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Neuropretective effect of Kupunggibodan, Gamisamul-tang and Whangryunhaedok-tang on the ischemia-induced learning and memory deficits by MCAO in the rats (중풍 한방처방전의 효능비교 연구 ; 황련해독탕, 거풍지보단, 가미사물탕이 국소 전뇌허혈에 의한 학습과 기억에 미치는 효과)

  • Lee Bom-Bi;Chung Jin-Yong;Kim Sun-Yeou;Kim Ho-Cheol;Kwon Youn-Jun;Hahm Dae-Hyun;Lee Hae-Jeong;Shim In-Sup
    • Korean Journal of Acupuncture
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    • v.19 no.2
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    • pp.63-78
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    • 2002
  • Kupunggibodan(KU), Gamisamul-tang(GA) and Whangryunhaedok-tang(WH) are clinically the most popular prescriptions as an herbal medicine in the treatment of ischemia. In order to compare and evaluate their protective effects on the ischema-induced cognitive deficits by middle cerebral artery occlusion (MCAO), we examined its ability to improve ischemia-induced cell loss and impairements of learning and memory in the Morris water maze and eight-arm radial arm maze. Focal cerebral ischemia produced a marked cell loss, decrease in acetylcholinesterase(AchE) reactivity in the hippocampus, and learning and memory deficits in two behavioral tasks. Pretreatment with WH (100 mg/kg, p.o.) produced a substantial increase in acquisition in the Morris water maze. Pretreatment with KU increased the perfomance of the resention test in the Morris water maze. WH, KU and GA caused a significant improvement in choice accuracy in radial arm maze test. WH was superior to KU and GA in perfomance of the radial arm maze test. Consistent with behavioral data, staining with cresyl violet showed that pretreatments with WH, but not KU and GA significantly recovered the ischemia-induced cell loss in the hippcampal CA1 area. In addition, pretreatments with WH and KU recovered the ischemia-induced reduction of AchE reactivity in the hippocampal CA1 area. These results demonstrated that KU, GA and WH have protective effects against ischimea-induced learning and memory impairments and that the efficacy was the order of WH>KU>GA in tratment of ischemia induced memory deficits. The present studies provide an evidence of KU, GA and WH as putative treatment of vascular dementia. Supported by a fund from the Ministry of Health and Welfare(HMP-00-OO-04-0004), and the Brain Korea 21 Project from Korean Ministry of Education, Korea.

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Factors Regulating the Nitrogen Fixation Activity and Growth of Anabaena variabilis ATCC 29413 (Anabaena variabilis ATCC 29413 의 생장과 질소고정활성의 조절요인)

  • 송승달;한동훈
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.391-396
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    • 1992
  • Anabaena variabilis A TCC 29413. a photoautotrophic and nitrogen fixing cyanobacteria. was investigated on the environmental factors regulating the growth and nitrogen lixation activity. A good growth of cyanobacteria] cells was observed due to nitrogen t1xation by the heterocyst differentiation in nitrogen free Allen and Arnon (]/8) medium. The nitrogenase activity was appeared to be in proportion to the cell growth lor 6 days then drastically decreased in the later growth period when the nitraTe was accumulated to high level in the culture to cause the inhibition. The optima] conditions lilr the cell growth and nitrogenase activity of A. varillbili.l were anaerobic. IO.OO0 lux. $30^{\circ}C$ and pH 8 with the nitrogen Cree minimal medium. The activity was significantly inhihited by the low concentrations of ammonium and nitrate. but was stimulated b) the ]ow Ieve] of phosphate and carbonate sources. The treatments of several toxic heavy metals showed strong inhibition of the cell growth and nitrogenase activity by O.3~10 ppm in the order of $Hg^{2+}$ > $Cd^{2+}$ > $Co^{2+}$ > $Zn^{2+}$ > $Ph^{2+}$, and the concentrations for 50% inhibition of the maximum activity were 0.41. 0.47. 0.5 L 0.66 and 8.1 ppm. respectively. The addition of carbohydrates (0.5~ 1.0%) in the dark condition stimulated the growth and activity in the order of sucrose > fructose > glucose.

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Effect of Micelles on the Reaction of RuBPCase in Maize Leaf (옥수수 잎의 RuBPCase 반응에 미치는 미셀들의 영향)

  • 김의락;김현묵
    • KSBB Journal
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    • v.9 no.3
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    • pp.325-331
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    • 1994
  • PGA is formed in a route of CO2 fixation of RuBP catalyzed by RuBPCase, followed by reduction of the PGA by NADH to GAP This reduction is enhanced in an anionic micellar solution(SDS), in which NADH is distributed in the aqueous and the micellar pseudophases in a given ratio. This micellar bounded NADH reacts to PGA, and in higher micellar concentration than $1.25{\times}10^{-2}M$, most of NADH is oxidized to NAD+ by PGA. On the other hand, in the solutions of the positive ionic(CTABr), zwitter ionic(Chaps) and nonionic (Brij and Triton X-100) micelles, the reactions are also enhanced and the concentrations of NADH reach minima with micellar concentrations. Such minima are typical of micellar catalyzed bimolecular reactions, and the fall in concentrations of the reductant followed by a gradual increase is charataristic of reactions of hydrophobic substrates: that is, the reductions of PGA by NADH are sharply enhanced in a range of the lower micellar concentrations, and NADH amounts in ca. $1.25-2.50{\times}10^{-3}M$ micellar solutions are reached to minima, followed by gradual increases of the reductant concentration.

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Survey on Satisfaction and Symptom Improvement of Korean Medicine Treatment in 122 Cases by Traffic Accident (교통사고 환자 122례에 대한 한방치료 만족도 및 호전도 조사)

  • Kim, Hye Ryeon;Kim, Seon Hye;Lee, Yeon Sun;Park, Seo Hyun;Sung, Won Suk;Cho, Hyun Seok;Keum, Dong Ho;Kim, Kap Sung;Kim, Eun Jung
    • The Journal of Korean Medicine
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    • v.39 no.3
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    • pp.61-72
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    • 2018
  • Objectives: This study conducted a survey research to characterize traffic accident(TA) patients seeking Korean medicine treatment and to analyze the symptom improvement and satisfaction scores. Methods: A survey was conducted in 122 outpatients, who visited OO University Korean Hospital due to TA-associated symptoms from November, 2017 to May, 2018. The questionnaire included information on patient demographic characteristics, accident circumstance details, pain levels, reason for treatment selection, treatment methods, treatment purpose, symptom improvement and satisfaction. All statistical analyses were performed using Windows SPSS version 20.0 Results: The characteristics of traffic accident patients using Korean medical institution were usually accidents that occurred while driving slowly, and minor injuries like a sprain. The most important consideration in choosing Korean medical institution was its past experience. The biggest reason for switching treatment from Western medicine to Korean medicine was for diverse treatments. Satisfaction with Korean medicine was measured between very satisfaction and satisfaction. Patients showed the highest satisfaction with acupuncture, followed by pharmacopuncture and chuna manual therapy. Most treatments were measured between very satisfaction and satisfaction. After treatment, 95.90 percent of the patients said they would recommend Korean treatment. Conclusions: Although this study has limitations as research in survey format, we intended to analyze determining factors for the use of Korean medicine treatment through satisfaction, symptom improvement, and Numeric rating scale (NRS) change.

The Influence of Functional Electric Stimulus Treatment and Neurological Development Treatment on the lower limbs' Spasticity of the Patients with Hemiplegia (기능적전기자극 치료와 신경발달치료가 편마비 환자의 하지 경직에 미치는영향)

  • Choi Hyun-ja;Oh Jung-lim;Park Rae-joon
    • The Journal of Korean Physical Therapy
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    • v.15 no.3
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    • pp.388-411
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    • 2003
  • The purpose of this study has been conducted to reduce the lower limbs' spasticity of the patients with hemiplegia caused by cerebral stroke of apoplexy and find differences about spasticity effects among each group. The objects of this study covered 24 patients with hemiplgia who are either in the oo hospital in Daegu or under treatment from home to hospital. The objects fall into three groups which are a group of neurological development treatment, a group of functional stimulus treatment and a group of neurological development treatment and functional stimulus treatment. The result of this study were as follows : 1) The neurological development treatment has been found to reduce the lower limbs' spasticity of patients with hemiplegia caused by cerebral stroke of apoplexy and compared to before-treatment, the MAS value of spasticity has been shown to be statistically meaningful ,and gradually over the period of between 4 weeks and 8 weeks(P <.05). 2) The functional electric stimulus treatment has been shown to reduce the lower limb's spasticity of patients with hemiplegia caused by cerebral stroke of apoplexy and compared to before-treatment, the MAS value of spasticity was statistically meaningful and compared to 4 weeks, even at the time of 8 weeks, the MAS value of spasticity have shown statistical meaningness. (P <.05) 3) When neurological development treatment and functional electric stimulus treatment was applied at the same time, the lower limbs' spasticity of patients with hemiplegia was reduced meaningfully(P <.05). Compared to before-treatment at the time of 4 weeks, the MAS value of spasticity was statistically meaningful and compared to 4 weeks at the time of 8 weeks the MAS value of spasticity was also statistically meaningful(P <.05) 4) In the case of time-based MAS value of each group, functional electric stimulus treatment reduced the spasticity more meaningfully than neurological development treatment, and the group of same application of functional electric stimulus treatment and neurological development treatment showed better statistical meaningness than functional electric stimulus treatment alone(P <.05) and finally the group of same application of neurological development treatment and functional electric stimulus treatment showed more meaningful difference than neurological development treatment alone(P <.05)

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Effects of Thoracolumbosacral Orthosis on Cobb angle at The Idiopathic Scoliosis (흉요천추 보조기착용이 특발성 척추 측만증 환자의 Cobb 각도 개성에 미치는 효과)

  • Song Jun-Chan;Lee Hyun-kee;Chang In-Su;Park Rae-Joon
    • The Journal of Korean Physical Therapy
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    • v.14 no.4
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    • pp.274-307
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    • 2002
  • The TLSO was customized for this study in OO ortho-prosthesis institute from Jan. 1th., 2000 to Dec. 31th., 2000 and in order to measure effects of TLSO. 20 females in a growth period applicable to medical care took part in this study, they were on accidental spine scoliosis (From $15^{\circ}to35^{\circ}$). They were consisted of Group I(10 chest-bend) and Group II (10 dual-bend). The results were follows: 1. It showed the average difference in height by 1.37cm, 3.14cm in comparison between before and after TLSO, before and after one year and they were also statistically available(p < .05). 2. It showed the average difference in Cobb angle of a chest and waist by ($-10.95^{\circ},-8.50^{\circ}$), between before and right after TLSO. The results means that the Cobb angle of the chest and weist at right after TLSO was largely decreased, and it was also statistically available(p < .05). 3. It showed the difference in Cobb angle of the chest waist by $-9.50^{\circ},-7.35^{\circ} $ between before and one year after TLSO. It means that the Cobb angle of the chest and waist at the one year after TLSO was largely decreased, and it was also statistically available(p < .05). 4. It showed the difference in Cobb angle of the chest and waist by $2.34^{\circ},2.15^{\circ} $ between the right after and the one year after. TLSO, but the change of Cobb angle of the chest was regularly constant by a little increased, and it was also statistically available(p < .05). 5. In the measurement of the change of Cobb angle of the chest and waist according to the taking time on TLSO, it showed the slightest change in 10 people on TLSO for 23 hours by $13.30^{\circ},11.20^{\circ}$ , the change in 6 people on TLSO for 16 hours by $14.75^{\circ},12.67^{\circ}$, the change in 4 people on TLSO for 8 hours by $16.83^{\circ},14.00^{\circ} $ in this order. It means that the longer time on TLSO was to be the smaller the Cobb angle of the chest and waist, but it was not statistically available.

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Electrical Characteristics of PECVD $Ta_2O_5$ Dielectic Thin Films on HSG and Rugged Polysilicon Electrodes (입체표면 폴리실리콘 전극에서 PECVD $Ta_2O_5$ 유전박막의 전기적 특성)

  • Cho, Yong-Beom;Lee, Kyung-Woo;Chun, Hui-Gon;Cho, Tong-Yul;Kim, Sun-Oo;Kim, Hyeong-Joon;Koo, Kyung-Wan;Kim, Dong-Won
    • Journal of the Korean Vacuum Society
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    • v.2 no.2
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    • pp.246-254
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    • 1993
  • In order to increase the capacitance of storage electrode in the DRAM capacitor, two approaches were performed. First, hemispherical and rugged poly silicon films were made by LPCVD to increase the effective surface area of storage electrode. The even surface morphology of conventional poly silicon electrode was changed into the uneven surface of hemispherical of rugged poly silicon films. Second, PECVD $Ta_2O_5$ dielectric films were deposited and thermally treated to study the dielectrical characteristics of $Ta_2O_5$ film on each electrode. MIS capacitors with $Ta_2O_5$ films were electrically characterized by I-V, C-V and TDDB measurements. As a result, the capacitance of the electrode with uneven surface were increased by a factor of 1.2~1.5 and leakage current was increased compared with those of even surface. TDDB result indicates that the electrode with uneven surface has dielectrically more degraded than that of even surface. These results can be helpful as a basic research to develop new generation DRAM capacitors with $Ta_2O_5$ films.

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The Effects of ETC-based Group Art Therapy : Focusing on the Parental Anxiety of Elementary School Students' Mothers (ETC 기반 집단미술치료의 효과성 연구: 초등학생 어머니의 양육불안 감소를 중심으로)

  • Park, Sang-Soon;Rim, Sung-Ryun
    • The Journal of the Korea Contents Association
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    • v.21 no.4
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    • pp.303-316
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    • 2021
  • This study is aimed at verifying the effects of an ETC(Expressive Therapies Continuum)-based group art therapy program on reducing parental anxiety in first-grade elementary school students' mothers. An ETC program was composed based on the therapeutic factors of the ETC components. Seven mothers of first-grade students located in city A of Gyeonggi-do were selected as the subjects of the study. These mothers underwent 11 sessions of ETC-based group art therapy from April 18 to May 24, 2019, 1 to 2 times a week for 70 to 90 minutes. In order to verify the effectiveness of this research program, scores from pre- and post-parental anxiety scales were analyzed using SPSS 24.0 program. Results demonstrated that first, mothers' parental anxiety was significantly reduced after the ETC program participation. Second, changes in ETC component use throughout the program positively influenced reductions in parental anxiety. In conclusion, each participant experienced the opportunity to self-explore and self-understand at all levels of ETC with their preferred art medium, thereby lowering parenting anxiety. It is meaningful that through the ETC-based art therapy program, the client can be self-aware of his or her problem, and the client can decide the direction that is beneficial to them through voluntary media selection. In addition, it is meaningful that the ETC group art therapy is able to perform activities tailored to each individual.

Effects of Photoperiod on Oviposition and Emergence of Egg Parasitoid, Trichogramma dendrolimi Mastumura (Hymenoptera, Trichogrammatidae) (광조건이 송충알벌(벌목, 알벌과)의 산란과 우화에 미치는 영향)

  • 박영규;이해풍;이기상;한만위;이정운
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.93-99
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    • 1999
  • We investigated the effect of constant photoperiod (16L : 8D, 12L : 12D, 8L : 16D), on the oviposition, parasitism, and emergence of Trichogramma dendrolimi. Also during 16L : 8D condition circadian, oviposition and emergence of this species on artificial eggs were checked every 2hrs. When twelve eggs of Antheraea pern~i and artificial eggs were provided, the wasps had the highest rate of oviposition in the first day, and decreased dramatically there after. In these different photoperiod, the total oviposition numbers per A. pernyi eggs by the wasp were 161.8 in 16L : 8D, 145.8 in 121 : 12D and 128.5 in 8L : 16D respectively in the first day. On the other hand for the artificial egg in 16L : 8D photoperiod, the total oviposition number per egg was 106.8 in the first day, 26.6 in the 2nd day and 21.3 in the 3rd day. The rate of emergence from A. pernyi eggs was not different depend on wasp's day age, however in the artificial eggs, after the first day's 84.7% decreased considerably. The emergence rate from artificial eggs was the highest comparatively between 04:OO-0600 hours.

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An Analysis on the Health Education Content Suggested in the 7th Curriculum of Elementary School Education (제7차 초등학교 교육과정에 제시된 보건교육 내용 분석)

  • Kim, Gha-Ok;Park, Young-Soo
    • The Journal of Korean Society for School & Community Health Education
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    • v.2 no.2
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    • pp.39-55
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    • 2001
  • The purpose of this study was to provide the necessary fundamental data in composing a systematic text content of the public health by analyzing each text, domain, and teaching contents suggested in the textbooks as well as teachers' guides of the 7th elementary school education curriculum, while the study subjects were as follows. 1. The health education content suggested in the 7th physical education curriculum were analyzed and examined. 2. The health teaching content of each textbook in the 7th elementary school curriculum was to be analyzed and examined. In order to resolve the above research issues, the physical, spiritual, and social domain along with the (1) Proper living habit, (2) Health and nutrition, (3) Sex education, (4) Prevention of the sense-organic diseases, (5) Cleanliness of food, (6) Oral hygiene, (7) Individual health and public health, (8) Safety in living, (9) Abuse and usage of medication, educational content suggested in the 7tand (10) Environment pollution focused around the health of the elementary school education curriculum was analyzed and its outcome was as below First, compared with the 6th elementary school education curriculum, the health content suggested in the 7th elementary school education curriculum was decreased. Second, although each grade's teaching content of the health domain in the physical education was considered in its structure following after the according systems, they were preponderant in partial subjects such as the safety in living, nutrition, proper living habit, sport, and health in sport. oo. Third, the health education content was organized in 4 units such as the physical growth and development, prevention of diseases, safe living, and leisure living(leisure, spiritual health, and etc.) for the 3rd and 4th grade. Then, as for 5th and 6th grade, it was organized in 3 units such as the understanding the human body, prevention of disease, and leisure and safe living. Fourth, in the physical educational health domain, a strong point was constructed within the physical, spiritual, and social areas of the elementary school physical education. Fifth, the number of the public health education contents directly related with the health education was 43 as with 25 indirect contents. Sixth, each grade's domain unit structure of the public health content was heavy upon the physical and social area throughout every grade while in opposite, the spiritual domain' s unit structure was weak. In according to each grade, the physical domain was stressed in 4, 5, and 6 grades while the social domain was stressed in 1, 5, and 6 grades.

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