• Journal of Practical Agriculture & Fisheries Research
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• v.19 no.1
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• pp.75-82
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• 2017

This experiment was carried out to establish growth characterestics and number of bulblet, bulb weight, and diameter of bulblet on scale tissue culture in Lilium brownii. Flowering date of Lilium brownii was 25th, May with 50cm hight in field condition. Vimyl house condition was flowered 28th May with 54cm hight. Number of bulblet, bulb weight, diameter of bublet was good position of outer>Middle>inner scale in Lilium brownii. Number of bulblet formation was the best with 3.5 in large scale. Concentration of BA was the best at 1.5mg/L with 3.8 bulblet with bulb weight, weight and diameter of bulblet.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.341-346
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• 2001

To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.358-360
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• 1998

Series of in vitro experiments in Lilium callosum were conducted to investigate efficient multiplication through finding the optimal cultural environment, and organogenic capability of cultural explants, and then to determine the progressive method for enhancing bulblet growth in Lilium callosum scale culture. Twenty-four hr photoperiod was most effective for the growth of bulblet and the formation of other organs. Optimum light intensity for bulblet growth was 2,500~5,000 Lux. When bulbets were subcultured, growth of bulblets were enhanced by removing excessive leaf blade. Number of bulblets per scale increased as mother scale size increased, whereas diameter of bulblet from the small size mother scale increased. Bulblet formation and development was induced when explants were placed above the medium to be exposed to more light.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.139-143
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• 2004

The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30g/L, and the formation of bulblets was strongly inhibited on medium containing over 90g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95％.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.373-380
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• 2017

Amaryllis ($Hippeastrum{\times}johnsonii$) is commonly propagated by three methods: seeds, offset bulblets, and twin scaling. Since the number and size of bulblets produced by these methods are low, we conducted an experiment to evaluate different bulb cutting methods for propagation. For this purpose, bulbs (circumference of 30 - 35 cm) were sectioned or notched into 8, 12, or 16 segments or twin-scaled into 48, 72, or 96 segments for bulblet formation. Our results show that the largest number of bulblets was produced by the twin scaling method, but they take longer to grow to a final, commercial size. Sectioning and notching resulted in larger, but fewer bulblets than twin-scaling. Compared to notching, sectioning provided more space for the bulblets to grow, and is therefore the recommended method. While increasing the number of sections cut from a single bulb resulted in a larger number of bulblets, the diameter of the bulblets decreased. Therefore, sectioning the bulb into 8 segments was the best method for producing an acceptable number of vigorous bulblets.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.154-161
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• 1994

This experiment was carried out to establish micropropagation system in Fritillaria thunbergii Miq. Through the culture of bulblet scales, stems, node-buds and shoot tips with special reference to the effect of physiological age of explant and plant growth regulators on bulblet formation. Number of formed bulblets was significantly increased in node-bud or stem tissue compared to scals segments and on the medium supplemented with kinetin than BA containing medium. Optimum levels of kinetin for bulblet formation from node-bud taken from above 3 cm shoot length and stem segments excised from below 3 cm shoot length were 5.0 mg /L and $1.0{\sim}3.0\;mg$ /L kinetin, respectively. Interesting phenomenon was observed, the direct formation of bulblets from the axilliary bud of cultured explants. Bulblet forming capacity in stem tissue was depended on stem age, young stem had high regeneration ability compared to old stem taken from above 10 cm shoot length. 1.0 mg /L kinetin was optimum concentration for the formation of bulblets from old stem segments. Stem tissue taken from underground growing plant was promoted coampare to shoot tips or bulb scale segments. Optimum concentration of sucrose was $5{\sim}7%$. Summariged above results revealed that effective explant for micropropagation was stem and /or node-bud tissue excised from less than 3 cm plant height compared to those of bulb scale segments which showed high contamination after culture. Maximum multiplication rate of young stem and /or node-bud segment was about 20 times. Kinetin requirement for stimulation of bulblet formation from cultured explant depended on source of explants but favorable levels of kinetin for organogenesis ranged from 1.0 mg /L to 5.0 mg /L.

• Horticultural Science & Technology
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• v.16 no.2
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• pp.242-243
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• 1998

This research was conducted to find the most effective and economical artificial propagation method of Lycoris squamigera among chipping, half-chipping, twin-scaling, coring, scooping and notching, in order to investigate the number of bulblets obtained from one bulb and the number of bulbs treated by a man in a day for artificial propagation. We got 42.2 bulblets from one bulb by twin-scaling, 23.2 bulblets by half-chipping, 18.2 bulblets by chipping, 14.3 bulblets by notching, 1 bulblet by coring and also 1 bulblet by scooping. Although we got the most bulblets by twin-scaling, it took the most time to treat the bulbs for artificial propagation (200 bulbs per day) and the bulblets formed were very small. But 400 bulbs were treated in a day by chipping and the bulblets formed were comparatively large. In addition, machines could be used for chipping, so chipping was considered to be the most effective and economical artificial propagation method.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.25-29
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• 2000

Liquid shaking culture was conducted to investigate the proper culture conditions for the micropropagation of high quality lily using bulblets (3 mm in diameter) obtained from small scale culture. The combinations of 9% sucrose and 10 mM nitrogen or 6% sucrose and 20 mM $NH_4NO_3$ were effective on the growth and weight of micro-bulbs. However, the number of new bulbs was the highest when 20 to 40 mM $NH_4NO_3$ and 3% sucrose were added to the MS medium. The total fresh weight was increased effectively in MS medium supplemented with BA 0.2 mg/L alone under $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity. Also bulblet weight was increased at $60\;{\mu}mol{\cdot}m^2{\cdot}sec^{-1}$ intensity, regardless of BA concentrations (0.2 and 2 mg/L) in the medium. The proper culture period of bulblet was about 2 month in liquid shaking culture.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.124-125
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• 2008

• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.197-201
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• 2004

Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.