• 생명과학회지
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• 제31권10호
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• pp.873-884
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• 2021

본 연구에서는 다양한 인체 암포주를 대상으로 NSAID의 항암 효과를 증강시키는 artesunate (ART)의 역할과 이에 대한 분자적 기전을 연구하였다. 다양한 타입의 암세포주를 대상으로 암세포 성장 억제 활성을 조사한 결과, ART는 NSAID인 celecoxib (CCB) 또는 dimethyl-CCB (DMC)와의 병용 효과를 나타내었다. ART 처리로 ATF4/CHOP의 발현 증강과 함께 오토파지 유도 표식인 p62 감소의 결과로서, ATF4/CHOP 경로가 ART의 오토파지 유도 활성에 관여할 것으로 예상되었으며, ART의 오토파지 활성과 관련하여 NRF2 및 암 줄기 세포 관련 단백질인 CD44, CD133, ALDH1, Oct4, mutated p53 (mutp53) 및 c-Myc의 발현이 감소되었다. 또한 DMC 단독처리 보다 ART와 DMC의 병용으로 ATF4/CHOP의 발현 증강과 p62의 감소가 더욱 촉진되고, NRF2 및 암 줄기 세포 관련 단백질 발현 감소도 현저히 촉진되면서 궁극적으로 PARP 활성화에 의해 apoptosis가 유도됨을 알 수 있었다. 이러한 결과는 ART/DMC 병용 처리가 각 물질 단독 처리보다 암세포의 성장 억제 및 apoptosis 유도에 더욱 효과적이고, ART 및 DMC 의 오토파지 유도 활성은 암 줄기 세포 관련 단백질의 분해를 촉진함으로써, 암 줄기 세포가 제거될 수 있음을 시사하였다. 이와 같이 ART는 NSAID 뿐만 아니라 imatinib의 항암 효과를 증강시키는 활성으로, chemosensitizer로서 중요한 후보 물질이 될 수 있음을 밝혔다.

• 한국초지조사료학회지
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• 제33권2호
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• pp.117-122
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• 2013

본 시험은 2010년부터 2012년까지 경북 성주에서 2모작 작부체계에 알맞은 다수성이며 조숙성인 콤먼베치의 품종을 선발 할 목적으로 헤어리베치 Cold green, 콤먼베치 Maxivesa, Wild common, Rasina, Morava 및 Blanchefleur의 생육특성, 건물수량 및 사초품질을 평가하였다. 시험설계는 품종을 처리로한 6처리 3반복의 난괴법으로 배치하였다. 파종은 2010년 10월 11일과 2011년 10월 3일에 하였으며 수확은 2011년 5월 4일과 2012년 5월 10일에 실시하였다. 가을의 초형은 Wild common은 부복형이었으며 다른 품종들은 반직립형이었다. 유식물의 활력은 다른 품종들에 비해 Wild common이 다소 낮았다. 내한성은 Cold green과 Wild common이 우수했으나 다른 콤먼베치 품종들은 낮았다. 개화기는 Cold green이 4월 22일로 가장 빨랐으며 Wild common은 4월 26일 Rasina와 Blanchefleur가 4월 28일이었으며 Maxivesa가 5월 17일이었다. 1년차 시험에서 건물수량은 Cold green은 다른 5품종보다 수량이 높았으며 (p<0.05), Wild common은 나머지 4품종보다 많았으며 (p<0.05), Maxivesa와 Blanchefleur는 Rasina와 Morava보다 높았다 (p<0.05). 2년차 시험에서도 건물수량은 Cold green은 다른 5품종 보다 높았으며 (p<0.05), Wild common은 다른 4품종보다 높았다 (p<0.05). 일반적으로 베치의 CP 함량은 높았고 섬유소함량은 낮았다. 가을 파종 콤먼베치의 품종은 개화기가 빠르며 내한성이 강한 Wild common이 좋을 것으로 생각된다.

• 한국초지조사료학회지
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• 제13권3호
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• pp.213-220
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• 1993

Tall fesuce 9 개 품종을 공시하여 가을철 예취시기를 달리했을 때 l 번초의 수량구성요소와 개체중에 미치는 영향을 조사하여 가을철 최종예취시기를 추정하고 이 지역의 환경조건에 적응성이 높은 품종 을 선발하려고 하였다. l. 가을철 예취시기에 따라서 l 번초의 개체중에 관여하는 수량구성요소의 차이가 인정되었는데 $C_1-C_3$의 l 번초 개체중은 출수관련형질들과 유의한 정상관이 인정되었으나 $C_4$의 1 번초 개체중에서는 유의한 상관이 인정되지 않았다. 2. $C_1C_3$에서는 출수경수가 많고 출수경중과 l 경중이 무거웠던 Forager의 개체중이 많았으나 $C_4$에서는 개체당 경수가 많았던 Barcel의 개체중이 많았다. 3. 대관령지역의 영년초지에서 가을철 최종예취시기의 한계는 $C_1$시기 (9월 30일) 로 추정되었으며 최종 예취시기의 위험기는 $C_2$시기 (10월 14일)의 전후로 추정되었다. 4. 1번초의 개체중은 가을철 예취시기와 유의한 부(負)의 상관을 나타내었다.

• Clinical and Experimental Reproductive Medicine
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• 제29권2호
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• pp.129-138
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• 2002

Objective: This study was to compare the characteristics between parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. Materials and Methods: Mouse oocytes were recovered from superovulated 4 wks hybrid F1 (C57BL/6xCBA/N) female mice. For parthenogenetic activation, oocytes were treated with 7% ethanol for 5 min and $5{\mu}g$/ml cytochalasin-B for 4 h. For IVF, oocytes were inseminated with epididymal sperm of hybrid F1 male mice ($1{times}10^6/ml$). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count of blastocysts in those two groups was taken by differential labelling using propidium iodide (red) and bisbenzimide (blue). To establish ES cells, b1astocysts in IVF and parthenogenetic groups were treated by immunosurgery and recovered inner cell mass (ICM) cells were cultured in LIF added ES culture medium. To identify ES cells, the surface markers alkaline phosphatase, SSEA-1, 3,4 and Oct4 staining were examined in rep1ated ICM colonies. Chromosome numbers in P-mES and mES were checked. Also, in vitro differentiation potential of P-mES and mES was examined. Results: Although the cleavage rate (${\geq}$2-cell) was not different between IVF (76.3%) and parthenogenetic group (67.0%), in vitro development rate was significantly low in parthenogenetic group (24.0%) than IVF group (68.4%) (p<0.05). Cell number count of ICM and total cell in parthenogenetic b1astocysts ($9.6{\pm}3.1,\;35.1{\pm}5.2$) were signficantly lower than those of IVF blastocysts ($19.5{\pm}4.7,\;63.2{\pm}13.0$) (p<0.05). Through the serial treatment procedure such as immunosurgery, plating of ICM and colony formation, two ICM colonies in IVF group (mES, 10.0%) and three ICM colonies (P-mES, 42.9%) in parthenogenetic group were able to culture for extended duration (25 and 20 passages, respectively). Using surface markers, alkaline phosphatase, SSEA-l and Oct4 in P-mES and mES colony were positively stained. The number of chromosome was normal in ES colony from two groups. Also, in vitro neural and cardiac cell differentiation derived from mES or P-mES cells was confirmed. Conclusion: This study suggested that P-mES cells can be successfully established and that those cell lines have similar characteristics to mES cells.

• Clinical and Experimental Reproductive Medicine
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• 제32권2호
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• pp.133-147
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• 2005

Objectives: This study was carried out to establish human embryonic stem cells derived from frozen-thawed embryos using mouse embryonic fibroblasts (mEFs), human fetal skin and muscle fibroblasts as feeder cells, and to identify the characteristic of embryonic stem cells. Methods: When primary mEFs, human fetal skin and muscle fibroblasts were prepared, passaging on 4 days from replating could have effective trypsinization and clear feeder layers. Eight of 23 frozenthawed 4~8 cell stage embryos donated from consenting couples developed to blastocysts. Inner cell mass (ICM) was isolated by immunosurgery. ICM was co-cultured on mEFs, human fetal skin or muscle fibroblasts. The ICM colonies grown on mEFs, human fetal skin or muscle fibroblasts were tested the expression of stage specific embryonic antigen-3, -4 (SSEA-3, -4), octamer binding transcription factor-4 mRNA (Oct-4) and alkaline phosphatase surface marker. Results: We obtained 1 ICM colony from 2 ICM co-cultured on mEFs as feeder cells and did not obtain any ICM colony from 6 ICM clumps co-cultured on human fetal skin or muscle fibroblasts. The colony formed on mEFs could be passaged 30 times every 5 days with sustaining undifferentiated colony appearance. When the colonies cultured on mEFs were grown on human fetal skin or muscle fibroblasts, the colonies could be passaged 15 times every 9 days with sustaining undifferentiated colony appearance. The colonies grown on mEFs and human fetal fibroblasts expressed SSEA-4 and alkaline phosphatase surface markers and positive for the expression of Oct-4 by reverse transcription-polymerase chain reaction (RT-PCR). The produced embryoid body differentiated spontaneously to neural progenitorlike cells, neuron-like cells and beating cardiomyocyte-like cells, and frozen-thawed embryonic stem cells displayed normal 46,XX karyotype. Conclusions: The human embryonic stem cells can be established by using mEFs and human fetal fibroblasts produced in laboratory as feeder cells.

• 한국작물학회지
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• 제47권5호
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• pp.374-378
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• 2002

To evaluate growth habits, fresh pod yield potential, and possibility of early and late seeding, seeding dates were extended from March 21 to June 20 by PE mulching and non-mulching. Soil temperature, under 5cm from surface, above 15$^{\circ}C$ at 10 a.m. in early seeding reached about March 25 in mulching and April 5 to April 12 in non-mulching. Days to emergence and first flowering were accelerated owing to increasing temperature, as seeding was delayed. Days to emergence according to seeding dates reduced 21 to 8 day in mulching and 33 to 10 day in non-mulching. Days to flowering were ranged from 51 to 26 day in mulching and from 69 to 32 day in non-mulching and differences between mulching and non-mulching on each seeding date had 18 to 4 days. Early seedings till April 21 had 160-170 flowers per plant for 8 weeks, while late seedings from May 21 increased more speedily with 200 flower for 6 weeks. Harvesting of fresh peanut, at 80 days after first flowering, was possible from Aug. 1 to Oct. 7 (133-108 days to harvest) by mulching and from Aug. 19 to Oct. 12 (151 to 114 days) by non-mulching. Yields between mulching and non-mulching in early seeding until April 21 had more difference, but in late seeding after May 21 was higher and showed insignificance. Pod setting periods by early and late seeding were about 3 weeks equally. In late seeding pod setting were almost concentrated for front 15 days. In spite of difference of fresh pod weight between two seeding times, the distributions of average of seed weight showed nearly same tendency.

• 대한물리치료과학회지
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• 제3권3호
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• pp.93-104
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• 1996

The purpose of this study is improvement of clinical practice education through examination of satisfaction on clinical practice of physical therapy students and grasping the factor have on effect on satisfaction of clinical practice. The students of the survey were 328 junior college students and 41 University students selected from 8 junior college and 2 University by questionnaire from Oct. 6 to Oct. 26, 1993. The collected data was analyzed by ANOVA, T-test, Multiple range test (Tukey, LSD, Duncan), principal factor analysis according to purpose of study. The results were as follows : The degree of satisfaction according to the item on clinical practice was low as 0.346 of a total 1.0. The degree of satisfaction of instruction(0.40) revealed to be high. Compared with the degree of satisfaction on contents(0.39), practice environment(0.38), satisfaction after practice(0.36), practice hours(0.35), personalrelations(0.33), problem of practice place(0.31), practice evaluation(0.25) revealed to be low. 1. The degree of satisfaction on clinical practice according to education system was lower junior college than University. 2. The degree of satisfaction on clinical practice according to volitional group and another intention group and another intention group was not different. 3. The degree of satisfaction on clinical practice according to the order of merit was not difference A group, B group, C group, but it of D group was low. 4. The degree of satisfaction on clinical practice according to the time of practise, the group that clinical enforce during a session was higher than the group that it enforce during vacation. 5. The degree of satisfaction on clinical practice according to clinical practice place was high in University hospital and general hospital, but was low in hospital and clinic. 6. The degree of satisfaction on clinical practice according to existence or non-existence of a hospital in affiliation was higher existence a hospital in affiliation group than non-existencea hospital in affiliation group. 7. The degree of satisfaction on clinical practice according to satisfaction of major was high in group of high satisfaction major.

• Journal of the Optical Society of Korea
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• 제15권3호
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• pp.293-299
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• 2011

In this study, we demonstrated Fourier-domain/swept-source optical coherence tomography (FD/SS-OCT) at a center wavelength of 800 nm for in vivo human retinal imaging. A wavelength-swept source was constructed with a semiconductor optical amplifier, a fiber Fabry-Perot tunable filter, isolators, and a fiber coupler in a ring cavity. Our swept source produced a laser output with a tuning range of 42 nm (779 to 821 nm) and an average power of 3.9 mW. The wavelength-swept speed in this configuration with bidirectionality is 2,000 axial scans per second. In addition, we suggested a modified zero-crossing method to achieve equal sample spacing in the wavenumber (k) domain and to increase the image depth range. FD/SS-OCT has a sensitivity of ~89.7 dB and an axial resolution of 10.4 ${\mu}m$ in air. When a retinal image with 2,000 A-lines/frame is obtained, an acquisition speed of 2.0 fps is achieved.

• 한국작물학회지
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• 제39권5호
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• pp.431-436
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• 1994

충남 예산지방에서 호밀을 답리작으로 재배할 때 파종기가 생육, 수량 및 식물체 부위별 건물구 성비율에 미치는 영향을 요약하면 다음과 같다. 1. 예산지방에서 파종기가 11월 5일 이후로 늦어지면 호밀의 출현일수가 길어지고 출현율 및 월동율이 감소하며 출수기가 지연되었다. 2. 호밀의 생장속도는 파종기가 빠를수록 증가하였고 파종기가 11월 5일 이후로 지연됨에 따라 생육량이 크게 감소하였다. 3. 호밀의 청예 및 건물수량은 파종기가 빠를수록 증가하나 다른 지역에서보다 파종적기의 폭이 넓어서 10월 5일에서 10월 25일 사이의 파종기간 차가 크지 않았다. 청예수량은 개화기 예취시, 건물수량은 유숙기 예취시 가장 높았다. 4. 호밀의 식물체 부위별 건물구성비율은 파종기가 빠를수록 그리고 수확시기가 늦을수록 엽신과 엽초의 비율이 감소하고 줄기와 이삭의 비율은 증가하였다.

• 한국초지조사료학회지
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• 제7권1호
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• pp.18-24
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• 1987

This experiment was carried out to investigate the effects of the final cutting time and cutting height on the winter survival, regrowth, and carbohydrate reserves in stubble, and early spring yield of orchardgrass-dominated pasture before and after the winter season. The experiment was conducted at the experimental field of the Livestock Experiment Station, in Suwon, from October 1985 to early 1986. The results obtained are summarized as follows: 1. Regrowth of plant after the final cut increased significantly (P<0.05) with plot of the early cutting time (Oct. 10) and high cutting height at the final. For winter survival, cut plant should regrow over 15cm in plant height. Therefore limit cutting time and cutting height were forced to be on Oct. 25 and at 6-9cm, respectively. 2. Total water soluble carbohydrate (TSC) content in stubble after the final cut was not significantly changed by different cutting time and cutting height. 3. The percentage of dead plant after wintering was found to be high with plot of the late cutting time and low cutting height at the final cut (P<0.05). And the correlation between the percentage of dead plant after wintering and final plant height before wintering was significantly negathe (r = -0.728**). 4. Fresh and DM yield at the early spring after wintering were increased in the plot of the early cutting time and high cutting height (P<0.05) at the final cut. And significantly positive correlation was observed between the early spring yield and final plant height before wintering (r = 0.720**).