Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micropropagation of Lilium longiflorum 'Geogia' by Using Bioreactor.

생물반응기를 이용한 Lilium longiflorum ′Geogil′의 대량번식

  • 한봉희 (원예연구소 원예생명공학과) ;
  • 서은정 (원예연구소 원예생명공학과) ;
  • 예병우 (농촌진흥청 연구관리과) ;
  • 유희주 (원예연구소 원예생명공학과)
  • Published : 2004.09.01
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Abstract

Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.

자구를 형성 및 비대시키기 위하여 BA 0.5 mg/L와 IAA 0.5 mg/L가 첨가된 MS 배지에서 Lilium longiflorum 'Geogia'의 신초를 증식하였다. 증식된 신초 cluster를 5 L air lift 생물반응기에 배양하였다. 자구비대는 sucrose 60 g/L 및 1∼2배 사이의 MS 배지 염류농도에서 효과적이었다. 재료 투입량은 100 g을 투입하는 것이 자구형성 및 자구비대에 적절하였다. 배양방법은 air-lift 방법이 자구의 형성 및 비대를 촉진하였으며, 주입공기량은 200∼300 mL$.$$min^{-1}$을 주입하는 것이 자구비대에 효과적이었다.

Keywords

References

  1. Gerrits MM, De Klerk GJ (1992) Dry-matter partitioning between bulb and leaves in plantlets of Lilium speciosum regenerated in vitro. Acta Bot Neerl 41: 461-468 https://doi.org/10.1111/j.1438-8677.1992.tb00516.x
  2. Han BH, Yae BW, Goo DH, Ko JY (1999a) The formation and growth of bulblets from bulblet sections with swollen basal plate in Lilium Oriental Hybrid 'Casa Blanca'. J Kor Soc Hort Sci 40: 747-750
  3. Han BH, Yae BW, Goo DH, Ko JY (1999b) Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulbscales in Lilium Oriental Hybrid 'Casa Blanca'. Kor J Plant Tiss Cult 26: 103-107
  4. Lian ML, Chakrabarty D, Paek KY (2002) growth of Lilium oriental hybrid 'Casablanca' bulblet using bioreactor culture. Scientia Hort 97: 41-48 https://doi.org/10.1016/S0304-4238(02)00086-9
  5. Maesato K, Sharada K, Fukui H, Hara T, Sarma KS (1994) In vitro bulblet regeneration from bulbscale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment. J Hort Sci 69: 289-297
  6. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  7. Nhut DT (1998) Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo bulblet culture. Plant Cell Rep 17: 913-916 https://doi.org/10.1007/s002990050508
  8. Niimi Y (1984) Bulblet productivity of explants from scales, leaves, stems and tepals of Lilium rubellum Barker. Scientia Hort 22: 391-394 https://doi.org/10.1016/S0304-4238(84)80011-4
  9. Niimi Y (1986) Applicatrion of leaf segment culture to in vitrro bulblets production of six Lilium species. Acta Bot 35: 189-194
  10. Niimi Y, Onozawa T (1977) Vegetative propagation of L. rubellum Barker; Comperison of bulblet formation between scaling and scale segment cultured in vitro. Abstracts Fall Meeting. Jap Soc Hort Sci pp. 348-349
  11. Novak FJ, Petru E (1981) Tissue culture propagation of Lilium hybrids. Scientia Hort 14: 191-199 https://doi.org/10.1016/0304-4238(81)90012-1
  12. Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large scale micropropagation systems of plants. In vitro Cell. Dev. Biol. Plant. 37:284-292
  13. Priyadarshi S, Sen S (1992) A revised scheme for mass propagation of Easter Lily. Plant Cell Tiss Organ Cult 30: 193-197 https://doi.org/10.1007/BF00040021
  14. Stimart DP, Ascher PD (1978) Tissue culture of bulb-scale sections for asexual propagation of Lilium longiflorum Thunb. J Amer Soc Hort Sci 103: 182-184
  15. Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiol Plant 46: 184-190 https://doi.org/10.1111/j.1399-3054.1979.tb06555.x
  16. Takayama S, Misawa M (1980) Differentiation in Lilium bulbscales grown in vitro. Effect of activated charcoal, physiological age of bulbs and sucrose concentrations on differentiation and scale leaf formation in vitro. Physiol Plant 48: 121-125 https://doi.org/10.1111/j.1399-3054.1980.tb03229.x
  17. Takayama S, Misawa M (1982) Regulation of organ formation by cytokinin and auxin in Lilium bulbscales grown in vitro. Plant Cell Physiol 23: 67-74 https://doi.org/10.1093/oxfordjournals.pcp.a076331
  18. Takayama S, Misawa M (1983) The mass propagation of Lilium in vitro by stimulation of multiple adventitious bulb-scale formation and by shake culture. Can J Bot 61: 224-228 https://doi.org/10.1139/b83-025
  19. Tanimoto S, Matsubara Y (1995) Stimulating effect of spermine on bulblet formation in bulb-scale segments of Lilium longiflorum. Plant Cell Rep 15: 297-300 https://doi.org/10.1007/BF00193740
  20. Wickremesinhe ERM, Holcomb EJ, Arteca RN (1994) A practical mrthod for the production of flowering Easter lilies from callus cultures. Sci Hort 60: 143-152 https://doi.org/10.1016/0304-4238(94)90069-8