• KSBB Journal
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• v.21 no.3
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• pp.204-211
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• 2006

For the production of the recombinant human interferon-gamma(rhIFN-${\gamma}$) in Escherichia coli, human glucagon and ferritin heavy chain were used as fusion partners. Even though rhIFN-${\gamma}$ is expressed as an inclusion body form in E. coli because of strong hydrophobicity of itself, over 50% of fused rhIFN-${\gamma}$ was expressed as soluble form in E. coli $Origami^{TM}$(DE3) harboring pT7FH(HE)-IFN-${\gamma}$ which encodes ferritin heavy chain-fused rhIFN-${\gamma}$. In the case of using glucagon-ferritin heavy chain hybrid mutant as a fusion partner, 6X His-tag was additionally introduced to N-terminus of GFHM(HE)-IFN-${\gamma}$ for enhancing purification yields of rhIFN-${\gamma}$. Fusion protein HGFHM(HE)-IFN-${\gamma}$ with two 6X His-tag was more effectively bound to Ni-NTA agarose bead than GFHM(HE)-IFN-${\gamma}$ with a 6X His-tag. rhIFN-${\gamma}$ was completely purified from enterokinase-treated HGFHM(HE)-IFN-${\gamma}$ by Ni-NTA affinity column. For high-level production of rhIFN-${\gamma}$, glucose was used as the sole carbon source with simple exponential feeding rate($2.4{\sim}7.2g/h$) in fed-batch process. The effective lactose concentration for the expression of the rhIFN-${\gamma}$ was $10{\sim}20mM$. Under the fed-batch culture conditions, rhIFN-${\gamma}$ production yield reached 11 g DCW/L for 6 hours after lactose induction.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.256-266
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• 2022

Empirical models including the Angstrom-Prescott (AP) model have been used to estimate solar radiation at sites, which would support a wide use of crop models. The objective of this study was to estimate two sets of solar radiation estimates using the AP coefficients derived for climate zone (AP_Frere) and specific site (AP_Choi), respectively. The daily solar radiation was estimated at 18 sites in Korea where long-term measurements of solar radiation were available. In the present study, daily solar radiation and sunshine duration were collected for the period from 2012 to 2021. Daily weather data including maximum and minimum temperatures and rainfall were also obtained to prepare input data to a process-based crop model, CERES-Rice model included in Decision Support System for Agrotechnology Transfer (DSSAT). It was found that the daily estimates of solar radiation using the climate zone specific coefficient, SFrere, had significantly less error than those using site-specific coefficients S_Choi (p<0.05). The cumulative values of S_Frere for the period from march to September also had less error at 55% of study sites than those of S_Choi. Still, the use of S_Frere and S_Choi as inputs to the CERES-Rice model resulted in slight differences between the outcomes of crop growth simulations, which had no significant difference between these outputs. These results suggested that the AP coefficients for the temperate climate zone would be preferable for the estimation of solar radiation. This merits further evaluation studies to compare the AP model with other sophisticated approaches such as models based on satellite data.

• Journal of Nutrition and Health
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• v.48 no.2
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• pp.180-191
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• 2015

Purpose: There is a lack of data on the energy cost of children's everyday activities, adult values are often used as surrogates. In addition, the influence of body weight on the energy cost of activity when expressed as metabolic equivalents (METs) has not been vigorously explored. Methods: In this study 20 elementary school students 9~12 years of age completed 18 various physical activities while energy expenditure was measured continuously using a portable telemetry gas exchange system ($K_4b^2$, Cosmed, Rome, Italy). Results: The average age was 10.4 years and the average height and weight was 145.1 cm and 43.6 kg, respectively. Oxygen consumption ($VO_2$), energy expenditure and METs at the time of resting of the subjects were 5.41 mL/kg/min, 1.44 kcal/kg/h, and 1.5 METs, respectively. METs values by 18 physical activities were as follows: Homework and reading books (1.6 METs), playing game with a mobile phone or video while sitting (1.6 METs), watching TV while sitting on a comfortable chair (1.7 METs), playing video game or mobile phone game while standing (1.9 METs), sweeping a room with a broom (2.7 METs) and playing a board game (2.8 METs) belong to light intensity physical activities. By contrary, speedy walking and running were 6.6 and 6.7 METs, respectively, which belong to high intensity physical activities over 6.0 METs. When the effect of body weight on physical activity energy expenditure was determined, $R^2$ values increased with 0.116 (playing a game at sitting), 0.176 (climbing up and down stairs), 0.246 (slow walking), and 0.455 (running), which showed that higher activity intensity increased explanation power of body weight on METs value. Conclusion: This study is important for direct evaluation of energy expenditure by physical activities of children, and it could be used directly for revising and complementing the existing activity classification table to fit for children.

• Nuclear Medicine and Molecular Imaging
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• v.42 no.3
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• pp.235-245
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• 2008

Purpose: The HSV1-tk gene has been extensively studied as a type of reporter gene. In hepatocellular carcinoma (HCC), only a small proportion of patients are eligible for surgical resection and there is limitation in palliative options. Therefore, there is a need for the development of new treatment modalities and gene therapy is a leading candidate. In the present study, we investigated the usefulness of substrate, 2'-fluoro-2'-deoxy-1-${\beta}$-D-arabino-furanosyi-5-[$^{124/125}I$]iodo- uracil ([$I^{124/125}I$]FIAU) as a non-invasive imaging agent for HSV1-tk gene therapy in hepatoma model using small animal PET. Material and Methods: With the Morris hepatoma MCA cell line and MCA-tk cell line which was transduced with the HSV1-tk gene, in vitro uptake and correlation study between [$^{125}I$]FIAU uptake according to increasing numeric count of percentage of MCA-tk cell were performed. The biodistribution data and small animal PET images with [$^{124}I$]FIAU were obtained with Balb/c-nude mice bearing both MCA and MCA-tk tumors. Results:, Specific accumulation of [[$^{125}I$]FIAU was observed in MCA-tk cells but uptake was low in MCA cells. Uptake in MCA-tk cells was 15 times higher than that of MCA cells at 480 min. [$^{125}I$]FIAU uptake was linearly correlated (R2 =0.964, p =0.01) with increasing percentage of MCA-tk numeric cell count. Biodistribution results showed that [$^{125}I$]FIAU was mainly excreted via the renal system in the early phase. Ratios of MCA-tk tumor to blood acting were 10, 41, and 641 at 1 h, 4 h, and 24 h post-injection, respectively. The maximum ratio of MCA-tk to MCA tumor was 192.7 at 24 h. Ratios of MCA-tk tumor to liver were 13.8, 66.8, and 588.3 at 1 h, 4 h, and 24 h, respectively. On small animal PET, [$^{124}I$]FIAU accumulated in substantial higher levels in MCA-tk tumor and liver than MCA tumor. Conclusion: FIAU shows selective accumulation to HSV1-tk expressing hepatoma cell tumors with minimal uptake in normal liver. Therefore, radiolabelled FIAU is expected to be a useful substrate for non-invasive imaging of HSV1-tk gene therapy and therapeutic response monitoring of HCC.

• Journal of the Korean Institute of Landscape Architecture
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• v.41 no.6
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• pp.20-28
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• 2013

The purpose of this study was to compare the ultra-violet(UV) radiation under the landscaping shade facilities and tree with natural solar UV of the outdoor space at summer middays. The UVA+B and UVB were recorded every minute from the $20^{th}$ of June to the $26^{th}$ of September 2012 at a height of 1.1m above in the four different shading conditions, with fours same measuring system consisting of two couple of analog UVA+B sensor(220~370nm, Genicom's GUVA-T21GH) and UVB sensor(220~320nm, Genicom's GUVA-T21GH) and data acquisition systems(Comfile Tech.'s Moacon). Four different shading conditions were under an wooden shelter($W4.2m{\times}L4.2m{\times}H2.5m$), a polyester membrane structure ($W4.9m{\times}L4.9m{\times}H2.6m$), a Salix koreensis($H11{\times}B30$), and a brick-paved plot without any shading material. Based on the 648 records of 17 sunny days, the time serial difference of natural solar UVA+B and UVB for midday periods were analysed and compared, and statistical analysis about the difference between the four shading conditions was done based on the 2,052 records of daytime period from 10 A.M. to 4 P.M.. The major findings were as follows; 1. The average UVA+B under the wooden shelter, the membrane and the tree were $39{\mu}W/cm^2$(3.4%), $74{\mu}W/cm^2$(6.4%), $87{\mu}W/cm^2$(7.6%) respectively, while the solar UVA+B was $1.148{\mu}W/cm^2$. Which means those facilities and tree screened at least 93% of solar UV+B. 2. The average UVB under the wooden shelter, the membrane and the tree were $12{\mu}W/cm^2$(5.8%), $26{\mu}W/cm^2$(13%), $17{\mu}W/cm^2$(8.2%) respectively, while the solar UVB was $207{\mu}W/cm^2$. The membrane showed the highest level and the wooden shelter lowest. 3. According to the results of time serial analysis, the difference between the three shaded conditions around noon was very small, but the differences of early morning and late afternoon were apparently big. Which seems caused by the matter of the formal and structural characteristics of the shading facilities and tree, not by the shading materials itself. In summary, the performance of the four landscaping shade facilities and tree were very good at screening the solar UV at outdoor of summer middays, but poor at screening the lateral UV during early morning and late afternoon. Therefore, it can be apparently said that the more delicate design of shading facilities and big tree or forest to block the additional lateral UV, the more effective in conditioning the outdoor space reducing the useless or even harmful radiation for human activities.

• Journal of Intelligence and Information Systems
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• v.21 no.3
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• pp.1-17
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• 2015

In this paper, we report what we have observed with regards to a prediction model for the military based on enlisted men's internal(cumulative records) and external data(SNS data). This work is significant in the military's efforts to supervise them. In spite of their effort, many commanders have failed to prevent accidents by their subordinates. One of the important duties of officers' work is to take care of their subordinates in prevention unexpected accidents. However, it is hard to prevent accidents so we must attempt to determine a proper method. Our motivation for presenting this paper is to mate it possible to predict accidents using enlisted men's internal and external data. The biggest issue facing the military is the occurrence of accidents by enlisted men related to maladjustment and the relaxation of military discipline. The core method of preventing accidents by soldiers is to identify problems and manage them quickly. Commanders predict accidents by interviewing their soldiers and observing their surroundings. It requires considerable time and effort and results in a significant difference depending on the capabilities of the commanders. In this paper, we seek to predict accidents with objective data which can easily be obtained. Recently, records of enlisted men as well as SNS communication between commanders and soldiers, make it possible to predict and prevent accidents. This paper concerns the application of data mining to identify their interests, predict accidents and make use of internal and external data (SNS). We propose both a topic analysis and decision tree method. The study is conducted in two steps. First, topic analysis is conducted through the SNS of enlisted men. Second, the decision tree method is used to analyze the internal data with the results of the first analysis. The dependent variable for these analysis is the presence of any accidents. In order to analyze their SNS, we require tools such as text mining and topic analysis. We used SAS Enterprise Miner 12.1, which provides a text miner module. Our approach for finding their interests is composed of three main phases; collecting, topic analysis, and converting topic analysis results into points for using independent variables. In the first phase, we collect enlisted men's SNS data by commender's ID. After gathering unstructured SNS data, the topic analysis phase extracts issues from them. For simplicity, 5 topics(vacation, friends, stress, training, and sports) are extracted from 20,000 articles. In the third phase, using these 5 topics, we quantify them as personal points. After quantifying their topic, we include these results in independent variables which are composed of 15 internal data sets. Then, we make two decision trees. The first tree is composed of their internal data only. The second tree is composed of their external data(SNS) as well as their internal data. After that, we compare the results of misclassification from SAS E-miner. The first model's misclassification is 12.1%. On the other hand, second model's misclassification is 7.8%. This method predicts accidents with an accuracy of approximately 92%. The gap of the two models is 4.3%. Finally, we test if the difference between them is meaningful or not, using the McNemar test. The result of test is considered relevant.(p-value : 0.0003) This study has two limitations. First, the results of the experiments cannot be generalized, mainly because the experiment is limited to a small number of enlisted men's data. Additionally, various independent variables used in the decision tree model are used as categorical variables instead of continuous variables. So it suffers a loss of information. In spite of extensive efforts to provide prediction models for the military, commanders' predictions are accurate only when they have sufficient data about their subordinates. Our proposed methodology can provide support to decision-making in the military. This study is expected to contribute to the prevention of accidents in the military based on scientific analysis of enlisted men and proper management of them.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.100-100
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• 2003

양식 산업에 있어 사육 생물의 빠른 성장은 가장 요구되는 특성이다. 특히, 선발육종을 통한 성장 개선은 사료의 효율적 이용과 연관되어 있으며, 균형 잡힌 단백질 대사 등을 통해 이루어진다. 대부분의 경골어류는 단백질 대사의 마지막 부산물로 암모니아를 생성하여 배설하며, 이를 통한 사료 내 단백질의 효율적 이용성을 비교하는 척도로 제시되어 왔다(Ming, 1985). 따라서 본 실험에서는 한국해양연구원에서 선발육종 해 온 참돔과 일본 양식산 참돔 및 이들의 교배 자손들을 대상으로 일간 먹이 공급, 절식 그리고 1회 만복 공급에 따른 암모니아성 질소 배설률을 조사하였으며, 일간 먹이 공급에 따른 각 교배 자손들의 분 배출 특성을 알아보았다. 실험어는 일본 양식산인 JPN 교배구 자손과 한국해양연구원 선발육종산인 KORDI F4 교배구 자손, 그리고 JPN$\times$KORDI F4♂ 교배구 자손을 대상으로 실시하였다. 체중이 각각 17.1$\pm$0.1 g (JPN 교배구; 그룹 1), 17.7$\pm$0.1 g (JPN♀$\times$KORDI F4♂; 그룹 2), 21.5$\pm$0.1 g (KORDI F4; 그룹 3)인 참돔 치어를 각각 15마리씩 3반복 수용하여 실험에 이용하였다. 실험어는 지름이 33 cm이고 높이가 34 cm인 둥근 투명 플라스틱 수조에 수용하고 분 수집기인 TUF column (passing effluent water through a special test tube) system을 이용 어체중의 3%에 해당하는 사료 량을 일간 세 번에 나누어 동일량을, 09시부터 17시까지 4시간 간격으로 공급한 14일째에 일간 먹이 공급에 따른 24시간 동안의 참돔 종간 잡종 치어의 총암모니아성 질소(total ammonia nitrogen, TAN) 배설률 및 분 배출률을 조사하였다. 이 조사가 끝난 후 3일간 절식시킨 다음 내인성 암모니아성 질소 배설을 조사하였으며, 이어서 1회 만복 사료 공급한 다음 이에 따른 암모니아성 질소 배설 경향을 알아보았다. 일간 먹이 공급에 따른 각 실험구의 암모니아 배설은 세 가지 계통 참돔치어 모두 먹이 섭취 후 암모니아 배설로 인한 TAN 농도가 지속적으로 증가하는 경향을 보였으며, 시간당 TAN 배설률을 적분한 결과, 그룹 1과 2 그리고 3의 일간 TAN 배설률은 각각 637.3$\pm$36.5 mg/kg fish/day, 684.3$\pm$18.5 mg/kg fish/day 그리고 772.8$\pm$17.3 mg/kg fish/day로 나타나 그룹 3의 일간 TAN 배설률이 가장 높은 결과를 보였다. 3일간 절식 후 그룹 1, 2, 그리고 2의 TAN 배설률은 각각 8.08~14.15 mg/kg fish/hr, 6.50~12.20 mg/kg fish/hr, 그리고 6.67~8.60 mg/kg fish/hr의 범위를 보여 거의 일정한 농도로 나타났다. 시간당 TAN 배설률을 적분한 결과, 그룹 1, 2 그리고 3의 일간 TAN 배설률은 각각 286.9$\pm$28.3 mg/kg fish/day, 215.7$\pm$5.5 mg/kg fish/day 그리고 179.3$\pm$7,7 mg/kg fish/day로 나타나 먹이 공급과는 달리 내인성 TAN 배설의 경우 그룹 1의 배설률이 가장 높은 결과를 보였다. 1회 만복 먹이 공급에 따른 TAN 배설 경향은 그룹 1과 2의 TAN 배설률은 먹이 공급 6시간 후 가장 높은 값인 44.19$\pm$2.90 mg/kg fish/hr와 41.70$\pm$1.40 mg/kg fish/hr을 보였고, 그룹 3에서는 4시간 후에 가장 높은 값인 31.23$\pm$1.39 mg/kg fish/day로 나타났다. 일간 먹이 공급에 따른 각 실험구의 총 분 배출량은 그룹 1의 경우 총 분 배출량은 2.17$\pm$0.1 g/kg fish와 91.15$\pm$4.53 g/kg feed로 나타났고, 그룹 2에서는 2.26$\pm$0.14 g/kg fish와 95.02$\pm$3.18 g/kg feed, 그룹 3에서는 2.81$\pm$0.73 g/kg fish와 132.85$\pm$34.0 g/kg feed로 나타나 유의적인 차이는 보이지 않았으나(P>0.05), 그룹 3의 배출 비율이 높은 것으로 나타났다. 시간 경과에 따른 분 배출 비율은 그룹 1의 경우 먹이 공급 24시간 후 총 분의 60.6%를 보여 반면, 그룹 2와 3에서는 각각 67.8%와 77.8%를 보여 그룹 1이 다른 그룹에 비해 분 배출 비율이 낮은 것으로 나타났다.

• Journal of Life Science
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• v.23 no.9
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• pp.1155-1162
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• 2013

Estrogen like activities were evaluated using ethanol and hot water extracts of gongjindan (GJD) and 3 types of brown algae by an in vitro detection system. Laminaria japonica and Undaria pinnatifida represented statistically significant (p<0.001) estrogen like activities, while GJD and Ecklonia stolonifera did not. Furthermore, activities of hot water extracts ($500{\mu}g/ml$) of Laminaria japonica and three mixture (Ecklonia stolonifera : Undaria pinnatifida : Laminaria japonica, 1: 1: 1) were stronger than that of $10^{-7}M$ $17{\beta}$-estradiol. These results suggest that Laminaria japonica contain estrogen like compounds. Based on these results, the prepared seaweed GJD were used for animal experiments about the effects on inhibition of platelet aggregation and serum lipid levels in ovariectomized rats. 9-week Old female SD-rats were randomly assigned to 3 groups as sham-operated rats, ovariectomized rats and ovariectomized rats that were treated with GJD. The rats were placed on prescribed diets for 5 weeks following ovariectomy. Total cholesterol and triglyceride contents on serum decreased in the SHAM group compared to the OVX-CON group. 5 weeks feeding of GJD resulted in significant lowering of triglyceride and a decreasing tendency of total cholesterol level. The level of HDL-cholesterol on serum increased significantly by feeding diets containing the GJD. The ability of platelet aggregation of group treated with GJD was less than that of the OVX-CON group. These results suggest that the beneficial effects of seaweed gongjindan may be used to improve menopausal syndrome.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.392-399
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• 2013

This study aimed to investigate responses of photosynthesis, plant growth, and phytochemical contents to different artificial light sources for 'Seneca RZ' and 'Gaugin RZ' two butterhead lettuce (Lactuca sativa L.). In this study, fluorescent lamps (FL), three colors LEDs (red, blue and white, 5 : 4 : 1; RBW) and metalhalide lamps (MH) were used as artificial lighting sources. Photoperiod, air temperature, relative humidity, EC, and pH in a cultivation system were maintained at 16/8 h, $25/15^{\circ}C$, 60~70%, $1.4{\pm}0.2dS{\cdot}m^{-1}$, and $6.0{\pm}0.5$, respectively. The photosynthetic rate of both two butterhead lettuce were the highest under RBW in middle growth stage. However, in late growth stage, the photosynthetic rate of both two butterhead lettuce were higher under RBW and MH than FL. The light sources showed significant results for plant growth but those effects were different to variety. Fresh and dry weight of 'Gaugin RZ' butterhead lettuce under MH were heavier than other lights in all growth stages. Growth of 'Seneca RZ' butterhead lettuce was maximized highest under MH in middle growth stage and FL in late growth stage. In the leaf tissue of 'Seneca RZ' butterhead lettuce, tipburn symptom occurred under all light sources and in the leaf tissue of 'Gaugin RZ' butterhead lettuce, it occurred under two light sources except for fluorescent lamps in late growth stage. kinds of lamp affect plant growth more than plant quality. Relative growth rate of both two butterhead lettuce was faster in middle growth stage than late stage. Growth of 'Gaugin RZ' was shown by kinds of lamp in middle growth stage and but it was not significantly affected by light sources and variety in late stage. Most of the phytochemical contents of two butterhead lettuce were significantly affected by different light sources. Contents of all vitamins showed higher than other light sources on RBW for both two lettuce, especially ${\beta}$-Carotene content of 'Gaugin RZ' was the highest. Plant growth, photosynthesis, and phytochemical contents were observed significant effects by different light sources for two butterhead lettuce but those effects were highly different between variety and kinds of phytochemicals. Therefore, the selection of optimum light source should be considered by variety and kinds of phytochemicals in the plant factory.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.16 no.2
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• pp.199-210
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• 2005

Objective : Attention deficit hyperactivity disorder(ADHD) affects $5-10\%$ of children in Korea, with more boys and girls being diagnosed. Despite seriousness of ADHD, little is known about its causes. From the current genetic epidemiologic studies, ADHD is known as a heritable disorder. Till now, however, there have been very few genetic studies about ADHD in Korea. The aim of the this study is to examine the association between dopamine transporter gone type 1 and ADHD using case-control design in Korean ADHD probands and normal controls. Materials and Method : Child Psychiatric Genetic research team in Seoul National University Hospital, Clinical Research Institute recruited the ADHD probands using clinical interview/observation, diverse rating scales, and neuropsychological tests. For eliminating phenocopy or ADHD, diagnosis of ADHD was based upon clinical data, psychometric data, and parent/teacher reports. Total 85 ADHD-probands were recruited as final study subjects and independent 100 normal adults participated in this study as control group. For all the ADHD probands, and controls, the 3'-UTR-VNTR polymorphism of DAT1 was analyzed. Based on the DAT1 allele and genotype informations, Chi-square test based on case-control design was performed. Results : As for genetic study, total of 85 probands and 100 controls were included for the genetic analysis. Four different alleles, 350bp (7repeat), 440bp (9repeat), 480bp (10repeat) and 520bp (11repeat) were found in DAT1 gene of study subjects. In case-control analysis, ADHD probands and parents have significantly more 9 repeat allele and 9/10 genotype. Also, The probands with 9repeat allele have more commission errors in ADS. Conclusion : The positive association between ADHD and DAT1 gene was replicated in this report like other previous results for caucasian children and Korean children with ADHD. There are ongoing studies on other candidate genes such as DRD4 and DRD5 and it would be required to explore the association of these candidate genes in Korean children with ADHD. These ongoing genetic research will contribute to the understanding of heterogenous genetic and environmental etiologies of ADHD phenotype, which will lead to the development of more comprehensive treatment and preventive interventions for ADHD.