• The Korean Journal of Mycology
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• v.51 no.4
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• pp.307-311
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• 2023

Powdery mildew anamorphs were collected from Orixa japonica (Rutaceae) in Korea. Based on the morphology and molecular phylogeny derived from the internal transcribed spacer regions and the large subunit gene of the rDNA, the fungus was identified as Erysiphe orixae. This powdery mildew species has been known to be endemic to Japan. This is the first report on E. orixae in Korea.

• Animal Systematics, Evolution and Diversity
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• v.37 no.4
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• pp.322-329
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• 2021

A depressariid moth, Agonopterix issikii Clarke, 1962, feeing on Orixa japonica was newly recorded from Korea. Agonopterix issikii can be characterized by the brownish forewing mottled dark brown and black scales that show a dentate line at the base and three minute blackish dots in triangular at the median, and the basally pale gray hindwing. Agonopterix issikii is similar to A. ocellana Fabricius in external appearance but can be distinguished by the presence of brownish forewing with three blackish triangular dots at the median. The male genitalia of Agonopterix issikii can be characterized by the elliptic gnathos, the triangular socius, the rectangular valva with rather straight cuiller, and the stout phallus with a short basal process and the absence of cornutus. The female genitalia can be characterized by the funnel-shaped ostium bursae, the long ducuts bursae, and the ovate corpus bursae with a rounded patch of signa. To date, 12 species of the genus Agonopterix have been recorded in Korea.

• Korean Journal of Food Science and Technology
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• v.46 no.1
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• pp.39-43
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• 2014

The antimicrobial activity of Orixa japonica Thunb. leaf extract towards 13 microorganism strains was evaluated. Both methanol (MEex) and 70% ethanol extracts showed antimicrobial activity towards Streptococcus mutans, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. MEex showed a higher antimicrobial activity than the 70% ethanol extract. In addition, the dichloromethane fraction (DCMfr) of the MEex also had an antimicrobial effect against the microorganisms examined. The minimum inhibitory concentrations (MICs) towards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 49.22, 24.61, 49.22, and 49.22 mg/mL, respectively. In contrast, the MICs of the DCMfr tpwards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 3.31, 0.21, 1.7, and 1.7 mg/mL, respectively. The MEex antimicrobial activity was not affected by a 3 h exposures to pH in the range of 3-11 or by temperatures were maintained between $80^{\circ}C-100^{\circ}C$ for 6 h. However, the MEex antimicrobial activity decreased at a heat treatment of $121^{\circ}C$ 1 h.

• Korean Journal of Environment and Ecology
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• v.16 no.3
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• pp.296-308
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• 2002

The vascular plants in Mt. Cheongwan, Jangheung-gun, Jeollanam-do, were consisted of 599 taxa; 117 families, 366 genus, 519 species, 76 varieties and 4 forma. From the floristic point of view, Mt. Cheongwan is distributed to both the evergreen trees as Pteris mulfida, Dryopteris bissetiana, Torreya nucifera, Cephlotaxus koreana, Neolitsea sericea, Quercus acuta, Hedera rhombea, Fatsia japonica, Camellia japonica, Eurya japonica, Thea sinensis, Acorus gramineus, Pseudosasa japonica and Sasa borealis, and the deciduous subtropical trees such as Carpinus coreana, Aphanth aspera, Orixa japonica, and Lygodium japonicum And it is worth special mention to be discovered Selaginella rossii, Corylopsis coreana, Stewartia koreana, Asarum sieboldii, Viola orientals, and Drosera royundifolia. The Specific plants which is categorized to the Degree by the Ministry of Environment recorded as 45 taxa; 1 species each for the Degree II, IV and V, 5 species for the Degree III, and 37 species for the Degree I.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.1
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• pp.101-110
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• 2009

The vascular plants in the Mt. Hoemun were listed 541 taxa composed of 113 families, 354 genera, 473 species, 1 subspecies, 64 varieties and 3 forms. Divided into 541 taxa; woody plants were 151 taxa (27.9%) and herbaceous plants were 390 taxa (72.1%). Based on the list of rare plants by the Korea Forest Service, 10 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order: No. 159), Lilium callosum (No. 191), Tricyrtis dilatata (No. 97), Iris ensata var. sponianea (No. 197), Gastrodia elata (No.9), Aristolochia contorta (No. 151), Paeonia obovata (No. 30: Legal protection species), Prunus yedoensis (No. 110 : Planted species), Viola albida (No. 202), Schpolia japonica (No. 208). Based on the list of Korean endemic plants, 15 taxa were recorded; Cephalotaxus koreana, Lilium amabile, Salix purpurea var. japonica, Pseudostellaria multiflora, Aconitum pseudolaeve var. erectum, Filipendula glaberrima, Weigela subsessilis, etc. Based on the list of approved for delivering overseas of plants, 10 taxa were recorded; Carex okamotoi, Lilium distichum, Clematis trichotoma, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha; etc. Specific plant species by floral region were total 4S taxa (8.3% of all 541 taxa of vascular plants); Gastrodia elata, Paeonia obovata in class V, Carex arenicola, Corydalis grandicalyx in class IV, 5 taxa (Spiraea salicifolia, Schpolia japonica, Asperula lasiantha, etc.) in class III, 7 taxa (Lilium distichum, Iris ensata var. spontanea, Caryopteris incana, etc.) in class II and 28 taxa (Camptosorus sibiricus, Orixa japonica, Lonicera praeflorens, etc.) in class I. The naturalized plants in the surveyed sites were 13 families, 38 genera, 43 species, 2 varieties, 45 taxa (Phleum pratense, Medicago sativa, Sonchus asper, etc.) and naturalization rate was 8.3% of all 541 taxa of vascular plants. Ambrosia artemisiifolia var. elatior an ecosystem disturbing wild plants have been increasing. Therefore, the counter plants for continuous control and conservation are needed on the ecosystem of Mt. Hoemun.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.77-84
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• 2012

This study suggested the planting model of Zelkova serrata communities in the areas with the warmth index of both 80~100 and $100{\sim}120^{\circ}C{\cdot}month$. Warmth index was calculated with 449 weather points using inverse distance weighted interpolation method. The planting species were selected by correlation analysis between Z. serrata and each species of four or more frequency among the 36 relev$\acute{e}$ surveyed for this study. The result of this study is summarized as follows : Warmth index of Z. serrata communities was among $74{\sim}118^{\circ}C{\cdot}month$. Results of the correlation analysis between Z. serrata and each species observed that the Z. serrata belongs to the tree layer with warmth index of 80~100 and $100{\sim}120^{\circ}C{\cdot}month$. On the other hand, the species of Carpinus laxiflora, Quercus serrata, Prunus sargentii and Platycarya strobilacea appeared only in the tree layer with warmth index of $80{\sim}100^{\circ}C{\cdot}month$. Z. serrata and Styrax japonica appeared in the subtree layer with the warmth index of 80~100 and $100{\sim}120^{\circ}C{\cdot}month$, while Acer pseudosieboldianum, Lindera erythrocarpa, Acer mono, Quercus serrata, etc. appeared in the subtree layer with the warmth index of $80{\sim}100^{\circ}C{\cdot}month$. Z. serrata, Ligustrum obtusifolium, Lindera obtusiloba, Callicarpa japonica and Zanthoxylum schinifolium all appeared in the shrub layer with the warmth index of 80~100 and $100{\sim}120^{\circ}C{\cdot}month$. Lindera erythrocarpa, Orixa japonica, Staphylea bumalda, Akebia quinata and Sorbus alnifolia appeared in the shrub layer with the warmth index of $80{\sim}100^{\circ}C{\cdot}month$ and Styrax japonica and Stephanandra incisa appeared in the shrub layer with the warmth index of $100{\sim}120^{\circ}C{\cdot}month$, The numbers of each species planted in a $100m^2$ area of the Z. serrata community were suggested as follows : five in tree layer, five in subtree layer and nine in shrub layer. The average area of canopy are suggested to be about $86m^2$ for tree layer, $34m^2$ for subtree layer and $34m^2$ for shrub layer.

• Journal of Life Science
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• v.22 no.6
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• pp.788-793
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• 2012

High concentration of antibiotics has been used to treat the outbreak of edwardsiellosis caused by Edwardsiella tarda in aquaculture. However, not all of the bacteria have been killed with high concentrations of antibiotics treatment by the formation of persister cells with a dormant state. The main objective of this study was to kill persister cell using antibiotics with the addition of natural plant compounds. Antibiotics used in this study consist of 100 mg/ml florfenicol and 100 mg/ml amoxicillin. Ten natural plant compounds with persister cell inhibitor activity to E. coli were obtained from Protein Engineering and Systems Biology Lab. of Sungkyunkwan University. The persister cell inhibition activities of those natural plant compounds were evaluated in test tube. Concentrations of the antibiotics were in the ranges of 25~200 ${\mu}g/ml$. The persister cell formation was observed after 16 hours of culture. Persister cells were killed by antibiotics with natural plant compounds. Among ten natural plant compounds, Gynostemma pentaphyllum, Mallotus japonicus, and Orixa japonica showed persister cell formation inhibition activities. The optimal concentrations of G. pentaphyllum, M. japonicus, and O. japonica for the inhibitor of persister cell formation were 100 ${\mu}g/ml$, 100 ${\mu}g/ml$, and 200 ${\mu}g/ml$, respectively. In vivo study was carried out to evaluate the effect of the antibiotics with natural plant compounds using aquacultural fish, olive flounder, as test animals. G. pentaphyllum, M. japonicus, and O. japonica of 30 ${\mu}g/ml$, 10 ${\mu}g/ml$, and 10 ${\mu}g/ml$ with antibiotics reduced cumulative mortalities, showing the effectiveness of persister cell inhibition.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.133-144
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• 2007

The vascular plants at the Wolmyung Park was listed 479 taxa: 105 families, 317 genera, 417 species, 1 subspecies, 55 varieties and 6 forms. 479 taxa listed consists of 291 taxa of edible plants (60.8%), 335 taxa of medicinal plants (69.9%), 234 taxa of ornamental plants (48.9%) and 243 taxa of the others (50.7%). Based on the list of the rare plants by the Forest Research Institute, 4 taxa were recorded in the investigated areas; Aristolochia contorta (Preservation priority order, No. 151), Magnolia kobus (No. 187), Prunus yedoensis (No. 110), Berchemia racemesa (No. 67) and based on the list of Korean endemic plants, 9 taxa were recorded; Cephalotaxus koreana, Carex okamotoi, Populus tomentiglandulosa, Prunus yedoensis, Forsythia koreana, Asperula lasiantha, Weigela subsessilis, Adenophora triphylla var. hirsuta, Aster koraiensis. Specific plant species by floral region were total 31 taxa; Prunus yedoensis, Berchemia racemosa in class V, Magnolia kobus, Wistaria floribunda in class IV, 4 taxa (Spiraea salicifolia, Acer palmatum, Asperula lasiantha, etc.) in class III, Caryopteris incana in class II, 22 taxa (Clematis patens, Orixa japonica, Euscaphis japonica, Grewia biloba var. parviflora, etc.) in class I. The naturalized plants in this site were 14 families, 35 genera, 44 species, 2 varieties, 46 taxa and naturalization rate was 9.6% of all 479 taxa vascular plants.

• Korean Journal of Environment and Ecology
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• v.21 no.1
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• pp.38-46
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• 2007

The vascular plants of the Moaksan provincial park area were listed as 615 taxa: 120 families, 383 genera, 542 species, 1 subspecies, 69 varieties and 3 forms. Based on the list of Korean endemic plants, 6 taxa were recorded: Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Salix purpurea var. japonica, Weigela subsessilis, Aster koraiensis and based on the list of the rare plants by the Forest Research Institute, 5 taxa were recorded in the studied areas: Lilium distichum (Preservation priority order; No. 159), Tricyrtis dilatata (No. 97), Iris ensata var. spontanea (No. 197), Aristolochia contorta (No.151), Viola albida (No. 202). Specific plant species by floral region were a total of 48 taxa; Prunus yedoensis in Class V, 2 taxa, as Carex arenicola, Wistaria floribunda in Class IV, 4 taxa, Spiraea salicifolia, Thea sinensis, Asperula lasiantha, etc. in Class III, 8 taxa, as Lilium distichum, Iris ensata var. spontanea, Acer triflorum, etc. in Class II, 33 taxa, as Camptosorus sibiricus, Orixa japonica, Lonicera praeflorens, etc. in Class I. The naturalized plants in this site were 16 families, 40 genera, 48 species, 2 varieties, 50 taxa and the naturalization rate was 8.1% of all 615 taxa vascular plants. As a conservation measure, there needs to be controls on the imprudent development and excessive visitors and a detailed plan like nature the rest of the year.