• BMB Reports
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• 제34권5호
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• pp.472-477
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• 2001

Ornithine decarboxylase (ODC, EC 4.1.1.17) is the first and key enzyme in eukaryotic polyamine biosynthesis. The cDNA encoding ornithine decarboxylase from Nicotiana glutinosa was cloned ($GeBank^{TM}$ AF 323910) and expressed in E. coli. Site directed mutagenesis were performed on several highly conserved cysteine residues. Among the mutants, C115A showed significant changes in the kinetic properties. The $K_m$ value of the C115A mutant was $1790\;{\mu}M$, which was 3-fold higher than that of the wild-type ODC. There was a dramatic decrease in the $k_{cat}$, values of the C115A mutant, compared to that of the wild-type ODC, which had a $k_{cat}$ value of $77.75\;s^{-1}$. C115A caused a shift in the optimal pH from 8.0 to 8.4. Considering these results, we suggest that cys-115 is involved in the catalytic activity of N. glutinosa ODC.

• Journal of Advanced Marine Engineering and Technology
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• 제38권10호
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• pp.1212-1216
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• 2014

This study aims to find out the best anode location for buried pipelines. Numerical simulation program known as CATPRO (Elsyca, Belgium) were used for confirming the best location of anodes and the effects of impressed current cathodic protection system. Applied conditions for numerical simulation were similar to on-site environmental conditions for optimal application of cathodic protection system. Used criterion of cathodic protection was NACE SP 0169, which describes that minimum requirement for cathodic protection is -850mV vs. CSE. Various layouts for anodes' installation were applied, which were distance between anodes, anode installation location, and applied current. The areas where cathodic protection potential was lower than -850mV vs. CSE was limited up to 50m from anode installation locations. It was founded numerical analysis obtain cost-effective and efficient cathodic protection methods before design and application the impressed cathodic protection system to on-site environment.

• Journal of the Korean Operations Research and Management Science Society
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• 제30권1호
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• pp.187-198
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• 2005

The structure of a Web site can prevent the search robots or crawling agents from confusion in the midst of huge forest of the Web pages. We formalize the view on the World Wide Web and generalize it as a hierarchy of Web objects such as the Web as a set of Web sites, and a Web site as a directed graph with Web nodes and Web edges. Our approach results in the optimal hierarchical structure that can maximize the weight, tf-idf (term frequency and inverse document frequency), that is one of the most widely accepted content centric measures in the information retrieval community, so that the measure can be used to embody the semantics of search query. The experimental results represent that the optimization model is an effective alternative in the dynamically changing Web environment by replacing conventional heuristic approaches.

• Journal of Communications and Networks
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• 제16권4호
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• pp.430-435
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• 2014

The performance of a distributed database system depends particularly on the site-allocation of the fragments. Queries access different fragments among the sites, and an originating site exists for each query. A data allocation algorithm should distribute the fragments to minimize the transfer and settlement costs of executing the query plans. The primary cost for a data allocation algorithm is the cost of the data transmission across the network. The data allocation problem in a distributed database is NP-complete, and scalable evolutionary algorithms were developed to minimize the execution costs of the query plans. In this paper, quadratic assignment problem heuristics were designed and implemented for the data allocation problem. The proposed algorithms find near-optimal solutions for the data allocation problem. In addition to the fast ant colony, robust tabu search, and genetic algorithm solutions to this problem, we propose a fast and scalable hybrid genetic multi-start tabu search algorithm that outperforms the other well-known heuristics in terms of execution time and solution quality.

• Proceedings of the KSR Conference
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• 한국철도학회 2005년도 추계학술대회 논문집
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• pp.424-442
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• 2005

In this paper a design of a large shield Tunnel through weathered soil and weathered rock in 703 section of seoul subway line No.7 extension project is presented. The geological investigation results show that the projecet region consists mostly of weathered soil with some local weathered rock in the tunnel excavation level. A EPB shield TBM is selected as a optimal excavation machine for the large shield Tunnel considering the geological and site conditions. Also, the shield machine head and cutter for the large shield tunnel type are designed considering site geological conditions and average advance rate in similar projects.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 한국전기전자재료학회 2000년도 하계학술대회 논문집
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• pp.198-203
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• 2000

This paper investigated the effects of doping elements on the Bi-Sr-Ca-Cu-O ceramics. The doping elements can be classified into four groups depending on their superconducting characteristics in the Bi-Sr-Ca-Cu-O structure. The first group of doping elements(Co, Fe, Ni and Zn) substitute into the copper site and can reduce the critical temperatures of the 2223 and 2212 phases. The second group of doping elements(Y and La) substitute into the Ca site and cause the disappearance of the 2223 phase and increase the critical temperatures in the 2212 phase. The third group of doping elements(P and K) have a tendency to decompose the superconducting phase and reduce the optimal sintering temperature. The fourth group of doping elements(B, Si, Sn and Ba) almost unaffected the superconductivity of the 2223 and 2212 phase.

• Electrical & Electronic Materials
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• 제9권1호
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• pp.1-8
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• 1996

We investigated the effects of doping elements on the Bi-Sr-Ca-Cu-O ceramics. The doping elements can be classified into four groups depending on their supeconducting characteristics in the Bi-Sr-Ca-Cu-O structure. The first group of doping elements(Co, Fe, Ni and Zn) substitute into the copper site and can reduce the critical temperatures of the 2223 and 2212 phases. The second group of doping elements(Y and La) substitute into the Ca site and cause the disappearance of the 2223 phase and increase the critical temperatures in the 2212 phase. The third group of doping elements(P and K) have a tendency to decompose the superconducting phase and reduce the optimal sintering temperature. The fourth group of doping elements(B, Si, Sn and Ba) almost unaffected the superconductivity of the 2223 and 2212 phase.

• Proceedings of the Korean Institute of Building Construction Conference
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• 한국건축시공학회 2023년도 가을학술발표대회논문집
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• pp.129-130
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• 2023

This study proposes rainwater infiltration retention blocks as a solution to the flooding problems caused by recent climate change and developed a flood prediction simulation program to select the optimal site for installing rainwater infiltration retention blocks that can minimize damage from floods. By applying the existing 2D flood analysis model G2D and adding a reservoir function, the volume of water before and after installation can be determined through simulation results.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 한국농공학회 2001년도 학술발표회 발표논문집
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• pp.319-323
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• 2001

This study was conducted to derive the regional design rainfall by the regional frequency analysis based on the regionalization of the precipitation. Using the L-moment ratios and Kolmogorov-Smirnov test, the underlying regional probability distribution was identified to be the Generalized extreme value distribution among apt]lied distributions. regional and at-site parameters of the Generalized extreme value distribution were estimated by the method of L-moment. The regional and at-site analysis for the design rainfall were tested by Monte Carlo simulation. Relative root-mean-square error(RRMSE), relative bias(RBIAS) and relative reduction(RR) in RRMSE were computed and compared with those resulting from at-site Monte Carlo simulation. All show that the regional analysis procedure can substantially reduce the RRMSE, RBIAS and RR in RRMSE in the prediction of design rainfall. Consequently, optimal design rainfalls following the regions and consecutive durations were derived by the regional frequency analysis.

• Smart Structures and Systems
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• 제25권5호
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• pp.631-641
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• 2020

To meet the urgent requirements of safety surveillance from civil engineering management authorities, this study proposes a refined and efficient approach to generate full-field high-resolution panorama of construction sites using camera-amounted UAV (Unmanned Aerial Vehicle). GPS (Global Position System) information extraction for pre-registration, feature points filtering for efficient registration and optimal seaming line seeking for fusion are performed in sequence to form the full-field panorama generation framework. Advantages of the proposed method are as follows. First, GPS information can sort images for pre-registration, avoiding inefficient repeated pairwise calculations and matching. Second, the feature points are filtered according to the characteristics of the construction site images to reduce the amount of calculation. The proposed framework is validated on a road construction site and results demonstrate that it can generate an accurate and high-quality full-site panorama for the safety supervision in a much efficient manner.