• Journal of Photoscience
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• v.9 no.3
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• pp.45-48
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• 2002

Microbial rhodopsins, photoactive 7-transmembrane helix proteins that use retinal as their chromophore, were observed initially in the Archaea and appeared to be restricted to extreme halophilic environments. Our understanding of the abundance and diversity of this family has been radically transformed by findings over the past three years. Genome sequencing of cultivated microbes as well as environmental genomics have unexpectedly revealed archaeal rhodopsin homologs in the other two domains of life as well, namely Bacteria and Eucarya. Organisms containing these homologs inhabit such diverse environments as salt flats, soil, freshwater, and surface and deep ocean waters, and they comprise a broad phylogenetic range of microbial life, including haloarchaea, proteobacteria, cyanobacteria, fungi, and algae. Analysis of the new microbial rhodopsins and their expression and structural and functional characterization reveal that they fulfill both ion transport and sensory functions in various organisms, and use a variety of signaling mechanisms. We have obtained the first crystallographic structure for a photosensory member of this family, the phototaxis receptor sensory rhodopsin II (SRII, also known as phoborhodopsin) that mediates blue-light avoidance by the haloarchaeon Natronobacterium pharaonis. The structure obtained from x-ray diffraction of 3D crystals prepared in a cubic lipid phase reveals key features responsible for its spectral tuning and its sensory function. The mechanism of SRII signaling fits a unified model for transport and signaling in this widespread family of phototransducers.

• Molecules and Cells
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• v.27 no.4
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• pp.449-458
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• 2009

The OsAsr1 cDNA clone was isolated from a cDNA library prepared from developing seed coats of rice (Oryza sativa L.). Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs. In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma. For transgenic rice plants that over-expressed full-length OsAsr1 cDNA in the sense orientation, the Fv/Fm values for photosynthetic efficiency were about 2-fold higher than those of wild type-segregating plants after a 24-h cold treatment. Seedlings exposed to prolonged low temperatures were more tolerant of cold stress, as demonstrated during wilting and regrowth tests. Interestingly, OsAsr1 was highly expressed in transgenic rice plants expressing the C-repeat/dehyhdration responsive element binding factor 1 (CBF1), suggesting the regulation of OsAsr1 by CBF1. Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance.

• Journal of Life Science
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• v.12 no.1
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• pp.67-76
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• 2002

The effect of transgene inactivation in T2, T3 and F2 generations was analyzed in progeny seedlings which had been generated by Agrobacterium (LBA4404/pBI121)-mediated transformation in Arabidopsis thaliana. In a system which investigated in the expression of $\beta$-glucuronidase(GUS)gene in kanamycin-resistant (ke $n^{R}$)seedlings, GUS inactivated seedlings were observed in 5 of 12 tested lines of T2 generation and the frequency of GUS inactivation was approximately 2.3%. Lines with multi-copies of T-DNA exhibited severe GUS gene inactivation with the frequency of 5.8% in T2 generation. In T3 generation lines exhibited GUS gene inactivation with the frequency of 1.3%. In contrast, inactivation increased dramatically up to 12.6% in multi-copy T-DNA line. A similar phenomenon was also found in F2 progeny from a transgenic line which had been crossed with wild-type Arabidopsis plant, WS-O (GUS gene inactivation frequency 9.9%). These results indicate that the foreign gene introduced into the plant was inactivated progressively in its transmission during subsequent generations and the transgenic line with multi-copies of T-DNA tended to show more increased inactivation.

• Restorative Dentistry and Endodontics
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• v.23 no.1
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• pp.223-235
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• 1998

The recognition of the esthetics as well as their function of the teeth in the dental field is growing. Evaluating the esthetics of a tooth concerns shape, texture, and color. And among these, color has been the most difficult element to make natural expression. Color is a very subjective element affected by many different factor like light source, object, observer. Numerous efforts to quantifying color has been made. Currently the use of colorimeter and spectrophotometercolor that the color measuring device has been suggested. In this study, the normal range of the tooth color in canines, lateral incisors and central incisors for Koreans was evaluated with a modified spectrophotometer for intraoral use. The color differences in terms of teeth positions, age and area within the tooth was evaluated and comparatively analyzed. Results were as follow ; 1. In all age groups, the central incisors are most bright and least yellowish opposit to the canines are most dark and yellowish. The central incisors and lateral incisors showed the similar color patterns. 2. In the comparison between gender, it showed that in the 10' s group male have more yellowish, the 20's female have lighter and more redness, the 30's indicated more redness in female and in the 40's the lightness is more in female. 3. With aging, there was no significant change in $L^*$ and $a^*$ coordinates but a shift was in $b^*$ to yellowish in the 30' sand 40's in central incisors. Lateral incisors and canines showed a lighter and more yellowish tooth color with an irregular change of color coordinate in $a^*$. 4. In all teeth the middle and cervical portion showed close correlation with over 0.7(0.05>p) but color ($a^*$, $b^*$) showed higher correlation than lightness($L^*$). 5. It has been shown that through the regression test the cervical color could be calculated by the middle color value.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.3
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• pp.209-218
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• 2013

Early researches in human action recognition have focused on tracking and classifying articulated body motions. Such methods required accurate segmentation of body parts, which is a sticky task, particularly under realistic imaging conditions. Recent trends of work have become popular towards the use of more and low-level appearance features such as spatio-temporal interest points. Given the great progress in pose estimation over the past few years, redefined views about pose-based approach are needed. This paper addresses the issues of whether it is sufficient to train a classifier only on low-level appearance features in appearance approach and proposes effective pose-based approach with pose estimation for emotional action recognition. In order for these questions to be solved, we compare the performance of pose-based, appearance-based and its combination-based features respectively with respect to scenario of various emotional action recognition. The experiment results show that pose-based features outperform low-level appearance-based approach of features, even when heavily spoiled by noise, suggesting that pose-based approach with pose estimation is beneficial for the emotional action recognition.

• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.5
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• pp.674-682
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• 2009

The modular robot is one which was developed to get over limit of the space movement for the mobile robot. The chain type robot in particular is connected by series each other and this form expression method is simple and easy to really make a docking method efficiently. However, the related studies were focused on the movement that used to be combination, and the movement of a cell independent mainly does not consist and have a problem to dock only in a direction, not to be connected with all directions. Therefore, we suggested a modular structure for quick, independent movement to solve such a problem and had own autonomy. In addition, we are intended to get some effectiveness for connection mechanism using one locking motor. In this paper, we dealt with the design for the mechanical and electrical points and docking algorithm including communication method. All of the structure is verified with real action experiment through the shape expressions of various application platform.

• Korean Journal of Remote Sensing
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• v.26 no.5
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• pp.537-547
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• 2010

The importance of vegetation in studies of global climate and biogeochemical cycles is well recognized. Especially. the FPAR (fraction of photosynthetically active radiation) is one of the important parameters in ecosystem productivity and carbon budget models. Therefore, accurate estimates of vegetation parameters are increasingly important in environmental impact assessment studies. In this study, optical FPAR using the Terra MODIS (MODerate resolution Imaging Spectroradiometer), SPOT VEGETATION and ECOCLIMAP data reproduced on the Korean peninsula. We applied the empirical method which is usually estimated as a linear or nonlinear function of vegetation indices. As results, we estimated the accurate expression which is 0.9039 of $R^2$ in cropland and 0.7901 of $R^2$ in forest. Finally, this study could be demonstrated to calibrate that produced FPAR while the overall pattern and random noise through the comparative analysis of FPAR on the reference data. Optimal use of input parameter on the Korean peninsula should be helping the accuracy of output as well as the improved quality of research.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.481-487
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• 2010

The ripening behavior of three apple cultivars, 'Tsugaru', 'Hongro' and 'Fuji' was distinctive and the involvement of POLYGALACTURONASE(PG) in the fruit softening process was confirmed to be ethylene dependent. Fruit softening is genetically coordinated by the action of several cell wall enzymes, including PG which depolymerizes cell wall pectin. Also, loss of firmness is associated with increasing of the ripening hormone, ethylene. In this work, climacteric ripening of three apple cultivars, Tsugaru, Hongro and Fuji, producing different ethylene levels and ripening responses, was examined. Correspondingly in Fuji, a linear and basal ethylene level was observed over the entire period of measurements, and Tsugaru and Hongro displayed a typical climacteric rise in ethylene production. Transcript accumulation of genes involved in ethylene biosynthesis (MdACS3 and MdACO1) and MdPG1 was studied in Tsugaru, Hongro and Fuji cultivars. Expression of MdACO1 transcripts was shown in all three ripened apple fruits. However, the MdACS3 and MdPG1 were transcribed differently in these cultivars. Comparing the MdPG1 of 'Tsugaru', 'Hongro' and 'Fuji', structural difference was discovered by genomic Southern analysis. Overall results pointed out that MdACS3 and MdPG1 play an important role in regulation of fruit ripening in apple cultivar.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.86-94
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• 2019

Background: Ginseng is believed to have antitumor activity. Autophagy is largely a prosurvival cellular process that is activated in response to cellular stressors, including cytotoxic chemotherapy; therefore, agents that inhibit autophagy can be used as chemosensitizers in cancer treatment. We examined the ability of Korean Red Ginseng extract (RGE) to prevent autophagic flux and to make hepatocellular carcinoma (HCC) cells become more sensitive to doxorubicin. Methods: The cytotoxic effects of total RGE or its saponin fraction (RGS) on HCC cells were examined by the lactate dehydrogenase assay in a dose- or time-dependent manner. The effect of RGE or RGS on autophagy was measured by analyzing microtubule-associated protein 1A/1B-light chain (LC)3-II expression and LC3 puncta formation in HCC cells. Late-stage autophagy suppression was tested using tandem-labeled green fluorescent protein (GFP)-monomeric red fluorescent protein (mRFP)-LC3. Results: RGE markedly increased the amount of LC3-II, but green and red puncta in tandem-labeled GFP-mRFP-LC3 remained colocalized over time, indicating that RGE inhibited autophagy at a late stage. Suppression of autophagy through knockdown of key ATG genes increased doxorubicin-induced cell death, suggesting that autophagy induced by doxorubicin has a protective function in HCC. Finally, RGE and RGS markedly sensitized HCC cells, (but not normal liver cells), to doxorubicin-induced cell death. Conclusion: Our data suggest that inhibition of late-stage autophagic flux by RGE is important for its potentiation of doxorubicin-induced cancer cell death. Therapy combining RGE with doxorubicin could serve as an effective strategy in the treatment of HCC.

• Journal of Convergence for Information Technology
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• v.9 no.7
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• pp.135-144
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• 2019

This study was conducted in order to be applied to basic data for enhancing dental hygienists' retention intention. Data analysis was carried out a univariate analysis and a hierarchical regression analysis over 3 stages by using SPSS version 19.0. The dental hygienists' retention intention showed a high outcome in case of the married(p<.001), in the more positive emotion(p<.001), in the lower frequency of emotional expression(p=.010), in the higher happiness index with life satisfaction(p<.001) and in the higher psychological happiness index(p=.012). The dental hygienists' emotion, emotional labor and happiness index need to be recognized as important relevant factors for increasing retention intention.