaluation Tool (PPAT). Second, evaluation items were refined considering domestic park conditions through expert advisory meetings and pilot evaluations. Third, the reliability of the Park Performance Evaluation Tool (PPAT) was verified through pilot evaluations. Fourth, weights for each evaluation item were derived through an expert survey and AHP analysis. Fifth, a Park Performance Evaluation Tool (PPAT) evaluation table and evaluation guidelines were prepared. The developed Park Performance Assessment Tool (PPAT) was applied to 22 neighborhood parks in Bupyeong-gu, Incheon to evaluate the quality of the physical environment. As a result of the evaluation, several urban neighborhood parks that need improved park services were identified. The Park Performance Evaluation Tool (PPAT) is expected to be used to establish a basic plan for parks and green spaces, select priority and project promotion methods for park maintenance projects, and certify excellent parks

Examination Techniques and Imaging Findings of Hepatic Hemangioma (간혈관종의 검사기법과 영상소견)

  • Chang-Hoe Koo;Jong-Wan Keum;Ji-Eun Seok;Dong-Chul Choi;Yun-Ho Choi;Man-Seok Han;Min-Cheol Jeon
    • Journal of the Korean Society of Radiology
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    • v.17 no.3
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    • pp.375-384
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    • 2023
  • Most Hepatic hemangiomas are asymptomatic and small in size, making them difficult to find by pathological examination. Therefore, radiological diagnosis is essential for the early finding and diagnosis of Hepatic hemangioma. Three-phase method using contrast medium in computed tomography, T1, T2-weighted imaging in magnetic resonance imaging, dynamic magnetic resonance imaging using contrast medium, echo planar imaging method, diffusion-weighted imaging method, blood pool scan using 99mTc-labeled red blood cells in nuclear medicine, we looked at the color doppler method In ultrasound, and it is important to accurately understand the imaging findings of hepatic hemangioma and perform the examination in order to make an accurate diagnosis. most hepatic hemangioma are benign tumors, care should be taken not to confuse them with malignant tumors such as hepatocellular carcinoma to prevent unnecessary procedures. Therefore, in order to make an accurate diagnosis, it is important to accurately understand the imaging findings of hemangioma and perform the examination.

Preferences and Consumption Patterns of General Consumers of Meat Dishes (일반소비자들의 고기 요리에 대한 기호도 및 소비행태)

  • Kim, Eun-Mi;Seo, Sang-Hee;Lee, Min-A;Kwon, Ki-Hyun;Jun, Gi-Hong
    • Journal of the Korean Society of Food Culture
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    • v.25 no.3
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    • pp.251-261
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    • 2010
  • This paper examines the dining tendencies of Koreans toward meat dishes and their related factors, with 301 people residing in Korea as subjects. We questioned this population on their restaurant choices, eating habits, preferences and a dislike with respect to method of cooking. All age groups, with the exception of 50~60 year-olds, were found to consume meat dishes in specialized restaurants. The selection factor for restaurants varied, depending on the age group: affordable cost was the most important factor for whom under 20 year-old. Whereas 20-40 year-old people considered taste and ambience the most for choosing restaurant. The 50-60 year-old people found to seek for convenient restaurant. In terms of the most preferred parts of beef, 20-30 year-old people preferred tenderloin (42.3%) while 40-60 year-old people preferred sirloin (45.6% and 41.2% each). All the other age groups preferred ribs the most (It was also found that) men preferred the rib parts and women preferred tenderloin. With respect to pork, all age groups preferred pork belly, with the highest response rate in the under 20 age group. When consuming meat dishes, 91.0% of respondents pointed to roasting as the most frequent preparation method, the reason being good taste was deemed the highest number of responses. In a survey on preference for stewed meats, 74.8% of the sample pool was found to prefer stew dishes but 24.9% of respondents provided negative answers, the reason for dislike was found to be poor taste for women, and both a long preparation time and poor taste for men. In fact, regardless of marital status, a high number of responses were given for poor taste as the reason for disliking stew dishes. Based on the examined factors for inconveniences in preparing meat dishes, the most common inconvenience factor was the odor of meat left on clothes after the dining experience, followed by the smoke generated in roasting meat, and the safety risk involved with changing the grill. In terms of stir fry preparation, the negative thoughts were due to the splashing of food and spices while cooking, followed by the meat odor left on clothes after dining experience, and the sanitation level of the cooking containers. For deep frying the sanitation level of cooking containers was the biggest reason for dislike, followed by splashing of food when preparing. Lastly, it was indicated that the problem of steaming method was a difficulty of judging the portion, on the top of this, people found that the sanitation level of cooking container and a long preparation time were also the problem of this cooking method.

Changes in the Riverbed Landforms Due to the Artificial Regulation of Water Level in the Yeongsan River (인위적인 보 수위조절로 인한 영산강 하도 지형 변화)

  • Lim, Young Shin;Kim, Jin Kwan
    • Journal of The Geomorphological Association of Korea
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    • v.27 no.1
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    • pp.1-19
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    • 2020
  • A river bed which is submerged in water at high flow and becomes part of the river at low flow, serves as a bridge between the river and the land. The channel bar creates a unique ecosystem with vegetation adapted to the particular environment and the water pool forms a wetland that plays a very important role in the environment. To evaluate anthropogenic impacts on the river bed in the Middle Yeongsangang River, the fluvial landforms in the stream channel were analyzed using multi-temporal remotely-sensed images. In the aerial photograph of 2005 taken before the construction of the large weirs, oxbow lakes, mid-channel bars, point bars, and natural wetlands between the artificial levees were identified. Multiple bars divided the flow of stream water to cause the braided pattern in a particular section. After the construction of the Seungchon weir, aerial photographs of 2013 and 2015 revealed that most of the fluvial landforms disappeared due to the dredging of its riverbed and water level control(maintenance at 7.5El.m). Sentinel-2 images were analyzed to identify differences between before and after the opening of weir gate. Change detection was performed with the near infrared and shortwave infrared spectral bands to effectively distinguish water surfaces from land. As a result, water surface area of the main stream of the Yeongsangang River decreased by 40% from 1.144km2 to 0.692km2. A large mid-channel bar that has been deposited upstream of the weir was exposed during low water levels, which shows the obvious influence of weir on the river bed. Newly formed unvegetated point bars that were deposited on the inside of a meander bend were identified from the remotely sensed images. As the maintenance period of the weir gate opening was extended, various habitats were created by creating pools and riffles around the channel bars. Considering the ecological and hydrological functions of the river bed, it is expected that the increase in bar areas through weir gate opening will reduce the artificial interference effect of the weir.

An Exploratory Study on ChatGPT's Performance to Answer to Police-related Traffic Laws: Using the Driver's License Test and the Road Traffic Accident Appraiser (ChatGPT의 경찰 관련 교통법규 응답 능력에 대한 탐색적 연구 - 운전면허 학과시험과 도로교통사고감정사 1차 시험을 대상으로 -)

  • Sang-yub Lee
    • Journal of Digital Policy
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    • v.2 no.4
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    • pp.1-10
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    • 2023
  • This study conducted preliminary study to identify effective ways to use ChatGPT in traffic policing by analyzing ChatGPT's responses to the driver's license test and the road traffic accident appraiser test. I collected ChatGPT responses for the driver's license test item pool and the road traffic accident appraiser test using the OpenAI API with Python code for 30 iterative experiments, and analyzed the percentage of correct answers by test, year, section, and consistency. First, the average correct answer rate for the driver's license test and the for road traffic accident appraisers test was 44.60% and 35.45%, respectively, which was lower than the pass criteria, and the correct answer rate after 2022 was lower than the average correct answer rate. Second, the percentage of correct answers by section ranged from 29.69% to 56.80%, showing a significant difference. Third, it consistently produced the same response more than 95% of the time when the answer was correct. To effectively utilize ChatGPT, it is necessary to have user expertise, evaluation data and analysis methods, design a quality traffic law corpus and periodic learning.